Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06065 and EX31_RS06070 are separated by 94 nucleotides EX31_RS06070 and EX31_RS06075 are separated by 216 nucleotides
EX31_RS06065: EX31_RS06065 - phosphate ABC transporter permease PstC, at 373,160 to 374,116
_RS06065
EX31_RS06070: EX31_RS06070 - phosphate ABC transporter substrate-binding protein PstS, at 374,211 to 375,251
_RS06070
EX31_RS06075: EX31_RS06075 - 23S rRNA pseudouridine(2604) synthase RluF, at 375,468 to 376,340
_RS06075
Position (kb) 374
375
376 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 373.220 kb on - strand at 373.220 kb on - strand at 373.220 kb on - strand at 373.268 kb on - strand, within EX31_RS06065 at 373.268 kb on - strand, within EX31_RS06065 at 373.268 kb on - strand, within EX31_RS06065 at 373.268 kb on - strand, within EX31_RS06065 at 373.268 kb on - strand, within EX31_RS06065 at 373.268 kb on - strand, within EX31_RS06065 at 373.268 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.316 kb on - strand, within EX31_RS06065 at 373.319 kb on - strand, within EX31_RS06065 at 373.319 kb on - strand, within EX31_RS06065 at 373.319 kb on - strand, within EX31_RS06065 at 373.319 kb on - strand, within EX31_RS06065 at 373.319 kb on - strand, within EX31_RS06065 at 373.360 kb on - strand, within EX31_RS06065 at 373.360 kb on - strand, within EX31_RS06065 at 373.360 kb on - strand, within EX31_RS06065 at 373.376 kb on - strand, within EX31_RS06065 at 373.419 kb on - strand, within EX31_RS06065 at 373.419 kb on - strand, within EX31_RS06065 at 373.487 kb on - strand, within EX31_RS06065 at 373.487 kb on - strand, within EX31_RS06065 at 373.487 kb on - strand, within EX31_RS06065 at 373.563 kb on - strand, within EX31_RS06065 at 373.563 kb on - strand, within EX31_RS06065 at 373.563 kb on - strand, within EX31_RS06065 at 373.563 kb on - strand, within EX31_RS06065 at 373.563 kb on - strand, within EX31_RS06065 at 373.567 kb on - strand, within EX31_RS06065 at 373.580 kb on - strand, within EX31_RS06065 at 373.610 kb on - strand, within EX31_RS06065 at 373.668 kb on - strand, within EX31_RS06065 at 373.693 kb on - strand, within EX31_RS06065 at 373.745 kb on - strand, within EX31_RS06065 at 373.745 kb on - strand, within EX31_RS06065 at 373.868 kb on - strand, within EX31_RS06065 at 374.021 kb on - strand at 374.021 kb on - strand at 374.021 kb on - strand at 374.021 kb on - strand at 374.021 kb on - strand at 374.021 kb on - strand at 374.021 kb on - strand at 374.021 kb on - strand at 374.025 kb on - strand at 374.025 kb on - strand at 374.025 kb on - strand at 374.107 kb on - strand at 374.130 kb on - strand at 374.130 kb on - strand at 374.141 kb on - strand at 374.141 kb on - strand at 374.141 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.184 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.189 kb on - strand at 374.213 kb on - strand at 374.213 kb on - strand at 374.213 kb on - strand at 374.216 kb on - strand at 