Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10570 and EX31_RS10575 are separated by 122 nucleotides EX31_RS10575 and EX31_RS10580 are separated by 136 nucleotides EX31_RS10580 and EX31_RS10585 are separated by 24 nucleotides EX31_RS10585 and EX31_RS10590 are separated by 3 nucleotides
EX31_RS10570: EX31_RS10570 - lytic murein transglycosylase, at 26,786 to 27,961
_RS10570
EX31_RS10575: EX31_RS10575 - hypothetical protein, at 28,084 to 28,356
_RS10575
EX31_RS10580: EX31_RS10580 - septum site-determining protein MinC, at 28,493 to 29,182
_RS10580
EX31_RS10585: EX31_RS10585 - septum site-determining protein MinD, at 29,207 to 30,019
_RS10585
EX31_RS10590: EX31_RS10590 - cell division topological specificity factor MinE, at 30,023 to 30,292
_RS10590
Position (kb) 28
29
30 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 27.513 kb on + strand, within EX31_RS10570 at 27.513 kb on + strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.514 kb on - strand, within EX31_RS10570 at 27.593 kb on - strand, within EX31_RS10570 at 27.625 kb on - strand, within EX31_RS10570 at 27.625 kb on - strand, within EX31_RS10570 at 27.625 kb on - strand, within EX31_RS10570 at 27.637 kb on + strand, within EX31_RS10570 at 27.638 kb on - strand, within EX31_RS10570 at 27.768 kb on + strand, within EX31_RS10570 at 27.768 kb on + strand, within EX31_RS10570 at 27.769 kb on - strand, within EX31_RS10570 at 27.769 kb on - strand, within EX31_RS10570 at 27.893 kb on - strand at 27.935 kb on + strand at 28.002 kb on + strand at 28.003 kb on - strand at 28.003 kb on - strand at 28.118 kb on - strand, within EX31_RS10575 at 28.149 kb on - strand, within EX31_RS10575 at 28.151 kb on + strand, within EX31_RS10575 at 28.152 kb on - strand, within EX31_RS10575 at 28.182 kb on + strand, within EX31_RS10575 at 28.225 kb on + strand, within EX31_RS10575 at 28.226 kb on - strand, within EX31_RS10575 at 28.226 kb on - strand, within EX31_RS10575 at 28.307 kb on + strand, within EX31_RS10575 at 28.307 kb on + strand, within EX31_RS10575 at 28.307 kb on + strand, within EX31_RS10575 at 28.308 kb on - strand, within EX31_RS10575 at 28.308 kb on - strand, within EX31_RS10575 at 28.314 kb on - strand, within EX31_RS10575 at 28.332 kb on + strand at 28.338 kb on + strand at 28.339 kb on - strand at 28.339 kb on - strand at 28.340 kb on + strand at 28.340 kb on + strand at 28.340 kb on + strand at 28.341 kb on - strand at 28.341 kb on - strand at 28.341 kb on - strand at 28.341 kb on - strand at 28.369 kb on - strand at 28.369 kb on - strand at 28.369 kb on - strand at 28.398 kb on - strand at 28.399 kb on + strand at 28.399 kb on + strand at 28.400 kb on - strand at 28.405 kb on + strand at 28.405 kb on + strand at 28.409 kb on + strand at 28.410 kb on - strand at 28.411 kb on + strand at 28.425 kb on + strand at 28.425 kb on + strand at 28.425 kb on + strand at 28.425 kb on + strand at 28.425 kb on + strand at 28.425 kb on + strand at 28.425 kb on + strand at 28.465 kb on + strand at 28.465 kb on + strand at 28.490 kb on + strand at 28.490 kb on + strand at 28.531 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.551 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.555 kb on + strand at 28.573 kb on + strand, within EX31_RS10580 at 28.855 kb on + strand, within EX31_RS10580 at 28.982 kb on + strand, within EX31_RS10580 at 29.067 kb on + strand, within EX31_RS10580 at 29.067 kb on + strand, within EX31_RS10580 at 29.067 kb on + strand, within EX31_RS10580 at 29.067 kb on + strand, within EX31_RS10580 at 29.067 kb on + strand, within EX31_RS10580 at 29.148 kb on + strand at 29.148 kb on + strand at 