Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02400 and EX31_RS02405 are separated by 195 nucleotides EX31_RS02405 and EX31_RS02410 are separated by 319 nucleotides EX31_RS02410 and EX31_RS02415 are separated by 285 nucleotides
EX31_RS02400: EX31_RS02400 - MetQ/NlpA family lipoprotein, at 510,265 to 511,077
_RS02400
EX31_RS02405: EX31_RS02405 - MFS transporter, at 511,273 to 512,484
_RS02405
EX31_RS02410: EX31_RS02410 - multidrug DMT transporter, at 512,804 to 513,139
_RS02410
EX31_RS02415: EX31_RS02415 - hypothetical protein, at 513,425 to 513,727
_RS02415
Position (kb) 511
512
513 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 510.293 kb on + strand at 510.293 kb on + strand at 510.294 kb on - strand at 510.294 kb on - strand at 510.298 kb on - strand at 510.298 kb on - strand at 510.373 kb on - strand, within EX31_RS02400 at 510.393 kb on + strand, within EX31_RS02400 at 510.394 kb on - strand, within EX31_RS02400 at 510.401 kb on + strand, within EX31_RS02400 at 510.402 kb on - strand, within EX31_RS02400 at 510.414 kb on + strand, within EX31_RS02400 at 510.468 kb on - strand, within EX31_RS02400 at 510.468 kb on - strand, within EX31_RS02400 at 510.475 kb on - strand, within EX31_RS02400 at 510.599 kb on - strand, within EX31_RS02400 at 510.719 kb on - strand, within EX31_RS02400 at 510.909 kb on + strand, within EX31_RS02400 at 510.909 kb on + strand, within EX31_RS02400 at 510.911 kb on + strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.912 kb on - strand, within EX31_RS02400 at 510.981 kb on + strand, within EX31_RS02400 at 510.981 kb on + strand, within EX31_RS02400 at 510.982 kb on - strand, within EX31_RS02400 at 511.043 kb on - strand at 511.167 kb on + strand at 511.186 kb on - strand at 511.186 kb on - strand at 511.215 kb on - strand at 511.265 kb on + strand at 511.266 kb on - strand at 511.291 kb on + strand at 511.292 kb on - strand at 511.621 kb on - strand, within EX31_RS02405 at 511.629 kb on + strand, within EX31_RS02405 at 511.662 kb on + strand, within EX31_RS02405 at 511.662 kb on + strand, within EX31_RS02405 at 511.663 kb on - strand, within EX31_RS02405 at 511.685 kb on - strand, within EX31_RS02405 at 511.688 kb on - strand, within EX31_RS02405 at 511.725 kb on + strand, within EX31_RS02405 at 511.772 kb on + strand, within EX31_RS02405 at 511.773 kb on - strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.776 kb on + strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.777 kb on - strand, within EX31_RS02405 at 511.778 kb on + strand, within EX31_RS02405 at 511.778 kb on + strand, within EX31_RS02405 at 511.778 kb on + strand, within EX31_RS02405 at 511.804 kb on - strand, within EX31_RS02405 at 511.807 kb on + strand, within EX31_RS02405 at 511.808 kb on - strand, within EX31_RS02405 at 511.948 kb on - strand, within EX31_RS02405 at 511.953 kb on + strand, within EX31_RS02405 at 511.954 kb on - strand, within EX31_RS02405 at 511.954 kb on - strand, within EX31_RS02405 at 511.954 kb