Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09765 and EX31_RS09770 are separated by 91 nucleotides EX31_RS09770 and EX31_RS09775 are separated by 197 nucleotides EX31_RS09775 and EX31_RS09780 are separated by 3 nucleotides
EX31_RS09765: EX31_RS09765 - xylose isomerase, at 158,181 to 159,500
_RS09765
EX31_RS09770: EX31_RS09770 - xylulokinase, at 159,592 to 161,043
_RS09770
EX31_RS09775: EX31_RS09775 - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 161,241 to 161,531
_RS09775
EX31_RS09780: EX31_RS09780 - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 161,535 to 162,410
_RS09780
Position (kb) 159
160
161
162 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 158.601 kb on - strand, within EX31_RS09765 at 158.601 kb on - strand, within EX31_RS09765 at 158.601 kb on - strand, within EX31_RS09765 at 158.654 kb on + strand, within EX31_RS09765 at 158.733 kb on + strand, within EX31_RS09765 at 158.733 kb on + strand, within EX31_RS09765 at 158.733 kb on + strand, within EX31_RS09765 at 158.778 kb on - strand, within EX31_RS09765 at 158.778 kb on - strand, within EX31_RS09765 at 158.880 kb on - strand, within EX31_RS09765 at 158.907 kb on + strand, within EX31_RS09765 at 158.907 kb on + strand, within EX31_RS09765 at 158.907 kb on + strand, within EX31_RS09765 at 158.907 kb on + strand, within EX31_RS09765 at 158.907 kb on + strand, within EX31_RS09765 at 158.908 kb on - strand, within EX31_RS09765 at 158.908 kb on - strand, within EX31_RS09765 at 158.913 kb on + strand, within EX31_RS09765 at 158.913 kb on + strand, within EX31_RS09765 at 158.913 kb on + strand, within EX31_RS09765 at 158.914 kb on - strand, within EX31_RS09765 at 158.931 kb on + strand, within EX31_RS09765 at 158.931 kb on + strand, within EX31_RS09765 at 159.147 kb on + strand, within EX31_RS09765 at 159.147 kb on + strand, within EX31_RS09765 at 159.147 kb on + strand, within EX31_RS09765 at 159.148 kb on - strand, within EX31_RS09765 at 159.148 kb on - strand, within EX31_RS09765 at 159.148 kb on - strand, within EX31_RS09765 at 159.228 kb on + strand, within EX31_RS09765 at 159.228 kb on + strand, within EX31_RS09765 at 159.228 kb on + strand, within EX31_RS09765 at 159.228 kb on + strand, within EX31_RS09765 at 159.228 kb on + strand, within EX31_RS09765 at 159.229 kb on - strand, within EX31_RS09765 at 159.229 kb on - strand, within EX31_RS09765 at 159.229 kb on - strand, within EX31_RS09765 at 159.229 kb on - strand, within EX31_RS09765 at 159.240 kb on + strand, within EX31_RS09765 at 159.339 kb on + strand, within EX31_RS09765 at 159.340 kb on - strand, within EX31_RS09765 at 159.411 kb on + strand at 159.511 kb on + strand at 159.580 kb on + strand at 159.591 kb on + strand at 159.591 kb on + strand at 159.591 kb on + strand at 159.591 kb on + strand at 159.591 kb on + strand at 159.592 kb on - strand at 159.592 kb on - strand at 159.592 kb on - strand at 159.596 kb on - strand at 159.605 kb on + strand at 159.638 kb on + strand at 159.794 kb on + strand, within EX31_RS09770 at 159.795 kb on - strand, within EX31_RS09770 at 159.795 kb on - strand, within EX31_RS09770 at 159.857 kb on + strand, within EX31_RS09770 at 159.860 kb on + strand, within EX31_RS09770 at 159.860 kb on + strand, within EX31_RS09770 at 159.861 kb on - strand, within EX31_RS09770 at 