Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20800 and EX31_RS25585 are separated by 89 nucleotides EX31_RS25585 and EX31_RS20805 are separated by 265 nucleotides EX31_RS20805 and EX31_RS20810 are separated by 62 nucleotides
EX31_RS20800: EX31_RS20800 - PAS domain-containing protein, at 809,584 to 811,134
_RS20800
EX31_RS25585: EX31_RS25585 - DUF333 domain-containing protein, at 811,224 to 811,457
_RS25585
EX31_RS20805: EX31_RS20805 - hypothetical protein, at 811,723 to 811,950
_RS20805
EX31_RS20810: EX31_RS20810 - DUF1471 domain-containing protein, at 812,013 to 812,270
_RS20810
Position (kb) 811
812 Strain fitness (log2 ratio) -2
-1
0
1
2 at 810.283 kb on + strand, within EX31_RS20800 at 810.284 kb on - strand, within EX31_RS20800 at 810.294 kb on + strand, within EX31_RS20800 at 810.403 kb on - strand, within EX31_RS20800 at 810.612 kb on + strand, within EX31_RS20800 at 810.612 kb on + strand, within EX31_RS20800 at 810.612 kb on + strand, within EX31_RS20800 at 810.837 kb on + strand, within EX31_RS20800 at 810.838 kb on - strand, within EX31_RS20800 at 810.838 kb on - strand, within EX31_RS20800 at 810.864 kb on + strand, within EX31_RS20800 at 810.864 kb on + strand, within EX31_RS20800 at 810.865 kb on - strand, within EX31_RS20800 at 810.865 kb on - strand, within EX31_RS20800 at 811.073 kb on - strand at 811.073 kb on - strand at 811.073 kb on - strand at 811.108 kb on + strand at 811.132 kb on + strand at 811.132 kb on + strand at 811.225 kb on + strand at 811.225 kb on + strand at 811.225 kb on + strand at 811.226 kb on - strand at 811.226 kb on - strand at 811.226 kb on - strand at 811.229 kb on + strand at 811.229 kb on + strand at 811.243 kb on + strand at 811.244 kb on - strand at 811.244 kb on - strand at 811.245 kb on + strand at 811.245 kb on + strand at 811.245 kb on + strand at 811.245 kb on + strand at 811.245 kb on + strand at 811.245 kb on + strand at 811.246 kb on - strand at 811.246 kb on - strand at 811.282 kb on - strand, within EX31_RS25585 at 811.282 kb on - strand, within EX31_RS25585 at 811.348 kb on + strand, within EX31_RS25585 at 811.348 kb on + strand, within EX31_RS25585 at 811.349 kb on - strand, within EX31_RS25585 at 811.349 kb on - strand, within EX31_RS25585 at 811.349 kb on - strand, within EX31_RS25585 at 811.350 kb on + strand, within EX31_RS25585 at 811.350 kb on + strand, within EX31_RS25585 at 811.394 kb on + strand, within EX31_RS25585 at 811.395 kb on - strand, within EX31_RS25585 at 811.395 kb on - strand, within EX31_RS25585 at 811.395 kb on - strand, within EX31_RS25585 at 811.402 kb on + strand, within EX31_RS25585 at 811.437 kb on - strand at 811.437 kb on - strand at 811.437 kb on - strand at 811.437 kb on - strand at 811.523 kb on - strand at 811.530 kb on + strand at 811.555 kb on - strand at 811.647 kb on + strand at 811.648 kb on - strand at 811.677 kb on - strand at 811.679 kb on + strand at 811.679 kb on + strand at 811.679 kb on + strand at 811.680 