Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11410 and EX31_RS11415 overlap by 4 nucleotides EX31_RS11415 and EX31_RS11420 are separated by 176 nucleotides EX31_RS11420 and EX31_RS11425 are separated by 2 nucleotides
EX31_RS11410: EX31_RS11410 - flagellar motor protein MotB, at 215,384 to 216,487
_RS11410
EX31_RS11415: EX31_RS11415 - flagellar motor stator protein MotA, at 216,484 to 217,374
_RS11415
EX31_RS11420: EX31_RS11420 - flagellar transcriptional regulator FlhC, at 217,551 to 218,159
_RS11420
EX31_RS11425: EX31_RS11425 - flagellar transcriptional regulator FlhD, at 218,162 to 218,506
_RS11425
Position (kb) 216
217
218 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 215.577 kb on - strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.603 kb on + strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.604 kb on - strand, within EX31_RS11410 at 215.710 kb on + strand, within EX31_RS11410 at 215.711 kb on - strand, within EX31_RS11410 at 215.711 kb on - strand, within EX31_RS11410 at 215.865 kb on + strand, within EX31_RS11410 at 215.887 kb on - strand, within EX31_RS11410 at 215.903 kb on - strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.976 kb on + strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 215.977 kb on - strand, within EX31_RS11410 at 216.143 kb on + strand, within EX31_RS11410 at 216.144 kb on - strand, within EX31_RS11410 at 216.163 kb on + strand, within EX31_RS11410 at 216.164 kb on - strand, within EX31_RS11410 at 216.247 kb on + strand, within EX31_RS11410 at 216.304 kb on - strand, within EX31_RS11410 at 216.386 kb on - strand at 216.386 kb on - strand at 216.386 kb on - strand at 216.440 kb on - strand at 216.440 kb on - strand at 216.455 kb on + strand at 216.455 kb on + strand at 216.456 kb on - strand at 216.456 kb on - strand at 216.456 kb on - strand at 216.487 kb on + strand at 216.488 kb on - strand at 216.488 kb on - strand at 216.488 kb on - strand at 216.488 kb on - strand at 216.556 kb on - strand at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.578 kb on + strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.579 kb on - strand, within EX31_RS11415 at 216.695 kb on - strand, within EX31_RS11415 at 216.816 kb on + strand, within EX31_RS11415 at 216.816 kb on + strand, within EX31_RS11415 at 216.817 kb on - strand, within EX31_RS11415 at 216.817 kb on - strand, within EX31_RS11415 at 216.820 kb on + strand, within EX31_RS11415 at 216.820 kb on + strand, within EX31_RS11415 at 216.820 kb on + strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.821 kb on - strand, within EX31_RS11415 at 216.914 kb on + strand, within EX31_RS11415 at 216.915 kb on - strand, within EX31_RS11415 at 216.915 kb on - strand, within EX31_RS11415 at 216.915 kb on - strand, within EX31_RS11415 at 216.989 kb on + strand, within EX31_RS11415 at 217.149 kb on + strand, within EX31_RS11415 at 217.150 kb on - strand, within EX31_RS11415 at 217.150 kb on - strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.151 kb on + strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.152 kb on - strand, within EX31_RS11415 at 217.161 kb on + strand, within EX31_RS11415 at 217.161 kb on + strand, within EX31_RS11415 at 217.166 kb on + strand, within EX31_RS11415 at 217.289 kb on + strand at 217.289 kb on + strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.290 kb on - strand at 217.355 kb on + strand at 217.355 kb on + strand at 217.355 kb on + strand at 217.356 kb on - strand at 217.360 kb on + strand at 217.361 kb on - strand at 217.361 kb on - strand at 217.361 kb on - strand at 217.364 kb on - strand at 217.386 kb on - strand at 217.386 kb on - strand at 217.460 kb on - strand at 217.460 kb on - strand at 217.533 kb on + strand at 217.534 kb on - strand at 217.534 kb on - strand at 217.547 kb on + strand at 217.548 kb on - strand at 217.591 kb on + strand at 217.593 kb on + strand at 217.593 kb on + strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.594 kb on - strand at 217.595 kb on + strand at 217.595 kb on + strand at 217.596 kb on - strand at 217.596 kb on - strand at 217.596 kb on - strand at 217.619 kb on + strand, within EX31_RS11420 at 217.620 kb on - strand, within EX31_RS11420 at 217.698 kb on - strand, within EX31_RS11420 at 217.698 kb on - strand, within EX31_RS11420 at 217.698 kb on - strand, within EX31_RS11420 at 217.698 kb on - strand, within EX31_RS11420 at 217.719 kb on - strand, within EX31_RS11420 at 217.719 kb on - strand, within EX31_RS11420 at 217.758 kb on + strand, within EX31_RS11420 at 217.759 kb on - strand, within EX31_RS11420 at 217.759 kb on - strand, within EX31_RS11420 at 217.759 kb on - strand, within EX31_RS11420 at 217.761 kb on + strand, within EX31_RS11420 at 217.761 kb on + strand, within EX31_RS11420 at 217.761 kb on + strand, within EX31_RS11420 at 217.829 kb on - strand, within EX31_RS11420 at 217.830 kb on + strand, within EX31_RS11420 at 217.831 kb on - strand, within EX31_RS11420 at 217.837 kb on + strand, within EX31_RS11420 at 217.865 kb on + strand, within EX31_RS11420 at 217.866 kb on - strand, within EX31_RS11420 at 217.866 kb on - strand, within EX31_RS11420 at 217.895 kb on - strand, within EX31_RS11420 at 217.953 kb on - strand, within EX31_RS11420 at 218.003 kb on - strand, within EX31_RS11420 at 218.003 kb on - strand, within EX31_RS11420 at 218.010 kb on - strand, within EX31_RS11420 at 218.029 kb on - strand, within EX31_RS11420 at 218.029 kb on - strand, within EX31_RS11420 at 218.029 kb on - strand, within EX31_RS11420 at 218.029 kb on - strand, within EX31_RS11420 at 218.029 kb on - strand, within EX31_RS11420 at 218.084 kb on + strand, within EX31_RS11420 at 218.118 kb on - strand at 218.150 kb on + strand at 218.151 kb on - strand at 218.159 kb on + strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.160 kb on - strand at 218.164 kb on - strand at 218.164 kb on - strand at 218.164 kb on - strand at 218.164 kb on - strand at 218.252 kb on + strand, within EX31_RS11425 at 218.252 kb on + strand, within EX31_RS11425 at 218.252 kb on + strand, within EX31_RS11425 at 218.253 kb on - strand, within EX31_RS11425 at 218.343 kb on - strand, within EX31_RS11425
