Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS11270 and EX31_RS11275 are separated by 140 nucleotides EX31_RS11275 and EX31_RS11280 overlap by 1 nucleotides
EX31_RS11270: EX31_RS11270 - flagellar hook-associated protein FlgK, at 186,399 to 188,051
_RS11270
EX31_RS11275: EX31_RS11275 - flagellar assembly peptidoglycan hydrolase FlgJ, at 188,192 to 189,142
_RS11275
EX31_RS11280: EX31_RS11280 - flagellar basal body P-ring protein FlgI, at 189,142 to 190,248
_RS11280
Position (kb) 188
189
190 Strain fitness (log2 ratio) -2
-1
0
1 at 187.374 kb on - strand, within EX31_RS11270 at 187.374 kb on - strand, within EX31_RS11270 at 187.374 kb on - strand, within EX31_RS11270 at 187.374 kb on - strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.378 kb on + strand, within EX31_RS11270 at 187.379 kb on - strand, within EX31_RS11270 at 187.379 kb on - strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.382 kb on + strand, within EX31_RS11270 at 187.467 kb on - strand, within EX31_RS11270 at 187.467 kb on - strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.534 kb on + strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.535 kb on - strand, within EX31_RS11270 at 187.600 kb on + strand, within EX31_RS11270 at 187.601 kb on - strand, within EX31_RS11270 at 187.850 kb on - strand, within EX31_RS11270 at 187.890 kb on - strand at 187.892 kb on - strand at 187.905 kb on - strand at 187.917 kb on - strand at 187.926 kb on - strand at 187.947 kb on - strand at 187.949 kb on - strand at 188.033 kb on + strand at 188.034 kb on - strand at 188.037 kb on + strand at 188.038 kb on - strand at 188.038 kb on - strand at 188.038 kb on - strand at 188.082 kb on - strand at 188.091 kb on + strand at 188.092 kb on - strand at 188.092 kb on - strand at 188.095 kb on + strand at 188.095 kb on + strand at 188.200 kb on + strand at 188.200 kb on + strand at 188.220 kb on + strand at 188.286 kb on + strand at 188.287 kb on - strand, within EX31_RS11275 at 188.344 kb on + strand, within EX31_RS11275 at 188.344 kb on + strand, within EX31_RS11275 at 188.384 kb on - strand, within EX31_RS11275 at 188.397 kb on + strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.398 kb on - strand, within EX31_RS11275 at 188.418 kb on + strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.419 kb on - strand, within EX31_RS11275 at 188.427 kb on + strand, within EX31_RS11275 at 188.427 kb on + strand, within EX31_RS11275 at 188.470 kb on + strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.471 kb on - strand, within EX31_RS11275 at 188.642 kb on - strand, within EX31_RS11275 at 188.755 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.926 kb on - strand, within EX31_RS11275 at 188.935 kb on + strand, within EX31_RS11275 at 189.092 kb on - strand at 189.109 kb on - strand at 189.214 kb on - strand at 189.274 kb on + strand, within EX31_RS11280 at 189.552 kb on + strand, within EX31_RS11280 at 189.553 kb on - strand, within EX31_RS11280 at 189.669 kb on + strand, within EX31_RS11280 at 189.669 kb on + strand, within EX31_RS11280 at 189.705 kb on + strand, within EX31_RS11280 at 189.745 kb on - strand, within EX31_RS11280 at 189.766 kb on - strand, within EX31_RS11280 at 189.837 kb on - strand, within EX31_RS11280 at 189.903 kb on + strand, within EX31_RS11280 at 190.047 kb on + strand, within EX31_RS11280 at 190.048 kb on - strand, within EX31_RS11280
