Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18085 and EX31_RS18090 are separated by 30 nucleotides EX31_RS18090 and EX31_RS18095 are separated by 184 nucleotides EX31_RS18095 and EX31_RS18100 are separated by 315 nucleotides EX31_RS18100 and EX31_RS18105 are separated by 18 nucleotides
EX31_RS18085: EX31_RS18085 - MOSC domain-containing protein, at 178,731 to 179,834
_RS18085
EX31_RS18090: EX31_RS18090 - cell division protein ZapC, at 179,865 to 180,413
_RS18090
EX31_RS18095: EX31_RS18095 - quinone-dependent dihydroorotate dehydrogenase, at 180,598 to 181,608
_RS18095
EX31_RS18100: EX31_RS18100 - NADPH-dependent FMN reductase, at 181,924 to 182,505
_RS18100
EX31_RS18105: EX31_RS18105 - sulfonate ABC transporter substrate-binding protein, at 182,524 to 183,498
_RS18105
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 179.606 kb on + strand, within EX31_RS18085 at 179.606 kb on + strand, within EX31_RS18085 at 179.606 kb on + strand, within EX31_RS18085 at 179.607 kb on - strand, within EX31_RS18085 at 179.607 kb on - strand, within EX31_RS18085 at 179.677 kb on + strand, within EX31_RS18085 at 179.677 kb on + strand, within EX31_RS18085 at 179.677 kb on + strand, within EX31_RS18085 at 179.677 kb on + strand, within EX31_RS18085 at 179.677 kb on + strand, within EX31_RS18085 at 179.677 kb on + strand, within EX31_RS18085 at 179.678 kb on - strand, within EX31_RS18085 at 179.678 kb on - strand, within EX31_RS18085 at 179.681 kb on + strand, within EX31_RS18085 at 179.681 kb on + strand, within EX31_RS18085 at 179.788 kb on + strand at 179.788 kb on + strand at 179.788 kb on + strand at 179.789 kb on - strand at 179.791 kb on + strand at 179.798 kb on + strand at 179.798 kb on + strand at 179.798 kb on + strand at 179.798 kb on + strand at 179.798 kb on + strand at 179.798 kb on + strand at 179.799 kb on - strand at 179.799 kb on - strand at 179.799 kb on - strand at 179.799 kb on - strand at 179.799 kb on - strand at 179.804 kb on + strand at 179.832 kb on + strand at 179.840 kb on + strand at 179.840 kb on + strand at 179.840 kb on + strand at 179.840 kb on + strand at 179.841 kb on - strand at 179.841 kb on - strand at 179.841 kb on - strand at 179.841 kb on - strand at 179.841 kb on - strand at 179.844 kb on + strand at 179.844 kb on + strand at 179.867 kb on - strand at 179.881 kb on + strand at 179.939 kb on + strand, within EX31_RS18090 at 179.940 kb on - strand, within EX31_RS18090 at 179.943 kb on - strand, within EX31_RS18090 at 180.014 kb on + strand, within EX31_RS18090 at 180.015 kb on - strand, within EX31_RS18090 at 180.037 kb on - strand, within EX31_RS18090 at 180.037 kb on - strand, within EX31_RS18090 at 180.215 kb on + strand, within EX31_RS18090 at 180.245 kb on - strand, within EX31_RS18090 at 180.276 kb on - strand, within EX31_RS18090 at 180.354 kb on - strand, within EX31_RS18090 at 180.354 kb on - strand, within EX31_RS18090 at 180.372 kb on - strand at 180.380 kb on - strand at 180.380 kb on - strand at 180.393 kb on - strand at 180.471 kb on - strand at 180.544 kb on + strand at 180.561 kb on + strand at 180.561 kb on + strand at 180.562 kb on - strand at 180.562 kb on - strand at 180.562 kb on - strand at 180.562 kb on - strand at 180.565 kb on + strand at 180.565 kb on + strand at 180.599 kb on + strand at 180.600 kb on - strand at 180.603 kb on + strand at 180.604 kb on - strand at 180.604 kb on - strand at 180.615 kb on + strand at 180.616 kb on - strand at 180.616 kb on - strand at 180.616 kb on - strand at 180.667 kb on + strand at 180.668 kb on - strand at 180.668 kb on - strand at 180.751 kb on + strand, within EX31_RS18095 at 