Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07855 and EX31_RS07860 are separated by 2 nucleotides EX31_RS07860 and EX31_RS07865 are separated by 167 nucleotides EX31_RS07865 and EX31_RS07870 are separated by 59 nucleotides EX31_RS07870 and EX31_RS07875 are separated by 1 nucleotides
EX31_RS07855: EX31_RS07855 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 53,749 to 54,468
_RS07855
EX31_RS07860: EX31_RS07860 - cell division protein FtsB, at 54,471 to 54,791
_RS07860
EX31_RS07865: EX31_RS07865 - DUF3561 family protein, at 54,959 to 55,339
_RS07865
EX31_RS07870: EX31_RS07870 - adenylyl-sulfate kinase, at 55,399 to 56,022
_RS07870
EX31_RS07875: EX31_RS07875 - sulfate adenylyltransferase subunit CysN, at 56,024 to 57,451
_RS07875
Position (kb) 55
56
57 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 54.781 kb on - strand at 54.781 kb on - strand at 54.859 kb on - strand at 54.880 kb on + strand at 54.880 kb on + strand at 54.880 kb on + strand at 54.881 kb on - strand at 54.881 kb on - strand at 54.881 kb on - strand at 54.892 kb on + strand at 54.892 kb on + strand at 54.893 kb on - strand at 54.917 kb on + strand at 54.918 kb on - strand at 55.034 kb on - strand, within EX31_RS07865 at 55.073 kb on + strand, within EX31_RS07865 at 55.073 kb on + strand, within EX31_RS07865 at 55.074 kb on - strand, within EX31_RS07865 at 55.074 kb on - strand, within EX31_RS07865 at 55.074 kb on - strand, within EX31_RS07865 at 55.119 kb on + strand, within EX31_RS07865 at 55.119 kb on + strand, within EX31_RS07865 at 55.119 kb on + strand, within EX31_RS07865 at 55.120 kb on - strand, within EX31_RS07865 at 55.120 kb on - strand, within EX31_RS07865 at 55.150 kb on - strand, within EX31_RS07865 at 55.182 kb on + strand, within EX31_RS07865 at 55.182 kb on + strand, within EX31_RS07865 at 55.183 kb on - strand, within EX31_RS07865 at 55.218 kb on + strand, within EX31_RS07865 at 55.218 kb on + strand, within EX31_RS07865 at 55.218 kb on + strand, within EX31_RS07865 at 55.218 kb on + strand, within EX31_RS07865 at 55.218 kb on + strand, within EX31_RS07865 at 55.218 kb on + strand, within EX31_RS07865 at 55.328 kb on + strand at 55.329 kb on - strand at 55.339 kb on + strand at 55.339 kb on + strand at 55.339 kb on + strand at 55.339 kb on + strand at 55.339 kb on + strand at 55.339 kb on + strand at 55.339 kb on + strand at 55.339 kb on + strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.340 kb on - strand at 55.352 kb on + strand at 55.353 kb on - strand at 55.410 kb on + strand at 55.410 kb on + strand at 55.410 kb on + strand at 55.411 kb on - strand at 55.411 kb on - strand at 55.411 kb on - strand at 55.411 kb on - strand at 55.436 kb on - strand at 55.439 kb on - strand at 55.623 kb on + strand, within EX31_RS07870 at 55.623 kb on + strand, within EX31_RS07870 at 55.623 kb on + strand, within EX31_RS07870 at 55.623 kb on + strand, within EX31_RS07870 at 55.624 kb on - strand, within EX31_RS07870 at 55.624 kb on - strand, within EX31_RS07870 at 55.624 kb on - strand, within EX31_RS07870 at 55.624 kb on - strand, within EX31_RS07870 at 55.738 kb on + strand, within EX31_RS07870 at 55.739 kb on - strand, within EX31_RS07870 