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 215,577 - EX31_RS11410 0.17 -0.7 215,603 + EX31_RS11410 0.20 -0.6 215,603 + EX31_RS11410 0.20 -0.7 215,603 + EX31_RS11410 0.20 -0.5 215,603 + EX31_RS11410 0.20 +0.1 215,604 - EX31_RS11410 0.20 +0.2 215,604 - EX31_RS11410 0.20 +0.6 215,604 - EX31_RS11410 0.20 -0.1 215,604 - EX31_RS11410 0.20 +0.7 215,604 - EX31_RS11410 0.20 -0.7 215,604 - EX31_RS11410 0.20 -1.1 215,604 - EX31_RS11410 0.20 -1.3 215,710 + EX31_RS11410 0.30 -0.3 215,711 - EX31_RS11410 0.30 +1.0 215,711 - EX31_RS11410 0.30 -1.1 215,865 + EX31_RS11410 0.44 -0.4 215,887 - EX31_RS11410 0.46 -0.7 215,903 - EX31_RS11410 0.47 -2.0 215,976 + EX31_RS11410 0.54 -1.1 215,976 + EX31_RS11410 0.54 -1.8 215,976 + EX31_RS11410 0.54 -0.4 215,977 - EX31_RS11410 0.54 -1.6 215,977 - EX31_RS11410 0.54 +0.6 215,977 - EX31_RS11410 0.54 -2.0 215,977 - EX31_RS11410 0.54 -0.1 216,143 + EX31_RS11410 0.69 -1.6 216,144 - EX31_RS11410 0.69 -0.3 216,163 + EX31_RS11410 0.71 -2.0 216,164 - EX31_RS11410 0.71 -0.9 216,247 + EX31_RS11410 0.78 +0.5 216,304 - EX31_RS11410 0.83 -0.6 216,386 - -1.0 216,386 - -1.3 216,386 - -0.6 216,440 - -0.1 216,440 - +0.9 216,455 + +0.4 216,455 + -0.6 216,456 - +0.2 216,456 - -1.3 216,456 - -1.3 216,487 + +0.2 216,488 - -0.5 216,488 - -0.7 216,488 - -2.6 216,488 - -0.7 216,556 - -0.8 216,578 + EX31_RS11415 0.11 -2.3 216,578 + EX31_RS11415 0.11 -1.4 216,578 + EX31_RS11415 0.11 +0.2 216,578 + EX31_RS11415 0.11 +2.2 216,578 + EX31_RS11415 0.11 +2.2 216,578 + EX31_RS11415 0.11 -3.1 216,579 - EX31_RS11415 0.11 +0.1 216,579 - EX31_RS11415 0.11 +0.6 216,579 - EX31_RS11415 0.11 +0.3 216,579 - EX31_RS11415 0.11 -2.3 216,579 - EX31_RS11415 0.11 +0.2 216,579 - EX31_RS11415 0.11 -0.1 216,695 - EX31_RS11415 0.24 +0.3 216,816 + EX31_RS11415 0.37 +0.4 216,816 + EX31_RS11415 0.37 -0.8 216,817 - EX31_RS11415 0.37 -0.3 216,817 - EX31_RS11415 0.37 -1.5 216,820 + EX31_RS11415 0.38 -0.1 216,820 + EX31_RS11415 0.38 +0.4 216,820 + EX31_RS11415 0.38 -1.1 216,821 - EX31_RS11415 0.38 +3.8 216,821 - EX31_RS11415 0.38 +2.2 216,821 - EX31_RS11415 0.38 -1.4 216,821 - EX31_RS11415 0.38 -0.8 216,821 - EX31_RS11415 0.38 +0.4 216,821 - EX31_RS11415 0.38 -2.0 216,821 - EX31_RS11415 0.38 -0.9 216,821 - EX31_RS11415 0.38 +0.4 216,821 - EX31_RS11415 0.38 -0.7 216,821 - EX31_RS11415 0.38 -1.3 216,821 - EX31_RS11415 0.38 +1.0 216,821 - EX31_RS11415 0.38 -1.3 216,914 + EX31_RS11415 0.48 +1.6 216,915 - EX31_RS11415 0.48 -0.4 216,915 - EX31_RS11415 0.48 -0.0 216,915 - EX31_RS11415 0.48 -0.6 216,989 + EX31_RS11415 0.57 -0.5 217,149 + EX31_RS11415 0.75 +0.3 217,150 - EX31_RS11415 0.75 -1.0 217,150 - EX31_RS11415 0.75 +0.6 217,151 + EX31_RS11415 0.75 -0.5 217,151 + EX31_RS11415 0.75 -1.6 217,151 + EX31_RS11415 0.75 -1.8 217,151 + EX31_RS11415 