Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS04720 and EX31_RS04725 are separated by 57 nucleotides EX31_RS04725 and EX31_RS04730 are separated by 348 nucleotides
EX31_RS04720: EX31_RS04720 - 3-dehydroquinate synthase, at 60,154 to 61,236
_RS04720
EX31_RS04725: EX31_RS04725 - shikimate kinase AroK, at 61,294 to 61,815
_RS04725
EX31_RS04730: EX31_RS04730 - DNA uptake porin HofQ, at 62,164 to 63,426
_RS04730
Position (kb) 61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 61.259 kb on - strand at 61.292 kb on - strand at 61.292 kb on - strand at 61.292 kb on - strand at 61.300 kb on - strand at 61.300 kb on - strand at 61.312 kb on - strand at 61.312 kb on - strand at 61.312 kb on - strand at 61.312 kb on - strand at 61.312 kb on - strand at 61.363 kb on - strand, within EX31_RS04725 at 61.386 kb on - strand, within EX31_RS04725 at 61.386 kb on - strand, within EX31_RS04725 at 61.448 kb on - strand, within EX31_RS04725 at 61.448 kb on - strand, within EX31_RS04725 at 61.448 kb on - strand, within EX31_RS04725 at 61.448 kb on - strand, within EX31_RS04725 at 61.448 kb on - strand, within EX31_RS04725 at 61.451 kb on - strand, within EX31_RS04725 at 61.501 kb on - strand, within EX31_RS04725 at 61.501 kb on - strand, within EX31_RS04725 at 61.667 kb on - strand, within EX31_RS04725 at 61.667 kb on - strand, within EX31_RS04725 at 61.745 kb on - strand, within EX31_RS04725 at 61.745 kb on - strand, within EX31_RS04725 at 61.745 kb on - strand, within EX31_RS04725 at 61.745 kb on - strand, within EX31_RS04725 at 61.798 kb on - strand at 61.798 kb on - strand at 61.798 kb on - strand at 61.829 kb on - strand at 61.829 kb on - strand at 61.829 kb on - strand at 61.832 kb on - strand at 61.832 kb on - strand at 61.832 kb on - strand at 61.832 kb on - strand at 61.851 kb on - strand at 61.886 kb on - strand at 61.908 kb on - strand at 61.908 kb on - strand at 61.908 kb on - strand at 61.908 kb on - strand at 61.908 kb on - strand at 61.909 kb on + strand at 61.910 kb on - strand at 61.910 kb on - strand at 61.910 kb on - strand at 61.910 kb on - strand at 61.910 kb on - strand at 61.910 kb on - strand at 61.910 kb on - strand at 61.910 kb on - strand at 61.932 kb on - strand at 61.948 kb on + strand at 61.949 kb on - strand at 61.949 kb on - strand at 61.949 kb on - strand at 61.949 kb on - strand at 61.965 kb on - strand at 61.998 kb on - strand at 62.023 kb on - strand at 62.026 kb on - strand at 62.060 kb on + strand at 62.060 kb on + strand at 62.060 kb on + strand at 62.060 kb on + strand at 62.060 kb on + strand at 62.060 kb on + strand at 62.060 kb on + strand at 62.060 kb on + strand at 62.061 kb on - strand at 62.061 kb on - strand at 62.061 kb on - strand at 62.065 kb on + strand at 62.065 kb on + strand at 62.065 kb on + strand at 62.066 kb on - strand at 62.066 kb on - strand at 62.130 kb on + strand at 62.131 kb on - strand at 62.133 kb on + strand at 62.133 kb on + strand at 62.133 kb on + strand at 62.133 kb on + strand at 62.133 kb on + strand at 62.133 kb on + strand at 62.133 kb on + strand at 62.133 kb on + strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.134 kb on - strand at 62.142 kb on + strand at 62.142 kb on + strand at 62.142 