180.751 kb on + strand, within EX31_RS18095 at 180.871 kb on - strand, within EX31_RS18095 at 180.898 kb on - strand, within EX31_RS18095 at 180.978 kb on - strand, within EX31_RS18095 at 180.978 kb on - strand, within EX31_RS18095 at 180.997 kb on + strand, within EX31_RS18095 at 180.998 kb on - strand, within EX31_RS18095 at 181.067 kb on - strand, within EX31_RS18095 at 181.105 kb on - strand, within EX31_RS18095 at 181.105 kb on - strand, within EX31_RS18095 at 181.123 kb on + strand, within EX31_RS18095 at 181.124 kb on - strand, within EX31_RS18095 at 181.124 kb on - strand, within EX31_RS18095 at 181.124 kb on - strand, within EX31_RS18095 at 181.134 kb on + strand, within EX31_RS18095 at 181.135 kb on - strand, within EX31_RS18095 at 181.135 kb on - strand, within EX31_RS18095 at 181.145 kb on + strand, within EX31_RS18095 at 181.173 kb on + strand, within EX31_RS18095 at 181.173 kb on + strand, within EX31_RS18095 at 181.180 kb on - strand, within EX31_RS18095 at 181.192 kb on - strand, within EX31_RS18095 at 181.195 kb on - strand, within EX31_RS18095 at 181.233 kb on + strand, within EX31_RS18095 at 181.234 kb on - strand, within EX31_RS18095 at 181.310 kb on - strand, within EX31_RS18095 at 181.320 kb on + strand, within EX31_RS18095 at 181.320 kb on + strand, within EX31_RS18095 at 181.398 kb on + strand, within EX31_RS18095 at 181.399 kb on - strand, within EX31_RS18095 at 181.399 kb on - strand, within EX31_RS18095 at 181.447 kb on - strand, within EX31_RS18095 at 181.546 kb on + strand at 181.604 kb on + strand at 181.604 kb on + strand at 181.604 kb on + strand at 181.604 kb on + strand at 181.605 kb on - strand at 181.605 kb on - strand at 181.634 kb on - strand at 181.646 kb on - strand at 181.655 kb on + strand at 181.667 kb on - strand at 181.686 kb on + strand at 181.689 kb on - strand at 181.734 kb on + strand at 181.751 kb on + strand at 181.797 kb on + strand at 181.797 kb on + strand at 181.798 kb on - strand at 181.858 kb on + strand at 181.889 kb on + strand at 181.895 kb on + strand at 181.895 kb on + strand at 181.897 kb on + strand at 181.911 kb on - strand at 181.931 kb on + strand at 182.029 kb on - strand, within EX31_RS18100 at 182.033 kb on - strand, within EX31_RS18100 at 182.033 kb on - strand, within EX31_RS18100 at 182.037 kb on + strand, within EX31_RS18100 at 182.037 kb on + strand, within EX31_RS18100 at 182.152 kb on + strand, within EX31_RS18100 at 182.152 kb on + strand, within EX31_RS18100 at 182.152 kb on + strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.153 kb on - strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.154 kb on + strand, within EX31_RS18100 at 182.253 kb on + strand, within EX31_RS18100 at 182.253 kb on + strand, within EX31_RS18100 at 182.254 kb on - strand, within EX31_RS18100 at 182.262 kb on + strand, within EX31_RS18100 at 182.363 kb on - strand, within EX31_RS18100 at 182.509 kb on + strand at 182.519 kb on + strand at 182.519 kb on + strand at 182.520 kb on - strand at 182.520 kb on - strand at 182.543 kb on + strand at 182.543 kb on + strand at 182.543 kb on + strand at 182.543 kb on + strand at 182.