Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06820 and EX31_RS06825 are separated by 13 nucleotides EX31_RS06825 and EX31_RS06830 are separated by 38 nucleotides EX31_RS06830 and EX31_RS06835 are separated by 21 nucleotides EX31_RS06835 and EX31_RS06840 overlap by 8 nucleotides
EX31_RS06820: EX31_RS06820 - amino acid ABC transporter permease, at 152,062 to 152,934
_RS06820
EX31_RS06825: EX31_RS06825 - amino acid ABC transporter ATP-binding protein, at 152,948 to 153,712
_RS06825
EX31_RS06830: EX31_RS06830 - ABC transporter substrate-binding protein, at 153,751 to 154,572
_RS06830
EX31_RS06835: EX31_RS06835 - polysaccharide deacetylase, at 154,594 to 155,448
_RS06835
EX31_RS06840: EX31_RS06840 - polysaccharide deacetylase, at 155,441 to 156,343
_RS06840
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 152.776 kb on + strand, within EX31_RS06820 at 152.776 kb on + strand, within EX31_RS06820 at 152.777 kb on - strand, within EX31_RS06820 at 152.794 kb on + strand, within EX31_RS06820 at 152.794 kb on + strand, within EX31_RS06820 at 152.795 kb on - strand, within EX31_RS06820 at 152.896 kb on + strand at 152.896 kb on + strand at 152.959 kb on + strand at 152.980 kb on + strand at 152.984 kb on + strand at 152.984 kb on + strand at 152.985 kb on - strand at 152.985 kb on - strand at 153.231 kb on + strand, within EX31_RS06825 at 153.231 kb on + strand, within EX31_RS06825 at 153.232 kb on - strand, within EX31_RS06825 at 153.370 kb on + strand, within EX31_RS06825 at 153.573 kb on + strand, within EX31_RS06825 at 153.574 kb on - strand, within EX31_RS06825 at 153.630 kb on + strand, within EX31_RS06825 at 153.631 kb on - strand, within EX31_RS06825 at 153.652 kb on + strand at 153.849 kb on + strand, within EX31_RS06830 at 154.091 kb on - strand, within EX31_RS06830 at 154.093 kb on + strand, within EX31_RS06830 at 154.093 kb on + strand, within EX31_RS06830 at 154.093 kb on + strand, within EX31_RS06830 at 154.114 kb on + strand, within EX31_RS06830 at 154.115 kb on - strand, within EX31_RS06830 at 154.115 kb on - strand, within EX31_RS06830 at 154.115 kb on - strand, within EX31_RS06830 at 154.117 kb on - strand, within EX31_RS06830 at 154.227 kb on + strand, within EX31_RS06830 at 154.227 kb on + strand, within EX31_RS06830 at 154.228 kb on - strand, within EX31_RS06830 at 154.351 kb on + strand, within EX31_RS06830 at 154.378 kb on + strand, within EX31_RS06830 at 154.379 kb on - strand, within EX31_RS06830 at 154.622 kb on + strand at 154.622 kb on + strand at 154.723 kb on + strand, within EX31_RS06835 at 154.886 kb on - strand, within EX31_RS06835 at 155.066 kb on - strand, within EX31_RS06835 at 155.066 kb on - strand, within EX31_RS06835 at 155.076 kb on + strand, within EX31_RS06835 at 155.077 kb on - strand, within EX31_RS06835 at 155.077 kb on - strand, within EX31_RS06835 at 155.098 kb on + strand, within EX31_RS06835 at 155.175 kb on + strand, within EX31_RS06835 at 155.176 kb on - strand, within EX31_RS06835 at 155.182 kb on + strand, within EX31_RS06835 at 155.183 kb on - strand, within EX31_RS06835 at 155.183 kb on - strand, within EX31_RS06835 at 155.214 kb on - strand, within EX31_RS06835 at 155.230 kb on + strand, within EX31_RS06835 at 155.340 kb on + strand, within EX31_RS06835 at 155.341 kb on - strand, within EX31_RS06835 at 155.345 kb on + strand, within EX31_RS06835 at 155.346 kb on - strand, within EX31_RS06835 at 155.346 kb on - strand, within EX31_RS06835 at 155.488 kb on + strand at 155.489 kb on - strand at 155.489 kb on - strand at 155.506 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 152,776 + EX31_RS06820 0.82 +1.0 152,776 + EX31_RS06820 0.82 +0.9 152,777 - EX31_RS06820 0.82 -0.5 152,794 + EX31_RS06820 0.84 -2.1 152,794 + EX31_RS06820 0.84 +0.5 152,795 - EX31_RS06820 0.84 +1.0 152,896 + -0.8 152,896 + +0.7 152,959 + +1.6 152,980 + -1.0 152,984 + +0.8 152,984 + -0.2 152,985 - -0.4 152,985 - -0.7 153,231 + EX31_RS06825 0.37 +0.4 153,231 + EX31_RS06825 0.37 -0.3 153,232 - EX31_RS06825 0.37 +0.9 153,370 + EX31_RS06825 0.55 -1.3 153,573 + EX31_RS06825 0.82 +0.6 153,574 - EX31_RS06825 0.82 -0.3 153,630 + EX31_RS06825 0.89 -0.3 153,631 - EX31_RS06825 0.89 +2.0 153,652 + +1.3 153,849 + EX31_RS06830 0.12 -0.0 154,091 - EX31_RS06830 0.41 -0.1 154,093 + EX31_RS06830 0.42 -1.1 154,093 + EX31_RS06830 0.42 -1.9 154,093 + EX31_RS06830 0.42 -1.7 154,114 + EX31_RS06830 0.44 -0.7 154,115 - EX31_RS06830 0.44 +1.4 154,115 - EX31_RS06830 0.44 +1.1 154,115 - EX31_RS06830 0.44 -1.4 154,117 - EX31_RS06830 0.45 +0.4 154,227 + EX31_RS06830 0.58 +0.4 154,227 + EX31_RS06830 0.58 -0.3 154,228 - EX31_RS06830 0.58 -2.2 154,351 + EX31_RS06830 0.73 +0.2 154,378 + EX31_RS06830 0.76 -1.8 154,379 - EX31_RS06830 0.76 -1.3 154,622 + +0.6 154,622 + -0.6 154,723 + EX31_RS06835 0.15 -1.3 154,886 - EX31_RS06835 0.34 -0.6 155,066 - EX31_RS06835 0.55 +2.0 155,066 - EX31_RS06835 0.55 +0.9 155,076 + EX31_RS06835 0.56 -2.8 155,077 - EX31_RS06835 0.56 +0.8 155,077 - EX31_RS06835 0.56 -2.0 155,098 + EX31_RS06835 0.59 -1.2 155,175 + EX31_RS06835 0.68 +1.8 155,176 - EX31_RS06835 0.68 +0.7 155,182 + EX31_RS06835 0.69 +0.8 155,183 - EX31_RS06835 0.69 -0.1 155,183 - EX31_RS06835 0.69 -1.3 155,214 - EX31_RS06835 0.73 +2.0 155,230 + EX31_RS06835 0.74 -1.0 155,340 + EX31_RS06835 0.87 -1.2 155,341 - EX31_RS06835 0.87 +0.6 155,345 + EX31_RS06835 0.88 +0.8 155,346 - EX31_RS06835 0.88 +0.2 155,346 - EX31_RS06835 0.88 +1.7 155,488 + +0.5 155,489 - -0.2 155,489 - +0.2 155,506 + +1.1
Or see this region's nucleotide sequence