kb on + strand at 62.142 kb on + strand at 62.142 kb on + strand at 62.142 kb on + strand at 62.142 kb on + strand at 62.142 kb on + strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.143 kb on - strand at 62.165 kb on + strand at 62.179 kb on + strand at 62.180 kb on - strand at 62.228 kb on + strand at 62.228 kb on + strand at 62.228 kb on + strand at 62.228 kb on + strand at 62.228 kb on + strand at 62.228 kb on + strand at 62.229 kb on - strand at 62.229 kb on - strand at 62.229 kb on - strand at 62.229 kb on - strand at 62.229 kb on - strand at 62.229 kb on - strand at 62.229 kb on - strand at 62.287 kb on - strand at 62.304 kb on + strand, within EX31_RS04730 at 62.315 kb on - strand, within EX31_RS04730 at 62.326 kb on + strand, within EX31_RS04730 at 62.326 kb on + strand, within EX31_RS04730 at 62.366 kb on - strand, within EX31_RS04730 at 62.366 kb on - strand, within EX31_RS04730 at 62.366 kb on - strand, within EX31_RS04730 at 62.377 kb on - strand, within EX31_RS04730 at 62.377 kb on - strand, within EX31_RS04730 at 62.412 kb on + strand, within EX31_RS04730 at 62.412 kb on + strand, within EX31_RS04730 at 62.412 kb on + strand, within EX31_RS04730 at 62.413 kb on - strand, within EX31_RS04730 at 62.458 kb on - strand, within EX31_RS04730 at 62.577 kb on + strand, within EX31_RS04730 at 62.578 kb on - strand, within EX31_RS04730 at 62.578 kb on - strand, within EX31_RS04730 at 62.580 kb on + strand, within EX31_RS04730 at 62.580 kb on + strand, within EX31_RS04730 at 62.580 kb on + strand, within EX31_RS04730 at 62.581 kb on - strand, within EX31_RS04730 at 62.595 kb on + strand, within EX31_RS04730 at 62.595 kb on + strand, within EX31_RS04730 at 62.595 kb on + strand, within EX31_RS04730 at 62.596 kb on - strand, within EX31_RS04730 at 62.596 kb on - strand, within EX31_RS04730 at 62.626 kb on - strand, within EX31_RS04730 at 62.628 kb on + strand, within EX31_RS04730 at 62.628 kb on + strand, within EX31_RS04730 at 62.628 kb on + strand, within EX31_RS04730 at 62.637 kb on + strand, within EX31_RS04730 at 62.637 kb on + strand, within EX31_RS04730 at 62.637 kb on + strand, within EX31_RS04730 at 62.638 kb on - strand, within EX31_RS04730 at 62.638 kb on - strand, within EX31_RS04730 at 62.712 kb on + strand, within EX31_RS04730 at 62.712 kb on + strand, within EX31_RS04730 at 62.712 kb on + strand, within EX31_RS04730 at 62.713 kb on - strand, within EX31_RS04730 at 62.713 kb on - strand, within EX31_RS04730 at 62.716 kb on + strand, within EX31_RS04730 at 62.717 kb on - strand, within EX31_RS04730 at 62.772 kb on + strand, within EX31_RS04730 at 62.773 kb on - strand, within EX31_RS04730 at 62.773 kb on - strand, within EX31_RS04730
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 61,259 - +0.6 61,292 - +1.3 61,292 - -0.5 61,292 - -2.0 61,300 - -1.0 61,300 - -2.3 61,312 - -1.6 61,312 - -1.6 61,312 - +0.2 61,312 - -0.8 61,312 - -1.0 61,363 - EX31_RS04725 0.13 -1.5 61,386 - EX31_RS04725 0.18 -0.5 61,386 - EX31_RS04725 0.18 -0.1 61,448 - EX31_RS04725 0.30 -1.5 61,448 - EX31_RS04725 0.30 +0.2 61,448 - EX31_RS04725 0.30 -0.7 61,448 - EX31_RS04725 0.30 -0.8 61,448 - EX31_RS04725 0.30 -0.1 61,451 - EX31_RS04725 0.30 -1.1 61,501 - EX31_RS04725 