Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10530 and EX31_RS10535 are separated by 744 nucleotides EX31_RS10535 and EX31_RS10540 overlap by 1 nucleotides EX31_RS10540 and EX31_RS10545 are separated by 1 nucleotides
EX31_RS10530: EX31_RS10530 - anti-adapter protein IraP, at 20,394 to 20,735
_RS10530
EX31_RS10535: EX31_RS10535 - GtrA family protein, at 21,480 to 21,839
_RS10535
EX31_RS10540: EX31_RS10540 - glycosyltransferase family 2 protein, at 21,839 to 22,756
_RS10540
EX31_RS10545: EX31_RS10545 - hypothetical protein, at 22,758 to 24,011
_RS10545
Position (kb)
21
22 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 20.498 kb on + strand, within EX31_RS10530 at 20.598 kb on - strand, within EX31_RS10530 at 20.598 kb on - strand, within EX31_RS10530 at 20.598 kb on - strand, within EX31_RS10530 at 20.598 kb on - strand, within EX31_RS10530 at 20.609 kb on + strand, within EX31_RS10530 at 20.733 kb on + strand at 20.738 kb on + strand at 20.738 kb on + strand at 20.751 kb on + strand at 20.831 kb on - strand at 20.831 kb on - strand at 20.880 kb on - strand at 20.899 kb on + strand at 20.934 kb on + strand at 21.127 kb on + strand at 21.183 kb on + strand at 21.183 kb on + strand at 21.184 kb on - strand at 21.184 kb on - strand at 21.223 kb on - strand at 21.225 kb on - strand at 21.225 kb on - strand at 21.225 kb on - strand at 21.225 kb on - strand at 21.225 kb on - strand at 21.225 kb on - strand at 21.253 kb on + strand at 21.254 kb on - strand at 21.256 kb on - strand at 21.256 kb on - strand at 21.256 kb on - strand at 21.256 kb on - strand at 21.256 kb on - strand at 21.283 kb on - strand at 21.320 kb on + strand at 21.320 kb on + strand at 21.321 kb on - strand at 21.321 kb on - strand at 21.321 kb on - strand at 21.321 kb on - strand at 21.321 kb on - strand at 21.397 kb on - strand at 21.430 kb on + strand at 21.431 kb on - strand at 21.431 kb on - strand at 21.473 kb on + strand at 21.475 kb on + strand at 21.475 kb on + strand at 21.510 kb on - strand at 21.556 kb on + strand, within EX31_RS10535 at 21.666 kb on - strand, within EX31_RS10535 at 21.784 kb on + strand, within EX31_RS10535 at 21.855 kb on + strand at 21.970 kb on + strand, within EX31_RS10540 at 22.182 kb on + strand, within EX31_RS10540 at 22.198 kb on + strand, within EX31_RS10540 at 22.199 kb on - strand, within EX31_RS10540 at 22.255 kb on + strand, within EX31_RS10540 at 22.255 kb on + strand, within EX31_RS10540 at 22.255 kb on + strand, within EX31_RS10540 at 22.263 kb on + strand, within EX31_RS10540 at 22.263 kb on + strand, within EX31_RS10540 at 22.263 kb on + strand, within EX31_RS10540 at 22.263 kb on + strand, within EX31_RS10540 at 22.330 kb on + strand, within EX31_RS10540 at 22.333 kb on + strand, within EX31_RS10540 at 22.333 kb on + strand, within EX31_RS10540 at 22.410 kb on - strand, within EX31_RS10540 at 22.411 kb on + strand, within EX31_RS10540 at 22.411 kb on + strand, within EX31_RS10540 at 22.411 kb on + strand, within EX31_RS10540 at 22.510 kb on + strand, within EX31_RS10540 at 22.523 kb on + strand, within EX31_RS10540 at 22.523 kb on + strand, within EX31_RS10540 at 22.523 kb on + strand, within EX31_RS10540 