Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS08530 and EX31_RS08535 overlap by 1 nucleotides EX31_RS08535 and EX31_RS08540 are separated by 79 nucleotides
EX31_RS08530: EX31_RS08530 - tRNA pseudouridine(55) synthase TruB, at 212,948 to 213,892
_RS08530
EX31_RS08535: EX31_RS08535 - 30S ribosome-binding factor RbfA, at 213,892 to 214,305
_RS08535
EX31_RS08540: EX31_RS08540 - translation initiation factor IF-2, at 214,385 to 217,078
_RS08540
Position (kb) 213
214
215 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 212.893 kb on - strand at 212.958 kb on + strand at 212.959 kb on - strand at 212.959 kb on - strand at 212.959 kb on - strand at 212.959 kb on - strand at 213.025 kb on + strand at 213.025 kb on + strand at 213.025 kb on + strand at 213.025 kb on + strand at 213.025 kb on + strand at 213.025 kb on + strand at 213.026 kb on - strand at 213.026 kb on - strand at 213.026 kb on - strand at 213.026 kb on - strand at 213.026 kb on - strand at 213.026 kb on - strand at 213.026 kb on - strand at 213.026 kb on - strand at 213.106 kb on - strand, within EX31_RS08530 at 213.106 kb on - strand, within EX31_RS08530 at 213.106 kb on - strand, within EX31_RS08530 at 213.270 kb on + strand, within EX31_RS08530 at 213.270 kb on + strand, within EX31_RS08530 at 213.271 kb on - strand, within EX31_RS08530 at 213.298 kb on - strand, within EX31_RS08530 at 213.298 kb on - strand, within EX31_RS08530 at 213.298 kb on - strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.357 kb on + strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.358 kb on - strand, within EX31_RS08530 at 213.410 kb on + strand, within EX31_RS08530 at 213.410 kb on + strand, within EX31_RS08530 at 213.410 kb on + strand, within EX31_RS08530 at 213.410 kb on + strand, within EX31_RS08530 at 213.411 kb on - strand, within EX31_RS08530 at 213.413 kb on - strand, within EX31_RS08530 at 213.413 kb on - strand, within EX31_RS08530 at 213.426 kb on + strand, within EX31_RS08530 at 213.426 kb on + strand, within EX31_RS08530 at 213.427 kb on - strand, within EX31_RS08530 at 213.427 kb on - strand, within EX31_RS08530 at 213.427 kb on - strand, within EX31_RS08530 at 213.427 kb on - strand, within EX31_RS08530 at 213.477 kb on + strand, within EX31_RS08530 at 213.484 kb on - strand, within EX31_RS08530 at 213.501 kb on + strand, within EX31_RS08530 at 213.501 kb on + strand, within EX31_RS08530 at 213.501 kb on + strand, within EX31_RS08530 at 213.502 kb on - strand, within EX31_RS08530 at 213.516 kb on + strand, within EX31_RS08530 at 213.516 kb on + strand, within EX31_RS08530 at 213.516 kb on + strand, within EX31_RS08530 at 213.516 kb on + strand, within EX31_RS08530 at 213.516 kb on + strand, within EX31_RS08530 at 213.516 kb on + strand, within EX31_RS08530 at 213.516 kb on + strand, within EX31_RS08530 at 213.517 kb on - strand, within EX31_RS08530 at 213.517 kb on - strand, within EX31_RS08530 at 213.517 kb on - strand, within EX31_RS08530 at 213.517 kb on - strand, within EX31_RS08530 at 213.517 kb on - strand, within EX31_RS08530 at 213.631 kb on + strand, within EX31_RS08530 at 213.631 kb on + strand, within EX31_RS08530 at 213.637 kb on + strand, within EX31_RS08530 at 213.637 kb on + strand, within EX31_RS08530 at 213.637 kb on + strand, within EX31_RS08530 at 213.638 kb on - strand, within EX31_RS08530 at 213.786 kb on + strand, within EX31_RS08530 at 213.786 kb on + strand, within EX31_RS08530 at 213.786 kb on + strand, within EX31_RS08530 at 213.787 kb on - strand, within EX31_RS08530 at 213.787 kb on - strand, within EX31_RS08530 at 213.787 kb on - strand, within EX31_RS08530 at 213.817 kb on + strand at 213.817 kb on + strand at 213.818 kb on - strand at 213.818 kb on - strand at 213.818 kb on - strand at 213.846 kb on - strand at 213.860 kb on + strand at 213.860 kb on + strand at 213.860 kb on + strand at 213.860 kb on + strand at 213.860 kb on + strand at 213.860 kb on + strand at 213.860 kb on + strand at 213.860 kb on + strand at 213.861 kb on - strand at 213.861 kb on - strand at 213.861 kb on - strand at 213.861 kb on - strand at 213.861 kb on - strand at 213.861 kb on - strand at 213.861 kb on - strand at 213.893 kb on + strand at 213.893 kb on + strand at 213.893 kb on + strand at 213.893 kb on + strand at 213.894 kb on - strand at 213.894 kb on - strand at 213.970 kb on - strand, within EX31_RS08535 at 213.970 kb on - strand, within EX31_RS08535 at 213.970 kb on - strand, within EX31_RS08535 at 213.970 kb on - strand, within EX31_RS08535 at 213.976 kb on - strand, within EX31_RS08535 at 213.976 kb on - strand, within EX31_RS08535 at 213.976 kb on - strand, within EX31_RS08535 at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.334 kb on - strand at 214.338 kb on - strand at 214.338 kb on - strand at 214.338 kb on - strand at 214.338 kb on - strand at 214.373 kb on - strand at 214.373 kb on - strand at 214.373 kb on - strand at 214.373 kb on - strand at 214.394 kb on - strand at 214.394 kb on - strand at 214.394 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand at 214.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 212,893 - -1.3 212,958 + +0.9 212,959 - +0.2 212,959 - -0.7 212,959 - +0.5 212,959 - -0.2 213,025 + +0.5 213,025 + +0.7 213,025 + -0.2 213,025 + +0.4 213,025 + -0.1 213,025 + -0.5 213,026 - -0.6 213,026 - -1.6 213,026 - -0.6 213,026 - +0.1 213,026 - +0.2 213,026 - +0.1 213,026 - -0.3 213,026 - +0.3 213,106 - EX31_RS08530 0.17 +0.6 213,106 - EX31_RS08530 0.17 +1.4 213,106 - EX31_RS08530 0.17 -0.5 213,270 + EX31_RS08530 0.34 +1.6 213,270 + EX31_RS08530 0.34 -2.1 213,271 - EX31_RS08530 0.34 -0.3 213,298 - EX31_RS08530 0.37 -0.4 213,298 - EX31_RS08530 0.37 -0.2 213,298 - EX31_RS08530 0.37 +0.4 213,357 + EX31_RS08530 0.43 -0.4 213,357 + EX31_RS08530 0.43 +1.5 213,357 + EX31_RS08530 0.43 +0.8 213,357 + EX31_RS08530 0.43 +0.7 213,357 + EX31_RS08530 0.43 -0.5 213,357 + EX31_RS08530 0.43 -1.6 213,357 + EX31_RS08530 0.43 +0.9 213,357 + EX31_RS08530 0.43 +0.9 213,357 + EX31_RS08530 0.43 -0.2 213,357 + EX31_RS08530 0.43 +2.7 213,357 + EX31_RS08530 