0.40 -1.3 61,501 - EX31_RS04725 0.40 -1.0 61,667 - EX31_RS04725 0.71 +0.7 61,667 - EX31_RS04725 0.71 -0.8 61,745 - EX31_RS04725 0.86 +0.3 61,745 - EX31_RS04725 0.86 -2.3 61,745 - EX31_RS04725 0.86 -3.1 61,745 - EX31_RS04725 0.86 +0.2 61,798 - -1.7 61,798 - -1.3 61,798 - +0.2 61,829 - +1.9 61,829 - -0.9 61,829 - +0.5 61,832 - -1.5 61,832 - -0.4 61,832 - -2.1 61,832 - -1.1 61,851 - -0.1 61,886 - +0.3 61,908 - -1.0 61,908 - -0.6 61,908 - -0.4 61,908 - -1.5 61,908 - +0.1 61,909 + -0.1 61,910 - +0.3 61,910 - +0.3 61,910 - -0.9 61,910 - +0.0 61,910 - +0.1 61,910 - -1.0 61,910 - +1.0 61,910 - -0.2 61,932 - -1.0 61,948 + -1.0 61,949 - +0.4 61,949 - -0.7 61,949 - -1.0 61,949 - +0.4 61,965 - -1.1 61,998 - -0.1 62,023 - +0.2 62,026 - +0.6 62,060 + -0.1 62,060 + +0.6 62,060 + -0.0 62,060 + -0.1 62,060 + +0.0 62,060 + -0.8 62,060 + -1.1 62,060 + -0.0 62,061 - +0.3 62,061 - -0.8 62,061 - -0.3 62,065 + -2.5 62,065 + +1.8 62,065 + -0.0 62,066 - +0.5 62,066 - +0.4 62,130 + +0.8 62,131 - +0.6 62,133 + +0.7 62,133 + +0.5 62,133 + +0.4 62,133 + +0.0 62,133 + +1.1 62,133 + -0.7 62,133 + +0.3 62,133 + -1.5 62,134 - -0.3 62,134 - +0.2 62,134 - -0.6 62,134 - +1.0 62,134 - -0.6 62,134 - -2.3 62,134 - -0.3 62,134 - +0.5 62,134 - -0.9 62,134 - +0.3 62,134 - -0.8 62,142 + -0.0 62,142 + -0.5 62,142 + +0.6 62,142 + +0.4 62,142 + +0.4 62,142 + -1.3 62,142 + -0.2 62,142 + -0.2 62,143 - -0.1 62,143 - -0.1 62,143 - +1.0 62,143 - +0.1 62,143 - -1.2 62,143 - -0.7 62,143 - -0.8 62,143 - -0.2 62,143 - -0.0 62,165 + +0.3 62,179 + -0.1 62,180 - -0.9 62,228 + +0.5 62,228 + -1.3 62,228 + +0.1 62,228 + -1.8 62,228 + +0.1 62,228 + -1.5 62,229 - +1.0 62,229 - -1.1 62,229 - -1.1 62,229 - -0.2 62,229 - +0.0 62,229 - -0.3 62,229 - +2.5 62,287 - +0.2 62,304 + EX31_RS04730 0.11 -0.3 62,315 - EX31_RS04730 0.12 +0.4 62,326 + EX31_RS04730 0.13 -0.5 62,326 + EX31_RS04730 0.13 +1.0 62,366 - EX31_RS04730 0.16 -1.5 62,366 - EX31_RS04730 0.16 +0.5 62,366 - EX31_RS04730 0.16 -0.7 62,377 - EX31_RS04730 0.17 +0.2 62,377 - EX31_RS04730 0.17 +0.3 62,412 + EX31_RS04730 0.20 +0.2 62,412 + EX31_RS04730 0.20 -0.6 62,412 + EX31_RS04730 0.20 -0.4 62,413 - EX31_RS04730 0.20 +0.8 62,458 - EX31_RS04730 0.23 -0.2 62,577 + EX31_RS04730 0.33 -0.2 62,578 - EX31_RS04730 0.33 +0.8 62,578 - EX31_RS04730 0.33 -0.2 62,580 + EX31_RS04730 0.33 +0.9 62,580 + EX31_RS04730 0.33 +0.1 62,580 + EX31_RS04730 0.33 +0.4 62,581 - EX31_RS04730 0.33 +0.8 62,595 + EX31_RS04730 0.34 +0.1 62,595 + EX31_RS04730 0.34 +0.2 62,595 + EX31_RS04730 0.34 -0.1 62,596 - EX31_RS04730 0.34 -0.8 62,596 - EX31_RS04730 0.34 -0.1 62,626 - EX31_RS04730 0.37 +0.2 62,628 + EX31_RS04730 0.37 +0.5 62,628 + EX31_RS04730 0.37 -0.3 62,628 + EX31_RS04730 0.37 -1.1 62,637 + EX31_RS04730 0.37 -0.5 62,637 + EX31_RS04730 0.37 +1.2 62,637 + EX31_RS04730 0.37 -0.6 62,638 - EX31_RS04730 0.38 -1.3 62,638 - EX31_RS04730 0.38 +0.0 62,712 + EX31_RS04730 0.43 +0.2 62,712 + EX31_RS04730 0.43 +1.6 62,712 + EX31_RS04730 0.43 +0.1 62,713 - EX31_RS04730 0.43 +0.5 62,713 - EX31_RS04730 0.43 +0.0 62,716 + EX31_RS04730 0.44 -0.4 62,717 - EX31_RS04730 0.44 +0.3 62,772 + EX31_RS04730 0.48 -1.0 62,773 - EX31_RS04730 0.48 +0.3 62,773 - EX31_RS04730 0.48 -0.3
Or see this region's nucleotide sequence