Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01095 and EX31_RS01100 are separated by 139 nucleotides EX31_RS01100 and EX31_RS01105 are separated by 166 nucleotides EX31_RS01105 and EX31_RS01110 are separated by 943 nucleotides
EX31_RS01095: EX31_RS01095 - metal ABC transporter permease, at 237,908 to 238,762
_RS01095
EX31_RS01100: EX31_RS01100 - hypothetical protein, at 238,902 to 239,081
_RS01100
EX31_RS01105: EX31_RS01105 - tryptophan permease, at 239,248 to 240,492
_RS01105
EX31_RS01110: EX31_RS01110 - hypothetical protein, at 241,436 to 242,170
_RS01110
Position (kb) 239
240
241 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 238.434 kb on + strand, within EX31_RS01095 at 238.448 kb on + strand, within EX31_RS01095 at 238.449 kb on - strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.462 kb on + strand, within EX31_RS01095 at 238.463 kb on - strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.640 kb on + strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.641 kb on - strand, within EX31_RS01095 at 238.642 kb on + strand, within EX31_RS01095 at 238.642 kb on + strand, within EX31_RS01095 at 238.756 kb on + strand at 238.760 kb on + strand at 238.760 kb on + strand at 238.760 kb on + strand at 238.841 kb on - strand at 238.884 kb on + strand at 238.884 kb on + strand at 238.885 kb on - strand at 238.901 kb on + strand at 238.901 kb on + strand at 238.901 kb on + strand at 238.901 kb on + strand at 239.073 kb on - strand at 239.079 kb on + strand at 239.117 kb on + strand at 239.189 kb on - strand at 239.189 kb on - strand at 239.191 kb on - strand at 239.191 kb on - strand at 239.191 kb on - strand at 239.196 kb on - strand at 239.258 kb on - strand at 239.273 kb on - strand at 239.337 kb on + strand at 239.358 kb on - strand at 239.358 kb on - strand at 239.461 kb on - strand, within EX31_RS01105 at 239.475 kb on - strand, within EX31_RS01105 at 239.571 kb on - strand, within EX31_RS01105 at 239.698 kb on - strand, within EX31_RS01105 at 239.698 kb on - strand, within EX31_RS01105 at 239.698 kb on - strand, within EX31_RS01105 at 239.774 kb on + strand, within EX31_RS01105 at 239.774 kb on + strand, within EX31_RS01105 at 239.775 kb on - strand, within EX31_RS01105 at 239.775 kb on - strand, within EX31_RS01105 at 239.775 kb on - strand, within EX31_RS01105 at 239.775 kb on - strand, within EX31_RS01105 at 239.775 kb on - strand, within EX31_RS01105 at 239.791 kb on - strand, within EX31_RS01105 at 239.844 kb on - strand, within EX31_RS01105 at 239.861 kb on - strand, within EX31_RS01105 at 239.915 kb on + strand, within EX31_RS01105 at 239.915 kb on + strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.916 kb on - strand, within EX31_RS01105 at 239.924 kb on + strand, within EX31_RS01105 at 239.924 kb on + strand, within EX31_RS01105 at 239.924 kb on + strand, within EX31_RS01105 at 239.924 kb on + strand, within EX31_RS01105 at 239.924 kb on + strand, within EX31_RS01105 at 239.925 kb on - strand, within EX31_RS01105 at 239.980 kb on + strand, within EX31_RS01105 at 239.981 kb on - strand, within EX31_RS01105 at 239.981 kb on - strand, within EX31_RS01105 at 239.981 kb on - strand, within EX31_RS01105 at 240.145 kb on - strand, within EX31_RS01105 at 240.145 kb on - strand, within EX31_RS01105 at 240.145 kb on - strand, within EX31_RS01105 at 240.145 kb on - strand, within EX31_RS01105 at 240.174 kb on + strand, within EX31_RS01105 at 240.175 kb on - strand, within EX31_RS01105 at 240.175 kb on - strand, within EX31_RS01105 at 240.175 kb on - strand, within EX31_RS01105 at 240.176 kb on + strand, within EX31_RS01105 at 240.176 kb on + strand, within EX31_RS01105 at 240.176 kb on + strand, within EX31_RS01105 at 240.176 kb on + strand, within EX31_RS01105 at 240.182 kb on + strand, within EX31_RS01105 at 240.182 kb on + strand, within EX31_RS01105 at 240.182 kb on + strand, within EX31_RS01105 at 240.182 kb on + strand, within EX31_RS01105 at 240.183 kb on - strand, within EX31_RS01105 at 240.183 kb on - strand, within EX31_RS01105 at 240.183 kb on - strand, within EX31_RS01105 at 240.183 kb on - strand, within EX31_RS01105 at 240.183 kb on - strand, within EX31_RS01105 at 240.183 kb on - strand, within EX31_RS01105 at 240.183 kb on - strand, within EX31_RS01105 at 240.194 kb on + strand, within EX31_RS01105 at 240.194 kb on + strand, within EX31_RS01105 at 240.195 kb on - strand, within EX31_RS01105 at 240.195 kb on - strand, within EX31_RS01105 at 240.195 kb on - strand, within EX31_RS01105 at 240.195 kb on - strand, within EX31_RS01105 at 240.195 kb on - strand, within EX31_RS01105 at 240.268 kb on - strand, within EX31_RS01105 at 240.281 kb on - strand, within EX31_RS01105 at 240.287 kb on + strand, within EX31_RS01105 at 240.291 kb on + strand, within EX31_RS01105 at 240.291 kb on + strand, within EX31_RS01105 at 240.292 kb on - strand, within EX31_RS01105 at 240.292 kb on - strand, within EX31_RS01105 at 240.292 kb on - strand, within EX31_RS01105 at 240.292 kb on - strand, within EX31_RS01105 at 240.331 kb on - strand, within EX31_RS01105 at 240.331 kb on - strand, within EX31_RS01105 at 240.423 kb on + strand at 240.423 kb on + strand at 240.423 kb on + strand at 240.424 kb on - strand at 240.424 kb on - strand at 240.455 kb on - strand at 240.492 kb on + strand at 240.530 kb on - strand at 240.584 kb on - strand at 240.614 kb on + strand at 240.614 kb on + strand at 240.614 kb on + strand at 240.614 kb on + strand at 240.615 kb on - strand at 240.615 kb on - strand at 240.615 kb on - strand at 240.669 kb on + strand at 240.669 kb on + strand at 240.744 kb on + strand at 240.864 kb on + strand at 240.865 kb on - strand at 240.901 kb on + strand at 240.940 kb on + strand at 240.940 kb on + strand at 240.940 kb on + strand at 240.967 kb on + strand at 240.972 kb on + strand at 241.004 kb on + strand at 241.093 kb on + strand at 241.125 kb on + strand at 241.177 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.179 kb on + strand at 241.197 kb on + strand at 241.226 kb on + strand at 241.226 kb on + strand at 241.266 kb on + strand at 241.312 kb on + strand at 241.312 kb on + strand at 241.333 kb on + strand at 241.333 kb on + strand at 241.359 kb on + strand at 241.374 kb on + strand at 241.382 kb on + strand at 241.438 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 238,434 + EX31_RS01095 0.62 -0.7 238,448 + EX31_RS01095 0.63 +0.3 238,449 - EX31_RS01095 0.63 -1.4 238,462 + EX31_RS01095 0.65 +0.5 238,462 + EX31_RS01095 0.65 +0.3 238,462 + EX31_RS01095 0.65 +0.6 238,462 + EX31_RS01095 0.65 -0.0 238,462 + EX31_RS01095 0.65 -0.1 238,462 + EX31_RS01095 0.65 -0.9 238,463 - EX31_RS01095 0.65 +0.4 238,640 + EX31_RS01095 0.86 +0.6 238,640 + EX31_RS01095 0.86 +0.3 238,640 + EX31_RS01095 0.86 +0.8 238,640 + EX31_RS01095 0.86 +0.9 238,641 - EX31_RS01095 0.86 +1.5 238,641 - EX31_RS01095 0.86 +0.1 238,641 - EX31_RS01095 0.86 +0.3 238,641 - EX31_RS01095 0.86 -0.2 238,642 + EX31_RS01095 0.86 +0.7 238,642 + EX31_RS01095 0.86 +0.6 238,756 + +1.4 238,760 + +0.7 238,760 + -1.2 238,760 + +0.3 238,841 - +0.1 238,884 + +2.5 238,884 + +0.7 238,885 - +0.4 238,901 + +0.3 238,901 + +0.3 238,901 + +2.3 238,901 + -0.2 239,073 - -1.7 