Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00660 and EX31_RS00665 overlap by 4 nucleotides EX31_RS00665 and EX31_RS00670 are separated by 11 nucleotides EX31_RS00670 and EX31_RS25040 are separated by 58 nucleotides EX31_RS25040 and EX31_RS00675 are separated by 170 nucleotides
EX31_RS00660: EX31_RS00660 - pyrroloquinoline quinone biosynthesis peptide chaperone PqqD, at 137,826 to 138,113
_RS00660
EX31_RS00665: EX31_RS00665 - pyrroloquinoline-quinone synthase PqqC, at 138,110 to 138,877
_RS00665
EX31_RS00670: EX31_RS00670 - pyrroloquinoline quinone biosynthesis protein PqqB, at 138,889 to 139,800
_RS00670
EX31_RS25040: EX31_RS25040 - pyrroloquinoline quinone precursor peptide PqqA, at 139,859 to 139,930
_RS25040
EX31_RS00675: EX31_RS00675 - membrane dipeptidase, at 140,101 to 141,138
_RS00675
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 138.091 kb on + strand at 138.091 kb on + strand at 138.091 kb on + strand at 138.091 kb on + strand at 138.092 kb on - strand at 138.092 kb on - strand at 138.128 kb on - strand at 138.128 kb on - strand at 138.160 kb on - strand at 138.160 kb on - strand at 138.343 kb on - strand, within EX31_RS00665 at 138.372 kb on + strand, within EX31_RS00665 at 138.373 kb on - strand, within EX31_RS00665 at 138.373 kb on - strand, within EX31_RS00665 at 138.373 kb on - strand, within EX31_RS00665 at 138.373 kb on - strand, within EX31_RS00665 at 138.373 kb on - strand, within EX31_RS00665 at 138.373 kb on - strand, within EX31_RS00665 at 138.479 kb on - strand, within EX31_RS00665 at 138.484 kb on - strand, within EX31_RS00665 at 138.484 kb on - strand, within EX31_RS00665 at 138.484 kb on - strand, within EX31_RS00665 at 138.626 kb on - strand, within EX31_RS00665 at 138.673 kb on + strand, within EX31_RS00665 at 138.674 kb on - strand, within EX31_RS00665 at 138.674 kb on - strand, within EX31_RS00665 at 138.708 kb on + strand, within EX31_RS00665 at 138.711 kb on + strand, within EX31_RS00665 at 138.712 kb on - strand, within EX31_RS00665 at 138.712 kb on - strand, within EX31_RS00665 at 138.712 kb on - strand, within EX31_RS00665 at 138.764 kb on - strand, within EX31_RS00665 at 138.776 kb on - strand, within EX31_RS00665 at 138.776 kb on - strand, within EX31_RS00665 at 138.798 kb on + strand, within EX31_RS00665 at 138.799 kb on - strand, within EX31_RS00665 at 138.891 kb on - strand at 138.984 kb on + strand, within EX31_RS00670 at 138.984 kb on + strand, within EX31_RS00670 at 138.984 kb on + strand, within EX31_RS00670 at 138.985 kb on - strand, within EX31_RS00670 at 138.985 kb on - strand, within EX31_RS00670 at 138.985 kb on - strand, within EX31_RS00670 at 138.985 kb on - strand, within EX31_RS00670 at 138.985 kb on - strand, within EX31_RS00670 at 138.988 kb on - strand, within EX31_RS00670 at 138.988 kb on - strand, within EX31_RS00670 at 138.991 kb on - strand, within EX31_RS00670 at 138.991 kb on - strand, within EX31_RS00670 at 138.991 kb on - strand, within EX31_RS00670 at 138.993 kb on + strand, within EX31_RS00670 at 138.994 kb on - strand, within EX31_RS00670 at 138.994 kb on - strand, within EX31_RS00670 at 139.165 kb on - strand, within EX31_RS00670 at 139.165 kb on - strand, within EX31_RS00670 at 139.215 kb on - strand, within EX31_RS00670 at 139.215 kb on - strand, within EX31_RS00670 at 139.290 kb on - strand, within EX31_RS00670 at 139.787 kb on - strand at 139.787 kb on - strand at 139.787 kb on - strand at 139.787 kb on - strand at 139.791 kb on + strand at 139.841 kb on - strand at 139.860 kb on + strand at 139.861 kb on - strand at 139.875 kb on + strand, within EX31_RS25040 at 139.875 kb on + strand, within EX31_RS25040 at 139.876 kb on - strand, within EX31_RS25040 at 139.889 kb on + strand, within EX31_RS25040 at 139.890 kb on - strand, within EX31_RS25040 at 139.896 kb on - strand, within EX31_RS25040 at 139.922 kb on - strand, within EX31_RS25040 at 139.922 kb on - strand, within EX31_RS25040 at 139.930 kb on + strand at 139.930 kb on + strand at 139.930 kb on + strand at 139.930 kb on + strand at 139.930 kb on + strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.931 kb on - strand at 139.934 kb on + strand at 139.934 kb on + strand at 139.934 kb on + strand at 139.934 kb on + strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.935 kb on - strand at 139.951 kb on + strand at 139.959 kb on + strand at 139.959 kb on + strand at 139.960 kb on - strand at 139.977 kb on - strand at 139.998 kb on + strand at 139.999 kb on - strand at 139.999 kb on - strand at 139.999 kb on - strand at 139.999 kb on - strand at 139.999 kb on - strand at 140.009 kb on - strand at 140.064 kb on - strand at 140.102 kb on + strand at 140.292 kb on + strand, within EX31_RS00675 at 140.347 kb on - strand, within EX31_RS00675 at 140.458 kb on + strand, within EX31_RS00675 at 140.672 kb on - strand, within EX31_RS00675 at 140.763 kb on + strand, within EX31_RS00675
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 138,091 + -0.0 138,091 + +0.7 138,091 + +1.3 138,091 + -0.3 138,092 - +0.4 138,092 - -1.2 138,128 - +0.8 138,128 - +0.5 138,160 - +0.4 138,160 - -1.4 138,343 - EX31_RS00665 0.30 +0.3 138,372 + EX31_RS00665 0.34 -0.4 138,373 - EX31_RS00665 0.34 +0.1 138,373 - EX31_RS00665 0.34 -0.9 138,373 - EX31_RS00665 0.34 +0.5 138,373 - EX31_RS00665 0.34 -0.4 138,373 - EX31_RS00665 0.34 +0.2 138,373 - EX31_RS00665 0.34 +1.7 138,479 - EX31_RS00665 0.48 +1.3 138,484 - EX31_RS00665 0.49 -2.0 138,484 - EX31_RS00665 0.49 -0.8 138,484 - EX31_RS00665 0.49 -0.3 138,626 - EX31_RS00665 0.67 +0.3 138,673 + EX31_RS00665 0.73 +0.7 138,674 - EX31_RS00665 0.73 +0.0 138,674 - EX31_RS00665 0.73 -0.6 138,708 + EX31_RS00665 0.78 +0.2 138,711 + EX31_RS00665 0.78 +0.4 138,712 - EX31_RS00665 0.78 +0.4 138,712 - EX31_RS00665 0.78 +3.3 138,712 - EX31_RS00665 0.78 -0.8 138,764 - EX31_RS00665 0.85 +0.2 138,776 - EX31_RS00665 0.87 -1.4 138,776 - EX31_RS00665 0.87 -0.6 138,798 + EX31_RS00665 0.90 +1.4 138,799 - EX31_RS00665 0.90 -1.2 138,891 - +0.2 138,984 + EX31_RS00670 0.10 +3.3 138,984 + EX31_RS00670 0.10 -0.2 138,984 + EX31_RS00670 0.10 +0.7 138,985 - EX31_RS00670 0.11 -0.4 138,985 - EX31_RS00670 0.11 -0.7 138,985 - EX31_RS00670 0.11 +0.9 138,985 - EX31_RS00670 0.11 +0.4 138,985 - EX31_RS00670 0.11 +0.8 138,988 - EX31_RS00670 0.11 +0.5 138,988 - EX31_RS00670 0.11 -0.4 138,991 - EX31_RS00670 0.11 +0.3 138,991 - EX31_RS00670 0.11 +0.2 138,991 - EX31_RS00670 0.11 +0.1 138,993 + EX31_RS00670 0.11 +0.3 138,994 - EX31_RS00670 0.12 +1.8 138,994 - EX31_RS00670 0.12 +0.2 139,165 - EX31_RS00670 0.30 +0.9 139,165 - EX31_RS00670 0.30 -1.7 139,215 - EX31_RS00670 0.36 +0.6 139,215 - EX31_RS00670 0.36 +2.3 139,290 - EX31_RS00670 0.44 +0.3 139,787 - -0.9 139,787 - +0.6 139,787 - +2.3 139,787 - -0.3 139,791 + -0.0 139,841 - -0.4 139,860 + -0.3 139,861 - +0.1 139,875 + EX31_RS25040 0.22 -0.3 139,875 + EX31_RS25040 0.22 +0.8 139,876 - EX31_RS25040 0.24 -1.5 139,889 + EX31_RS25040 0.42 +0.3 139,890 - EX31_RS25040 0.43 +0.3 139,896 - EX31_RS25040 0.51 -1.0 139,922 - EX31_RS25040 0.88 -0.1 139,922 - EX31_RS25040 0.88 -1.7 139,930 + +0.3 139,930 + +0.6 139,930 + +0.0 139,930 + +0.1 139,930 + +0.4 139,931 - +1.9 139,931 - +0.0 139,931 - +0.6 139,931 - -1.8 139,931 - -0.8 139,931 - +1.4 139,931 - +0.3 139,931 - +0.8 139,934 + +0.4 139,934 + -0.6 139,934 + -1.7 139,934 + -2.3 139,935 - +0.3 139,935 - -0.3 139,935 - +0.7 139,935 - +0.9 139,935 - +0.4 139,935 - -0.2 139,951 + +1.7 139,959 + -0.2 139,959 + +0.4 139,960 - -1.6 139,977 - +0.4 139,998 + +0.3 139,999 - +0.4 139,999 - -0.0 139,999 - +0.7 139,999 - -1.6 139,999 - -1.3 140,009 - -0.6 140,064 - +0.3 140,102 + -0.5 140,292 + EX31_RS00675 0.18 +1.9 140,347 - EX31_RS00675 0.24 -0.2 140,458 + EX31_RS00675 0.34 -0.2 140,672 - EX31_RS00675 0.55 +0.2 140,763 + EX31_RS00675 0.64 +0.1
Or see this region's nucleotide sequence