Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01280 and EX31_RS01285 are separated by 3 nucleotides EX31_RS01285 and EX31_RS01290 are separated by 107 nucleotides EX31_RS01290 and EX31_RS01295 overlap by 10 nucleotides
EX31_RS01280: EX31_RS01280 - SgcJ/EcaC family oxidoreductase, at 284,496 to 284,894
_RS01280
EX31_RS01285: EX31_RS01285 - sulfite exporter TauE/SafE family protein, at 284,898 to 285,623
_RS01285
EX31_RS01290: EX31_RS01290 - LysR family transcriptional regulator, at 285,731 to 286,621
_RS01290
EX31_RS01295: EX31_RS01295 - alpha,alpha-trehalase TreF, at 286,612 to 288,246
_RS01295
Position (kb) 285
286
287 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 284.888 kb on + strand at 284.899 kb on + strand at 284.946 kb on + strand at 284.991 kb on - strand, within EX31_RS01285 at 284.991 kb on - strand, within EX31_RS01285 at 284.991 kb on - strand, within EX31_RS01285 at 284.991 kb on - strand, within EX31_RS01285 at 285.112 kb on + strand, within EX31_RS01285 at 285.113 kb on - strand, within EX31_RS01285 at 285.272 kb on + strand, within EX31_RS01285 at 285.338 kb on + strand, within EX31_RS01285 at 285.339 kb on - strand, within EX31_RS01285 at 285.339 kb on - strand, within EX31_RS01285 at 285.339 kb on - strand, within EX31_RS01285 at 285.371 kb on + strand, within EX31_RS01285 at 285.412 kb on + strand, within EX31_RS01285 at 285.480 kb on - strand, within EX31_RS01285 at 285.526 kb on + strand, within EX31_RS01285 at 285.526 kb on + strand, within EX31_RS01285 at 285.527 kb on - strand, within EX31_RS01285 at 285.595 kb on - strand at 285.619 kb on + strand at 285.619 kb on + strand at 285.619 kb on + strand at 285.619 kb on + strand at 285.619 kb on + strand at 285.619 kb on + strand at 285.619 kb on + strand at 285.619 kb on + strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.620 kb on - strand at 285.665 kb on + strand at 285.707 kb on - strand at 285.707 kb on - strand at 285.707 kb on - strand at 285.816 kb on + strand at 285.816 kb on + strand at 285.816 kb on + strand at 285.817 kb on - strand at 285.817 kb on - strand at 285.883 kb on - strand, within EX31_RS01290 at 286.006 kb on + strand, within EX31_RS01290 at 286.132 kb on - strand, within EX31_RS01290 at 286.188 kb on - strand, within EX31_RS01290 at 286.382 kb on + strand, within EX31_RS01290 at 286.450 kb on + strand, within EX31_RS01290 at 286.505 kb on - strand, within EX31_RS01290 at 286.631 kb on + strand at 286.632 kb on - strand at 286.632 kb on - strand at 286.632 kb on - strand at 286.633 kb on + strand at 286.634 kb on - strand at 286.634 kb on - strand at 286.736 kb on + strand at 286.770 kb on - strand at 286.795 kb on - strand, within EX31_RS01295 at 286.908 kb on - strand, within EX31_RS01295 at 287.000 kb on + strand, within EX31_RS01295 at 287.000 kb on + strand, within EX31_RS01295 at 287.001 kb on - strand, within EX31_RS01295 at 287.001 kb on - strand, within EX31_RS01295 at 287.001 kb on - strand, within EX31_RS01295 at 287.001 kb on - strand, within EX31_RS01295 at 287.094 kb on - strand, within EX31_RS01295 at 287.094 kb on - strand, within EX31_RS01295 at 287.187 kb on + strand, within EX31_RS01295 at 287.615 kb on + strand, within EX31_RS01295 at 287.618 kb on + strand, within EX31_RS01295 at 287.619 kb on - strand, within EX31_RS01295 at 287.619 kb on - strand, within EX31_RS01295 at 287.619 kb on - strand, within EX31_RS01295 at 287.619 kb on - strand, within EX31_RS01295 at 287.619 kb on - strand, within EX31_RS01295
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 284,888 + -1.9 284,899 + -0.3 284,946 + +1.3 284,991 - EX31_RS01285 0.13 +0.7 284,991 - EX31_RS01285 0.13 -0.3 284,991 - EX31_RS01285 0.13 +0.2 284,991 - EX31_RS01285 0.13 +1.3 285,112 + EX31_RS01285 0.29 +0.7 285,113 - EX31_RS01285 0.30 -1.5 285,272 + EX31_RS01285 0.52 -0.2 285,338 + EX31_RS01285 0.61 +0.1 285,339 - EX31_RS01285 0.61 -0.5 285,339 - EX31_RS01285 0.61 -2.2 285,339 - EX31_RS01285 0.61 -0.9 285,371 + EX31_RS01285 0.65 +1.7 285,412 + EX31_RS01285 0.71 -1.3 285,480 - EX31_RS01285 0.80 +0.3 285,526 + EX31_RS01285 0.87 -0.0 285,526 + EX31_RS01285 0.87 +0.8 285,527 - EX31_RS01285 0.87 -0.7 285,595 - +1.2 285,619 + +0.9 285,619 + +0.1 285,619 + -0.9 285,619 + -0.1 285,619 + +0.7 285,619 + +0.1 285,619 + -0.5 285,619 + +0.7 285,620 - +0.8 285,620 - +0.9 285,620 - -0.6 285,620 - +1.3 285,620 - -0.6 285,620 - +0.9 285,620 - -0.4 285,620 - -1.2 285,620 - +2.9 285,620 - +0.0 285,665 + +0.3 285,707 - -0.5 285,707 - -1.0 285,707 - -0.5 285,816 + +1.4 285,816 + +0.4 285,816 + -0.0 285,817 - +1.9 285,817 - -1.0 285,883 - EX31_RS01290 0.17 -0.3 286,006 + EX31_RS01290 0.31 +2.7 286,132 - EX31_RS01290 0.45 -1.7 286,188 - EX31_RS01290 0.51 +1.2 286,382 + EX31_RS01290 0.73 +0.6 286,450 + EX31_RS01290 0.81 +0.5 286,505 - EX31_RS01290 0.87 +0.5 286,631 + -0.4 286,632 - +1.4 286,632 - +0.9 286,632 - +0.7 286,633 + -0.2 286,634 - -0.5 286,634 - +1.6 286,736 + -1.6 286,770 - -0.9 286,795 - EX31_RS01295 0.11 -0.7 286,908 - EX31_RS01295 0.18 -0.5 287,000 + EX31_RS01295 0.24 +0.4 287,000 + EX31_RS01295 0.24 +0.1 287,001 - EX31_RS01295 0.24 -2.0 287,001 - EX31_RS01295 0.24 +0.9 287,001 - EX31_RS01295 0.24 +2.3 287,001 - EX31_RS01295 0.24 +0.7 287,094 - EX31_RS01295 0.29 +0.0 287,094 - EX31_RS01295 0.29 +0.6 287,187 + EX31_RS01295 0.35 -0.0 287,615 + EX31_RS01295 0.61 -0.5 287,618 + EX31_RS01295 0.62 +1.9 287,619 - EX31_RS01295 0.62 +0.0 287,619 - EX31_RS01295 0.62 +1.1 287,619 - EX31_RS01295 0.62 -0.6 287,619 - EX31_RS01295 0.62 -0.0 287,619 - EX31_RS01295 0.62 -0.2
Or see this region's nucleotide sequence