239,079 + -0.3 239,117 + +1.0 239,189 - +1.7 239,189 - -0.2 239,191 - -1.0 239,191 - +2.3 239,191 - -1.2 239,196 - -1.0 239,258 - -0.5 239,273 - +1.7 239,337 + -0.7 239,358 - +0.3 239,358 - +1.5 239,461 - EX31_RS01105 0.17 -1.4 239,475 - EX31_RS01105 0.18 +0.2 239,571 - EX31_RS01105 0.26 -0.4 239,698 - EX31_RS01105 0.36 +1.9 239,698 - EX31_RS01105 0.36 +1.5 239,698 - EX31_RS01105 0.36 +0.8 239,774 + EX31_RS01105 0.42 +1.3 239,774 + EX31_RS01105 0.42 +1.5 239,775 - EX31_RS01105 0.42 -0.2 239,775 - EX31_RS01105 0.42 -0.1 239,775 - EX31_RS01105 0.42 +1.7 239,775 - EX31_RS01105 0.42 -1.6 239,775 - EX31_RS01105 0.42 -1.1 239,791 - EX31_RS01105 0.44 +1.1 239,844 - EX31_RS01105 0.48 -0.9 239,861 - EX31_RS01105 0.49 -0.5 239,915 + EX31_RS01105 0.54 +0.1 239,915 + EX31_RS01105 0.54 -0.7 239,916 - EX31_RS01105 0.54 +0.3 239,916 - EX31_RS01105 0.54 -0.0 239,916 - EX31_RS01105 0.54 -0.1 239,916 - EX31_RS01105 0.54 +0.7 239,916 - EX31_RS01105 0.54 +1.1 239,916 - EX31_RS01105 0.54 -1.6 239,916 - EX31_RS01105 0.54 +0.1 239,916 - EX31_RS01105 0.54 +0.7 239,916 - EX31_RS01105 0.54 -0.0 239,916 - EX31_RS01105 0.54 -0.5 239,916 - EX31_RS01105 0.54 -1.1 239,924 + EX31_RS01105 0.54 +0.1 239,924 + EX31_RS01105 0.54 -0.0 239,924 + EX31_RS01105 0.54 -0.5 239,924 + EX31_RS01105 0.54 +0.3 239,924 + EX31_RS01105 0.54 +1.9 239,925 - EX31_RS01105 0.54 +0.5 239,980 + EX31_RS01105 0.59 -0.3 239,981 - EX31_RS01105 0.59 +0.8 239,981 - EX31_RS01105 0.59 +1.4 239,981 - EX31_RS01105 0.59 +0.6 240,145 - EX31_RS01105 0.72 -0.5 240,145 - EX31_RS01105 0.72 +0.6 240,145 - EX31_RS01105 0.72 -0.4 240,145 - EX31_RS01105 0.72 -2.4 240,174 + EX31_RS01105 0.74 +0.7 240,175 - EX31_RS01105 0.74 -1.2 240,175 - EX31_RS01105 0.74 +0.6 240,175 - EX31_RS01105 0.74 +0.4 240,176 + EX31_RS01105 0.75 +0.4 240,176 + EX31_RS01105 0.75 +1.0 240,176 + EX31_RS01105 0.75 +1.7 240,176 + EX31_RS01105 0.75 -1.5 240,182 + EX31_RS01105 0.75 -1.0 240,182 + EX31_RS01105 0.75 +0.3 240,182 + EX31_RS01105 0.75 +1.1 240,182 + EX31_RS01105 0.75 -2.1 240,183 - EX31_RS01105 0.75 -2.1 240,183 - EX31_RS01105 0.75 +0.5 240,183 - EX31_RS01105 0.75 +0.3 240,183 - EX31_RS01105 0.75 -0.1 240,183 - EX31_RS01105 0.75 -0.5 240,183 - EX31_RS01105 0.75 -0.9 240,183 - EX31_RS01105 0.75 +0.4 240,194 + EX31_RS01105 0.76 +0.3 240,194 + EX31_RS01105 0.76 -0.2 240,195 - EX31_RS01105 0.76 +0.7 240,195 - EX31_RS01105 0.76 -0.1 240,195 - EX31_RS01105 0.76 -1.4 240,195 - EX31_RS01105 0.76 +1.0 240,195 - EX31_RS01105 0.76 -0.0 240,268 - EX31_RS01105 0.82 -1.5 240,281 - EX31_RS01105 0.83 -2.3 240,287 + EX31_RS01105 0.83 +1.1 240,291 + EX31_RS01105 0.84 -0.6 240,291 + EX31_RS01105 0.84 -0.5 240,292 - EX31_RS01105 0.84 -0.9 240,292 - EX31_RS01105 0.84 -1.0 240,292 - EX31_RS01105 0.84 +0.5 240,292 - EX31_RS01105 0.84 +0.3 240,331 - EX31_RS01105 0.87 -0.3 240,331 - EX31_RS01105 0.87 +0.5 240,423 + -0.1 240,423 + +1.8 240,423 + +0.4 240,424 - -0.0 240,424 - -0.5 240,455 - +1.4 240,492 + +0.0 240,530 - +0.7 240,584 - +0.1 240,614 + +2.3 240,614 + +3.9 240,614 + -0.1 240,614 + +1.3 240,615 - +0.1 240,615 - +0.3 240,615 - +0.3 240,669 + +0.2 240,669 + +1.1 240,744 + +0.3 240,864 + -1.8 240,865 - +0.4 240,901 + -0.2 240,940 + -0.0 240,940 + +0.4 240,940 + +0.7 240,967 + +0.3 240,972 + -0.0 241,004 + -0.9 241,093 + -0.2 241,125 + +1.6 241,177 + -0.4 241,179 + -0.5 241,179 + -0.2 241,179 + +0.8 241,179 + -2.3 241,179 + -0.2 241,197 + -2.4 241,226 + -1.5 241,226 + +0.3 241,266 + -0.3 241,312 + -0.2 241,312 + -0.4 241,333 + -1.3 241,333 + -0.8 241,359 + -0.6 241,374 + -0.5 241,382 + +0.6 241,438 - +1.3
Or see this region's nucleotide sequence