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 187,374 - EX31_RS11270 0.59 -1.0 187,374 - EX31_RS11270 0.59 -0.1 187,374 - EX31_RS11270 0.59 -1.5 187,374 - EX31_RS11270 0.59 -1.9 187,378 + EX31_RS11270 0.59 -1.1 187,378 + EX31_RS11270 0.59 -0.9 187,378 + EX31_RS11270 0.59 -0.4 187,378 + EX31_RS11270 0.59 -0.7 187,378 + EX31_RS11270 0.59 -0.8 187,378 + EX31_RS11270 0.59 -0.8 187,379 - EX31_RS11270 0.59 -1.4 187,379 - EX31_RS11270 0.59 -0.9 187,382 + EX31_RS11270 0.59 -0.6 187,382 + EX31_RS11270 0.59 +0.4 187,382 + EX31_RS11270 0.59 -0.4 187,382 + EX31_RS11270 0.59 -1.6 187,467 - EX31_RS11270 0.65 +1.6 187,467 - EX31_RS11270 0.65 -1.5 187,534 + EX31_RS11270 0.69 +1.6 187,534 + EX31_RS11270 0.69 +0.6 187,534 + EX31_RS11270 0.69 -0.8 187,534 + EX31_RS11270 0.69 -0.8 187,534 + EX31_RS11270 0.69 -0.1 187,534 + EX31_RS11270 0.69 +0.4 187,535 - EX31_RS11270 0.69 -0.8 187,535 - EX31_RS11270 0.69 -0.7 187,535 - EX31_RS11270 0.69 -1.9 187,535 - EX31_RS11270 0.69 -1.3 187,535 - EX31_RS11270 0.69 +0.9 187,600 + EX31_RS11270 0.73 -1.2 187,601 - EX31_RS11270 0.73 -0.1 187,850 - EX31_RS11270 0.88 -0.1 187,890 - -0.7 187,892 - +0.3 187,905 - +1.2 187,917 - -0.5 187,926 - -0.8 187,947 - -0.5 187,949 - -0.1 188,033 + +0.3 188,034 - -0.1 188,037 + +1.2 188,038 - +0.0 188,038 - -2.1 188,038 - -0.4 188,082 - +0.1 188,091 + -0.1 188,092 - +0.7 188,092 - -0.3 188,095 + -0.2 188,095 + +0.2 188,200 + -0.6 188,200 + +0.4 188,220 + -0.1 188,286 + -0.7 188,287 - EX31_RS11275 0.10 -1.9 188,344 + EX31_RS11275 0.16 +0.2 188,344 + EX31_RS11275 0.16 -1.0 188,384 - EX31_RS11275 0.20 +1.2 188,397 + EX31_RS11275 0.22 -1.1 188,398 - EX31_RS11275 0.22 -1.6 188,398 - EX31_RS11275 0.22 -1.3 188,398 - EX31_RS11275 0.22 -0.1 188,418 + EX31_RS11275 0.24 +0.9 188,419 - EX31_RS11275 0.24 +0.0 188,419 - EX31_RS11275 0.24 -1.4 188,419 - EX31_RS11275 0.24 -2.2 188,427 + EX31_RS11275 0.25 +1.1 188,427 + EX31_RS11275 0.25 +1.2 188,470 + EX31_RS11275 0.29 +0.2 188,471 - EX31_RS11275 0.29 -1.9 188,471 - EX31_RS11275 0.29 -0.8 188,471 - EX31_RS11275 0.29 -1.1 188,642 - EX31_RS11275 0.47 +0.8 188,755 - EX31_RS11275 0.59 -0.9 188,926 - EX31_RS11275 0.77 -1.6 188,926 - EX31_RS11275 0.77 +0.2 188,926 - EX31_RS11275 0.77 -0.4 188,935 + EX31_RS11275 0.78 +1.7 189,092 - -1.1 189,109 - -2.0 189,214 - +0.4 189,274 + EX31_RS11280 0.12 +0.9 189,552 + EX31_RS11280 0.37 -0.8 189,553 - EX31_RS11280 0.37 -1.0 189,669 + EX31_RS11280 0.48 +0.5 189,669 + EX31_RS11280 0.48 +1.2 189,705 + EX31_RS11280 0.51 -2.0 189,745 - EX31_RS11280 0.54 -0.6 189,766 - EX31_RS11280 0.56 -2.0 189,837 - EX31_RS11280 0.63 +0.6 189,903 + EX31_RS11280 0.69 +0.6 190,047 + EX31_RS11280 0.82 -1.0 190,048 - EX31_RS11280 0.82 -0.6
Or see this region's nucleotide sequence