at 22.523 kb on + strand, within EX31_RS10540 at 22.524 kb on - strand, within EX31_RS10540 at 22.524 kb on - strand, within EX31_RS10540 at 22.548 kb on + strand, within EX31_RS10540 at 22.578 kb on + strand, within EX31_RS10540 at 22.578 kb on + strand, within EX31_RS10540 at 22.579 kb on - strand, within EX31_RS10540 at 22.582 kb on + strand, within EX31_RS10540 at 22.588 kb on + strand, within EX31_RS10540 at 22.588 kb on + strand, within EX31_RS10540 at 22.588 kb on + strand, within EX31_RS10540 at 22.588 kb on + strand, within EX31_RS10540 at 22.588 kb on + strand, within EX31_RS10540 at 22.589 kb on - strand, within EX31_RS10540 at 22.589 kb on - strand, within EX31_RS10540 at 22.590 kb on + strand, within EX31_RS10540 at 22.621 kb on + strand, within EX31_RS10540 at 22.651 kb on + strand, within EX31_RS10540 at 22.687 kb on + strand at 22.690 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 20,498 + EX31_RS10530 0.30 +1.0 20,598 - EX31_RS10530 0.60 +0.8 20,598 - EX31_RS10530 0.60 -0.8 20,598 - EX31_RS10530 0.60 +0.7 20,598 - EX31_RS10530 0.60 +0.9 20,609 + EX31_RS10530 0.63 +2.2 20,733 + -1.6 20,738 + +0.3 20,738 + -0.5 20,751 + +1.3 20,831 - +0.5 20,831 - -0.2 20,880 - +0.0 20,899 + +0.2 20,934 + +1.2 21,127 + -1.0 21,183 + +0.6 21,183 + +0.8 21,184 - +0.2 21,184 - -0.4 21,223 - -0.3 21,225 - +0.2 21,225 - +0.6 21,225 - -0.9 21,225 - +0.1 21,225 - -0.5 21,225 - +0.6 21,253 + +0.5 21,254 - -0.5 21,256 - +0.2 21,256 - -0.7 21,256 - +0.6 21,256 - +0.7 21,256 - -1.1 21,283 - -0.6 21,320 + +0.2 21,320 + -0.5 21,321 - +1.0 21,321 - -1.0 21,321 - +0.9 21,321 - +0.5 21,321 - -0.6 21,397 - +1.0 21,430 + +1.4 21,431 - +2.2 21,431 - -0.3 21,473 + -0.3 21,475 + +0.1 21,475 + -1.3 21,510 - +0.2 21,556 + EX31_RS10535 0.21 -0.4 21,666 - EX31_RS10535 0.52 -0.1 21,784 + EX31_RS10535 0.84 +0.3 21,855 + +0.7 21,970 + EX31_RS10540 0.14 +1.4 22,182 + EX31_RS10540 0.37 +0.6 22,198 + EX31_RS10540 0.39 +1.6 22,199 - EX31_RS10540 0.39 +0.6 22,255 + EX31_RS10540 0.45 +1.7 22,255 + EX31_RS10540 0.45 +0.8 22,255 + EX31_RS10540 0.45 -0.2 22,263 + EX31_RS10540 0.46 +0.9 22,263 + EX31_RS10540 0.46 +0.7 22,263 + EX31_RS10540 0.46 +1.7 22,263 + EX31_RS10540 0.46 -0.8 22,330 + EX31_RS10540 0.53 -0.4 22,333 + EX31_RS10540 0.54 +0.7 22,333 + EX31_RS10540 0.54 +0.2 22,410 - EX31_RS10540 0.62 +0.2 22,411 + EX31_RS10540 0.62 +2.2 22,411 + EX31_RS10540 0.62 -0.1 22,411 + EX31_RS10540 0.62 +1.8 22,510 + EX31_RS10540 0.73 -0.4 22,523 + EX31_RS10540 0.75 +0.6 22,523 + EX31_RS10540 0.75 +1.0 22,523 + EX31_RS10540 0.75 +0.4 22,523 + EX31_RS10540 0.75 +0.6 22,524 - EX31_RS10540 0.75 +1.2 22,524 - EX31_RS10540 0.75 -1.0 22,548 + EX31_RS10540 0.77 -1.3 22,578 + EX31_RS10540 0.81 +2.0 22,578 + EX31_RS10540 0.81 -0.0 22,579 - EX31_RS10540 0.81 +0.3 22,582 + EX31_RS10540 0.81 +0.4 22,588 + EX31_RS10540 0.82 +1.4 22,588 + EX31_RS10540 0.82 +1.4 22,588 + EX31_RS10540 0.82 -1.9 22,588 + EX31_RS10540 0.82 +0.8 22,588 + EX31_RS10540 0.82 +0.1 22,589 - EX31_RS10540 0.82 +1.2 22,589 - EX31_RS10540 0.82 +2.2 22,590 + EX31_RS10540 0.82 +2.2 22,621 + EX31_RS10540 0.85 -0.3 22,651 + EX31_RS10540 0.88 +0.2 22,687 + -1.0 22,690 - -0.3
Or see this region's nucleotide sequence