0.75 -1.4 217,151 + EX31_RS11415 0.75 +0.4 217,151 + EX31_RS11415 0.75 -0.1 217,152 - EX31_RS11415 0.75 +0.0 217,152 - EX31_RS11415 0.75 -0.8 217,152 - EX31_RS11415 0.75 -0.1 217,152 - EX31_RS11415 0.75 +1.7 217,152 - EX31_RS11415 0.75 -1.4 217,152 - EX31_RS11415 0.75 -1.6 217,152 - EX31_RS11415 0.75 -1.0 217,152 - EX31_RS11415 0.75 -0.5 217,152 - EX31_RS11415 0.75 -1.4 217,152 - EX31_RS11415 0.75 -1.7 217,161 + EX31_RS11415 0.76 +0.6 217,161 + EX31_RS11415 0.76 +1.6 217,166 + EX31_RS11415 0.77 -1.6 217,289 + +1.6 217,289 + +0.2 217,290 - +0.9 217,290 - -1.5 217,290 - +1.2 217,290 - -1.1 217,290 - +0.6 217,290 - -0.1 217,290 - -0.1 217,290 - -0.2 217,355 + -0.1 217,355 + +0.2 217,355 + -0.5 217,356 - -2.0 217,360 + +1.5 217,361 - -1.8 217,361 - -0.4 217,361 - -2.0 217,364 - -1.0 217,386 - +0.1 217,386 - +1.3 217,460 - -0.3 217,460 - -0.4 217,533 + -0.1 217,534 - -0.6 217,534 - -1.5 217,547 + +0.9 217,548 - -0.3 217,591 + -1.7 217,593 + -0.1 217,593 + -2.3 217,594 - -0.3 217,594 - -1.8 217,594 - -1.1 217,594 - -0.3 217,594 - -1.3 217,594 - -2.4 217,594 - +0.3 217,594 - -1.5 217,594 - -1.4 217,594 - -0.1 217,594 - +0.1 217,594 - +0.0 217,594 - +0.0 217,594 - +0.3 217,594 - -0.4 217,595 + -0.8 217,595 + -0.8 217,596 - -0.3 217,596 - +0.2 217,596 - -0.1 217,619 + EX31_RS11420 0.11 -1.9 217,620 - EX31_RS11420 0.11 +0.5 217,698 - EX31_RS11420 0.24 -0.4 217,698 - EX31_RS11420 0.24 -0.6 217,698 - EX31_RS11420 0.24 -1.4 217,698 - EX31_RS11420 0.24 -1.5 217,719 - EX31_RS11420 0.28 -1.1 217,719 - EX31_RS11420 0.28 -2.3 217,758 + EX31_RS11420 0.34 +0.4 217,759 - EX31_RS11420 0.34 -0.1 217,759 - EX31_RS11420 0.34 -1.1 217,759 - EX31_RS11420 0.34 -2.1 217,761 + EX31_RS11420 0.34 +2.2 217,761 + EX31_RS11420 0.34 -0.7 217,761 + EX31_RS11420 0.34 +1.2 217,829 - EX31_RS11420 0.46 -0.4 217,830 + EX31_RS11420 0.46 +0.3 217,831 - EX31_RS11420 0.46 -0.6 217,837 + EX31_RS11420 0.47 -1.0 217,865 + EX31_RS11420 0.52 -1.5 217,866 - EX31_RS11420 0.52 -0.1 217,866 - EX31_RS11420 0.52 -0.9 217,895 - EX31_RS11420 0.56 -1.4 217,953 - EX31_RS11420 0.66 -0.4 218,003 - EX31_RS11420 0.74 -0.6 218,003 - EX31_RS11420 0.74 -0.1 218,010 - EX31_RS11420 0.75 -0.5 218,029 - EX31_RS11420 0.78 -1.2 218,029 - EX31_RS11420 0.78 +0.5 218,029 - EX31_RS11420 0.78 +1.1 218,029 - EX31_RS11420 0.78 -2.5 218,029 - EX31_RS11420 0.78 -0.3 218,084 + EX31_RS11420 0.88 -1.2 218,118 - -1.1 218,150 + -0.7 218,151 - -0.4 218,159 + -0.8 218,160 - -2.3 218,160 - -0.6 218,160 - -1.3 218,160 - +0.4 218,160 - +0.6 218,160 - -1.3 218,160 - -1.6 218,160 - -1.3 218,160 - -1.4 218,160 - -1.4 218,160 - -1.6 218,160 - -1.6 218,160 - +0.3 218,160 - -0.4 218,160 - -2.5 218,160 - -1.1 218,160 - -2.0 218,160 - +0.3 218,160 - -0.3 218,160 - +0.5 218,164 - -0.4 218,164 - -0.1 218,164 - -0.4 218,164 - -1.2 218,252 + EX31_RS11425 0.26 -0.8 218,252 + EX31_RS11425 0.26 +1.2 218,252 + EX31_RS11425 0.26 -1.1 218,253 - EX31_RS11425 0.26 -1.6 218,343 - EX31_RS11425 0.52 -2.9
Or see this region's nucleotide sequence