Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18510 and EX31_RS18515 are separated by 106 nucleotides EX31_RS18515 and EX31_RS18520 are separated by 7 nucleotides EX31_RS18520 and EX31_RS18525 are separated by 194 nucleotides
EX31_RS18510: EX31_RS18510 - serine--tRNA ligase, at 273,576 to 274,868
_RS18510
EX31_RS18515: EX31_RS18515 - replication-associated recombination protein A, at 274,975 to 276,318
_RS18515
EX31_RS18520: EX31_RS18520 - outer membrane lipoprotein chaperone LolA, at 276,326 to 276,937
_RS18520
EX31_RS18525: EX31_RS18525 - DNA translocase FtsK, at 277,132 to 280,578
_RS18525
Position (kb)
274
275
276
277 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 274.946 kb on - strand at 274.946 kb on - strand at 274.960 kb on - strand at 274.977 kb on - strand at 275.072 kb on + strand at 275.072 kb on + strand at 275.072 kb on + strand at 275.072 kb on + strand at 275.073 kb on - strand at 275.073 kb on - strand at 275.073 kb on - strand at 275.073 kb on - strand at 275.073 kb on - strand at 275.075 kb on + strand at 275.076 kb on - strand at 275.105 kb on + strand at 275.106 kb on - strand at 275.106 kb on - strand at 275.106 kb on - strand at 275.106 kb on - strand at 275.124 kb on - strand, within EX31_RS18515 at 275.124 kb on - strand, within EX31_RS18515 at 275.124 kb on - strand, within EX31_RS18515 at 275.200 kb on - strand, within EX31_RS18515 at 275.200 kb on - strand, within EX31_RS18515 at 275.270 kb on + strand, within EX31_RS18515 at 275.270 kb on + strand, within EX31_RS18515 at 275.271 kb on - strand, within EX31_RS18515 at 275.272 kb on + strand, within EX31_RS18515 at 275.273 kb on - strand, within EX31_RS18515 at 275.273 kb on - strand, within EX31_RS18515 at 275.280 kb on + strand, within EX31_RS18515 at 275.280 kb on + strand, within EX31_RS18515 at 275.281 kb on - strand, within EX31_RS18515 at 275.281 kb on - strand, within EX31_RS18515 at 275.281 kb on - strand, within EX31_RS18515 at 275.281 kb on - strand, within EX31_RS18515 at 275.304 kb on - strand, within EX31_RS18515 at 275.304 kb on - strand, within EX31_RS18515 at 275.356 kb on - strand, within EX31_RS18515 at 275.356 kb on - strand, within EX31_RS18515 at 275.450 kb on + strand, within EX31_RS18515 at 275.451 kb on - strand, within EX31_RS18515 at 275.451 kb on - strand, within EX31_RS18515 at 275.451 kb on - strand, within EX31_RS18515 at 275.451 kb on - strand, within EX31_RS18515 at 275.492 kb on + strand, within EX31_RS18515 at 275.493 kb on - strand, within EX31_RS18515 at 275.493 kb on - strand, within EX31_RS18515 at 275.529 kb on + strand, within EX31_RS18515 at 275.530 kb on - strand, within EX31_RS18515 at 275.553 kb on - strand, within EX31_RS18515 at 275.568 kb on + strand, within EX31_RS18515 at 275.612 kb on - strand, within EX31_RS18515 at 275.832 kb on + strand, within EX31_RS18515 at 275.832 kb on + strand, within EX31_RS18515 at 275.836 kb on + strand, within EX31_RS18515 at 275.837 kb on - strand, within EX31_RS18515 at 275.837 kb on - strand, within EX31_RS18515 at 275.837 kb on - strand, within EX31_RS18515 at 275.837 kb on - strand, within EX31_RS18515 at 275.837 kb on - strand, within EX31_RS18515 at 275.840 kb on + strand, within EX31_RS18515 at 275.840 kb on + strand, within EX31_RS18515 at 275.840 kb on + strand, within EX31_RS18515 at 275.841 kb on - strand, within EX31_RS18515 at 275.841 kb on - strand, within EX31_RS18515 at 275.841 kb on - strand, within EX31_RS18515 at 275.913 kb on + strand, within EX31_RS18515 at 275.913 kb on + strand, within EX31_RS18515 at 275.913 kb on + strand, within EX31_RS18515 at 275.913 kb on + strand, within EX31_RS18515 at 275.914 kb on - strand, within EX31_RS18515 at 275.914 kb on - strand, within EX31_RS18515 at 276.055 kb on - strand, within EX31_RS18515 at 276.077 kb on - strand, within EX31_RS18515 at 276.077 kb on - strand, within EX31_RS18515 at 276.077 kb on - strand, within EX31_RS18515 at 276.078 kb on + strand, within EX31_RS18515 at 276.078 kb on + strand, within EX31_RS18515 at 276.078 kb on + strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.079 