Experiment: L-Glutamine (N); ShewMM media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00310 and EX31_RS00315 are separated by 111 nucleotides EX31_RS00315 and EX31_RS00320 are separated by 55 nucleotides EX31_RS00320 and EX31_RS00325 are separated by 66 nucleotides
EX31_RS00310: EX31_RS00310 - LysR family transcriptional regulator, at 66,031 to 66,912
_RS00310
EX31_RS00315: EX31_RS00315 - heme-binding protein, at 67,024 to 67,527
_RS00315
EX31_RS00320: EX31_RS00320 - HAD-IA family hydrolase, at 67,583 to 68,272
_RS00320
EX31_RS00325: EX31_RS00325 - hypothetical protein, at 68,339 to 68,983
_RS00325
Position (kb) 67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 66.130 kb on - strand, within EX31_RS00310 at 66.224 kb on - strand, within EX31_RS00310 at 66.224 kb on - strand, within EX31_RS00310 at 66.267 kb on + strand, within EX31_RS00310 at 66.267 kb on + strand, within EX31_RS00310 at 66.268 kb on - strand, within EX31_RS00310 at 66.268 kb on - strand, within EX31_RS00310 at 66.361 kb on + strand, within EX31_RS00310 at 66.361 kb on + strand, within EX31_RS00310 at 66.361 kb on + strand, within EX31_RS00310 at 66.361 kb on + strand, within EX31_RS00310 at 66.362 kb on - strand, within EX31_RS00310 at 66.362 kb on - strand, within EX31_RS00310 at 66.488 kb on - strand, within EX31_RS00310 at 66.530 kb on - strand, within EX31_RS00310 at 66.549 kb on + strand, within EX31_RS00310 at 66.623 kb on + strand, within EX31_RS00310 at 66.623 kb on + strand, within EX31_RS00310 at 66.623 kb on + strand, within EX31_RS00310 at 66.623 kb on + strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.624 kb on - strand, within EX31_RS00310 at 66.628 kb on + strand, within EX31_RS00310 at 66.629 kb on - strand, within EX31_RS00310 at 66.629 kb on - strand, within EX31_RS00310 at 66.629 kb on - strand, within EX31_RS00310 at 66.629 kb on - strand, within EX31_RS00310 at 66.724 kb on + strand, within EX31_RS00310 at 66.725 kb on - strand, within EX31_RS00310 at 66.773 kb on - strand, within EX31_RS00310 at 66.904 kb on + strand at 66.904 kb on + strand at 66.904 kb on + strand at 66.904 kb on + strand at 66.904 kb on + strand at 66.904 kb on + strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.905 kb on - strand at 66.915 kb on + strand at 66.915 kb on + strand at 67.019 kb on + strand at 67.020 kb on - strand at 67.020 kb on - strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.023 kb on + strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.024 kb on - strand at 67.027 kb on + strand at 67.027 kb on + strand at 67.028 kb on - strand at 67.058 kb on + strand at 67.058 kb on + strand at 67.058 kb on + strand at 67.059 kb on - strand at 67.059 kb on - strand at 67.059 kb on - strand at 67.059 kb on - strand at 67.152 kb on - strand, within EX31_RS00315 at 67.242 kb on - strand, within EX31_RS00315 at 67.242 kb on - strand, within EX31_RS00315 at 67.242 kb on - strand, within EX31_RS00315 at 67.242 kb on - strand, within EX31_RS00315 at 67.335 kb on - strand, within EX31_RS00315 at 67.419 kb on + strand, within EX31_RS00315 at 67.747 kb on + strand, within EX31_RS00320 at 67.803 kb on + strand, within EX31_RS00320 at 67.803 kb on + strand, within EX31_RS00320 at 67.803 kb on + strand, within EX31_RS00320 at 67.803 kb on + strand, within EX31_RS00320 at 67.837 kb on + strand, within EX31_RS00320 at 67.837 kb on + strand, within EX31_RS00320 at 67.837 kb on + strand, within EX31_RS00320 at 67.886 kb on + strand, within