Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12525 and EX31_RS12530 are separated by 65 nucleotides EX31_RS12530 and EX31_RS12535 are separated by 9 nucleotides EX31_RS12535 and EX31_RS12540 are separated by 30 nucleotides EX31_RS12540 and EX31_RS12545 are separated by 65 nucleotides
EX31_RS12525: EX31_RS12525 - monofunctional biosynthetic peptidoglycan transglycosylase, at 176,880 to 177,602
_RS12525
EX31_RS12530: EX31_RS12530 - divisome-associated lipoprotein YraP, at 177,668 to 178,243
_RS12530
EX31_RS12535: EX31_RS12535 - DnaA initiator-associating protein DiaA, at 178,253 to 178,843
_RS12535
EX31_RS12540: EX31_RS12540 - YraN family protein, at 178,874 to 179,242
_RS12540
EX31_RS12545: EX31_RS12545 - penicillin-binding protein activator, at 179,308 to 181,344
_RS12545
Position (kb) 178
179 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 177.284 kb on + strand, within EX31_RS12525 at 177.284 kb on + strand, within EX31_RS12525 at 177.285 kb on - strand, within EX31_RS12525 at 177.354 kb on + strand, within EX31_RS12525 at 177.354 kb on + strand, within EX31_RS12525 at 177.354 kb on + strand, within EX31_RS12525 at 177.355 kb on - strand, within EX31_RS12525 at 177.355 kb on - strand, within EX31_RS12525 at 177.422 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.516 kb on + strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.517 kb on - strand, within EX31_RS12525 at 177.523 kb on + strand, within EX31_RS12525 at 177.523 kb on + strand, within EX31_RS12525 at 177.524 kb on - strand, within EX31_RS12525 at 177.524 kb on - strand, within EX31_RS12525 at 177.590 kb on + strand at 177.590 kb on + strand at 177.591 kb on - strand at 177.600 kb on + strand at 177.600 kb on + strand at 177.654 kb on + strand at 177.678 kb on + strand at 177.678 kb on + strand at 177.808 kb on + strand, within EX31_RS12530 at 177.809 kb on - strand, within EX31_RS12530 at 177.836 kb on + strand, within EX31_RS12530 at 177.868 kb on + strand, within EX31_RS12530 at 177.868 kb on + strand, within EX31_RS12530 at 177.869 kb on - strand, within EX31_RS12530 at 177.902 kb on - strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.921 kb on + strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 177.922 kb on - strand, within EX31_RS12530 at 178.012 kb on + strand, within EX31_RS12530 at 178.020 kb on + strand, within EX31_RS12530 at 178.066 kb on + strand, within EX31_RS12530 at 178.078 kb on + strand, within EX31_RS12530 at 178.078 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.126 kb on + strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.127 kb on - strand, within EX31_RS12530 at 178.196 kb on + strand at 178.196 kb on + strand at 178.196 kb on + strand at 178.196 kb on + strand at 178.197 kb on - strand at 178.197 kb on - strand at 178.197 kb on - strand at 178.197 kb on - strand at 178.212 kb on - strand at 178.212 