kb on - strand, within EX31_RS18515 at 276.234 kb on + strand at 276.234 kb on + strand at 276.234 kb on + strand at 276.234 kb on + strand at 276.235 kb on - strand at 276.235 kb on - strand at 276.235 kb on - strand at 276.236 kb on + strand at 276.236 kb on + strand at 276.236 kb on + strand at 276.237 kb on - strand at 276.237 kb on - strand at 276.312 kb on + strand at 276.322 kb on - strand at 276.322 kb on - strand at 276.322 kb on - strand at 276.322 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.948 kb on - strand at 276.984 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.001 kb on - strand at 277.066 kb on + strand at 277.105 kb on + strand at 277.295 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 274,946 - -2.9 274,946 - +0.9 274,960 - -3.0 274,977 - -1.1 275,072 + -0.2 275,072 + -0.5 275,072 + -0.6 275,072 + -0.1 275,073 - +1.1 275,073 - -2.9 275,073 - -0.4 275,073 - +1.1 275,073 - +1.0 275,075 + -0.4 275,076 - -0.4 275,105 + +0.5 275,106 - +0.0 275,106 - -0.9 275,106 - -0.5 275,106 - -1.6 275,124 - EX31_RS18515 0.11 -1.0 275,124 - EX31_RS18515 0.11 -1.2 275,124 - EX31_RS18515 0.11 +1.4 275,200 - EX31_RS18515 0.17 +0.9 275,200 - EX31_RS18515 0.17 +0.4 275,270 + EX31_RS18515 0.22 -1.3 275,270 + EX31_RS18515 0.22 -1.3 275,271 - EX31_RS18515 0.22 -0.3 275,272 + EX31_RS18515 0.22 +0.2 275,273 - EX31_RS18515 0.22 +0.4 275,273 - EX31_RS18515 0.22 +0.0 275,280 + EX31_RS18515 0.23 -1.1 275,280 + EX31_RS18515 0.23 +0.9 275,281 - EX31_RS18515 0.23 +0.2 275,281 - EX31_RS18515 0.23 +0.4 275,281 - EX31_RS18515 0.23 +0.9 275,281 - EX31_RS18515 0.23 -2.1 275,304 - EX31_RS18515 0.24 +0.5 275,304 - EX31_RS18515 0.24 +0.4 275,356 - EX31_RS18515 0.28 -0.6 275,356 - EX31_RS18515 0.28 +0.4 275,450 + EX31_RS18515 0.35 +1.2 275,451 - EX31_RS18515 0.35 -0.6 275,451 - EX31_RS18515 0.35 +0.0 275,451 - EX31_RS18515 0.35 +3.3 275,451 - EX31_RS18515 0.35 -0.2 275,492 + EX31_RS18515 0.38 +1.2 275,493 - EX31_RS18515 0.39 -0.1 275,493 - EX31_RS18515 0.39 +0.7 275,529 + EX31_RS18515 0.41 +0.7 275,530 - EX31_RS18515 0.41 -0.0 275,553 - EX31_RS18515 0.43 -2.1 275,568 + EX31_RS18515 0.44 -0.2 275,612 - EX31_RS18515 0.47 -0.9 275,832 + EX31_RS18515 0.64 +3.5 275,832 + EX31_RS18515 0.64 -1.0 275,836 + EX31_RS18515 0.64 +0.4 275,837 - EX31_RS18515 0.64 -0.2 275,837 - EX31_RS18515 0.64 -0.8 275,837 - EX31_RS18515 0.64 +0.4 275,837 - EX31_RS18515 0.64 -0.4 275,837 - EX31_RS18515 0.64 -0.4 275,840 + EX31_RS18515 0.64 +1.1 275,840 + EX31_RS18515 0.64 +1.6 275,840 + EX31_RS18515 0.64 -0.6 275,841 - EX31_RS18515 0.64 -2.3 275,841 - EX31_RS18515 0.64 +0.0 275,841 - EX31_RS18515 0.64 -0.1 275,913 + EX31_RS18515 0.70 -1.1 275,913 + EX31_RS18515 0.70 -2.1 275,913 + EX31_RS18515 0.70 -0.2 275,913 + EX31_RS18515 0.70 +0.2 275,914 - EX31_RS18515 0.70 +0.9 275,914 - EX31_RS18515 0.70 +1.9 276,055 - EX31_RS18515 0.80 -0.4 276,077 - EX31_RS18515 0.82 -1.2 276,077 - EX31_RS18515 0.82 -0.4 276,077 - EX31_RS18515 0.82 -1.2 276,078 + EX31_RS18515 0.82 -0.2 276,078 + EX31_RS18515 0.82 -0.8 276,078 + EX31_RS18515 0.82 -0.4 276,079 - EX31_RS18515 0.82 -0.8 276,079 - EX31_RS18515 0.82 -0.3 276,079 - EX31_RS18515 0.82 +0.6 276,079 - EX31_RS18515 0.82 +0.3 276,079 - EX31_RS18515 0.82 +0.4 276,079 - EX31_RS18515 0.82 +1.4 276,079 - EX31_RS18515 0.82 +0.2 276,079 - EX31_RS18515 0.82 +0.0 276,079 - EX31_RS18515 0.82 +0.4 276,079 - EX31_RS18515 0.82 -0.7 276,079 - EX31_RS18515 0.82 -0.1 276,079 - EX31_RS18515 0.82 -0.2 276,079 - EX31_RS18515 0.82 +1.8 276,234 + +1.5 276,234 + -0.2 276,234 + -1.2 276,234 + +0.8 276,235 - -0.0 276,235 - -0.1 276,235 - -1.1 276,236 + -0.4 276,236 + -1.8 276,236 + -0.9 276,237 - -0.1 276,237 - +0.4 276,312 + +0.4 276,322 - +0.9 276,322 - +0.0 276,322 - -1.1 276,322 - -1.8 276,948 - +1.0 276,948 - +0.7 276,948 - +0.2 276,948 - -1.0 276,948 - -0.4 276,948 - +0.9 276,948 - -0.6 276,948 - +0.6 276,984 - +0.3 277,001 - +3.5 277,001 - +0.2 277,001 - +0.1 277,001 - +0.1 277,001 - +0.1 277,001 - +1.2 277,001 - +0.4 277,066 + +0.7 277,105 + -0.1 277,295 - -1.5
Or see this region's nucleotide sequence