kb on - strand at 811.680 kb on - strand at 811.680 kb on - strand at 811.680 kb on - strand at 811.683 kb on - strand at 811.683 kb on - strand at 811.733 kb on - strand at 811.776 kb on + strand, within EX31_RS20805 at 811.776 kb on + strand, within EX31_RS20805 at 811.777 kb on - strand, within EX31_RS20805 at 811.777 kb on - strand, within EX31_RS20805 at 811.791 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.819 kb on + strand, within EX31_RS20805 at 811.820 kb on - strand, within EX31_RS20805 at 811.820 kb on - strand, within EX31_RS20805 at 811.820 kb on - strand, within EX31_RS20805 at 811.820 kb on - strand, within EX31_RS20805 at 811.820 kb on - strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.825 kb on + strand, within EX31_RS20805 at 811.826 kb on - strand, within EX31_RS20805 at 811.826 kb on - strand, within EX31_RS20805 at 811.826 kb on - strand, within EX31_RS20805 at 811.826 kb on - strand, within EX31_RS20805 at 811.826 kb on - strand, within EX31_RS20805 at 811.827 kb on + strand, within EX31_RS20805 at 811.827 kb on + strand, within EX31_RS20805 at 811.827 kb on + strand, within EX31_RS20805 at 811.827 kb on + strand, within EX31_RS20805 at 811.828 kb on - strand, within EX31_RS20805 at 811.828 kb on - strand, within EX31_RS20805 at 811.863 kb on + strand, within EX31_RS20805 at 811.863 kb on + strand, within EX31_RS20805 at 811.863 kb on + strand, within EX31_RS20805 at 811.863 kb on + strand, within EX31_RS20805 at 811.863 kb on + strand, within EX31_RS20805 at 811.864 kb on - strand, within EX31_RS20805 at 811.864 kb on - strand, within EX31_RS20805 at 811.864 kb on - strand, within EX31_RS20805 at 811.864 kb on - strand, within EX31_RS20805 at 811.864 kb on - strand, within EX31_RS20805 at 811.903 kb on + strand, within EX31_RS20805 at 811.903 kb on + strand, within EX31_RS20805 at 811.903 kb on + strand, within EX31_RS20805 at 811.903 kb on + strand, within EX31_RS20805 at 811.903 kb on + strand, within EX31_RS20805 at 811.904 kb on - strand, within EX31_RS20805 at 811.904 kb on - strand, within EX31_RS20805 at 811.904 kb on - strand, within EX31_RS20805 at 811.904 kb on - strand, within EX31_RS20805 at 811.904 kb on - strand, within EX31_RS20805 at 811.904 kb on - strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.905 kb on + strand, within EX31_RS20805 at 811.906 kb on - strand, within EX31_RS20805 at 811.906 kb on - strand, within EX31_RS20805 at 811.906 kb on - strand, within EX31_RS20805 at 811.906 kb on - strand, within EX31_RS20805 at 811.927 kb on - strand, within EX31_RS20805 at 811.938 kb on + strand at 811.938 kb on + strand at 811.938 kb on + strand at 811.939 kb on - strand at 811.939 kb on - strand at 811.939 kb on - strand at 811.992 kb on + strand at 811.992 kb on + strand at 811.993 kb on - strand at 811.999 kb on + strand at 812.000 kb on - strand at 812.000 kb on - strand at 812.000 kb on - strand at 812.002 kb on + strand at 812.002 kb on + strand at 