374.216 kb on - strand at 374.216 kb on - strand at 374.275 kb on - strand at 374.275 kb on - strand at 374.275 kb on - strand at 374.336 kb on - strand, within EX31_RS06070 at 374.336 kb on - strand, within EX31_RS06070 at 374.548 kb on - strand, within EX31_RS06070 at 374.561 kb on - strand, within EX31_RS06070 at 374.561 kb on - strand, within EX31_RS06070 at 374.561 kb on - strand, within EX31_RS06070 at 374.585 kb on - strand, within EX31_RS06070 at 374.591 kb on - strand, within EX31_RS06070 at 374.591 kb on - strand, within EX31_RS06070 at 374.591 kb on - strand, within EX31_RS06070 at 374.591 kb on - strand, within EX31_RS06070 at 374.591 kb on - strand, within EX31_RS06070 at 374.591 kb on - strand, within EX31_RS06070 at 374.591 kb on - strand, within EX31_RS06070 at 374.596 kb on - strand, within EX31_RS06070 at 374.596 kb on - strand, within EX31_RS06070 at 374.596 kb on - strand, within EX31_RS06070 at 374.596 kb on - strand, within EX31_RS06070 at 374.596 kb on - strand, within EX31_RS06070 at 374.596 kb on - strand, within EX31_RS06070 at 374.600 kb on - strand, within EX31_RS06070 at 374.679 kb on - strand, within EX31_RS06070 at 374.679 kb on - strand, within EX31_RS06070 at 374.685 kb on - strand, within EX31_RS06070 at 374.685 kb on - strand, within EX31_RS06070 at 374.685 kb on - strand, within EX31_RS06070 at 374.735 kb on - strand, within EX31_RS06070 at 374.757 kb on - strand, within EX31_RS06070 at 374.867 kb on - strand, within EX31_RS06070 at 374.867 kb on - strand, within EX31_RS06070 at 374.867 kb on - strand, within EX31_RS06070 at 374.867 kb on - strand, within EX31_RS06070 at 374.867 kb on - strand, within EX31_RS06070 at 374.871 kb on - strand, within EX31_RS06070 at 374.871 kb on - strand, within EX31_RS06070 at 374.871 kb on - strand, within EX31_RS06070 at 374.871 kb on - strand, within EX31_RS06070 at 374.871 kb on - strand, within EX31_RS06070 at 374.958 kb on - strand, within EX31_RS06070 at 375.079 kb on + strand, within EX31_RS06070 at 375.080 kb on - strand, within EX31_RS06070 at 375.126 kb on - strand, within EX31_RS06070 at 375.126 kb on - strand, within EX31_RS06070 at 375.126 kb on - strand, within EX31_RS06070 at 375.130 kb on + strand, within EX31_RS06070 at 375.131 kb on - strand, within EX31_RS06070 at 375.131 kb on - strand, within EX31_RS06070 at 375.217 kb on - strand at 375.217 kb on - strand at 375.217 kb on - strand at 375.237 kb on - strand at 375.237 kb on - strand at 375.297 kb on - strand at 375.300 kb on + strand at 375.300 kb on + strand at 375.300 kb on + strand at 375.300 kb on + strand at 375.301 kb on - strand at 375.301 kb on - strand at 375.301 kb on - strand at 375.301 kb on - strand at 375.325 kb on + strand at 375.326 kb on - strand at 375.326 kb on - strand at 375.326 kb on - strand at 375.326 kb on - strand at 375.335 kb on + strand at 375.336 kb on - strand at 375.336 kb on - strand at 375.344 kb on + strand at 375.351 kb on + strand at 375.351 