kb on - strand at 178.217 kb on + strand at 178.217 kb on + strand at 178.218 kb on - strand at 178.218 kb on - strand at 178.255 kb on - strand at 178.282 kb on + strand at 178.282 kb on + strand at 178.283 kb on - strand at 178.286 kb on + strand at 178.287 kb on - strand at 178.306 kb on + strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.307 kb on - strand at 178.318 kb on + strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.319 kb on - strand, within EX31_RS12535 at 178.435 kb on + strand, within EX31_RS12535 at 178.472 kb on - strand, within EX31_RS12535 at 178.526 kb on + strand, within EX31_RS12535 at 178.544 kb on - strand, within EX31_RS12535 at 178.549 kb on - strand, within EX31_RS12535 at 178.549 kb on - strand, within EX31_RS12535 at 178.597 kb on + strand, within EX31_RS12535 at 178.597 kb on + strand, within EX31_RS12535 at 178.598 kb on - strand, within EX31_RS12535 at 178.598 kb on - strand, within EX31_RS12535 at 178.607 kb on + strand, within EX31_RS12535 at 178.607 kb on + strand, within EX31_RS12535 at 178.607 kb on + strand, within EX31_RS12535 at 178.607 kb on + strand, within EX31_RS12535 at 178.607 kb on + strand, within EX31_RS12535 at 178.607 kb on + strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.608 kb on - strand, within EX31_RS12535 at 178.628 kb on - strand, within EX31_RS12535 at 178.717 kb on + strand, within EX31_RS12535 at 178.808 kb on - strand at 178.868 kb on + strand at 178.869 kb on - strand at 178.871 kb on + strand at 178.871 kb on + strand at 178.872 kb on - strand at 178.872 kb on - strand at 178.872 kb on - strand at 178.875 kb on + strand at 178.876 kb on - strand at 178.907 kb on + strand at 178.907 kb on + strand at 178.907 kb on + strand at 178.907 kb on + strand at 178.908 kb on - strand at 178.908 kb on - strand at 178.908 kb on - strand at 178.908 kb on - strand at 178.908 kb on - strand at 178.908 kb on - strand at 179.199 kb on + strand, within EX31_RS12540 at 179.199 kb on + strand, within EX31_RS12540 at 179.199 kb on + strand, within EX31_RS12540 at 179.200 kb on - strand, within EX31_RS12540 at 179.200 kb on - strand, within EX31_RS12540 at 179.200 kb on - strand, within EX31_RS12540 at 179.200 kb on - strand, within EX31_RS12540 at 179.200 kb on - strand, within EX31_RS12540 at 179.246 kb on + strand at 179.246 kb on + strand at 179.247 kb on - strand at 179.247 kb on - strand at 179.247 kb on - strand at 179.247 kb on - strand at 179.256 kb on + strand at 179.256 kb on + strand at 179.256 kb on + strand at 179.256 kb on + strand at 179.256 kb on + strand at 179.257 kb on - strand at 179.257 kb on - strand at 179.257 kb on - strand at 179.257 kb on - strand at 179.262 kb on + strand at 179.263 kb on - strand at 179.263 kb on - strand at 179.309 kb on + strand at 179.361 kb on + strand at 179.361 kb on + strand at 179.362 kb on - strand at 179.362 