Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06345 and EX31_RS06350 are separated by 145 nucleotides EX31_RS06350 and EX31_RS06355 are separated by 64 nucleotides
EX31_RS06345: EX31_RS06345 - L-Ala-D/L-Glu epimerase, at 31,667 to 32,644
_RS06345
EX31_RS06350: EX31_RS06350 - peptide ABC transporter substrate-binding protein, at 32,790 to 34,424
_RS06350
EX31_RS06355: EX31_RS06355 - MFS transporter, at 34,489 to 35,691
_RS06355
Position (kb) 32
33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 31.817 kb on + strand, within EX31_RS06345 at 31.872 kb on + strand, within EX31_RS06345 at 31.872 kb on + strand, within EX31_RS06345 at 31.872 kb on + strand, within EX31_RS06345 at 31.872 kb on + strand, within EX31_RS06345 at 31.873 kb on - strand, within EX31_RS06345 at 31.873 kb on - strand, within EX31_RS06345 at 31.873 kb on - strand, within EX31_RS06345 at 31.873 kb on - strand, within EX31_RS06345 at 31.908 kb on + strand, within EX31_RS06345 at 32.040 kb on + strand, within EX31_RS06345 at 32.040 kb on + strand, within EX31_RS06345 at 32.040 kb on + strand, within EX31_RS06345 at 32.041 kb on - strand, within EX31_RS06345 at 32.041 kb on - strand, within EX31_RS06345 at 32.041 kb on - strand, within EX31_RS06345 at 32.041 kb on - strand, within EX31_RS06345 at 32.404 kb on + strand, within EX31_RS06345 at 32.405 kb on - strand, within EX31_RS06345 at 32.545 kb on + strand, within EX31_RS06345 at 32.552 kb on - strand at 32.739 kb on - strand at 32.763 kb on + strand at 32.763 kb on + strand at 32.818 kb on + strand at 32.930 kb on + strand at 33.086 kb on - strand, within EX31_RS06350 at 33.100 kb on + strand, within EX31_RS06350 at 33.157 kb on - strand, within EX31_RS06350 at 33.158 kb on + strand, within EX31_RS06350 at 33.158 kb on + strand, within EX31_RS06350 at 33.158 kb on + strand, within EX31_RS06350 at 33.158 kb on + strand, within EX31_RS06350 at 33.159 kb on - strand, within EX31_RS06350 at 33.270 kb on - strand, within EX31_RS06350 at 33.444 kb on + strand, within EX31_RS06350 at 33.501 kb on + strand, within EX31_RS06350 at 33.627 kb on + strand, within EX31_RS06350 at 33.663 kb on + strand, within EX31_RS06350 at 33.687 kb on + strand, within EX31_RS06350 at 33.687 kb on + strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.688 kb on - strand, within EX31_RS06350 at 33.690 kb on + strand, within EX31_RS06350 at 33.693 kb on + strand, within EX31_RS06350 at 33.693 kb on + strand, within EX31_RS06350 at 33.693 kb on + strand, within EX31_RS06350 at 33.758 kb on + strand, within EX31_RS06350 at 33.758 kb on + strand, within EX31_RS06350 at 33.822 kb on - strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.963 kb on + strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.964 kb on - strand, within EX31_RS06350 at 33.965 kb on + strand, within EX31_RS06350 at 33.966 kb on - strand, within EX31_RS06350 at 34.068 kb on + strand, within EX31_RS06350 at 34.128 kb on + strand, within EX31_RS06350 at 34.129 kb on - strand, within EX31_RS06350 at 34.211 kb on + strand, within EX31_RS06350 at 34.267 kb on - strand at 34.286 kb on + strand at 34.287 kb on - strand at 34.318 kb on - strand at 34.324 kb on - strand at 34.363 kb on - strand at 34.393 kb on - strand at 34.407 kb on + strand at 34.407 kb on + strand at 34.407 kb on + strand at 34.407 kb on + strand at 34.426 kb on - strand at 34.478 kb on + strand at 34.513 kb on + strand at 34.679 kb on + strand, within EX31_RS06355 at 34.680 kb on - strand, within EX31_RS06355 at 34.716 kb on + strand, within EX31_RS06355 at 34.717 kb on - strand, within EX31_RS06355 at 34.717 kb on - strand, within EX31_RS06355 at 34.720 kb on + strand, within EX31_RS06355 at 34.720 kb on + strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.721 kb on - strand, within EX31_RS06355 at 34.888 kb on + strand, within EX31_RS06355 at 34.962 kb on + strand, within EX31_RS06355 at 34.963 kb on - strand, within EX31_RS06355 at 35.040 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.171 kb on + strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.172 kb on - strand, within EX31_RS06355 at 35.192 kb on + strand, within EX31_RS06355 at 35.192 kb on + strand, within EX31_RS06355 at 35.193 kb on - strand, within EX31_RS06355