160.054 kb on + strand, within EX31_RS09770 at 160.055 kb on - strand, within EX31_RS09770 at 160.092 kb on + strand, within EX31_RS09770 at 160.092 kb on + strand, within EX31_RS09770 at 160.093 kb on - strand, within EX31_RS09770 at 160.093 kb on - strand, within EX31_RS09770 at 160.132 kb on - strand, within EX31_RS09770 at 160.323 kb on + strand, within EX31_RS09770 at 160.324 kb on - strand, within EX31_RS09770 at 160.344 kb on - strand, within EX31_RS09770 at 160.367 kb on - strand, within EX31_RS09770 at 160.585 kb on + strand, within EX31_RS09770 at 160.586 kb on - strand, within EX31_RS09770 at 160.611 kb on + strand, within EX31_RS09770 at 160.685 kb on + strand, within EX31_RS09770 at 160.685 kb on + strand, within EX31_RS09770 at 160.883 kb on + strand, within EX31_RS09770 at 160.883 kb on + strand, within EX31_RS09770 at 160.952 kb on + strand at 160.952 kb on + strand at 160.952 kb on + strand at 160.953 kb on - strand at 160.977 kb on + strand at 160.978 kb on - strand at 160.984 kb on + strand at 160.984 kb on + strand at 161.015 kb on + strand at 161.015 kb on + strand at 161.015 kb on + strand at 161.017 kb on + strand at 161.017 kb on + strand at 161.017 kb on + strand at 161.017 kb on + strand at 161.017 kb on + strand at 161.017 kb on + strand at 161.017 kb on + strand at 161.018 kb on - strand at 161.018 kb on - strand at 161.018 kb on - strand at 161.018 kb on - strand at 161.018 kb on - strand at 161.018 kb on - strand at 161.018 kb on - strand at 161.041 kb on + strand at 161.041 kb on + strand at 161.041 kb on + strand at 161.045 kb on - strand at 161.045 kb on - strand at 161.045 kb on - strand at 161.188 kb on + strand at 161.188 kb on + strand at 161.192 kb on - strand at 161.237 kb on + strand at 161.251 kb on - strand at 161.258 kb on + strand at 161.289 kb on - strand, within EX31_RS09775 at 161.321 kb on - strand, within EX31_RS09775 at 161.366 kb on - strand, within EX31_RS09775 at 161.375 kb on - strand, within EX31_RS09775 at 161.470 kb on - strand, within EX31_RS09775 at 161.563 kb on + strand at 161.564 kb on - strand at 161.794 kb on + strand, within EX31_RS09780 at 161.794 kb on + strand, within EX31_RS09780 at 161.798 kb on - strand, within EX31_RS09780 at 161.798 kb on - strand, within EX31_RS09780 at 161.798 kb on - strand, within EX31_RS09780 at 161.855 kb on - strand, within EX31_RS09780 at 161.968 kb on + strand, within EX31_RS09780 at 161.968 kb on + strand, within EX31_RS09780 at 161.969 kb on - strand, within EX31_RS09780 at 161.983 kb on + strand, within EX31_RS09780 at 161.983 kb on + strand, within EX31_RS09780 at 161.983 kb on + strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780 at 161.984 kb on - strand, within EX31_RS09780
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 158,601 - EX31_RS09765 0.32 +0.8 158,601 - EX31_RS09765 0.32 -2.3 158,601 - EX31_RS09765 0.32 -0.6 158,654 + EX31_RS09765 0.36 -1.1 158,733 + EX31_RS09765 0.42 +2.2 158,733 + EX31_RS09765 0.42 -1.3 158,733 + EX31_RS09765 0.42 +1.7 158,778 - EX31_RS09765 0.45 +0.4 158,778 - EX31_RS09765 0.45 +0.6 158,880 - EX31_RS09765 0.53 +0.8 158,907 + EX31_RS09765 0.55 -0.6 158,907 + EX31_RS09765 0.55 +1.1 158,907 + EX31_RS09765 0.55 -0.2 158,907 + EX31_RS09765 0.55 +0.9 158,907 + EX31_RS09765 0.55 -0.5 158,908 - EX31_RS09765 0.55 +1.2 