at 55.739 kb on - strand, within EX31_RS07870 at 55.810 kb on - strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.826 kb on + strand, within EX31_RS07870 at 55.827 kb on - strand, within EX31_RS07870 at 55.827 kb on - strand, within EX31_RS07870 at 55.827 kb on - strand, within EX31_RS07870 at 55.847 kb on + strand, within EX31_RS07870 at 55.847 kb on + strand, within EX31_RS07870 at 55.847 kb on + strand, within EX31_RS07870 at 55.996 kb on + strand at 56.025 kb on + strand at 56.025 kb on + strand at 56.025 kb on + strand at 56.025 kb on + strand at 56.025 kb on + strand at 56.026 kb on - strand at 56.109 kb on + strand at 56.109 kb on + strand at 56.109 kb on + strand at 56.109 kb on + strand at 56.109 kb on + strand at 56.109 kb on + strand at 56.110 kb on - strand at 56.110 kb on - strand at 56.110 kb on - strand at 56.110 kb on - strand at 56.110 kb on - strand at 56.114 kb on - strand at 56.128 kb on - strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.129 kb on + strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.130 kb on - strand at 56.171 kb on - strand, within EX31_RS07875 at 56.171 kb on - strand, within EX31_RS07875 at 56.226 kb on + strand, within EX31_RS07875 at 56.226 kb on + strand, within EX31_RS07875 at 56.226 kb on + strand, within EX31_RS07875 at 56.226 kb on + strand, within EX31_RS07875 at 56.227 kb on - strand, within EX31_RS07875 at 56.227 kb on - strand, within EX31_RS07875 at 56.227 kb on - strand, within EX31_RS07875 at 56.230 kb on + strand, within EX31_RS07875 at 56.230 kb on + strand, within EX31_RS07875 at 56.230 kb on + strand, within EX31_RS07875 at 56.321 kb on - strand, within EX31_RS07875 at 56.321 kb on - strand, within EX31_RS07875 at 56.334 kb on + strand, within EX31_RS07875 at 56.335 kb on - strand, within EX31_RS07875 at 56.335 kb on - strand, within EX31_RS07875 at 56.342 kb on - strand, within EX31_RS07875 at 56.388 kb on + strand, within EX31_RS07875 at 56.389 kb on - strand, within EX31_RS07875 at 56.389 kb on - strand, within EX31_RS07875 at 56.501 kb on - strand, within EX31_RS07875 at 56.590 kb on + strand, within EX31_RS07875 at 56.590 kb on + strand, within EX31_RS07875 at 56.591 kb on - strand, within EX31_RS07875 at 56.591 kb on - strand, within EX31_RS07875 at 56.591 kb on - strand, within EX31_RS07875 at 56.591 kb on - strand, within EX31_RS07875 at 56.591 kb on - strand, within EX31_RS07875 at 56.612 kb on + strand, within EX31_RS07875 at 56.612 kb on + strand, within EX31_RS07875 at 56.613 kb on - strand, within EX31_RS07875 at 56.613 kb on - strand, within EX31_RS07875 at 56.613 kb on - strand, within EX31_RS07875 at 56.613 kb on - strand, within EX31_RS07875 at 56.664 kb on + strand, within EX31_RS07875 at 56.665 kb on - strand, within EX31_RS07875 at 56.697 kb on + strand, within EX31_RS07875 at 56.697 kb on + strand, within EX31_RS07875 at 56.698 kb on - strand, within EX31_RS07875 at 56.698 kb on - strand, within EX31_RS07875 at 56.737 kb on + strand, within EX31_RS07875 at 56.738 kb on - strand, within EX31_RS07875 at 56.738 kb on - strand, within EX31_RS07875 at 56.740 kb on + strand, within EX31_RS07875 at 56.741 kb on - strand, within EX31_RS07875 