on - strand, within EX31_RS02405 at 511.987 kb on - strand, within EX31_RS02405 at 512.138 kb on - strand, within EX31_RS02405 at 512.221 kb on + strand, within EX31_RS02405 at 512.221 kb on + strand, within EX31_RS02405 at 512.221 kb on + strand, within EX31_RS02405 at 512.222 kb on - strand, within EX31_RS02405 at 512.222 kb on - strand, within EX31_RS02405 at 512.224 kb on + strand, within EX31_RS02405 at 512.224 kb on + strand, within EX31_RS02405 at 512.224 kb on + strand, within EX31_RS02405 at 512.225 kb on - strand, within EX31_RS02405 at 512.225 kb on - strand, within EX31_RS02405 at 512.225 kb on - strand, within EX31_RS02405 at 512.225 kb on - strand, within EX31_RS02405 at 512.287 kb on + strand, within EX31_RS02405 at 512.287 kb on + strand, within EX31_RS02405 at 512.288 kb on - strand, within EX31_RS02405 at 512.385 kb on + strand at 512.532 kb on - strand at 512.597 kb on - strand at 512.604 kb on - strand at 512.619 kb on - strand at 512.619 kb on - strand at 512.683 kb on + strand at 512.683 kb on + strand at 512.684 kb on - strand at 512.684 kb on - strand at 512.684 kb on - strand at 512.689 kb on + strand at 512.690 kb on - strand at 512.717 kb on + strand at 512.728 kb on + strand at 512.821 kb on + strand at 512.821 kb on + strand at 512.822 kb on - strand at 512.827 kb on - strand at 512.827 kb on - strand at 512.842 kb on + strand, within EX31_RS02410 at 512.843 kb on - strand, within EX31_RS02410 at 512.843 kb on - strand, within EX31_RS02410 at 512.901 kb on + strand, within EX31_RS02410 at 512.901 kb on + strand, within EX31_RS02410 at 513.128 kb on + strand at 513.140 kb on - strand at 513.140 kb on - strand at 513.140 kb on - strand at 513.140 kb on - strand at 513.140 kb on - strand at 513.140 kb on - strand at 513.140 kb on - strand at 513.162 kb on + strand at 513.193 kb on + strand at 513.199 kb on - strand at 513.285 kb on - strand at 513.344 kb on - strand at 513.388 kb on - strand at 513.430 kb on + strand at 513.455 kb on - strand at 513.457 kb on - strand, within EX31_RS02415 at 513.457 kb on - strand, within EX31_RS02415
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 510,293 + -0.9 510,293 + -0.0 510,294 - +0.9 510,294 - -0.4 510,298 - +0.3 510,298 - -0.1 510,373 - EX31_RS02400 0.13 +0.1 510,393 + EX31_RS02400 0.16 +1.0 510,394 - EX31_RS02400 0.16 +0.7 510,401 + EX31_RS02400 0.17 +0.5 510,402 - EX31_RS02400 0.17 -0.0 510,414 + EX31_RS02400 0.18 +0.3 510,468 - EX31_RS02400 0.25 -0.5 510,468 - EX31_RS02400 0.25 -1.3 510,475 - EX31_RS02400 0.26 +1.3 510,599 - EX31_RS02400 0.41 +0.3 510,719 - EX31_RS02400 0.56 +1.2 510,909 + EX31_RS02400 0.79 -0.5 510,909 + EX31_RS02400 0.79 -1.9 510,911 + EX31_RS02400 0.79 -0.2 510,912 - EX31_RS02400 0.80 -0.3 510,912 - EX31_RS02400 0.80 +0.3 510,912 - EX31_RS02400 0.80 -0.1 510,912 - EX31_RS02400 0.80 +0.4 510,912 - EX31_RS02400 0.80 +0.9 510,912 - EX31_RS02400 0.80 -1.0 510,981 + EX31_RS02400 0.88 -0.2 510,981 + EX31_RS02400 0.88 +0.6 510,982 - EX31_RS02400 0.88 +0.7 511,043 - +2.3 511,167 + +0.3 511,186 - +0.4 511,186 - -0.2 511,215 - +1.5 511,265 + +0.7 511,266 - -0.0 511,291 + -3.1 511,292 - -0.9 511,621 - EX31_RS02405 0.29 -0.1 511,629 + EX31_RS02405 0.29 -1.2 511,662 + EX31_RS02405 0.32 -0.0 511,662 + EX31_RS02405 0.32 +1.2 511,663 - EX31_RS02405 0.32 +2.3 511,685 - EX31_RS02405 0.34 -0.6 511,688 - EX31_RS02405 0.34 +1.0 511,725 + EX31_RS02405 0.37 +0.3 511,772 + EX31_RS02405 0.41 -1.5 511,773 - EX31_RS02405 0.41 -1.7 511,776 + EX31_RS02405 0.42 +1.1 511,776 + EX31_RS02405 0.42 +0.7 511,776 + EX31_RS02405 0.42 +1.0 511,776 + EX31_RS02405 0.42 +1.8 511,776 + EX31_RS02405 0.42 +0.5 511,776 + EX31_RS02405 0.42 +0.6 511,776 + EX31_RS02405 0.42 -1.8 511,776 + EX31_RS02405 0.42 +2.3 511,776 + EX31_RS02405 0.42 -0.9 511,776 + EX31_RS02405 0.42 -1.7 511,776 + EX31_RS02405 0.42 +0.4 511,776 + EX31_RS02405 0.42 +0.5 511,776 + EX31_RS02405 0.42 +0.5 511,776 + EX31_RS02405 0.42 -0.0 511,776 + EX31_RS02405 0.42 -0.0 511,777 - EX31_RS02405 0.42 +1.7 511,777 - EX31_RS02405 0.42 +0.6 511,777 - EX31_RS02405 0.42 -1.5 511,777 - EX31_RS02405 0.42 +1.0 511,777 - EX31_RS02405 0.42 +1.1 511,777 - EX31_RS02405 0.42 +1.5 511,777 - EX31_RS02405 0.42 -0.6 511,777 - EX31_RS02405 0.42 +1.0 511,777 - EX31_RS02405 0.42 +1.3 511,778 + EX31_RS02405 0.42 -1.6 511,778 + EX31_RS02405 0.42 -0.3 511,778 + EX31_RS02405 0.42 +0.2 511,804 - EX31_RS02405 0.44 +2.9 511,807 + EX31_RS02405 0.44 +0.3 511,808 - EX31_RS02405 0.44 +1.1 511,948 - EX31_RS02405 0.56 +0.7 511,953 + EX31_RS02405 0.56 +0.4 511,954 - EX31_RS02405 0.56 +0.6 511,954 - EX31_RS02405 0.56 -0.6 511,954 - EX31_RS02405 0.56 +0.1 511,987 - EX31_RS02405 0.59 -0.8 512,138 - EX31_RS02405 0.71 -0.1 512,221 + EX31_RS02405 0.78 -0.2 512,221 + EX31_RS02405 0.78 -1.9 512,221 + EX31_RS02405 0.78 +0.5 512,222 - EX31_RS02405 0.78 +0.4 512,222 - EX31_RS02405 0.78 +0.7 512,224 + EX31_RS02405 0.78 +0.4 512,224 + EX31_RS02405 0.78 +1.1 512,224 + EX31_RS02405 0.78 -0.9 512,225 - EX31_RS02405 0.79 +1.1 512,225 - EX31_RS02405 0.79 +0.2 512,225 - EX31_RS02405 0.79 -0.6 512,225 - EX31_RS02405 0.79 +0.7 512,287 + EX31_RS02405 0.84 -0.3 512,287 + EX31_RS02405 0.84 +0.6 512,288 - EX31_RS02405 0.84 -0.7 512,385 + +2.3 512,532 - +2.3 512,597 - +1.1 512,604 - +0.7 512,619 - -0.7 512,619 - +1.0 512,683 + +0.2 512,683 + +0.7 512,684 - +0.3 512,684 - +1.1 512,684 - -0.3 512,689 + -0.3 512,690 - +0.7 512,717 + -0.4 512,728 + +2.0 512,821 + -1.6 512,821 + -0.7 512,822 - -0.2 512,827 - -0.5 512,827 - -0.7 512,842 + EX31_RS02410 0.11 +0.3 512,843 - EX31_RS02410 0.12 +1.0 512,843 - EX31_RS02410 0.12 +2.7 512,901 + EX31_RS02410 0.29 -0.3 512,901 + EX31_RS02410 0.29 +0.3 513,128 + +0.2 513,140 - +1.3 513,140 - +0.3 513,140 - -0.6 513,140 - -1.2 513,140 - -0.3 513,140 - +2.7 513,140 - +2.9 513,162 + -0.4 513,193 + -0.5 513,199 - +1.8 513,285 - -0.7 513,344 - -0.6 513,388 - +0.4 513,430 + +1.0 513,455 - -0.0 513,457 - EX31_RS02415 0.11 +3.3 513,457 - EX31_RS02415 0.11 -0.5
Or see this region's nucleotide sequence