158,908 - EX31_RS09765 0.55 +0.7 158,913 + EX31_RS09765 0.55 +2.0 158,913 + EX31_RS09765 0.55 -0.0 158,913 + EX31_RS09765 0.55 +1.1 158,914 - EX31_RS09765 0.56 +1.8 158,931 + EX31_RS09765 0.57 +1.8 158,931 + EX31_RS09765 0.57 +0.0 159,147 + EX31_RS09765 0.73 -0.3 159,147 + EX31_RS09765 0.73 +0.4 159,147 + EX31_RS09765 0.73 +0.8 159,148 - EX31_RS09765 0.73 +2.8 159,148 - EX31_RS09765 0.73 -0.2 159,148 - EX31_RS09765 0.73 -0.5 159,228 + EX31_RS09765 0.79 -0.0 159,228 + EX31_RS09765 0.79 +0.5 159,228 + EX31_RS09765 0.79 -0.0 159,228 + EX31_RS09765 0.79 -0.0 159,228 + EX31_RS09765 0.79 +0.0 159,229 - EX31_RS09765 0.79 +2.1 159,229 - EX31_RS09765 0.79 +0.8 159,229 - EX31_RS09765 0.79 +0.5 159,229 - EX31_RS09765 0.79 +1.1 159,240 + EX31_RS09765 0.80 +1.0 159,339 + EX31_RS09765 0.88 +0.2 159,340 - EX31_RS09765 0.88 +1.1 159,411 + -0.8 159,511 + +0.2 159,580 + +0.2 159,591 + -1.0 159,591 + -0.2 159,591 + +1.4 159,591 + -0.5 159,591 + +1.5 159,592 - +1.0 159,592 - +1.1 159,592 - -0.0 159,596 - +0.7 159,605 + +0.0 159,638 + +1.2 159,794 + EX31_RS09770 0.14 +0.2 159,795 - EX31_RS09770 0.14 -0.3 159,795 - EX31_RS09770 0.14 -0.2 159,857 + EX31_RS09770 0.18 -0.9 159,860 + EX31_RS09770 0.18 +0.9 159,860 + EX31_RS09770 0.18 -0.5 159,861 - EX31_RS09770 0.19 +0.2 160,054 + EX31_RS09770 0.32 +1.5 160,055 - EX31_RS09770 0.32 -0.8 160,092 + EX31_RS09770 0.34 +0.1 160,092 + EX31_RS09770 0.34 +0.8 160,093 - EX31_RS09770 0.35 -0.2 160,093 - EX31_RS09770 0.35 -0.5 160,132 - EX31_RS09770 0.37 +0.7 160,323 + EX31_RS09770 0.50 +0.2 160,324 - EX31_RS09770 0.50 +0.8 160,344 - EX31_RS09770 0.52 -1.6 160,367 - EX31_RS09770 0.53 -0.1 160,585 + EX31_RS09770 0.68 -2.3 160,586 - EX31_RS09770 0.68 -0.5 160,611 + EX31_RS09770 0.70 -1.1 160,685 + EX31_RS09770 0.75 -0.3 160,685 + EX31_RS09770 0.75 -1.8 160,883 + EX31_RS09770 0.89 +0.5 160,883 + EX31_RS09770 0.89 -0.5 160,952 + +1.4 160,952 + +1.1 160,952 + +1.2 160,953 - -1.5 160,977 + +2.8 160,978 - -0.3 160,984 + +0.6 160,984 + +0.7 161,015 + +0.6 161,015 + -0.4 161,015 + -0.1 161,017 + +1.2 161,017 + -1.2 161,017 + -0.3 161,017 + +0.4 161,017 + +1.5 161,017 + +1.0 161,017 + +2.2 161,018 - +1.8 161,018 - +2.0 161,018 - -0.2 161,018 - +1.0 161,018 - -0.3 161,018 - -0.0 161,018 - +2.4 161,041 + +0.5 161,041 + +0.8 161,041 + +1.7 161,045 - +0.6 161,045 - +0.1 161,045 - -0.3 161,188 + -2.0 161,188 + +0.5 161,192 - +0.8 161,237 + +0.2 161,251 - +0.4 161,258 + -0.5 161,289 - EX31_RS09775 0.16 -0.3 161,321 - EX31_RS09775 0.27 +0.8 161,366 - EX31_RS09775 0.43 -1.3 161,375 - EX31_RS09775 0.46 +0.8 161,470 - EX31_RS09775 0.79 -0.9 161,563 + +0.0 161,564 - -0.3 161,794 + EX31_RS09780 0.30 +0.8 161,794 + EX31_RS09780 0.30 +2.2 161,798 - EX31_RS09780 0.30 -0.6 161,798 - EX31_RS09780 0.30 +2.4 161,798 - EX31_RS09780 0.30 +2.4 161,855 - EX31_RS09780 0.37 +1.1 161,968 + EX31_RS09780 0.49 +2.8 161,968 + EX31_RS09780 0.49 +0.2 161,969 - EX31_RS09780 0.50 +0.9 161,983 + EX31_RS09780 0.51 +0.5 161,983 + EX31_RS09780 0.51 +0.8 161,983 + EX31_RS09780 0.51 +2.8 161,984 - EX31_RS09780 0.51 +0.7 161,984 - EX31_RS09780 0.51 +0.9 161,984 - EX31_RS09780 0.51 -1.0 161,984 - EX31_RS09780 0.51 -0.6 161,984 - EX31_RS09780 0.51 +1.8 161,984 - EX31_RS09780 0.51 +0.0 161,984 - EX31_RS09780 0.51 -3.2 161,984 - EX31_RS09780 0.51 +0.7
Or see this region's nucleotide sequence