kb on + strand at 375.352 kb on - strand at 375.352 kb on - strand at 375.352 kb on - strand at 375.356 kb on + strand at 375.356 kb on + strand at 375.356 kb on + strand at 375.357 kb on - strand at 375.358 kb on + strand at 375.358 kb on + strand at 375.358 kb on + strand at 375.358 kb on + strand at 375.359 kb on - strand at 375.359 kb on - strand at 375.359 kb on - strand at 375.417 kb on + strand at 375.417 kb on + strand at 375.435 kb on + strand at 375.436 kb on - strand at 375.436 kb on - strand at 375.438 kb on + strand at 375.438 kb on + strand at 375.439 kb on - strand at 375.439 kb on - strand at 375.439 kb on - strand at 375.439 kb on - strand at 375.470 kb on - strand at 375.470 kb on - strand at 375.470 kb on - strand at 375.642 kb on - strand, within EX31_RS06075 at 375.642 kb on - strand, within EX31_RS06075 at 375.693 kb on + strand, within EX31_RS06075 at 375.694 kb on - strand, within EX31_RS06075 at 375.717 kb on - strand, within EX31_RS06075 at 375.717 kb on - strand, within EX31_RS06075 at 375.738 kb on - strand, within EX31_RS06075 at 375.738 kb on - strand, within EX31_RS06075 at 375.738 kb on - strand, within EX31_RS06075 at 375.761 kb on + strand, within EX31_RS06075 at 375.761 kb on + strand, within EX31_RS06075 at 375.761 kb on + strand, within EX31_RS06075 at 375.762 kb on - strand, within EX31_RS06075 at 375.785 kb on + strand, within EX31_RS06075 at 375.786 kb on - strand, within EX31_RS06075 at 375.804 kb on - strand, within EX31_RS06075 at 375.894 kb on - strand, within EX31_RS06075 at 375.894 kb on - strand, within EX31_RS06075 at 375.929 kb on + strand, within EX31_RS06075 at 375.929 kb on + strand, within EX31_RS06075 at 375.929 kb on + strand, within EX31_RS06075 at 375.929 kb on + strand, within EX31_RS06075 at 375.929 kb on + strand, within EX31_RS06075 at 375.930 kb on - strand, within EX31_RS06075 at 375.930 kb on - strand, within EX31_RS06075 at 375.931 kb on + strand, within EX31_RS06075 at 375.931 kb on + strand, within EX31_RS06075 at 375.931 kb on + strand, within EX31_RS06075 at 375.932 kb on - strand, within EX31_RS06075 at 375.932 kb on - strand, within EX31_RS06075 at 375.932 kb on - strand, within EX31_RS06075 at 375.932 kb on - strand, within EX31_RS06075 at 375.932 kb on - strand, within EX31_RS06075 at 376.114 kb on - strand, within EX31_RS06075 at 376.133 kb on + strand, within EX31_RS06075 at 376.133 kb on + strand, within EX31_RS06075 at 376.134 kb on - strand, within EX31_RS06075 at 376.134 kb on - strand, within EX31_RS06075 at 376.134 kb on - strand, within EX31_RS06075 at 376.134 kb on - strand, within EX31_RS06075 at 376.167 kb on - strand, within EX31_RS06075 at 376.239 kb on - strand, within EX31_RS06075