EX31_RS00320 at 67.896 kb on + strand, within EX31_RS00320 at 67.897 kb on - strand, within EX31_RS00320 at 67.910 kb on - strand, within EX31_RS00320 at 67.954 kb on + strand, within EX31_RS00320 at 67.955 kb on - strand, within EX31_RS00320 at 67.978 kb on + strand, within EX31_RS00320 at 68.006 kb on + strand, within EX31_RS00320 at 68.006 kb on + strand, within EX31_RS00320 at 68.006 kb on + strand, within EX31_RS00320 at 68.006 kb on + strand, within EX31_RS00320 at 68.007 kb on - strand, within EX31_RS00320 at 68.007 kb on - strand, within EX31_RS00320 at 68.008 kb on + strand, within EX31_RS00320 at 68.008 kb on + strand, within EX31_RS00320 at 68.008 kb on + strand, within EX31_RS00320 at 68.008 kb on + strand, within EX31_RS00320 at 68.009 kb on - strand, within EX31_RS00320 at 68.009 kb on - strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.019 kb on + strand, within EX31_RS00320 at 68.020 kb on - strand, within EX31_RS00320 at 68.020 kb on - strand, within EX31_RS00320 at 68.020 kb on - strand, within EX31_RS00320 at 68.020 kb on - strand, within EX31_RS00320 at 68.067 kb on + strand, within EX31_RS00320 at 68.079 kb on + strand, within EX31_RS00320 at 68.080 kb on - strand, within EX31_RS00320 at 68.107 kb on + strand, within EX31_RS00320 at 68.108 kb on - strand, within EX31_RS00320 at 68.112 kb on + strand, within EX31_RS00320 at 68.128 kb on + strand, within EX31_RS00320 at 68.129 kb on - strand, within EX31_RS00320 at 68.129 kb on - strand, within EX31_RS00320 at 68.129 kb on - strand, within EX31_RS00320 at 68.242 kb on + strand at 68.330 kb on + strand at 68.330 kb on + strand at 68.330 kb on + strand at 68.330 kb on + strand at 68.331 kb on - strand at 68.331 kb on - strand at 68.331 kb on - strand at 68.331 kb on - strand at 68.368 kb on - strand at 68.447 kb on - strand, within EX31_RS00325 at 68.502 kb on + strand, within EX31_RS00325 at 68.502 kb on + strand, within EX31_RS00325 at 68.502 kb on + strand, within EX31_RS00325 at 68.502 kb on + strand, within EX31_RS00325 at 68.502 kb on + strand, within EX31_RS00325 at 68.502 kb on + strand, within EX31_RS00325 at 68.502 kb on + strand, within EX31_RS00325 at 68.503 kb on - strand, within EX31_RS00325 at 68.503 kb on - strand, within EX31_RS00325 at 68.503 kb on - strand, within EX31_RS00325 at 68.503 kb on - strand, within EX31_RS00325 at 68.503 kb on - strand, within EX31_RS00325
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); ShewMM media; no shaking remove 66,130 - EX31_RS00310 0.11 -0.6 66,224 - EX31_RS00310 0.22 -0.4 66,224 - EX31_RS00310 0.22 -0.6 66,267 + EX31_RS00310 0.27 -0.1 66,267 + EX31_RS00310 0.27 +1.3 66,268 - EX31_RS00310 0.27 -0.6 66,268 - EX31_RS00310 0.27 +0.7 66,361 + EX31_RS00310 0.37 +0.7 66,361 + EX31_RS00310 0.37 -0.7 66,361 + EX31_RS00310 0.37 -0.1 66,361 + EX31_RS00310 0.37 -2.3 66,362 - EX31_RS00310 0.38 +0.9 66,362 - EX31_RS00310 0.38 +0.0 66,488 - EX31_RS00310 0.52 -0.2 66,530 - EX31_RS00310 0.57 +1.6 66,549 + EX31_RS00310 0.59 -0.9 66,623 + EX31_RS00310 0.67 -0.1 66,623 + EX31_RS00310 0.67 +0.4 66,623 + EX31_RS00310 0.67 -1.1 66,623 + EX31_RS00310 0.67 +1.8 66,624 - EX31_RS00310 0.67 -0.3 66,624 - EX31_RS00310 0.67 +0.2 66,624 - EX31_RS00310 0.67 -0.1 66,624 - EX31_RS00310 0.67 -0.3 66,624 - EX31_RS00310 0.67 +0.1 66,624 - EX31_RS00310 0.67 +0.3 66,624 - EX31_RS00310 0.67 -0.9 66,624 - EX31_RS00310 0.67 -0.1 66,628 + EX31_RS00310 0.68 +0.5 66,629 - EX31_RS00310 0.68 +1.3 66,629 - EX31_RS00310 0.68 -0.1 66,629 - EX31_RS00310 