Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06045 and EX31_RS06050 are separated by 84 nucleotides EX31_RS06050 and EX31_RS06055 are separated by 10 nucleotides EX31_RS06055 and EX31_RS06060 are separated by 92 nucleotides EX31_RS06060 and EX31_RS06065 are separated by 1 nucleotides
EX31_RS06045: EX31_RS06045 - TetR family transcriptional regulator, at 369,892 to 370,584
_RS06045
EX31_RS06050: EX31_RS06050 - phosphate signaling complex protein PhoU, at 370,669 to 371,400
_RS06050
EX31_RS06055: EX31_RS06055 - phosphate ABC transporter ATP-binding protein PstB, at 371,411 to 372,178
_RS06055
EX31_RS06060: EX31_RS06060 - phosphate ABC transporter permease PstA, at 372,271 to 373,158
_RS06060
EX31_RS06065: EX31_RS06065 - phosphate ABC transporter permease PstC, at 373,160 to 374,116
_RS06065
Position (kb) 371
372
373 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 370.480 kb on + strand, within EX31_RS06045 at 370.481 kb on - strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.482 kb on + strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.483 kb on - strand, within EX31_RS06045 at 370.523 kb on + strand at 370.523 kb on + strand at 370.524 kb on - strand at 370.524 kb on - strand at 370.538 kb on - strand at 370.570 kb on + strand at 370.570 kb on + strand at 370.583 kb on - strand at 370.583 kb on - strand at 370.583 kb on - strand at 370.605 kb on + strand at 370.607 kb on + strand at 370.607 kb on + strand at 370.607 kb on + strand at 370.607 kb on + strand at 370.607 kb on + strand at 370.607 kb on + strand at 370.608 kb on - strand at 370.608 kb on - strand at 370.608 kb on - strand at 370.608 kb on - strand at 370.608 kb on - strand at 370.662 kb on + strand at 370.707 kb on + strand at 370.798 kb on - strand, within EX31_RS06050 at 370.869 kb on - strand, within EX31_RS06050 at 371.384 kb on - strand at 371.408 kb on - strand at 371.408 kb on - strand at 371.429 kb on - strand at 371.434 kb on - strand at 371.434 kb on - strand at 371.434 kb on - strand at 371.434 kb on - strand at 371.434 kb on - strand at 371.434 kb on - strand at 371.488 kb on - strand, within EX31_RS06055 at 371.509 kb on - strand, within EX31_RS06055 at 371.509 kb on - strand, within EX31_RS06055 at 371.509 kb on - strand, within EX31_RS06055 at 371.509 kb on - strand, within EX31_RS06055 at 371.509 kb on - strand, within EX31_RS06055 at 371.513 kb on - strand, within EX31_RS06055 at 371.696 kb on - strand, within EX31_RS06055 at 371.731 kb on - strand, within EX31_RS06055 at 371.731 kb on - strand, within EX31_RS06055 at 371.939 kb on - strand, within EX31_RS06055 at 371.939 kb on - strand, within EX31_RS06055 at 371.939 kb on - strand, within EX31_RS06055 at 371.939 kb on - strand, within EX31_RS06055 at 372.041 kb on - strand, within EX31_RS06055 at 372.130 kb on - strand at 372.224 kb on - strand at 372.334 kb on - strand at 372.344 kb on - strand at 372.344 kb on - strand at 372.344 kb on - strand at 372.397 kb on - strand, within EX31_RS06060 at 372.469 kb on - strand, within EX31_RS06060 at 372.469 kb on - strand, within EX31_RS06060 at 372.469 kb on - strand, within EX31_RS06060 at 372.499 kb on - strand, within EX31_RS06060 at 372.730 kb on - strand, within EX31_RS06060 at 372.730 kb on - strand, within EX31_RS06060 at 372.738 kb on - strand, within EX31_RS06060 at 372.820 kb on - strand, within EX31_RS06060 at 372.820 kb on - strand, within EX31_RS06060 at 372.820 kb on - strand, within EX31_RS06060 at 372.820 kb on - strand, within EX31_RS06060 at 372.840 kb on - strand, within EX31_RS06060 at 372.940 kb on - strand, within EX31_RS06060 at 372.997 kb on - strand, within EX31_RS06060 at 373.057 kb on - strand, within EX31_RS06060 at 373.124 kb on - strand at 373.135 kb on - strand at 373.135 kb on - strand at 373.135 kb on - strand at 373.135 kb on - strand at 373.