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 373,220 - -0.5 373,220 - -0.4 373,220 - -0.9 373,268 - EX31_RS06065 0.11 -1.0 373,268 - EX31_RS06065 0.11 -1.8 373,268 - EX31_RS06065 0.11 -0.4 373,268 - EX31_RS06065 0.11 -1.1 373,268 - EX31_RS06065 0.11 -0.8 373,268 - EX31_RS06065 0.11 -2.3 373,268 - EX31_RS06065 0.11 -2.5 373,316 - EX31_RS06065 0.16 -1.5 373,316 - EX31_RS06065 0.16 -2.3 373,316 - EX31_RS06065 0.16 +0.1 373,316 - EX31_RS06065 0.16 -0.1 373,316 - EX31_RS06065 0.16 -3.1 373,316 - EX31_RS06065 0.16 -2.0 373,316 - EX31_RS06065 0.16 +0.6 373,316 - EX31_RS06065 0.16 -1.3 373,316 - EX31_RS06065 0.16 -2.7 373,316 - EX31_RS06065 0.16 -1.3 373,319 - EX31_RS06065 0.17 -2.1 373,319 - EX31_RS06065 0.17 -0.9 373,319 - EX31_RS06065 0.17 -0.6 373,319 - EX31_RS06065 0.17 -3.3 373,319 - EX31_RS06065 0.17 -1.8 373,360 - EX31_RS06065 0.21 -2.6 373,360 - EX31_RS06065 0.21 -0.6 373,360 - EX31_RS06065 0.21 -2.7 373,376 - EX31_RS06065 0.23 -2.7 373,419 - EX31_RS06065 0.27 -0.8 373,419 - EX31_RS06065 0.27 -1.6 373,487 - EX31_RS06065 0.34 -2.6 373,487 - EX31_RS06065 0.34 -1.5 373,487 - EX31_RS06065 0.34 -0.3 373,563 - EX31_RS06065 0.42 -1.4 373,563 - EX31_RS06065 0.42 -1.1 373,563 - EX31_RS06065 0.42 -1.9 373,563 - EX31_RS06065 0.42 -1.6 373,563 - EX31_RS06065 0.42 -1.6 373,567 - EX31_RS06065 0.43 -1.9 373,580 - EX31_RS06065 0.44 -2.6 373,610 - EX31_RS06065 0.47 -0.9 373,668 - EX31_RS06065 0.53 -1.2 373,693 - EX31_RS06065 0.56 +0.2 373,745 - EX31_RS06065 0.61 +0.9 373,745 - EX31_RS06065 0.61 -1.4 373,868 - EX31_RS06065 0.74 -0.6 374,021 - -0.5 374,021 - -0.1 374,021 - -1.9 374,021 - -2.4 374,021 - +0.5 374,021 - -1.4 374,021 - -0.1 374,021 - -0.3 374,025 - -3.7 374,025 - -1.3 374,025 - -0.8 374,107 - +2.5 374,130 - -1.5 374,130 - -2.0 374,141 - +0.3 374,141 - -0.3 374,141 - +0.3 374,184 - -0.8 374,184 - -0.7 374,184 - -2.2 374,184 - +1.2 374,184 - +1.3 374,184 - -0.3 374,184 - -0.5 374,184 - -1.8 374,184 - -1.3 374,184 - -0.2 374,184 - -0.2 374,184 - -0.9 374,184 - +0.2 374,184 - +0.6 374,184 - +0.6 374,184 - +1.2 374,189 - +0.4 374,189 - -0.1 374,189 - -0.7 374,189 - +0.9 374,189 - +0.5 374,189 - +0.4 374,189 - -0.2 374,189 - +1.1 374,189 - -0.1 374,189 - +0.2 374,189 - -0.3 374,189 - +0.1 374,189 - -0.0 374,189 - +1.6 374,189 - -0.6 374,189 - +0.2 374,213 - +0.3 374,213 - -0.2 374,213 - +1.5 374,216 - +0.3 374,216 - +0.1 374,216 - -0.3 374,275 - +1.1 374,275 - +0.0 374,275 - -0.4 374,336 - EX31_RS06070 0.12 -3.0 374,336 - EX31_RS06070 0.12 -2.0 374,548 - EX31_RS06070 0.32 -1.2 374,561 - EX31_RS06070 0.34 -1.5 374,561 - EX31_RS06070 0.34 -1.1 374,561 - EX31_RS06070 0.34 -0.1 374,585 - EX31_RS06070 0.36 -1.3 374,591 - EX31_RS06070 0.37 -1.1 374,591 - EX31_RS06070 0.37 -3.2 374,591 - EX31_RS06070 0.37 -2.4 374,591 - EX31_RS06070 0.37 -1.1 374,591 - EX31_RS06070 0.37 -1.9 374,591 - EX31_RS06070 0.37 -2.3 374,591 - EX31_RS06070 0.37 -0.3 374,596 - EX31_RS06070 0.37 -2.3 374,596 - EX31_RS06070 0.37 -2.0 374,596 - EX31_RS06070 0.37 -0.4 374,596 - EX31_RS06070 0.37 -3.6 374,596 - EX31_RS06070 0.37 -3.3 374,596 - EX31_RS06070 0.37 -1.9 374,600 - EX31_RS06070 0.37 -1.0 374,679 - EX31_RS06070 0.45 -0.5 374,679 - EX31_RS06070 0.45 -0.8 374,685 - EX31_RS06070 0.46 -1.6 374,685 - EX31_RS06070 0.46 -0.3 374,685 - EX31_RS06070 0.46 -0.3 374,735 - EX31_RS06070 0.50 -1.5 374,757 - EX31_RS06070 0.52 -2.1 374,867 - EX31_RS06070 0.63 -2.8 374,867 - EX31_RS06070 0.63 -1.2 374,867 - EX31_RS06070 0.63 -1.4 374,867 - EX31_RS06070 0.63 -1.2 374,867 - EX31_RS06070 0.63 -2.0 374,871 - EX31_RS06070 0.63 -1.3 374,871 - EX31_RS06070 0.63 -1.5 374,871 - EX31_RS06070 0.63 -0.5 374,871 - EX31_RS06070 0.63 +1.5 374,871 - EX31_RS06070 0.63 -0.4 374,958 - EX31_RS06070 0.72 -0.5 375,079 + EX31_RS06070 0.83 +0.9 375,080 - EX31_RS06070 0.83 -2.1 375,126 - EX31_RS06070 0.88 -1.8 375,126 - EX31_RS06070 0.88 -1.2 375,126 - EX31_RS06070 0.88 -1.6 375,130 + EX31_RS06070 0.88 -1.2 375,131 - EX31_RS06070 0.88 -0.6 375,131 - EX31_RS06070 0.88 +0.2 375,217 - -0.7 375,217 - -1.7 375,217 - -1.6 375,237 - -2.2 375,237 - -1.2 375,297 - +0.3 375,300 + +0.5 375,300 + +2.5 375,300 + -1.1 375,300 + -1.0 375,301 - +1.2 375,301 - -0.2 375,301 - -0.3 375,301 - -0.5 375,325 + -2.6 375,326 - -1.3 375,326 - +0.1 375,326 - -0.6 375,326 - -1.4 375,335 + +0.5 375,336 - +0.8 375,336 - -1.1 375,344 + -1.8 375,351 + +0.0 375,351 + -0.3 375,352 - -0.2 375,352 - -0.3 375,352 - -0.4 375,356 + -0.3 375,356 + -1.2 375,356 + +0.1 375,357 - +0.1 375,358 + -0.3 375,358 + -2.3 375,358 + -0.3 375,358 + -1.0 375,359 - +0.1 375,359 - +0.2 375,359 - -0.6 375,417 + +0.6 375,417 + -1.2 375,435 + -0.1 375,436 - -0.2 375,436 - +1.2 375,438 + +0.2 375,438 + -1.0 375,439 - -1.1 375,439 - +0.4 375,439 - +0.3 375,439 - +0.1 375,470 - -0.7 375,470 - -0.1 375,470 - +1.2 375,642 - EX31_RS06075 0.20 -1.4 375,642 - EX31_RS06075 0.20 +1.9 375,693 + EX31_RS06075 0.26 -1.3 375,694 - EX31_RS06075 0.26 -1.2 375,717 - EX31_RS06075 0.29 +0.5 375,717 - EX31_RS06075 0.29 -1.6 375,738 - EX31_RS06075 0.31 +0.6 375,738 - EX31_RS06075 0.31 -0.6 375,738 - EX31_RS06075 0.31 +0.2 375,761 + EX31_RS06075 0.34 -0.1 375,761 + EX31_RS06075 0.34 +0.2 375,761 + EX31_RS06075 0.34 +0.3 375,762 - EX31_RS06075 0.34 +0.7 375,785 + EX31_RS06075 0.36 -1.1 375,786 - EX31_RS06075 0.36 -0.4 375,804 - EX31_RS06075 0.38 +0.2 375,894 - EX31_RS06075 0.49 -0.6 375,894 - EX31_RS06075 0.49 +0.1 375,929 + EX31_RS06075 0.53 +0.4 375,929 + EX31_RS06075 0.53 -0.2 375,929 + EX31_RS06075 0.53 +0.2 375,929 + EX31_RS06075 0.53 -0.4 375,929 + EX31_RS06075 0.53 -0.7 375,930 - EX31_RS06075 0.53 -1.4 375,930 - EX31_RS06075 0.53 -1.6 375,931 + EX31_RS06075 0.53 -1.2 375,931 + EX31_RS06075 0.53 +0.2 375,931 + EX31_RS06075 0.53 +0.7 375,932 - EX31_RS06075 0.53 -0.5 375,932 - EX31_RS06075 0.53 -0.2 375,932 - EX31_RS06075 0.53 -0.6 375,932 - EX31_RS06075 0.53 +0.0 375,932 - EX31_RS06075 0.53 -0.8 376,114 - EX31_RS06075 0.74 -0.3 376,133 + EX31_RS06075 0.76 +1.2 376,133 + EX31_RS06075 0.76 -0.1 376,134 - EX31_RS06075 0.76 +0.1 376,134 - EX31_RS06075 0.76 +0.1 376,134 - EX31_RS06075 0.76 -0.3 376,134 - EX31_RS06075 0.76 -0.5 376,167 - EX31_RS06075 0.80 -0.5 376,239 - EX31_RS06075 0.88 +1.3
Or see this region's nucleotide sequence