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 31,817 + EX31_RS06345 0.15 +0.1 31,872 + EX31_RS06345 0.21 -0.0 31,872 + EX31_RS06345 0.21 +0.2 31,872 + EX31_RS06345 0.21 +1.0 31,872 + EX31_RS06345 0.21 -0.0 31,873 - EX31_RS06345 0.21 +0.3 31,873 - EX31_RS06345 0.21 -0.2 31,873 - EX31_RS06345 0.21 +1.0 31,873 - EX31_RS06345 0.21 -0.6 31,908 + EX31_RS06345 0.25 +0.5 32,040 + EX31_RS06345 0.38 -0.6 32,040 + EX31_RS06345 0.38 +1.6 32,040 + EX31_RS06345 0.38 +1.6 32,041 - EX31_RS06345 0.38 -1.5 32,041 - EX31_RS06345 0.38 +3.2 32,041 - EX31_RS06345 0.38 +1.4 32,041 - EX31_RS06345 0.38 +0.5 32,404 + EX31_RS06345 0.75 +2.0 32,405 - EX31_RS06345 0.75 -0.9 32,545 + EX31_RS06345 0.90 +2.4 32,552 - +0.8 32,739 - +0.4 32,763 + -0.3 32,763 + -0.2 32,818 + -0.4 32,930 + +2.0 33,086 - EX31_RS06350 0.18 -0.1 33,100 + EX31_RS06350 0.19 -0.8 33,157 - EX31_RS06350 0.22 -2.8 33,158 + EX31_RS06350 0.23 -0.5 33,158 + EX31_RS06350 0.23 +1.0 33,158 + EX31_RS06350 0.23 -0.3 33,158 + EX31_RS06350 0.23 -0.2 33,159 - EX31_RS06350 0.23 +0.6 33,270 - EX31_RS06350 0.29 -0.2 33,444 + EX31_RS06350 0.40 -0.3 33,501 + EX31_RS06350 0.43 +2.4 33,627 + EX31_RS06350 0.51 +0.0 33,663 + EX31_RS06350 0.53 +0.7 33,687 + EX31_RS06350 0.55 +1.6 33,687 + EX31_RS06350 0.55 +0.9 33,688 - EX31_RS06350 0.55 +0.1 33,688 - EX31_RS06350 0.55 -1.2 33,688 - EX31_RS06350 0.55 +2.0 33,688 - EX31_RS06350 0.55 -0.1 33,688 - EX31_RS06350 0.55 -0.0 33,690 + EX31_RS06350 0.55 -0.0 33,693 + EX31_RS06350 0.55 -0.0 33,693 + EX31_RS06350 0.55 +0.4 33,693 + EX31_RS06350 0.55 +0.4 33,758 + EX31_RS06350 0.59 +0.6 33,758 + EX31_RS06350 0.59 +1.0 33,822 - EX31_RS06350 0.63 -0.6 33,963 + EX31_RS06350 0.72 -1.0 33,963 + EX31_RS06350 0.72 +0.3 33,963 + EX31_RS06350 0.72 +0.2 33,963 + EX31_RS06350 0.72 -1.2 33,964 - EX31_RS06350 0.72 -1.5 33,964 - EX31_RS06350 0.72 +0.3 33,964 - EX31_RS06350 0.72 +2.0 33,964 - EX31_RS06350 0.72 +1.2 33,965 + EX31_RS06350 0.72 +0.4 33,966 - EX31_RS06350 0.72 -1.0 34,068 + EX31_RS06350 0.78 +0.6 34,128 + EX31_RS06350 0.82 -0.2 34,129 - EX31_RS06350 0.82 +0.6 34,211 + EX31_RS06350 0.87 +0.7 34,267 - -0.3 34,286 + +0.2 34,287 - -1.0 34,318 - -0.3 34,324 - +1.0 34,363 - +0.2 34,393 - +1.3 34,407 + +1.3 34,407 + -0.0 34,407 + +0.1 34,407 + +0.4 34,426 - -0.8 34,478 + -1.0 34,513 + +2.0 34,679 + EX31_RS06355 0.16 +1.4 34,680 - EX31_RS06355 0.16 +1.4 34,716 + EX31_RS06355 0.19 +1.4 34,717 - EX31_RS06355 0.19 +1.0 34,717 - EX31_RS06355 0.19 -0.2 34,720 + EX31_RS06355 0.19 +1.0 34,720 + EX31_RS06355 0.19 -1.7 34,721 - EX31_RS06355 0.19 +0.9 34,721 - EX31_RS06355 0.19 +1.6 34,721 - EX31_RS06355 0.19 -1.8 34,721 - EX31_RS06355 0.19 +0.4 34,888 + EX31_RS06355 0.33 -0.3 34,962 + EX31_RS06355 0.39 -0.4 34,963 - EX31_RS06355 0.39 +1.4 35,040 + EX31_RS06355 0.46 +0.2 35,171 + EX31_RS06355 0.57 +1.0 35,171 + EX31_RS06355 0.57 -1.2 35,171 + EX31_RS06355 0.57 +0.1 35,171 + EX31_RS06355 0.57 -0.5 35,172 - EX31_RS06355 0.57 -0.5 35,172 - EX31_RS06355 0.57 -0.5 35,172 - EX31_RS06355 0.57 +0.6 35,172 - EX31_RS06355 0.57 +0.4 35,172 - EX31_RS06355 0.57 +0.5 35,192 + EX31_RS06355 0.58 +1.1 35,192 + EX31_RS06355 0.58 -0.6 35,193 - EX31_RS06355 0.59 -0.3
Or see this region's nucleotide sequence