at 56.741 kb on - strand, within EX31_RS07875 at 56.741 kb on - strand, within EX31_RS07875 at 56.784 kb on + strand, within EX31_RS07875 at 56.784 kb on + strand, within EX31_RS07875 at 56.784 kb on + strand, within EX31_RS07875 at 56.784 kb on + strand, within EX31_RS07875 at 56.784 kb on + strand, within EX31_RS07875 at 56.785 kb on - strand, within EX31_RS07875 at 56.785 kb on - strand, within EX31_RS07875 at 56.785 kb on - strand, within EX31_RS07875 at 56.785 kb on - strand, within EX31_RS07875 at 56.785 kb on - strand, within EX31_RS07875 at 56.785 kb on - strand, within EX31_RS07875 at 56.785 kb on - strand, within EX31_RS07875 at 56.892 kb on + strand, within EX31_RS07875 at 56.893 kb on - strand, within EX31_RS07875 at 56.893 kb on - strand, within EX31_RS07875 at 56.893 kb on - strand, within EX31_RS07875 at 56.893 kb on - strand, within EX31_RS07875 at 56.893 kb on - strand, within EX31_RS07875 at 56.893 kb on - strand, within EX31_RS07875 at 56.903 kb on - strand, within EX31_RS07875 at 56.929 kb on - strand, within EX31_RS07875 at 56.992 kb on + strand, within EX31_RS07875
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 54,781 - +2.7 54,781 - +0.2 54,859 - +0.6 54,880 + -0.4 54,880 + -0.2 54,880 + -2.9 54,881 - +0.2 54,881 - +1.2 54,881 - +0.6 54,892 + -1.2 54,892 + +0.2 54,893 - +0.1 54,917 + -0.0 54,918 - +0.2 55,034 - EX31_RS07865 0.20 -0.8 55,073 + EX31_RS07865 0.30 -0.3 55,073 + EX31_RS07865 0.30 +0.2 55,074 - EX31_RS07865 0.30 -0.1 55,074 - EX31_RS07865 0.30 -0.9 55,074 - EX31_RS07865 0.30 -1.6 55,119 + EX31_RS07865 0.42 -0.7 55,119 + EX31_RS07865 0.42 -0.9 55,119 + EX31_RS07865 0.42 -0.7 55,120 - EX31_RS07865 0.42 -0.1 55,120 - EX31_RS07865 0.42 -0.3 55,150 - EX31_RS07865 0.50 -0.5 55,182 + EX31_RS07865 0.59 +0.3 55,182 + EX31_RS07865 0.59 +0.6 55,183 - EX31_RS07865 0.59 +1.5 55,218 + EX31_RS07865 0.68 +1.3 55,218 + EX31_RS07865 0.68 -0.1 55,218 + EX31_RS07865 0.68 +0.2 55,218 + EX31_RS07865 0.68 +0.4 55,218 + EX31_RS07865 0.68 -0.4 55,218 + EX31_RS07865 0.68 +0.4 55,328 + -1.2 55,329 - +0.6 55,339 + +0.8 55,339 + -0.1 55,339 + -0.7 55,339 + -0.4 55,339 + +0.2 55,339 + +0.9 55,339 + -0.6 55,339 + +1.0 55,340 - -0.3 55,340 - +1.0 55,340 - -0.2 55,340 - +0.4 55,340 - -1.0 55,340 - -1.1 55,340 - -0.2 55,340 - -0.9 55,340 - +0.2 55,340 - +0.6 55,340 - -0.1 55,340 - +0.2 55,352 + +0.6 55,353 - -0.5 55,410 + -3.5 55,410 + -2.1 55,410 + +0.2 55,411 - -2.7 55,411 - -0.5 55,411 - +0.3 55,411 - -2.1 55,436 - -1.3 55,439 - -1.8 55,623 + EX31_RS07870 0.36 -0.8 55,623 + EX31_RS07870 0.36 -2.7 55,623 + EX31_RS07870 0.36 -2.3 55,623 + EX31_RS07870 0.36 -2.5 55,624 - EX31_RS07870 0.36 -2.7 55,624 - EX31_RS07870 0.36 -3.2 55,624 - EX31_RS07870 0.36 -1.5 55,624 - EX31_RS07870 0.36 -1.9 55,738 + EX31_RS07870 0.54 -0.5 55,739 - EX31_RS07870 0.54 -1.7 55,739 - EX31_RS07870 0.54 -2.8 55,810 - EX31_RS07870 0.66 -2.3 55,826 + EX31_RS07870 0.68 -1.7 55,826 + EX31_RS07870 0.68 -2.0 55,826 + EX31_RS07870 0.68 -1.3 55,826 + EX31_RS07870 0.68 -3.4 55,826 + EX31_RS07870 0.68 -2.5 55,826 + EX31_RS07870 0.68 -2.5 55,826 + EX31_RS07870 0.68 -2.5 55,826 + EX31_RS07870 0.68 -3.4 55,826 + EX31_RS07870 0.68 +0.0 55,827 - EX31_RS07870 