Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02265 and EX31_RS02270 are separated by 60 nucleotides EX31_RS02270 and EX31_RS02275 are separated by 14 nucleotides EX31_RS02275 and EX31_RS02280 are separated by 65 nucleotides
EX31_RS02265: EX31_RS02265 - 6-phospho-beta-glucosidase, at 489,556 to 490,899
_RS02265
EX31_RS02270: EX31_RS02270 - transcriptional regulator ChbR, at 490,960 to 491,796
_RS02270
EX31_RS02275: EX31_RS02275 - PTS N,N'-diacetylchitobiose transporter subunit IIA, at 491,811 to 492,158
_RS02275
EX31_RS02280: EX31_RS02280 - PTS N,N'-diacetylchitobiose transporter subunit IIC, at 492,224 to 493,582
_RS02280
Position (kb) 490
491
492 Strain fitness (log2 ratio) -1
0
1
2 at 490.043 kb on - strand, within EX31_RS02265 at 490.071 kb on - strand, within EX31_RS02265 at 490.110 kb on + strand, within EX31_RS02265 at 490.115 kb on + strand, within EX31_RS02265 at 490.115 kb on + strand, within EX31_RS02265 at 490.118 kb on + strand, within EX31_RS02265 at 490.127 kb on + strand, within EX31_RS02265 at 490.178 kb on - strand, within EX31_RS02265 at 490.178 kb on - strand, within EX31_RS02265 at 490.278 kb on + strand, within EX31_RS02265 at 490.279 kb on - strand, within EX31_RS02265 at 490.461 kb on + strand, within EX31_RS02265 at 490.462 kb on - strand, within EX31_RS02265 at 490.527 kb on + strand, within EX31_RS02265 at 490.596 kb on + strand, within EX31_RS02265 at 490.700 kb on - strand, within EX31_RS02265 at 490.728 kb on + strand, within EX31_RS02265 at 490.750 kb on - strand, within EX31_RS02265 at 490.793 kb on - strand at 490.804 kb on - strand at 490.804 kb on - strand at 490.852 kb on - strand at 490.858 kb on - strand at 490.900 kb on - strand at 490.917 kb on - strand at 491.218 kb on - strand, within EX31_RS02270 at 491.413 kb on + strand, within EX31_RS02270 at 491.486 kb on + strand, within EX31_RS02270 at 491.486 kb on + strand, within EX31_RS02270 at 491.486 kb on + strand, within EX31_RS02270 at 491.487 kb on - strand, within EX31_RS02270 at 491.487 kb on - strand, within EX31_RS02270 at 491.524 kb on + strand, within EX31_RS02270 at 491.524 kb on + strand, within EX31_RS02270 at 491.670 kb on - strand, within EX31_RS02270 at 491.701 kb on + strand, within EX31_RS02270 at 492.147 kb on + strand at 492.274 kb on + strand at 492.275 kb on - strand at 492.292 kb on + strand at 492.292 kb on + strand at 492.292 kb on + strand at 492.293 kb on - strand at 492.293 kb on - strand at 492.315 kb on + strand at 492.315 kb on + strand at 492.316 kb on - strand at 492.316 kb on - strand at 492.316 kb on - strand at 492.316 kb on - strand at 492.509 kb on - strand, within EX31_RS02280 at 492.551 kb on - strand, within EX31_RS02280 at 492.572 kb on - strand, within EX31_RS02280 at 492.628 kb on + strand, within EX31_RS02280 at 492.681 kb on + strand, within EX31_RS02280 at 492.762 kb on + strand, within EX31_RS02280
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 490,043 - EX31_RS02265 0.36 +1.3 490,071 - EX31_RS02265 0.38 -0.8 490,110 + EX31_RS02265 0.41 -0.9 490,115 + EX31_RS02265 0.42 +1.1 490,115 + EX31_RS02265 0.42 -0.0 490,118 + EX31_RS02265 0.42 -0.1 490,127 + EX31_RS02265 0.42 +0.6 490,178 - EX31_RS02265 0.46 +1.8 490,178 - EX31_RS02265 0.46 +1.3 490,278 + EX31_RS02265 0.54 -0.5 490,279 - EX31_RS02265 0.54 +0.5 490,461 + EX31_RS02265 0.67 -1.5 490,462 - EX31_RS02265 0.67 -0.6 490,527 + EX31_RS02265 0.72 +0.3 490,596 + EX31_RS02265 0.77 -0.2 490,700 - EX31_RS02265 0.85 +0.9 490,728 + EX31_RS02265 0.87 +0.3 490,750 - EX31_RS02265 0.89 +0.2 490,793 - -0.9 490,804 - -0.3 490,804 - -0.4 490,852 - -0.4 490,858 - +0.7 490,900 - -0.5 490,917 - +0.2 491,218 - EX31_RS02270 0.31 +0.8 491,413 + EX31_RS02270 0.54 -0.5 491,486 + EX31_RS02270 0.63 +1.3 491,486 + EX31_RS02270 0.63 -0.0 491,486 + EX31_RS02270 0.63 -0.9 491,487 - EX31_RS02270 0.63 -0.5 491,487 - EX31_RS02270 0.63 +0.7 491,524 + EX31_RS02270 0.67 +0.3 491,524 + EX31_RS02270 0.67 +0.3 491,670 - EX31_RS02270 0.85 -0.0 491,701 + EX31_RS02270 0.89 +0.4 492,147 + +0.1 492,274 + -0.0 492,275 - +1.3 492,292 + -0.3 492,292 + +1.3 492,292 + -0.6 492,293 - +0.7 492,293 - -0.1 492,315 + +0.1 492,315 + -0.2 492,316 - -1.2 492,316 - +1.5 492,316 - +0.2 492,316 - -0.8 492,509 - EX31_RS02280 0.21 -0.1 492,551 - EX31_RS02280 0.24 -0.3 492,572 - EX31_RS02280 0.26 +0.1 492,628 + EX31_RS02280 0.30 +0.4 492,681 + EX31_RS02280 0.34 +0.1 492,762 + EX31_RS02280 0.40 +0.6
Or see this region's nucleotide sequence