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 179,606 + EX31_RS18085 0.79 +0.7 179,606 + EX31_RS18085 0.79 +1.9 179,606 + EX31_RS18085 0.79 +0.3 179,607 - EX31_RS18085 0.79 +0.9 179,607 - EX31_RS18085 0.79 +1.9 179,677 + EX31_RS18085 0.86 +1.3 179,677 + EX31_RS18085 0.86 +0.2 179,677 + EX31_RS18085 0.86 +1.5 179,677 + EX31_RS18085 0.86 +0.5 179,677 + EX31_RS18085 0.86 -0.6 179,677 + EX31_RS18085 0.86 +0.7 179,678 - EX31_RS18085 0.86 -0.7 179,678 - EX31_RS18085 0.86 +1.3 179,681 + EX31_RS18085 0.86 -0.2 179,681 + EX31_RS18085 0.86 -0.1 179,788 + -0.0 179,788 + +1.0 179,788 + -0.4 179,789 - -0.4 179,791 + -0.1 179,798 + +0.7 179,798 + +0.6 179,798 + +0.3 179,798 + +1.0 179,798 + -0.4 179,798 + -0.1 179,799 - -1.1 179,799 - -0.8 179,799 - +0.4 179,799 - +0.7 179,799 - +1.9 179,804 + +0.5 179,832 + +0.1 179,840 + -1.0 179,840 + -1.9 179,840 + +1.5 179,840 + +0.1 179,841 - -0.3 179,841 - +0.9 179,841 - +0.1 179,841 - +0.9 179,841 - -1.2 179,844 + +0.9 179,844 + -1.5 179,867 - -0.4 179,881 + +0.3 179,939 + EX31_RS18090 0.13 -2.4 179,940 - EX31_RS18090 0.14 -0.2 179,943 - EX31_RS18090 0.14 -0.7 180,014 + EX31_RS18090 0.27 -0.7 180,015 - EX31_RS18090 0.27 +1.4 180,037 - EX31_RS18090 0.31 +0.9 180,037 - EX31_RS18090 0.31 +0.0 180,215 + EX31_RS18090 0.64 -0.4 180,245 - EX31_RS18090 0.69 -0.3 180,276 - EX31_RS18090 0.75 +0.3 180,354 - EX31_RS18090 0.89 -1.2 180,354 - EX31_RS18090 0.89 -0.7 180,372 - +0.8 180,380 - -0.6 180,380 - -0.2 180,393 - -0.5 180,471 - +1.5 180,544 + -1.4 180,561 + -0.1 180,561 + +0.3 180,562 - +0.1 180,562 - -0.5 180,562 - +0.9 180,562 - +0.8 180,565 + +0.8 180,565 + -0.3 180,599 + +2.7 180,600 - +0.3 180,603 + -2.7 180,604 - -1.4 180,604 - +0.1 180,615 + -2.2 180,616 - -2.0 180,616 - -1.9 180,616 - -2.2 180,667 + -2.2 180,668 - -0.7 180,668 - -1.2 180,751 + EX31_RS18095 0.15 -1.8 180,751 + EX31_RS18095 0.15 -2.8 180,871 - EX31_RS18095 0.27 -0.9 180,898 - EX31_RS18095 0.30 -0.4 180,978 - EX31_RS18095 0.38 -2.2 180,978 - EX31_RS18095 0.38 -3.2 180,997 + EX31_RS18095 0.39 -2.2 180,998 - EX31_RS18095 0.40 -0.7 181,067 - EX31_RS18095 0.46 -2.1 181,105 - EX31_RS18095 0.50 -0.4 181,105 - EX31_RS18095 0.50 -1.1 181,123 + EX31_RS18095 0.52 -1.2 181,124 - EX31_RS18095 0.52 -2.5 181,124 - EX31_RS18095 0.52 -2.0 181,124 - EX31_RS18095 0.52 -0.7 181,134 + EX31_RS18095 0.53 -2.4 181,135 - EX31_RS18095 0.53 -1.4 181,135 - EX31_RS18095 0.53 -0.7 181,145 + EX31_RS18095 0.54 +0.9 181,173 + EX31_RS18095 0.57 -2.4 181,173 + EX31_RS18095 0.57 -0.9 181,180 - EX31_RS18095 0.58 -1.4 181,192 - EX31_RS18095 0.59 -3.2 181,195 - EX31_RS18095 0.59 -2.9 181,233 + EX31_RS18095 0.63 -3.3 181,234 - EX31_RS18095 0.63 -1.4 181,310 - EX31_RS18095 0.70 -1.9 181,320 + EX31_RS18095 0.71 -0.9 181,320 + EX31_RS18095 0.71 -1.4 181,398 + EX31_RS18095 0.79 -1.9 181,399 - EX31_RS18095 0.79 -2.0 181,399 - EX31_RS18095 0.79 +0.3 181,447 - EX31_RS18095 0.84 -0.9 181,546 + -1.5 181,604 + -2.5 181,604 + -1.9 181,604 + -2.2 181,604 + -1.5 181,605 - -1.5 181,605 - -2.2 181,634 - -1.3 181,646 - +0.7 181,655 + -1.7 181,667 - -2.7 181,686 + +0.5 181,689 - -1.7 181,734 + -0.3 181,751 + +0.3 181,797 + +1.2 181,797 + +0.7 181,798 - +2.0 181,858 + -0.3 181,889 + +1.5 181,895 + +1.1 181,895 + -0.7 181,897 + +0.3 181,911 - -0.0 181,931 + +0.5 182,029 - EX31_RS18100 0.18 +0.1 182,033 - EX31_RS18100 0.19 -2.1 182,033 - EX31_RS18100 0.19 +0.5 182,037 + EX31_RS18100 0.19 +1.0 182,037 + EX31_RS18100 0.19 +0.4 182,152 + EX31_RS18100 0.39 -0.5 182,152 + EX31_RS18100 0.39 -0.4 182,152 + EX31_RS18100 0.39 +1.9 182,153 - EX31_RS18100 0.39 +1.9 182,153 - EX31_RS18100 0.39 +0.6 182,153 - EX31_RS18100 0.39 +1.0 182,153 - EX31_RS18100 0.39 +0.9 182,154 + EX31_RS18100 0.40 +0.6 182,154 + EX31_RS18100 0.40 -1.4 182,154 + EX31_RS18100 0.40 +1.9 182,154 + EX31_RS18100 0.40 -0.6 182,253 + EX31_RS18100 0.57 -0.4 182,253 + EX31_RS18100 0.57 +1.1 182,254 - EX31_RS18100 0.57 -0.4 182,262 + EX31_RS18100 0.58 +1.1 182,363 - EX31_RS18100 0.75 +0.1 182,509 + -0.3 182,519 + +1.7 182,519 + -1.5 182,520 - +0.6 182,520 - +0.5 182,543 + -0.3 182,543 + +0.1 182,543 + +1.9 182,543 + -0.8 182,547 - +1.3
Or see this region's nucleotide sequence