Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01455 and EX31_RS01460 are separated by 122 nucleotides EX31_RS01460 and EX31_RS01465 are separated by 292 nucleotides
EX31_RS01455: EX31_RS01455 - alpha-amylase, at 327,093 to 328,574
_RS01455
EX31_RS01460: EX31_RS01460 - glycine betaine/L-proline transporter ProP, at 328,697 to 330,199
_RS01460
EX31_RS01465: EX31_RS01465 - oligogalacturonate lyase, at 330,492 to 331,673
_RS01465
Position (kb)
328
329
330
331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 327.746 kb on - strand, within EX31_RS01455 at 327.746 kb on - strand, within EX31_RS01455 at 327.746 kb on - strand, within EX31_RS01455 at 327.778 kb on + strand, within EX31_RS01455 at 327.793 kb on + strand, within EX31_RS01455 at 327.793 kb on + strand, within EX31_RS01455 at 327.794 kb on - strand, within EX31_RS01455 at 327.821 kb on + strand, within EX31_RS01455 at 327.822 kb on - strand, within EX31_RS01455 at 327.923 kb on - strand, within EX31_RS01455 at 327.985 kb on + strand, within EX31_RS01455 at 328.259 kb on + strand, within EX31_RS01455 at 328.260 kb on - strand, within EX31_RS01455 at 328.295 kb on + strand, within EX31_RS01455 at 328.296 kb on - strand, within EX31_RS01455 at 328.326 kb on + strand, within EX31_RS01455 at 328.327 kb on - strand, within EX31_RS01455 at 328.400 kb on - strand, within EX31_RS01455 at 328.400 kb on - strand, within EX31_RS01455 at 328.400 kb on - strand, within EX31_RS01455 at 328.400 kb on - strand, within EX31_RS01455 at 328.400 kb on - strand, within EX31_RS01455 at 328.408 kb on + strand, within EX31_RS01455 at 328.441 kb on + strand at 328.442 kb on - strand at 328.442 kb on - strand at 328.442 kb on - strand at 328.538 kb on - strand at 328.538 kb on - strand at 328.538 kb on - strand at 328.563 kb on - strand at 328.609 kb on + strand at 328.610 kb on - strand at 328.886 kb on - strand, within EX31_RS01460 at 328.930 kb on - strand, within EX31_RS01460 at 328.930 kb on - strand, within EX31_RS01460 at 328.930 kb on - strand, within EX31_RS01460 at 328.933 kb on - strand, within EX31_RS01460 at 328.944 kb on + strand, within EX31_RS01460 at 328.944 kb on + strand, within EX31_RS01460 at 328.944 kb on + strand, within EX31_RS01460 at 328.945 kb on - strand, within EX31_RS01460 at 328.945 kb on - strand, within EX31_RS01460 at 328.945 kb on - strand, within EX31_RS01460 at 328.945 kb on - strand, within EX31_RS01460 at 328.945 kb on - strand, within EX31_RS01460 at 329.048 kb on - strand, within EX31_RS01460 at 329.048 kb on - strand, within EX31_RS01460 at 329.048 kb on - strand, within EX31_RS01460 at 329.048 kb on - strand, within EX31_RS01460 at 329.048 kb on - strand, within EX31_RS01460 at 329.144 kb on + strand, within EX31_RS01460 at 329.224 kb on + strand, within EX31_RS01460 at 329.265 kb on - strand, within EX31_RS01460 at 329.322 kb on + strand, within EX31_RS01460 at 329.322 kb on + strand, within EX31_RS01460 at 329.343 kb on + strand, within EX31_RS01460 at 329.344 kb on - strand, within EX31_RS01460 at 329.344 kb on - strand, within EX31_RS01460 at 329.355 kb on + strand, within EX31_RS01460 at 329.356 kb on - strand, within EX31_RS01460 at 329.356 kb on - strand, within EX31_RS01460 at 329.370 kb on + strand, within EX31_RS01460 at 329.370 kb on + strand, within EX31_RS01460 at 329.370 kb on + strand, within EX31_RS01460 at 329.370 kb on + strand, within EX31_RS01460 