Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01115 and EX31_RS01120 are separated by 351 nucleotides EX31_RS01120 and EX31_RS01125 are separated by 138 nucleotides
EX31_RS01115: EX31_RS01115 - ABC-F family ATP-binding cassette domain-containing protein, at 242,241 to 243,974
_RS01115
EX31_RS01120: EX31_RS01120 - MFS transporter, at 244,326 to 245,564
_RS01120
EX31_RS01125: EX31_RS01125 - alpha/beta hydrolase, at 245,703 to 246,626
_RS01125
Position (kb) 244
245
246 Strain fitness (log2 ratio) -2
-1
0
1
2 at 243.492 kb on + strand, within EX31_RS01115 at 243.493 kb on - strand, within EX31_RS01115 at 243.537 kb on + strand, within EX31_RS01115 at 243.725 kb on + strand, within EX31_RS01115 at 243.725 kb on + strand, within EX31_RS01115 at 243.726 kb on - strand, within EX31_RS01115 at 243.726 kb on - strand, within EX31_RS01115 at 243.726 kb on - strand, within EX31_RS01115 at 243.945 kb on + strand at 243.945 kb on + strand at 243.964 kb on - strand at 243.964 kb on - strand at 243.964 kb on - strand at 243.983 kb on - strand at 243.983 kb on - strand at 244.038 kb on + strand at 244.069 kb on + strand at 244.070 kb on - strand at 244.183 kb on + strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.187 kb on + strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.214 kb on - strand at 244.215 kb on + strand at 244.554 kb on - strand, within EX31_RS01120 at 244.563 kb on + strand, within EX31_RS01120 at 244.595 kb on + strand, within EX31_RS01120 at 244.782 kb on + strand, within EX31_RS01120 at 244.783 kb on - strand, within EX31_RS01120 at 244.783 kb on - strand, within EX31_RS01120 at 244.792 kb on - strand, within EX31_RS01120 at 244.860 kb on + strand, within EX31_RS01120 at 244.986 kb on + strand, within EX31_RS01120 at 245.006 kb on + strand, within EX31_RS01120 at 245.006 kb on + strand, within EX31_RS01120 at 245.010 kb on + strand, within EX31_RS01120 at 245.010 kb on + strand, within EX31_RS01120 at 245.017 kb on + strand, within EX31_RS01120 at 245.017 kb on + strand, within EX31_RS01120 at 245.018 kb on - strand, within EX31_RS01120 at 245.179 kb on - strand, within EX31_RS01120 at 245.437 kb on + strand, within EX31_RS01120 at 245.529 kb on + strand at 245.668 kb on + strand at 245.668 kb on + strand at 245.669 kb on - strand at 245.669 kb on - strand at 245.669 kb on - strand at 245.669 kb on - strand at 245.669 kb on - strand at 245.669 kb on - strand at 245.669 kb on - strand at 245.720 kb on + strand at 245.721 kb on - strand at 245.721 kb on - strand at 245.721 kb on - strand at 245.721 kb on - strand at 245.721 kb on - strand at 245.880 kb on + strand, within EX31_RS01125 at 245.898 kb on - strand, within EX31_RS01125 at 245.938 kb on + strand, within EX31_RS01125 at 246.213 kb on + strand, within EX31_RS01125 at 246.214 kb on - strand, within EX31_RS01125 at 246.226 kb on + strand, within EX31_RS01125 at 246.226 kb on + strand, within EX31_RS01125 at 246.226 kb on + strand, within EX31_RS01125 at 246.226 kb on + strand, within EX31_RS01125 at 246.445 kb on + strand, within EX31_RS01125 at 246.511 kb on + strand, within EX31_RS01125 at 246.512 kb on - strand, within EX31_RS01125 at 246.512 kb on - strand, within EX31_RS01125 at 246.512 kb on - strand, within EX31_RS01125
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 243,492 + EX31_RS01115 0.72 -1.0 243,493 - EX31_RS01115 0.72 -1.6 243,537 + EX31_RS01115 0.75 +0.5 243,725 + EX31_RS01115 0.86 -0.3 243,725 + EX31_RS01115 0.86 -1.0 243,726 - EX31_RS01115 0.86 -0.1 243,726 - EX31_RS01115 0.86 -0.7 243,726 - EX31_RS01115 0.86 -1.3 243,945 + -0.6 243,945 + -0.0 243,964 - -0.9 243,964 - -0.0 243,964 - +0.1 243,983 - +0.5 243,983 - +0.7 244,038 + +0.7 244,069 + +0.2 244,070 - -2.2 244,183 + -0.8 244,184 - +1.5 244,184 - +0.8 244,184 - +0.5 244,184 - +2.3 244,184 - +1.0 244,185 + +1.7 244,185 + -0.8 244,185 + +0.4 244,185 + -0.0 244,185 + -0.3 244,186 - -1.0 244,186 - +0.4 244,186 - -0.6 244,186 - -1.8 244,186 - -1.5 244,186 - +0.2 244,186 - +0.1 244,186 - -0.2 244,187 + +0.7 244,188 - -1.0 244,188 - -1.4 244,188 - +0.3 244,188 - +0.7 244,188 - -0.9 244,188 - +0.0 244,188 - +1.2 244,214 - +1.3 244,215 + -0.8 244,554 - EX31_RS01120 0.18 +0.6 244,563 + EX31_RS01120 0.19 -0.0 244,595 + EX31_RS01120 0.22 +0.3 244,782 + EX31_RS01120 0.37 +0.5 244,783 - EX31_RS01120 0.37 -0.7 244,783 - EX31_RS01120 0.37 +1.8 244,792 - EX31_RS01120 0.38 -0.4 244,860 + EX31_RS01120 0.43 +0.1 244,986 + EX31_RS01120 0.53 +0.1 245,006 + EX31_RS01120 0.55 -0.7 245,006 + EX31_RS01120 0.55 +0.2 245,010 + EX31_RS01120 0.55 +1.3 245,010 + EX31_RS01120 0.55 -0.3 245,017 + EX31_RS01120 0.56 -0.0 245,017 + EX31_RS01120 0.56 -1.7 245,018 - EX31_RS01120 0.56 -0.2 245,179 - EX31_RS01120 0.69 +0.1 245,437 + EX31_RS01120 0.90 -0.2 245,529 + +1.2 245,668 + +0.7 245,668 + +0.8 245,669 - -0.0 245,669 - -0.0 245,669 - -0.0 245,669 - -1.0 245,669 - +1.3 245,669 - -0.1 245,669 - +0.7 245,720 + -0.1 245,721 - +0.0 245,721 - +0.7 245,721 - -0.9 245,721 - -0.8 245,721 - +0.5 245,880 + EX31_RS01125 0.19 +2.1 245,898 - EX31_RS01125 0.21 -0.4 245,938 + EX31_RS01125 0.25 -0.1 246,213 + EX31_RS01125 0.55 +0.5 246,214 - EX31_RS01125 0.55 -0.5 246,226 + EX31_RS01125 0.57 +0.6 246,226 + EX31_RS01125 0.57 -1.0 246,226 + EX31_RS01125 0.57 -0.2 246,226 + EX31_RS01125 0.57 -0.5 246,445 + EX31_RS01125 0.80 -0.5 246,511 + EX31_RS01125 0.87 +0.1 246,512 - EX31_RS01125 0.88 +0.6 246,512 - EX31_RS01125 0.88 -0.2 246,512 - EX31_RS01125 0.88 -1.2
Or see this region's nucleotide sequence