0.69 -4.1 55,827 - EX31_RS07870 0.69 -1.7 55,827 - EX31_RS07870 0.69 -1.1 55,847 + EX31_RS07870 0.72 -2.7 55,847 + EX31_RS07870 0.72 -1.6 55,847 + EX31_RS07870 0.72 -2.5 55,996 + -2.4 56,025 + -2.3 56,025 + -2.3 56,025 + -2.0 56,025 + -0.7 56,025 + -2.1 56,026 - -2.9 56,109 + -2.0 56,109 + -2.5 56,109 + -2.3 56,109 + -0.5 56,109 + -2.5 56,109 + -1.7 56,110 - -2.0 56,110 - -1.2 56,110 - -2.6 56,110 - -3.2 56,110 - -0.8 56,114 - -0.3 56,128 - -1.6 56,129 + +2.5 56,129 + -0.9 56,129 + -2.6 56,129 + -2.3 56,129 + -2.0 56,129 + -1.7 56,129 + -3.2 56,129 + -1.3 56,129 + -2.1 56,129 + -3.0 56,129 + -5.1 56,129 + -0.3 56,129 + -0.7 56,129 + -1.2 56,129 + -2.6 56,129 + -2.9 56,129 + -2.8 56,130 - -2.6 56,130 - +0.9 56,130 - -2.1 56,130 - -2.5 56,130 - -1.6 56,130 - -2.4 56,130 - -2.7 56,130 - -1.2 56,130 - -1.9 56,130 - -1.8 56,130 - -0.8 56,130 - -0.9 56,130 - -0.9 56,130 - -0.2 56,130 - -3.1 56,130 - -1.2 56,171 - EX31_RS07875 0.10 -1.4 56,171 - EX31_RS07875 0.10 -3.5 56,226 + EX31_RS07875 0.14 -3.0 56,226 + EX31_RS07875 0.14 -2.8 56,226 + EX31_RS07875 0.14 -1.4 56,226 + EX31_RS07875 0.14 -0.7 56,227 - EX31_RS07875 0.14 -2.9 56,227 - EX31_RS07875 0.14 -3.7 56,227 - EX31_RS07875 0.14 -2.6 56,230 + EX31_RS07875 0.14 -2.9 56,230 + EX31_RS07875 0.14 -3.6 56,230 + EX31_RS07875 0.14 -1.6 56,321 - EX31_RS07875 0.21 -3.1 56,321 - EX31_RS07875 0.21 -2.6 56,334 + EX31_RS07875 0.22 -1.4 56,335 - EX31_RS07875 0.22 -1.9 56,335 - EX31_RS07875 0.22 -4.1 56,342 - EX31_RS07875 0.22 -0.7 56,388 + EX31_RS07875 0.25 -2.7 56,389 - EX31_RS07875 0.26 -1.7 56,389 - EX31_RS07875 0.26 -3.3 56,501 - EX31_RS07875 0.33 -2.7 56,590 + EX31_RS07875 0.40 -1.5 56,590 + EX31_RS07875 0.40 -2.1 56,591 - EX31_RS07875 0.40 -0.7 56,591 - EX31_RS07875 0.40 -1.5 56,591 - EX31_RS07875 0.40 -1.3 56,591 - EX31_RS07875 0.40 -2.0 56,591 - EX31_RS07875 0.40 +0.2 56,612 + EX31_RS07875 0.41 -2.1 56,612 + EX31_RS07875 0.41 -0.3 56,613 - EX31_RS07875 0.41 -2.4 56,613 - EX31_RS07875 0.41 -2.0 56,613 - EX31_RS07875 0.41 -1.8 56,613 - EX31_RS07875 0.41 -2.4 56,664 + EX31_RS07875 0.45 -1.5 56,665 - EX31_RS07875 0.45 -2.4 56,697 + EX31_RS07875 0.47 -2.4 56,697 + EX31_RS07875 0.47 -2.9 56,698 - EX31_RS07875 0.47 -1.1 56,698 - EX31_RS07875 0.47 -2.9 56,737 + EX31_RS07875 0.50 -1.9 56,738 - EX31_RS07875 0.50 -2.6 56,738 - EX31_RS07875 0.50 -1.3 56,740 + EX31_RS07875 0.50 -1.9 56,741 - EX31_RS07875 0.50 -0.3 56,741 - EX31_RS07875 0.50 -3.3 56,741 - EX31_RS07875 0.50 -1.4 56,784 + EX31_RS07875 0.53 -2.0 56,784 + EX31_RS07875 0.53 -1.8 56,784 + EX31_RS07875 0.53 -0.1 56,784 + EX31_RS07875 0.53 -1.8 56,784 + EX31_RS07875 0.53 -3.5 56,785 - EX31_RS07875 0.53 -1.2 56,785 - EX31_RS07875 0.53 -2.6 56,785 - EX31_RS07875 0.53 -2.8 56,785 - EX31_RS07875 0.53 -2.8 56,785 - EX31_RS07875 0.53 -3.1 56,785 - EX31_RS07875 0.53 -1.3 56,785 - EX31_RS07875 0.53 -2.5 56,892 + EX31_RS07875 0.61 -2.3 56,893 - EX31_RS07875 0.61 -2.1 56,893 - EX31_RS07875 0.61 -2.3 56,893 - EX31_RS07875 0.61 -0.5 56,893 - EX31_RS07875 0.61 -2.5 56,893 - EX31_RS07875 0.61 -2.7 56,893 - EX31_RS07875 0.61 -0.5 56,903 - EX31_RS07875 0.62 -1.0 56,929 - EX31_RS07875 0.63 -3.4 56,992 + EX31_RS07875 0.68 -3.5
Or see this region's nucleotide sequence