Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09555 and EX31_RS09560 are separated by 21 nucleotides EX31_RS09560 and EX31_RS09565 overlap by 4 nucleotides EX31_RS09565 and EX31_RS09570 are separated by 208 nucleotides EX31_RS09570 and EX31_RS09575 are separated by 7 nucleotides
EX31_RS09555: EX31_RS09555 - flagellum-specific ATP synthase FliI, at 115,557 to 116,921
_RS09555
EX31_RS09560: EX31_RS09560 - flagella biosynthesis chaperone FliJ, at 116,943 to 117,389
_RS09560
EX31_RS09565: EX31_RS09565 - flagellar hook-length control protein FliK, at 117,386 to 118,774
_RS09565
EX31_RS09570: EX31_RS09570 - flagellar basal body-associated protein FliL, at 118,983 to 119,483
_RS09570
EX31_RS09575: EX31_RS09575 - flagellar motor switch protein FliM, at 119,491 to 120,513
_RS09575
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 116.443 kb on - strand, within EX31_RS09555 at 116.530 kb on - strand, within EX31_RS09555 at 116.530 kb on - strand, within EX31_RS09555 at 116.536 kb on + strand, within EX31_RS09555 at 116.536 kb on + strand, within EX31_RS09555 at 116.537 kb on - strand, within EX31_RS09555 at 116.690 kb on + strand, within EX31_RS09555 at 116.745 kb on + strand, within EX31_RS09555 at 116.746 kb on - strand, within EX31_RS09555 at 116.781 kb on + strand, within EX31_RS09555 at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.877 kb on + strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.878 kb on - strand at 116.930 kb on - strand at 116.940 kb on + strand at 117.075 kb on + strand, within EX31_RS09560 at 117.075 kb on + strand, within EX31_RS09560 at 117.087 kb on + strand, within EX31_RS09560 at 117.202 kb on + strand, within EX31_RS09560 at 117.202 kb on + strand, within EX31_RS09560 at 117.202 kb on + strand, within EX31_RS09560 at 117.274 kb on - strand, within EX31_RS09560 at 117.464 kb on - strand at 117.466 kb on + strand at 117.467 kb on - strand at 117.583 kb on + strand, within EX31_RS09565 at 118.439 kb on - strand, within EX31_RS09565 at 118.690 kb on + strand at 118.690 kb on + strand at 118.691 kb on - strand at 118.809 kb on + strand at 118.858 kb on + strand at 118.858 kb on + strand at 118.859 kb on - strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.959 kb on + strand at 118.963 kb on + strand at 119.047 kb on + strand, within EX31_RS09570 at 119.047 kb on + strand, within EX31_RS09570 at 119.186 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.238 kb on + strand, within EX31_RS09570 at 119.239 kb on - strand, within EX31_RS09570 at 119.239 kb on - strand, within EX31_RS09570 at 119.239 kb on - strand, within EX31_RS09570 at 119.282 kb on + strand, within EX31_RS09570 at 119.296 kb on - strand, within EX31_RS09570 at 119.490 kb on + strand at 119.491 kb on - strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.504 kb on + strand at 119.505 kb on - strand at 119.609 kb on + strand, within EX31_RS09575 at 119.748 kb on - strand, within EX31_RS09575
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 116,443 - EX31_RS09555 0.65 -0.6 116,530 - EX31_RS09555 0.71 +1.8 116,530 - EX31_RS09555 0.71 -0.2 116,536 + EX31_RS09555 0.72 -1.5 116,536 + EX31_RS09555 0.72 -1.9 116,537 - EX31_RS09555 0.72 -1.8 116,690 + EX31_RS09555 0.83 -1.9 116,745 + EX31_RS09555 0.87 -0.8 116,746 - EX31_RS09555 0.87 -1.0 116,781 + EX31_RS09555 0.90 -2.4 116,877 + -0.5 116,877 + +0.1 116,877 + -1.6 116,877 + -0.4 116,877 + +0.4 116,877 + -0.9 116,877 + +1.8 116,878 - +0.8 116,878 - -1.6 116,878 - -1.4 116,878 - +0.8 116,878 - -0.5 116,930 - -0.8 116,940 + +1.4 117,075 + EX31_RS09560 0.30 +1.0 117,075 + EX31_RS09560 0.30 -1.5 117,087 + EX31_RS09560 0.32 -0.3 117,202 + EX31_RS09560 0.58 -2.4 117,202 + EX31_RS09560 0.58 -0.8 117,202 + EX31_RS09560 0.58 -0.3 117,274 - EX31_RS09560 0.74 -1.8 117,464 - +0.6 117,466 + +0.1 117,467 - -0.8 117,583 + EX31_RS09565 0.14 -1.1 118,439 - EX31_RS09565 0.76 +0.2 118,690 + -0.4 118,690 + +0.1 118,691 - -0.6 118,809 + +0.1 118,858 + -0.6 118,858 + +0.7 118,859 - +0.0 118,959 + +1.0 118,959 + -1.8 118,959 + -0.4 118,959 + +0.4 118,959 + -3.0 118,963 + -1.9 119,047 + EX31_RS09570 0.13 +0.0 119,047 + EX31_RS09570 0.13 -0.3 119,186 + EX31_RS09570 0.41 -0.6 119,238 + EX31_RS09570 0.51 -0.5 119,238 + EX31_RS09570 0.51 -0.6 119,238 + EX31_RS09570 0.51 -1.2 119,238 + EX31_RS09570 0.51 -0.6 119,238 + EX31_RS09570 0.51 -1.0 119,239 - EX31_RS09570 0.51 +0.1 119,239 - EX31_RS09570 0.51 +1.8 119,239 - EX31_RS09570 0.51 -1.2 119,282 + EX31_RS09570 0.60 -0.7 119,296 - EX31_RS09570 0.62 -0.2 119,490 + -1.2 119,491 - -0.6 119,504 + -1.3 119,504 + +0.2 119,504 + -0.0 119,504 + +1.0 119,504 + -1.8 119,505 - +0.8 119,609 + EX31_RS09575 0.12 -0.0 119,748 - EX31_RS09575 0.25 -0.5
Or see this region's nucleotide sequence