at 329.371 kb on - strand, within EX31_RS01460 at 329.371 kb on - strand, within EX31_RS01460 at 329.371 kb on - strand, within EX31_RS01460 at 329.371 kb on - strand, within EX31_RS01460 at 329.371 kb on - strand, within EX31_RS01460 at 329.371 kb on - strand, within EX31_RS01460 at 329.374 kb on - strand, within EX31_RS01460 at 329.550 kb on + strand, within EX31_RS01460 at 329.550 kb on + strand, within EX31_RS01460 at 329.646 kb on - strand, within EX31_RS01460 at 329.681 kb on - strand, within EX31_RS01460 at 329.681 kb on - strand, within EX31_RS01460 at 329.681 kb on - strand, within EX31_RS01460 at 329.702 kb on - strand, within EX31_RS01460 at 329.762 kb on - strand, within EX31_RS01460 at 329.762 kb on - strand, within EX31_RS01460 at 329.839 kb on + strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 329.840 kb on - strand, within EX31_RS01460 at 330.152 kb on - strand at 330.170 kb on - strand at 330.204 kb on + strand at 330.205 kb on - strand at 330.205 kb on - strand at 330.205 kb on - strand at 330.223 kb on - strand at 330.223 kb on - strand at 330.264 kb on - strand at 330.276 kb on + strand at 330.277 kb on - strand at 330.293 kb on + strand at 330.298 kb on + strand at 330.298 kb on + strand at 330.298 kb on + strand at 330.298 kb on + strand at 330.298 kb on + strand at 330.298 kb on + strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.299 kb on - strand at 330.300 kb on + strand at 330.301 kb on - strand at 330.306 kb on + strand at 330.307 kb on - strand at 330.309 kb on - strand at 330.309 kb on - strand at 330.309 kb on - strand at 330.309 kb on - strand at 330.309 kb on - strand at 330.309 kb on - strand at 330.309 kb on - strand at 330.309 kb on - strand at 330.319 kb on + strand at 330.320 kb on - strand at 330.320 kb on - strand at 330.476 kb on - strand at 330.601 kb on - strand at 330.601 kb on - strand at 330.675 kb on + strand, within EX31_RS01465 at 330.676 kb on - strand, within EX31_RS01465 at 330.679 kb on - strand, within EX31_RS01465 at 330.679 kb on - strand, within EX31_RS01465 at 330.679 kb on - strand, within EX31_RS01465 at 330.771 kb on + strand, within EX31_RS01465 at 330.771 kb on + strand, within EX31_RS01465 at 330.778 kb on - strand, within EX31_RS01465 at 330.780 kb on + strand, within EX31_RS01465 at 330.781 kb on - strand, within EX31_RS01465 at 330.798 kb on - strand, within EX31_RS01465 at 330.811 kb on + strand, within EX31_RS01465 at 330.844 kb on - strand, within EX31_RS01465 at 330.844 kb on - strand, within EX31_RS01465
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 327,746 - EX31_RS01455 0.44 -0.7 327,746 - EX31_RS01455 0.44 +2.3 327,746 - EX31_RS01455 0.44 +1.3 327,778 + EX31_RS01455 0.46 +1.1 327,793 + EX31_RS01455 0.47 -0.6 327,793 + EX31_RS01455 0.47 +2.5 327,794 - EX31_RS01455 0.47 -0.5 327,821 + EX31_RS01455 0.49 +1.7 327,822 - EX31_RS01455 0.49 -0.3 327,923 - EX31_RS01455 0.56 -0.2 327,985 + EX31_RS01455 0.60 +2.3 328,259 + EX31_RS01455 0.79 -0.7 328,260 - EX31_RS01455 0.79 +0.0 328,295 + EX31_RS01455 0.81 +0.4 328,296 - EX31_RS01455 0.81 +0.2 328,326 + EX31_RS01455 0.83 +0.9 328,327 - EX31_RS01455 0.83 -1.3 328,400 - EX31_RS01455 0.88 -0.0 328,400 - EX31_RS01455 0.88 -1.2 328,400 - EX31_RS01455 0.88 -1.5 328,400 - EX31_RS01455 0.88 +0.2 328,400 - EX31_RS01455 0.88 -0.4 328,408 + EX31_RS01455 0.89 -0.3 328,441 + -0.2 328,442 - -1.4 328,442 - +0.6 328,442 - -2.0 328,538 - -0.3 328,538 - +1.0 328,538 - +0.1 328,563 - +1.0 328,609 + -0.6 328,610 - -1.3 328,886 - EX31_RS01460 0.13 +0.6 328,930 - EX31_RS01460 0.16 +0.2 328,930 - EX31_RS01460 0.16 -0.9 328,930 - EX31_RS01460 0.16 +0.2 328,933 - EX31_RS01460 0.16 -0.1 328,944 + EX31_RS01460 0.16 +1.1 328,944 + EX31_RS01460 0.16 +0.1 328,944 + EX31_RS01460 0.16 +0.3 328,945 - EX31_RS01460 0.17 +1.1 328,945 - EX31_RS01460 0.17 +1.4 328,945 - EX31_RS01460 0.17 +1.6 328,945 - EX31_RS01460 0.17 +0.5 328,945 - EX31_RS01460 0.17 +1.3 329,048 - EX31_RS01460 0.23 -0.3 329,048 - EX31_RS01460 0.23 +0.1 329,048 - EX31_RS01460 0.23 -0.3 329,048 - EX31_RS01460 0.23 -0.2 329,048 - EX31_RS01460 0.23 +1.7 329,144 + EX31_RS01460 0.30 +0.9 329,224 + EX31_RS01460 0.35 +0.2 329,265 - EX31_RS01460 0.38 +3.3 329,322 + EX31_RS01460 0.42 +0.4 329,322 + EX31_RS01460 0.42 -0.6 329,343 + EX31_RS01460 0.43 -0.0 329,344 - EX31_RS01460 0.43 -0.0 329,344 - EX31_RS01460 0.43 +0.3 329,355 + EX31_RS01460 0.44 +0.3 329,356 - EX31_RS01460 0.44 -1.5 329,356 - EX31_RS01460 0.44 +0.3 329,370 + EX31_RS01460 0.45 +0.7 329,370 + EX31_RS01460 0.45 -1.0 329,370 + EX31_RS01460 0.45 +1.3 329,370 + EX31_RS01460 0.45 -1.3 329,371 - EX31_RS01460 0.45 -1.3 329,371 - EX31_RS01460 0.45 +0.4 329,371 - EX31_RS01460 0.45 +0.2 329,371 - EX31_RS01460 0.45 +0.3 329,371 - EX31_RS01460 0.45 -0.4 329,371 - EX31_RS01460 0.45 -0.2 329,374 - EX31_RS01460 0.45 -1.0 329,550 + EX31_RS01460 0.57 +2.6 329,550 + EX31_RS01460 0.57 +0.2 329,646 - EX31_RS01460 0.63 +0.0 329,681 - EX31_RS01460 0.65 +2.7 329,681 - EX31_RS01460 0.65 +0.1 329,681 - EX31_RS01460 0.65 +0.5 329,702 - EX31_RS01460 0.67 +0.3 329,762 - EX31_RS01460 0.71 +0.3 329,762 - EX31_RS01460 0.71 -0.9 329,839 + EX31_RS01460 0.76 +1.3 329,840 - EX31_RS01460 0.76 +0.2 329,840 - EX31_RS01460 0.76 +1.0 329,840 - EX31_RS01460 0.76 +1.0 329,840 - EX31_RS01460 0.76 -0.3 329,840 - EX31_RS01460 0.76 -3.0 329,840 - EX31_RS01460 0.76 +1.2 329,840 - EX31_RS01460 0.76 +1.0 329,840 - EX31_RS01460 0.76 +0.4 329,840 - EX31_RS01460 0.76 +0.0 330,152 - +0.1 330,170 - -0.5 330,204 + +1.2 330,205 - -0.0 330,205 - +1.1 330,205 - -0.2 330,223 - -0.7 330,223 - -1.1 330,264 - +1.6 330,276 + +0.7 330,277 - +1.7 330,293 + +1.0 330,298 + +1.0 330,298 + -0.0 330,298 + -1.4 330,298 + +0.2 330,298 + +2.9 330,298 + +1.0 330,299 - +0.9 330,299 - -0.6 330,299 - -1.6 330,299 - -1.5 330,299 - -0.4 330,299 - -0.8 330,299 - +1.3 330,299 - -0.8 330,299 - +1.0 330,299 - +0.4 330,299 - -1.6 330,299 - -0.9 330,299 - -1.9 330,299 - +1.0 330,299 - -0.1 330,299 - +1.5 330,299 - -0.1 330,299 - -0.7 330,299 - +0.4 330,299 - -2.0 330,299 - +0.1 330,300 + -0.9 330,301 - +1.9 330,306 + +0.6 330,307 - +0.0 330,309 - -0.3 330,309 - -0.8 330,309 - -0.7 330,309 - -0.2 330,309 - +0.3 330,309 - +0.4 330,309 - -1.4 330,309 - -1.3 330,319 + -0.0 330,320 - -0.6 330,320 - +0.5 330,476 - +2.3 330,601 - +0.3 330,601 - +1.3 330,675 + EX31_RS01465 0.15 -1.2 330,676 - EX31_RS01465 0.16 +0.3 330,679 - EX31_RS01465 0.16 +0.4 330,679 - EX31_RS01465 0.16 -0.3 330,679 - EX31_RS01465 0.16 +1.3 330,771 + EX31_RS01465 0.24 -0.0 330,771 + EX31_RS01465 0.24 -0.0 330,778 - EX31_RS01465 0.24 -1.0 330,780 + EX31_RS01465 0.24 -0.0 330,781 - EX31_RS01465 0.24 +1.3 330,798 - EX31_RS01465 0.26 -0.2 330,811 + EX31_RS01465 0.27 +1.0 330,844 - EX31_RS01465 0.30 +0.2 330,844 - EX31_RS01465 0.30 -0.3
Or see this region's nucleotide sequence