Strain Fitness in Rahnella sp. WP5 around EX31_RS09565

Experiment: L-Glutamine (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS09555 and EX31_RS09560 are separated by 21 nucleotidesEX31_RS09560 and EX31_RS09565 overlap by 4 nucleotidesEX31_RS09565 and EX31_RS09570 are separated by 208 nucleotidesEX31_RS09570 and EX31_RS09575 are separated by 7 nucleotides EX31_RS09555: EX31_RS09555 - flagellum-specific ATP synthase FliI, at 115,557 to 116,921 _RS09555 EX31_RS09560: EX31_RS09560 - flagella biosynthesis chaperone FliJ, at 116,943 to 117,389 _RS09560 EX31_RS09565: EX31_RS09565 - flagellar hook-length control protein FliK, at 117,386 to 118,774 _RS09565 EX31_RS09570: EX31_RS09570 - flagellar basal body-associated protein FliL, at 118,983 to 119,483 _RS09570 EX31_RS09575: EX31_RS09575 - flagellar motor switch protein FliM, at 119,491 to 120,513 _RS09575 Position (kb) 117 118 119Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 116.443 kb on - strand, within EX31_RS09555at 116.530 kb on - strand, within EX31_RS09555at 116.530 kb on - strand, within EX31_RS09555at 116.536 kb on + strand, within EX31_RS09555at 116.536 kb on + strand, within EX31_RS09555at 116.537 kb on - strand, within EX31_RS09555at 116.690 kb on + strand, within EX31_RS09555at 116.745 kb on + strand, within EX31_RS09555at 116.746 kb on - strand, within EX31_RS09555at 116.781 kb on + strand, within EX31_RS09555at 116.877 kb on + strandat 116.877 kb on + strandat 116.877 kb on + strandat 116.877 kb on + strandat 116.877 kb on + strandat 116.877 kb on + strandat 116.877 kb on + strandat 116.878 kb on - strandat 116.878 kb on - strandat 116.878 kb on - strandat 116.878 kb on - strandat 116.878 kb on - strandat 116.930 kb on - strandat 116.940 kb on + strandat 117.075 kb on + strand, within EX31_RS09560at 117.075 kb on + strand, within EX31_RS09560at 117.087 kb on + strand, within EX31_RS09560at 117.202 kb on + strand, within EX31_RS09560at 117.202 kb on + strand, within EX31_RS09560at 117.202 kb on + strand, within EX31_RS09560at 117.274 kb on - strand, within EX31_RS09560at 117.464 kb on - strandat 117.466 kb on + strandat 117.467 kb on - strandat 117.583 kb on + strand, within EX31_RS09565at 118.439 kb on - strand, within EX31_RS09565at 118.690 kb on + strandat 118.690 kb on + strandat 118.691 kb on - strandat 118.809 kb on + strandat 118.858 kb on + strandat 118.858 kb on + strandat 118.859 kb on - strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.959 kb on + strandat 118.963 kb on + strandat 119.047 kb on + strand, within EX31_RS09570at 119.047 kb on + strand, within EX31_RS09570at 119.186 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.238 kb on + strand, within EX31_RS09570at 119.239 kb on - strand, within EX31_RS09570at 119.239 kb on - strand, within EX31_RS09570at 119.239 kb on - strand, within EX31_RS09570at 119.282 kb on + strand, within EX31_RS09570at 119.296 kb on - strand, within EX31_RS09570at 119.490 kb on + strandat 119.491 kb on - strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.504 kb on + strandat 119.505 kb on - strandat 119.609 kb on + strand, within EX31_RS09575at 119.748 kb on - strand, within EX31_RS09575

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking
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116,443 - EX31_RS09555 0.65 -0.6
116,530 - EX31_RS09555 0.71 +1.8
116,530 - EX31_RS09555 0.71 -0.2
116,536 + EX31_RS09555 0.72 -1.5
116,536 + EX31_RS09555 0.72 -1.9
116,537 - EX31_RS09555 0.72 -1.8
116,690 + EX31_RS09555 0.83 -1.9
116,745 + EX31_RS09555 0.87 -0.8
116,746 - EX31_RS09555 0.87 -1.0
116,781 + EX31_RS09555 0.90 -2.4
116,877 + -0.5
116,877 + +0.1
116,877 + -1.6
116,877 + -0.4
116,877 + +0.4
116,877 + -0.9
116,877 + +1.8
116,878 - +0.8
116,878 - -1.6
116,878 - -1.4
116,878 - +0.8
116,878 - -0.5
116,930 - -0.8
116,940 + +1.4
117,075 + EX31_RS09560 0.30 +1.0
117,075 + EX31_RS09560 0.30 -1.5
117,087 + EX31_RS09560 0.32 -0.3
117,202 + EX31_RS09560 0.58 -2.4
117,202 + EX31_RS09560 0.58 -0.8
117,202 + EX31_RS09560 0.58 -0.3
117,274 - EX31_RS09560 0.74 -1.8
117,464 - +0.6
117,466 + +0.1
117,467 - -0.8
117,583 + EX31_RS09565 0.14 -1.1
118,439 - EX31_RS09565 0.76 +0.2
118,690 + -0.4
118,690 + +0.1
118,691 - -0.6
118,809 + +0.1
118,858 + -0.6
118,858 + +0.7
118,859 - +0.0
118,959 + +1.0
118,959 + -1.8
118,959 + -0.4
118,959 + +0.4
118,959 + -3.0
118,963 + -1.9
119,047 + EX31_RS09570 0.13 +0.0
119,047 + EX31_RS09570 0.13 -0.3
119,186 + EX31_RS09570 0.41 -0.6
119,238 + EX31_RS09570 0.51 -0.5
119,238 + EX31_RS09570 0.51 -0.6
119,238 + EX31_RS09570 0.51 -1.2
119,238 + EX31_RS09570 0.51 -0.6
119,238 + EX31_RS09570 0.51 -1.0
119,239 - EX31_RS09570 0.51 +0.1
119,239 - EX31_RS09570 0.51 +1.8
119,239 - EX31_RS09570 0.51 -1.2
119,282 + EX31_RS09570 0.60 -0.7
119,296 - EX31_RS09570 0.62 -0.2
119,490 + -1.2
119,491 - -0.6
119,504 + -1.3
119,504 + +0.2
119,504 + -0.0
119,504 + +1.0
119,504 + -1.8
119,505 - +0.8
119,609 + EX31_RS09575 0.12 -0.0
119,748 - EX31_RS09575 0.25 -0.5

Or see this region's nucleotide sequence