812.014 kb on + strand at 812.015 kb on - strand at 812.015 kb on - strand at 812.015 kb on - strand at 812.021 kb on - strand at 812.038 kb on + strand at 812.039 kb on - strand, within EX31_RS20810 at 812.049 kb on - strand, within EX31_RS20810 at 812.069 kb on + strand, within EX31_RS20810 at 812.069 kb on + strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.070 kb on - strand, within EX31_RS20810 at 812.074 kb on + strand, within EX31_RS20810 at 812.157 kb on + strand, within EX31_RS20810 at 812.329 kb on + strand at 812.345 kb on + strand at 812.348 kb on + strand at 812.367 kb on - strand at 812.371 kb on - strand at 812.373 kb on + strand at 812.373 kb on + strand at 812.373 kb on + strand at 812.374 kb on - strand at 812.374 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 810,283 + EX31_RS20800 0.45 -0.9 810,284 - EX31_RS20800 0.45 +1.0 810,294 + EX31_RS20800 0.46 +0.9 810,403 - EX31_RS20800 0.53 -1.8 810,612 + EX31_RS20800 0.66 -1.1 810,612 + EX31_RS20800 0.66 +0.6 810,612 + EX31_RS20800 0.66 +0.8 810,837 + EX31_RS20800 0.81 +0.1 810,838 - EX31_RS20800 0.81 +0.5 810,838 - EX31_RS20800 0.81 -0.5 810,864 + EX31_RS20800 0.83 -0.7 810,864 + EX31_RS20800 0.83 -0.4 810,865 - EX31_RS20800 0.83 +1.7 810,865 - EX31_RS20800 0.83 +1.1 811,073 - -0.1 811,073 - -0.3 811,073 - +0.4 811,108 + -0.4 811,132 + -0.4 811,132 + +0.1 811,225 + -0.5 811,225 + +0.9 811,225 + +1.9 811,226 - -0.4 811,226 - -0.2 811,226 - +0.5 811,229 + -0.4 811,229 + -0.7 811,243 + +0.8 811,244 - -0.5 811,244 - -0.3 811,245 + -0.9 811,245 + +0.9 811,245 + +0.3 811,245 + -0.5 811,245 + +1.0 811,245 + +0.6 811,246 - +0.7 811,246 - -0.2 811,282 - EX31_RS25585 0.25 +0.6 811,282 - EX31_RS25585 0.25 -1.0 811,348 + EX31_RS25585 0.53 +0.4 811,348 + EX31_RS25585 0.53 -0.2 811,349 - EX31_RS25585 0.53 -0.2 811,349 - EX31_RS25585 0.53 -0.5 811,349 - EX31_RS25585 0.53 -1.4 811,350 + EX31_RS25585 0.54 -0.6 811,350 + EX31_RS25585 0.54 +0.8 811,394 + EX31_RS25585 0.73 +0.2 811,395 - EX31_RS25585 0.73 -2.1 811,395 - EX31_RS25585 0.73 -0.1 811,395 - EX31_RS25585 0.73 +0.1 811,402 + EX31_RS25585 0.76 +0.2 811,437 - +0.2 811,437 - +0.9 811,437 - -0.1 811,437 - +1.1 811,523 - -0.6 811,530 + +0.8 811,555 - -0.3 811,647 + +0.4 811,648 - -0.1 811,677 - +0.3 811,679 + +0.5 811,679 + +1.2 811,679 + -0.2 811,680 - +0.7 811,680 - -0.9 811,680 - +0.9 811,680 - -0.9 811,683 - +0.5 811,683 - +0.7 811,733 - +0.6 811,776 + EX31_RS20805 0.23 -2.0 811,776 + EX31_RS20805 0.23 +2.1 811,777 - EX31_RS20805 0.24 -0.5 811,777 - EX31_RS20805 0.24 +0.2 811,791 + EX31_RS20805 0.30 +1.1 811,819 + EX31_RS20805 0.42 -0.8 811,819 + EX31_RS20805 0.42 -0.0 811,819 + EX31_RS20805 0.42 +0.9 811,819 + EX31_RS20805 0.42 +0.0 811,819 + EX31_RS20805 0.42 +0.2 811,819 + EX31_RS20805 0.42 +1.3 811,819 + EX31_RS20805 0.42 -0.9 811,819 + EX31_RS20805 0.42 +0.7 811,820 - EX31_RS20805 0.43 +1.7 811,820 - EX31_RS20805 0.43 +2.1 811,820 - EX31_RS20805 0.43 -1.2 811,820 - EX31_RS20805 0.43 -0.1 811,820 - EX31_RS20805 0.43 -0.2 811,825 + EX31_RS20805 0.45 +0.3 811,825 + EX31_RS20805 0.45 -0.1 811,825 + EX31_RS20805 0.45 +0.9 811,825 + EX31_RS20805 0.45 -0.9 811,825 + EX31_RS20805 0.45 +0.9 811,825 + EX31_RS20805 0.45 -0.5 811,825 + EX31_RS20805 0.45 -1.9 811,825 + EX31_RS20805 0.45 -0.8 811,825 + EX31_RS20805 0.45 +1.1 811,825 + EX31_RS20805 0.45 -0.0 811,825 + EX31_RS20805 0.45 +0.1 811,825 + EX31_RS20805 0.45 +0.1 811,825 + EX31_RS20805 0.45 +0.1 811,826 - EX31_RS20805 0.45 +0.5 811,826 - EX31_RS20805 0.45 +0.8 811,826 - EX31_RS20805 0.45 -1.2 811,826 - EX31_RS20805 0.45 -0.2 811,826 - EX31_RS20805 0.45 -0.7 811,827 + EX31_RS20805 0.46 +0.3 811,827 + EX31_RS20805 0.46 -1.2 811,827 + EX31_RS20805 0.46 -0.6 811,827 + EX31_RS20805 0.46 -0.7 811,828 - EX31_RS20805 0.46 +0.3 811,828 - EX31_RS20805 0.46 -0.6 811,863 + EX31_RS20805 0.61 +0.1 811,863 + EX31_RS20805 0.61 -0.9 811,863 + EX31_RS20805 0.61 -0.8 811,863 + EX31_RS20805 0.61 +0.2 811,863 + EX31_RS20805 0.61 -0.1 811,864 - EX31_RS20805 0.62 +1.0 811,864 - EX31_RS20805 0.62 +0.5 811,864 - EX31_RS20805 0.62 -0.3 811,864 - EX31_RS20805 0.62 -0.9 811,864 - EX31_RS20805 0.62 +0.1 811,903 + EX31_RS20805 0.79 -0.3 811,903 + EX31_RS20805 0.79 -0.3 811,903 + EX31_RS20805 0.79 -0.3 811,903 + EX31_RS20805 0.79 -1.0 811,903 + EX31_RS20805 0.79 +0.2 811,904 - EX31_RS20805 0.79 +0.9 811,904 - EX31_RS20805 0.79 -1.2 811,904 - EX31_RS20805 0.79 -2.0 811,904 - EX31_RS20805 0.79 +0.8 811,904 - EX31_RS20805 0.79 +0.1 811,904 - EX31_RS20805 0.79 -0.8 811,905 + EX31_RS20805 0.80 -0.6 811,905 + EX31_RS20805 0.80 +1.6 811,905 + EX31_RS20805 0.80 -0.1 811,905 + EX31_RS20805 0.80 +0.8 811,905 + EX31_RS20805 0.80 -1.4 811,905 + EX31_RS20805 0.80 -1.0 811,905 + EX31_RS20805 0.80 +0.2 811,905 + EX31_RS20805 0.80 -0.4 811,905 + EX31_RS20805 0.80 -1.0 811,906 - EX31_RS20805 0.80 +0.5 811,906 - EX31_RS20805 0.80 -0.5 811,906 - EX31_RS20805 0.80 +0.7 811,906 - EX31_RS20805 0.80 +0.0 811,927 - EX31_RS20805 0.89 +2.5 811,938 + -0.4 811,938 + -0.4 811,938 + -0.9 811,939 - -0.7 811,939 - -0.2 811,939 - -1.2 811,992 + -0.5 811,992 + +0.2 811,993 - +0.5 811,999 + +1.1 812,000 - +0.2 812,000 - +0.8 812,000 - -0.3 812,002 + -1.2 812,002 + -1.1 812,014 + +0.4 812,015 - -0.3 812,015 - -0.3 812,015 - +0.6 812,021 - +0.4 812,038 + -0.4 812,039 - EX31_RS20810 0.10 +0.8 812,049 - EX31_RS20810 0.14 -1.8 812,069 + EX31_RS20810 0.22 -0.1 812,069 + EX31_RS20810 0.22 -1.2 812,070 - EX31_RS20810 0.22 -0.2 812,070 - EX31_RS20810 0.22 -0.2 812,070 - EX31_RS20810 0.22 +0.2 812,070 - EX31_RS20810 0.22 +0.6 812,070 - EX31_RS20810 0.22 +0.1 812,070 - EX31_RS20810 0.22 +0.5 812,070 - EX31_RS20810 0.22 -0.1 812,070 - EX31_RS20810 0.22 -0.4 812,070 - EX31_RS20810 0.22 +1.0 812,074 + EX31_RS20810 0.24 +0.1 812,157 + EX31_RS20810 0.56 +0.9 812,329 + +0.4 812,345 + -0.4 812,348 + +0.5 812,367 - -1.0 812,371 - +0.4 812,373 + +0.1 812,373 + +1.1 812,373 + +0.9 812,374 - -0.7 812,374 - +0.2
Or see this region's nucleotide sequence