0.68 -0.2 66,629 - EX31_RS00310 0.68 +0.2 66,724 + EX31_RS00310 0.79 +0.5 66,725 - EX31_RS00310 0.79 +0.6 66,773 - EX31_RS00310 0.84 +0.3 66,904 + -0.6 66,904 + -0.3 66,904 + +0.0 66,904 + +1.3 66,904 + -0.9 66,904 + -2.1 66,905 - +0.6 66,905 - -0.6 66,905 - +0.3 66,905 - -1.2 66,905 - -0.2 66,905 - +2.0 66,905 - -0.7 66,905 - +0.7 66,905 - +1.3 66,905 - +0.4 66,915 + -0.3 66,915 + -0.3 67,019 + -0.6 67,020 - -0.0 67,020 - +0.5 67,023 + -0.6 67,023 + -0.9 67,023 + -0.6 67,023 + +0.3 67,023 + +0.8 67,023 + -0.2 67,023 + -0.7 67,023 + +0.3 67,023 + -1.3 67,023 + -0.7 67,023 + -0.3 67,023 + +2.0 67,023 + +1.3 67,023 + -0.6 67,024 - -0.2 67,024 - +0.7 67,024 - +0.6 67,024 - +0.3 67,024 - +1.0 67,024 - +0.4 67,024 - +0.8 67,024 - -1.2 67,024 - +0.3 67,024 - -0.1 67,024 - +0.5 67,024 - -2.1 67,024 - +0.1 67,024 - -0.4 67,024 - -0.2 67,024 - +1.5 67,024 - -1.1 67,024 - -0.3 67,024 - +0.2 67,024 - +0.4 67,027 + -0.5 67,027 + -0.6 67,028 - +0.5 67,058 + -1.6 67,058 + +1.1 67,058 + +1.0 67,059 - +0.7 67,059 - -1.3 67,059 - -0.5 67,059 - +0.2 67,152 - EX31_RS00315 0.25 -1.0 67,242 - EX31_RS00315 0.43 -1.0 67,242 - EX31_RS00315 0.43 -1.7 67,242 - EX31_RS00315 0.43 -0.1 67,242 - EX31_RS00315 0.43 +0.1 67,335 - EX31_RS00315 0.62 -0.7 67,419 + EX31_RS00315 0.78 -1.0 67,747 + EX31_RS00320 0.24 -1.1 67,803 + EX31_RS00320 0.32 +0.6 67,803 + EX31_RS00320 0.32 -0.9 67,803 + EX31_RS00320 0.32 +0.7 67,803 + EX31_RS00320 0.32 +1.3 67,837 + EX31_RS00320 0.37 +1.3 67,837 + EX31_RS00320 0.37 -2.0 67,837 + EX31_RS00320 0.37 -0.3 67,886 + EX31_RS00320 0.44 -0.2 67,896 + EX31_RS00320 0.45 -0.3 67,897 - EX31_RS00320 0.46 +0.4 67,910 - EX31_RS00320 0.47 -0.4 67,954 + EX31_RS00320 0.54 +2.3 67,955 - EX31_RS00320 0.54 +0.5 67,978 + EX31_RS00320 0.57 +0.0 68,006 + EX31_RS00320 0.61 +0.8 68,006 + EX31_RS00320 0.61 -0.1 68,006 + EX31_RS00320 0.61 +0.2 68,006 + EX31_RS00320 0.61 -0.4 68,007 - EX31_RS00320 0.61 +1.3 68,007 - EX31_RS00320 0.61 +1.3 68,008 + EX31_RS00320 0.62 -1.0 68,008 + EX31_RS00320 0.62 +1.3 68,008 + EX31_RS00320 0.62 -1.3 68,008 + EX31_RS00320 0.62 +2.3 68,009 - EX31_RS00320 0.62 +2.3 68,009 - EX31_RS00320 0.62 +0.6 68,019 + EX31_RS00320 0.63 +0.7 68,019 + EX31_RS00320 0.63 +0.9 68,019 + EX31_RS00320 0.63 +0.7 68,019 + EX31_RS00320 0.63 -0.4 68,019 + EX31_RS00320 0.63 -1.7 68,019 + EX31_RS00320 0.63 -0.2 68,019 + EX31_RS00320 0.63 +0.7 68,019 + EX31_RS00320 0.63 +0.3 68,019 + EX31_RS00320 0.63 -0.1 68,019 + EX31_RS00320 0.63 -3.2 68,020 - EX31_RS00320 0.63 +0.1 68,020 - EX31_RS00320 0.63 +0.6 68,020 - EX31_RS00320 0.63 +1.5 68,020 - EX31_RS00320 0.63 +0.8 68,067 + EX31_RS00320 0.70 +0.6 68,079 + EX31_RS00320 0.72 -0.6 68,080 - EX31_RS00320 0.72 -0.7 68,107 + EX31_RS00320 0.76 +1.6 68,108 - EX31_RS00320 0.76 +0.4 68,112 + EX31_RS00320 0.77 -1.0 68,128 + EX31_RS00320 0.79 +0.4 68,129 - EX31_RS00320 0.79 +0.3 68,129 - EX31_RS00320 0.79 +0.6 68,129 - EX31_RS00320 0.79 +0.1 68,242 + -0.3 68,330 + -1.3 68,330 + +0.2 68,330 + +0.2 68,330 + +0.2 68,331 - +0.2 68,331 - +0.9 68,331 - -0.2 68,331 - -1.1 68,368 - +1.3 68,447 - EX31_RS00325 0.17 +0.7 68,502 + EX31_RS00325 0.25 -1.1 68,502 + EX31_RS00325 0.25 -0.3 68,502 + EX31_RS00325 0.25 -1.3 68,502 + EX31_RS00325 0.25 -0.1 68,502 + EX31_RS00325 0.25 -0.4 68,502 + EX31_RS00325 0.25 +1.3 68,502 + EX31_RS00325 0.25 -0.6 68,503 - EX31_RS00325 0.25 +1.3 68,503 - EX31_RS00325 0.25 +0.4 68,503 - EX31_RS00325 0.25 +0.3 68,503 - EX31_RS00325 0.25 +0.8 68,503 - EX31_RS00325 0.25 +1.2
Or see this region's nucleotide sequence