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 370,480 + EX31_RS06045 0.85 +0.9 370,481 - EX31_RS06045 0.85 +0.9 370,482 + EX31_RS06045 0.85 +0.1 370,482 + EX31_RS06045 0.85 -0.3 370,482 + EX31_RS06045 0.85 -0.1 370,482 + EX31_RS06045 0.85 +0.7 370,482 + EX31_RS06045 0.85 +0.4 370,482 + EX31_RS06045 0.85 -0.6 370,482 + EX31_RS06045 0.85 +1.3 370,482 + EX31_RS06045 0.85 -0.9 370,482 + EX31_RS06045 0.85 +0.9 370,482 + EX31_RS06045 0.85 +0.7 370,482 + EX31_RS06045 0.85 +0.7 370,482 + EX31_RS06045 0.85 -0.2 370,482 + EX31_RS06045 0.85 +0.2 370,482 + EX31_RS06045 0.85 +0.4 370,483 - EX31_RS06045 0.85 +0.7 370,483 - EX31_RS06045 0.85 -0.6 370,483 - EX31_RS06045 0.85 +0.1 370,483 - EX31_RS06045 0.85 -0.5 370,483 - EX31_RS06045 0.85 -2.1 370,483 - EX31_RS06045 0.85 +0.5 370,483 - EX31_RS06045 0.85 +0.3 370,483 - EX31_RS06045 0.85 -0.9 370,483 - EX31_RS06045 0.85 +0.5 370,483 - EX31_RS06045 0.85 +0.1 370,483 - EX31_RS06045 0.85 +0.1 370,483 - EX31_RS06045 0.85 -0.2 370,483 - EX31_RS06045 0.85 -0.3 370,483 - EX31_RS06045 0.85 +2.1 370,483 - EX31_RS06045 0.85 -0.0 370,483 - EX31_RS06045 0.85 -0.9 370,483 - EX31_RS06045 0.85 -0.3 370,483 - EX31_RS06045 0.85 +0.1 370,483 - EX31_RS06045 0.85 +0.1 370,483 - EX31_RS06045 0.85 -1.1 370,483 - EX31_RS06045 0.85 -0.2 370,523 + +0.1 370,523 + +0.0 370,524 - +0.5 370,524 - -2.4 370,538 - +0.3 370,570 + -0.3 370,570 + +0.3 370,583 - -0.6 370,583 - -0.1 370,583 - -0.3 370,605 + +1.1 370,607 + -2.3 370,607 + +0.1 370,607 + +0.2 370,607 + +0.1 370,607 + +0.0 370,607 + -1.5 370,608 - -0.5 370,608 - -1.1 370,608 - +0.2 370,608 - +0.5 370,608 - -0.3 370,662 + +1.2 370,707 + +0.2 370,798 - EX31_RS06050 0.18 -0.6 370,869 - EX31_RS06050 0.27 +0.2 371,384 - -1.2 371,408 - +0.5 371,408 - -0.7 371,429 - -1.8 371,434 - +0.1 371,434 - -2.8 371,434 - -1.2 371,434 - -1.7 371,434 - -0.5 371,434 - -0.8 371,488 - EX31_RS06055 0.10 -1.1 371,509 - EX31_RS06055 0.13 -1.4 371,509 - EX31_RS06055 0.13 +1.5 371,509 - EX31_RS06055 0.13 -0.3 371,509 - EX31_RS06055 0.13 -0.6 371,509 - EX31_RS06055 0.13 -1.4 371,513 - EX31_RS06055 0.13 -0.3 371,696 - EX31_RS06055 0.37 -1.5 371,731 - EX31_RS06055 0.42 -1.4 371,731 - EX31_RS06055 0.42 -1.7 371,939 - EX31_RS06055 0.69 -2.3 371,939 - EX31_RS06055 0.69 -1.7 371,939 - EX31_RS06055 0.69 +0.9 371,939 - EX31_RS06055 0.69 -0.7 372,041 - EX31_RS06055 0.82 -0.5 372,130 - -3.4 372,224 - -0.4 372,334 - +0.1 372,344 - +2.5 372,344 - +0.9 372,344 - +1.7 372,397 - EX31_RS06060 0.14 -0.6 372,469 - EX31_RS06060 0.22 -0.1 372,469 - EX31_RS06060 0.22 -1.4 372,469 - EX31_RS06060 0.22 -1.2 372,499 - EX31_RS06060 0.26 -1.1 372,730 - EX31_RS06060 0.52 -0.3 372,730 - EX31_RS06060 0.52 -1.2 372,738 - EX31_RS06060 0.53 -2.3 372,820 - EX31_RS06060 0.62 -0.6 372,820 - EX31_RS06060 0.62 -0.6 372,820 - EX31_RS06060 0.62 -2.2 372,820 - EX31_RS06060 0.62 -1.5 372,840 - EX31_RS06060 0.64 +0.1 372,940 - EX31_RS06060 0.75 -1.7 372,997 - EX31_RS06060 0.82 -1.6 373,057 - EX31_RS06060 0.89 -1.9 373,124 - -0.8 373,135 - -0.1 373,135 - -1.4 373,135 - -0.2 373,135 - -0.6 373,135 - -2.3
Or see this region's nucleotide sequence