kb on - strand at 179.382 kb on + strand at 179.487 kb on - strand at 179.504 kb on + strand at 179.505 kb on - strand at 179.606 kb on + strand, within EX31_RS12545 at 179.630 kb on + strand, within EX31_RS12545 at 179.631 kb on - strand, within EX31_RS12545 at 179.631 kb on - strand, within EX31_RS12545 at 179.631 kb on - strand, within EX31_RS12545 at 179.649 kb on - strand, within EX31_RS12545 at 179.732 kb on + strand, within EX31_RS12545 at 179.732 kb on + strand, within EX31_RS12545 at 179.732 kb on + strand, within EX31_RS12545 at 179.733 kb on - strand, within EX31_RS12545 at 179.733 kb on - strand, within EX31_RS12545 at 179.733 kb on - strand, within EX31_RS12545
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 177,284 + EX31_RS12525 0.56 +0.1 177,284 + EX31_RS12525 0.56 -1.4 177,285 - EX31_RS12525 0.56 -0.7 177,354 + EX31_RS12525 0.66 -0.2 177,354 + EX31_RS12525 0.66 +0.9 177,354 + EX31_RS12525 0.66 +0.7 177,355 - EX31_RS12525 0.66 +1.0 177,355 - EX31_RS12525 0.66 -0.5 177,422 + EX31_RS12525 0.75 -0.2 177,516 + EX31_RS12525 0.88 -0.1 177,516 + EX31_RS12525 0.88 -0.4 177,516 + EX31_RS12525 0.88 +0.6 177,516 + EX31_RS12525 0.88 +0.0 177,516 + EX31_RS12525 0.88 -0.0 177,517 - EX31_RS12525 0.88 +0.6 177,517 - EX31_RS12525 0.88 -1.5 177,517 - EX31_RS12525 0.88 +1.5 177,517 - EX31_RS12525 0.88 -0.5 177,517 - EX31_RS12525 0.88 +0.5 177,517 - EX31_RS12525 0.88 -0.1 177,517 - EX31_RS12525 0.88 -1.0 177,517 - EX31_RS12525 0.88 -1.7 177,517 - EX31_RS12525 0.88 -0.3 177,517 - EX31_RS12525 0.88 -0.0 177,523 + EX31_RS12525 0.89 +0.4 177,523 + EX31_RS12525 0.89 +0.6 177,524 - EX31_RS12525 0.89 +0.1 177,524 - EX31_RS12525 0.89 -0.3 177,590 + +0.9 177,590 + -0.4 177,591 - -0.0 177,600 + -1.0 177,600 + +0.2 177,654 + -1.0 177,678 + -0.4 177,678 + -1.3 177,808 + EX31_RS12530 0.24 +2.1 177,809 - EX31_RS12530 0.24 +0.9 177,836 + EX31_RS12530 0.29 -0.4 177,868 + EX31_RS12530 0.35 -0.4 177,868 + EX31_RS12530 0.35 +0.8 177,869 - EX31_RS12530 0.35 -0.7 177,902 - EX31_RS12530 0.41 -1.6 177,921 + EX31_RS12530 0.44 +0.8 177,921 + EX31_RS12530 0.44 +0.4 177,921 + EX31_RS12530 0.44 -1.2 177,921 + EX31_RS12530 0.44 +1.4 177,921 + EX31_RS12530 0.44 +0.3 177,922 - EX31_RS12530 0.44 -0.4 177,922 - EX31_RS12530 0.44 -0.3 177,922 - EX31_RS12530 0.44 +0.7 177,922 - EX31_RS12530 0.44 +1.0 177,922 - EX31_RS12530 0.44 +0.8 177,922 - EX31_RS12530 0.44 +0.4 177,922 - EX31_RS12530 0.44 +0.4 177,922 - EX31_RS12530 0.44 -0.6 178,012 + EX31_RS12530 0.60 +0.3 178,020 + EX31_RS12530 0.61 +0.6 178,066 + EX31_RS12530 0.69 +1.0 178,078 + EX31_RS12530 0.71 -1.6 178,078 + EX31_RS12530 0.71 +0.6 178,126 + EX31_RS12530 0.80 -0.5 178,126 + EX31_RS12530 0.80 +0.2 178,126 + EX31_RS12530 0.80 -0.2 178,126 + EX31_RS12530 0.80 +1.0 178,127 - EX31_RS12530 0.80 -0.3 178,127 - EX31_RS12530 0.80 -0.1 178,127 - EX31_RS12530 0.80 +0.6 178,127 - EX31_RS12530 0.80 +0.9 178,127 - EX31_RS12530 0.80 +0.6 178,196 + -0.9 178,196 + -1.0 178,196 + -1.1 178,196 + +0.6 178,197 - +0.1 178,197 - +0.5 178,197 - +0.9 178,197 - +1.0 178,212 - +0.5 178,212 - -0.8 178,217 + +0.3 178,217 + -0.3 178,218 - +0.0 178,218 - +1.0 178,255 - +0.8 178,282 + +0.6 178,282 + +1.8 178,283 - +0.7 178,286 + +2.2 178,287 - +0.8 178,306 + +2.2 178,307 - +0.0 178,307 - +0.8 178,307 - +1.1 178,307 - +0.4 178,307 - +0.4 178,307 - +1.4 178,318 + EX31_RS12535 0.11 +0.6 178,319 - EX31_RS12535 0.11 +0.6 178,319 - EX31_RS12535 0.11 +1.6 178,319 - EX31_RS12535 0.11 +1.4 178,319 - EX31_RS12535 0.11 +0.7 178,435 + EX31_RS12535 0.31 +0.3 178,472 - EX31_RS12535 0.37 +0.7 178,526 + EX31_RS12535 0.46 +1.4 178,544 - EX31_RS12535 0.49 +1.0 178,549 - EX31_RS12535 0.50 +1.5 178,549 - EX31_RS12535 0.50 +1.3 178,597 + EX31_RS12535 0.58 +0.2 178,597 + EX31_RS12535 0.58 +4.0 178,598 - EX31_RS12535 0.58 +2.2 178,598 - EX31_RS12535 0.58 +1.0 178,607 + EX31_RS12535 0.60 +0.6 178,607 + EX31_RS12535 0.60 +1.9 178,607 + EX31_RS12535 0.60 +2.0 178,607 + EX31_RS12535 0.60 +3.0 178,607 + EX31_RS12535 0.60 +1.0 178,607 + EX31_RS12535 0.60 +2.8 178,608 - EX31_RS12535 0.60 +2.2 178,608 - EX31_RS12535 0.60 +1.9 178,608 - EX31_RS12535 0.60 +1.8 178,608 - EX31_RS12535 0.60 +0.7 178,608 - EX31_RS12535 0.60 +2.2 178,608 - EX31_RS12535 0.60 +0.2 178,608 - EX31_RS12535 0.60 +2.0 178,608 - EX31_RS12535 0.60 +0.3 178,608 - EX31_RS12535 0.60 +1.8 178,628 - EX31_RS12535 0.63 +0.4 178,717 + EX31_RS12535 0.79 +0.3 178,808 - +2.1 178,868 + +0.2 178,869 - -1.5 178,871 + +1.2 178,871 + +1.7 178,872 - +1.3 178,872 - -0.7 178,872 - +0.5 178,875 + +1.5 178,876 - -0.4 178,907 + +1.6 178,907 + +0.8 178,907 + +1.7 178,907 + +1.0 178,908 - +0.6 178,908 - -0.5 178,908 - -0.7 178,908 - +1.7 178,908 - +0.0 178,908 - +0.5 179,199 + EX31_RS12540 0.88 +0.9 179,199 + EX31_RS12540 0.88 +0.2 179,199 + EX31_RS12540 0.88 +0.7 179,200 - EX31_RS12540 0.88 +0.4 179,200 - EX31_RS12540 0.88 +0.5 179,200 - EX31_RS12540 0.88 -0.0 179,200 - EX31_RS12540 0.88 +0.4 179,200 - EX31_RS12540 0.88 -0.1 179,246 + +1.5 179,246 + +2.0 179,247 - -0.6 179,247 - +0.3 179,247 - +0.7 179,247 - -0.4 179,256 + -1.0 179,256 + +0.3 179,256 + +1.2 179,256 + +1.2 179,256 + +0.6 179,257 - -0.5 179,257 - -0.3 179,257 - -0.3 179,257 - -0.6 179,262 + +0.8 179,263 - +0.0 179,263 - +0.2 179,309 + +1.3 179,361 + +0.9 179,361 + +0.7 179,362 - +0.1 179,362 - -0.1 179,382 + +0.1 179,487 - +0.5 179,504 + +0.6 179,505 - +0.2 179,606 + EX31_RS12545 0.15 +0.5 179,630 + EX31_RS12545 0.16 +2.6 179,631 - EX31_RS12545 0.16 +1.0 179,631 - EX31_RS12545 0.16 -0.8 179,631 - EX31_RS12545 0.16 -1.0 179,649 - EX31_RS12545 0.17 +0.4 179,732 + EX31_RS12545 0.21 -1.3 179,732 + EX31_RS12545 0.21 +0.5 179,732 + EX31_RS12545 0.21 -0.5 179,733 - EX31_RS12545 0.21 -0.3 179,733 - EX31_RS12545 0.21 -1.4 179,733 - EX31_RS12545 0.21 -0.6
Or see this region's nucleotide sequence