0.43 -1.0 213,358 - EX31_RS08530 0.43 -0.4 213,358 - EX31_RS08530 0.43 -0.0 213,358 - EX31_RS08530 0.43 -1.2 213,358 - EX31_RS08530 0.43 -0.4 213,358 - EX31_RS08530 0.43 +0.9 213,358 - EX31_RS08530 0.43 +0.2 213,358 - EX31_RS08530 0.43 -0.7 213,358 - EX31_RS08530 0.43 -0.0 213,358 - EX31_RS08530 0.43 -1.0 213,358 - EX31_RS08530 0.43 +0.8 213,358 - EX31_RS08530 0.43 -0.7 213,358 - EX31_RS08530 0.43 -0.3 213,358 - EX31_RS08530 0.43 +1.1 213,358 - EX31_RS08530 0.43 -3.0 213,358 - EX31_RS08530 0.43 -0.8 213,358 - EX31_RS08530 0.43 +0.6 213,358 - EX31_RS08530 0.43 +0.7 213,410 + EX31_RS08530 0.49 -0.8 213,410 + EX31_RS08530 0.49 -2.1 213,410 + EX31_RS08530 0.49 -0.8 213,410 + EX31_RS08530 0.49 +0.1 213,411 - EX31_RS08530 0.49 -0.4 213,413 - EX31_RS08530 0.49 -1.0 213,413 - EX31_RS08530 0.49 +0.8 213,426 + EX31_RS08530 0.51 -0.5 213,426 + EX31_RS08530 0.51 -0.3 213,427 - EX31_RS08530 0.51 +0.3 213,427 - EX31_RS08530 0.51 -0.4 213,427 - EX31_RS08530 0.51 -0.3 213,427 - EX31_RS08530 0.51 +0.0 213,477 + EX31_RS08530 0.56 -0.1 213,484 - EX31_RS08530 0.57 +0.2 213,501 + EX31_RS08530 0.59 -1.3 213,501 + EX31_RS08530 0.59 +0.3 213,501 + EX31_RS08530 0.59 +1.4 213,502 - EX31_RS08530 0.59 -1.2 213,516 + EX31_RS08530 0.60 -0.8 213,516 + EX31_RS08530 0.60 -0.9 213,516 + EX31_RS08530 0.60 -0.8 213,516 + EX31_RS08530 0.60 -1.1 213,516 + EX31_RS08530 0.60 -0.1 213,516 + EX31_RS08530 0.60 -0.2 213,516 + EX31_RS08530 0.60 -0.0 213,517 - EX31_RS08530 0.60 +0.7 213,517 - EX31_RS08530 0.60 -0.2 213,517 - EX31_RS08530 0.60 -0.3 213,517 - EX31_RS08530 0.60 -0.8 213,517 - EX31_RS08530 0.60 -0.2 213,631 + EX31_RS08530 0.72 +0.7 213,631 + EX31_RS08530 0.72 -0.3 213,637 + EX31_RS08530 0.73 -0.2 213,637 + EX31_RS08530 0.73 -0.6 213,637 + EX31_RS08530 0.73 -0.4 213,638 - EX31_RS08530 0.73 -1.0 213,786 + EX31_RS08530 0.89 +0.3 213,786 + EX31_RS08530 0.89 -1.2 213,786 + EX31_RS08530 0.89 +0.3 213,787 - EX31_RS08530 0.89 +0.0 213,787 - EX31_RS08530 0.89 -0.5 213,787 - EX31_RS08530 0.89 +0.1 213,817 + +0.6 213,817 + +0.1 213,818 - -1.0 213,818 - +0.2 213,818 - -0.4 213,846 - +2.5 213,860 + -0.8 213,860 + +0.1 213,860 + -1.2 213,860 + -0.1 213,860 + +1.5 213,860 + +1.9 213,860 + -0.5 213,860 + -0.4 213,861 - +0.1 213,861 - -0.3 213,861 - -1.2 213,861 - -0.3 213,861 - -1.4 213,861 - -1.1 213,861 - +0.4 213,893 + +1.4 213,893 + +0.7 213,893 + -0.0 213,893 + +0.6 213,894 - +0.1 213,894 - +0.1 213,970 - EX31_RS08535 0.19 -2.6 213,970 - EX31_RS08535 0.19 -2.4 213,970 - EX31_RS08535 0.19 -1.3 213,970 - EX31_RS08535 0.19 -1.3 213,976 - EX31_RS08535 0.20 -0.7 213,976 - EX31_RS08535 0.20 -0.9 213,976 - EX31_RS08535 0.20 -0.3 214,334 - -2.9 214,334 - +0.6 214,334 - -0.3 214,334 - +0.5 214,334 - -0.2 214,338 - +0.3 214,338 - +0.9 214,338 - -0.3 214,338 - +0.2 214,373 - +1.0 214,373 - -0.8 214,373 - +0.8 214,373 - -0.5 214,394 - -1.1 214,394 - -1.3 214,394 - -2.0 214,397 - -0.2 214,397 - -0.7 214,397 - +0.2 214,397 - -0.1 214,397 - +1.5 214,397 - -1.8 214,397 - -2.0 214,397 - -0.7 214,397 - -0.2
Or see this region's nucleotide sequence