Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11520 and EX31_RS11525 are separated by 115 nucleotides EX31_RS11525 and EX31_RS11530 are separated by 462 nucleotides
EX31_RS11520: EX31_RS11520 - glucose-1-phosphate thymidylyltransferase RfbA, at 241,212 to 242,084
_RS11520
EX31_RS11525: EX31_RS11525 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 242,200 to 243,087
_RS11525
EX31_RS11530: EX31_RS11530 - TerC family protein, at 243,550 to 245,139
_RS11530
Position (kb) 242
243
244 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 242.078 kb on + strand at 242.078 kb on + strand at 242.078 kb on + strand at 242.078 kb on + strand at 242.078 kb on + strand at 242.078 kb on + strand at 242.078 kb on + strand at 242.078 kb on + strand at 242.086 kb on + strand at 242.088 kb on + strand at 242.088 kb on + strand at 242.088 kb on + strand at 242.088 kb on + strand at 242.122 kb on + strand at 242.133 kb on + strand at 242.178 kb on - strand at 242.289 kb on - strand, within EX31_RS11525 at 242.299 kb on - strand, within EX31_RS11525 at 242.347 kb on - strand, within EX31_RS11525 at 242.406 kb on - strand, within EX31_RS11525 at 242.406 kb on - strand, within EX31_RS11525 at 242.434 kb on - strand, within EX31_RS11525 at 242.509 kb on - strand, within EX31_RS11525 at 242.546 kb on - strand, within EX31_RS11525 at 242.566 kb on - strand, within EX31_RS11525 at 242.571 kb on - strand, within EX31_RS11525 at 242.571 kb on - strand, within EX31_RS11525 at 242.571 kb on - strand, within EX31_RS11525 at 242.728 kb on - strand, within EX31_RS11525 at 242.789 kb on - strand, within EX31_RS11525 at 242.979 kb on - strand, within EX31_RS11525 at 243.045 kb on + strand at 243.046 kb on - strand at 243.046 kb on - strand at 243.046 kb on - strand at 243.046 kb on - strand at 243.046 kb on - strand at 243.046 kb on - strand at 243.091 kb on - strand at 243.112 kb on - strand at 243.135 kb on - strand at 243.218 kb on - strand at 243.218 kb on - strand at 243.218 kb on - strand at 243.219 kb on + strand at 243.220 kb on - strand at 243.220 kb on - strand at 243.220 kb on - strand at 243.220 kb on - strand at 243.220 kb on - strand at 243.220 kb on - strand at 243.220 kb on - strand at 243.220 kb on - strand at 243.249 kb on + strand at 243.250 kb on - strand at 243.250 kb on - strand at 243.261 kb on + strand at 243.261 kb on + strand at 243.261 kb on + strand at 243.279 kb on - strand at 243.279 kb on - strand at 243.279 kb on - strand at 243.279 kb on - strand at 243.279 kb on - strand at 243.279 kb on - strand at 243.283 kb on + strand at 243.284 kb on - strand at 243.284 kb on - strand at 243.293 kb on - strand at 243.293 kb on - strand at 243.304 kb on - strand at 243.321 kb on - strand at 243.326 kb on - strand at 243.350 kb on + strand at 243.351 kb on - strand at 243.376 kb on - strand at 243.376 kb on - strand at 243.413 kb on - strand at 243.459 kb on - strand at 243.502 kb on + strand at 243.600 kb on - strand at 243.725 kb on + strand, within EX31_RS11530 at 243.725 kb on + strand, within EX31_RS11530 at 243.816 kb on + strand, within EX31_RS11530 at 243.817 kb on - strand, within EX31_RS11530 at 243.833 kb on + strand, within EX31_RS11530 at 243.864 kb on + strand, within EX31_RS11530 at 243.918 kb on + strand, within EX31_RS11530 at 243.918 kb on + strand, within EX31_RS11530 at 244.035 kb on + strand, within EX31_RS11530 at 244.035 kb on + strand, within EX31_RS11530 at 244.036 kb on - strand, within EX31_RS11530 at 244.036 kb on - strand, within EX31_RS11530
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 242,078 + +1.2 242,078 + -0.8 242,078 + +1.8 242,078 + +1.9 242,078 + -0.1 242,078 + -0.6 242,078 + -0.0 242,078 + +0.6 242,086 + +1.5 242,088 + -0.1 242,088 + -0.6 242,088 + +2.2 242,088 + -0.2 242,122 + +0.2 242,133 + +3.2 242,178 - +0.5 242,289 - EX31_RS11525 0.10 -1.0 242,299 - EX31_RS11525 0.11 +0.6 242,347 - EX31_RS11525 0.17 +1.6 242,406 - EX31_RS11525 0.23 -0.6 242,406 - EX31_RS11525 0.23 -0.1 242,434 - EX31_RS11525 0.26 -0.1 242,509 - EX31_RS11525 0.35 -0.1 242,546 - EX31_RS11525 0.39 +0.2 242,566 - EX31_RS11525 0.41 -0.1 242,571 - EX31_RS11525 0.42 +0.6 242,571 - EX31_RS11525 0.42 +0.6 242,571 - EX31_RS11525 0.42 +1.2 242,728 - EX31_RS11525 0.59 -1.5 242,789 - EX31_RS11525 0.66 -0.1 242,979 - EX31_RS11525 0.88 -0.1 243,045 + +1.2 243,046 - +0.2 243,046 - +0.2 243,046 - -0.4 243,046 - -1.4 243,046 - -0.8 243,046 - -1.0 243,091 - +0.2 243,112 - +0.4 243,135 - -0.5 243,218 - -1.3 243,218 - -0.6 243,218 - +1.2 243,219 + +1.0 243,220 - -0.7 243,220 - +0.1 243,220 - +2.2 243,220 - -0.1 243,220 - +1.1 243,220 - +1.2 243,220 - -0.7 243,220 - +2.9 243,249 + +0.7 243,250 - -0.5 243,250 - +0.4 243,261 + +0.2 243,261 + +1.3 243,261 + +0.6 243,279 - -0.1 243,279 - -1.7 243,279 - -1.8 243,279 - -0.5 243,279 - +1.2 243,279 - -0.8 243,283 + +0.9 243,284 - +0.5 243,284 - +0.6 243,293 - +0.4 243,293 - +0.9 243,304 - -0.8 243,321 - +1.2 243,326 - -0.4 243,350 + +0.1 243,351 - +0.5 243,376 - -0.6 243,376 - +0.0 243,413 - -0.1 243,459 - +0.9 243,502 + +0.9 243,600 - +0.6 243,725 + EX31_RS11530 0.11 +1.1 243,725 + EX31_RS11530 0.11 +0.9 243,816 + EX31_RS11530 0.17 +0.5 243,817 - EX31_RS11530 0.17 -0.5 243,833 + EX31_RS11530 0.18 -0.5 243,864 + EX31_RS11530 0.20 +0.0 243,918 + EX31_RS11530 0.23 -0.4 243,918 + EX31_RS11530 0.23 -0.5 244,035 + EX31_RS11530 0.31 +3.2 244,035 + EX31_RS11530 0.31 -1.1 244,036 - EX31_RS11530 0.31 +1.2 244,036 - EX31_RS11530 0.31 +2.2
Or see this region's nucleotide sequence