29.152 kb on + strand at 29.152 kb on + strand at 29.166 kb on + strand at 29.166 kb on + strand at 29.166 kb on + strand at 29.166 kb on + strand at 29.166 kb on + strand at 29.180 kb on + strand at 29.220 kb on + strand at 29.220 kb on + strand at 29.220 kb on + strand at 29.227 kb on + strand at 29.227 kb on + strand at 29.227 kb on + strand at 29.227 kb on + strand at 29.286 kb on + strand at 29.310 kb on + strand, within EX31_RS10585 at 29.310 kb on + strand, within EX31_RS10585 at 29.310 kb on + strand, within EX31_RS10585 at 29.310 kb on + strand, within EX31_RS10585 at 29.310 kb on + strand, within EX31_RS10585 at 29.314 kb on + strand, within EX31_RS10585 at 29.458 kb on + strand, within EX31_RS10585 at 29.753 kb on + strand, within EX31_RS10585 at 29.921 kb on + strand, within EX31_RS10585 at 29.921 kb on + strand, within EX31_RS10585 at 29.929 kb on + strand, within EX31_RS10585
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 27,513 + EX31_RS10570 0.62 +0.1 27,513 + EX31_RS10570 0.62 -0.7 27,514 - EX31_RS10570 0.62 +1.6 27,514 - EX31_RS10570 0.62 +0.6 27,514 - EX31_RS10570 0.62 +0.0 27,514 - EX31_RS10570 0.62 +1.6 27,593 - EX31_RS10570 0.69 -1.1 27,625 - EX31_RS10570 0.71 +0.1 27,625 - EX31_RS10570 0.71 +0.0 27,625 - EX31_RS10570 0.71 -1.7 27,637 + EX31_RS10570 0.72 +1.4 27,638 - EX31_RS10570 0.72 +0.5 27,768 + EX31_RS10570 0.84 +0.1 27,768 + EX31_RS10570 0.84 -0.1 27,769 - EX31_RS10570 0.84 +0.7 27,769 - EX31_RS10570 0.84 +0.4 27,893 - +0.6 27,935 + +0.4 28,002 + +0.2 28,003 - +0.4 28,003 - -1.8 28,118 - EX31_RS10575 0.12 -1.6 28,149 - EX31_RS10575 0.24 -0.4 28,151 + EX31_RS10575 0.25 +1.0 28,152 - EX31_RS10575 0.25 +0.2 28,182 + EX31_RS10575 0.36 -0.1 28,225 + EX31_RS10575 0.52 +0.5 28,226 - EX31_RS10575 0.52 +1.5 28,226 - EX31_RS10575 0.52 +0.0 28,307 + EX31_RS10575 0.82 +0.7 28,307 + EX31_RS10575 0.82 +1.8 28,307 + EX31_RS10575 0.82 +0.4 28,308 - EX31_RS10575 0.82 +1.5 28,308 - EX31_RS10575 0.82 +2.2 28,314 - EX31_RS10575 0.84 +1.2 28,332 + +0.2 28,338 + +0.3 28,339 - +0.9 28,339 - -1.6 28,340 + +1.7 28,340 + +1.2 28,340 + -0.8 28,341 - +0.2 28,341 - +0.6 28,341 - +0.0 28,341 - +0.4 28,369 - -0.2 28,369 - +0.2 28,369 - +1.8 28,398 - +1.6 28,399 + -0.4 28,399 + +1.4 28,400 - +1.6 28,405 + +1.2 28,405 + +0.2 28,409 + +0.8 28,410 - +1.2 28,411 + -0.8 28,425 + -0.1 28,425 + +0.4 28,425 + +0.2 28,425 + -0.6 28,425 + -0.4 28,425 + -1.0 28,425 + +0.2 28,465 + -1.6 28,465 + -1.7 28,490 + +0.1 28,490 + +0.0 28,531 + -0.8 28,551 + -0.5 28,551 + +0.2 28,551 + -0.3 28,551 + -3.4 28,551 + -1.0 28,551 + -0.1 28,551 + +0.1 28,551 + -1.7 28,551 + +1.4 28,555 + -0.1 28,555 + +0.7 28,555 + +0.2 28,555 + -0.3 28,555 + +1.2 28,555 + -2.3 28,555 + -0.6 28,555 + +0.9 28,555 + -0.6 28,555 + -0.0 28,573 + EX31_RS10580 0.12 -1.0 28,855 + EX31_RS10580 0.52 -0.3 28,982 + EX31_RS10580 0.71 -2.5 29,067 + EX31_RS10580 0.83 -0.6 29,067 + EX31_RS10580 0.83 -0.9 29,067 + EX31_RS10580 0.83 -3.0 29,067 + EX31_RS10580 0.83 -1.3 29,067 + EX31_RS10580 0.83 -0.6 29,148 + -3.0 29,148 + -0.8 29,152 + -1.0 29,152 + -0.7 29,166 + +0.9 29,166 + -0.6 29,166 + +0.9 29,166 + +0.5 29,166 + +0.3 29,180 + -1.4 29,220 + +1.5 29,220 + +0.1 29,220 + +0.9 29,227 + +0.9 29,227 + -1.7 29,227 + -0.4 29,227 + +1.3 29,286 + -2.0 29,310 + EX31_RS10585 0.13 +0.4 29,310 + EX31_RS10585 0.13 +0.4 29,310 + EX31_RS10585 0.13 +0.5 29,310 + EX31_RS10585 0.13 +0.5 29,310 + EX31_RS10585 0.13 +0.0 29,314 + EX31_RS10585 0.13 +0.9 29,458 + EX31_RS10585 0.31 -1.9 29,753 + EX31_RS10585 0.67 -1.6 29,921 + EX31_RS10585 0.88 -0.7 29,921 + EX31_RS10585 0.88 +0.2 29,929 + EX31_RS10585 0.89 +1.3
Or see this region's nucleotide sequence