Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS08025 and EX31_RS08030 are separated by 216 nucleotides EX31_RS08030 and EX31_RS08035 are separated by 9 nucleotides
EX31_RS08025: EX31_RS08025 - cystathionine beta-lyase, at 95,328 to 96,521
_RS08025
EX31_RS08030: EX31_RS08030 - DedA family protein, at 96,738 to 97,397
_RS08030
EX31_RS08035: EX31_RS08035 - AraC family transcriptional regulator, at 97,407 to 98,342
_RS08035
Position (kb) 96
97
98 Strain fitness (log2 ratio) -2
-1
0
1
2 at 95.746 kb on + strand, within EX31_RS08025 at 95.909 kb on + strand, within EX31_RS08025 at 95.933 kb on + strand, within EX31_RS08025 at 95.934 kb on - strand, within EX31_RS08025 at 95.934 kb on - strand, within EX31_RS08025 at 95.934 kb on - strand, within EX31_RS08025 at 95.958 kb on + strand, within EX31_RS08025 at 95.958 kb on + strand, within EX31_RS08025 at 95.958 kb on + strand, within EX31_RS08025 at 95.958 kb on + strand, within EX31_RS08025 at 95.958 kb on + strand, within EX31_RS08025 at 95.958 kb on + strand, within EX31_RS08025 at 95.958 kb on + strand, within EX31_RS08025 at 95.959 kb on - strand, within EX31_RS08025 at 95.959 kb on - strand, within EX31_RS08025 at 95.959 kb on - strand, within EX31_RS08025 at 95.959 kb on - strand, within EX31_RS08025 at 95.959 kb on - strand, within EX31_RS08025 at 95.959 kb on - strand, within EX31_RS08025 at 95.959 kb on - strand, within EX31_RS08025 at 95.960 kb on + strand, within EX31_RS08025 at 95.960 kb on + strand, within EX31_RS08025 at 95.960 kb on + strand, within EX31_RS08025 at 95.960 kb on + strand, within EX31_RS08025 at 95.961 kb on - strand, within EX31_RS08025 at 96.002 kb on + strand, within EX31_RS08025 at 96.003 kb on - strand, within EX31_RS08025 at 96.003 kb on - strand, within EX31_RS08025 at 96.039 kb on + strand, within EX31_RS08025 at 96.039 kb on + strand, within EX31_RS08025 at 96.039 kb on + strand, within EX31_RS08025 at 96.039 kb on + strand, within EX31_RS08025 at 96.039 kb on + strand, within EX31_RS08025 at 96.039 kb on + strand, within EX31_RS08025 at 96.039 kb on + strand, within EX31_RS08025 at 96.040 kb on - strand, within EX31_RS08025 at 96.068 kb on + strand, within EX31_RS08025 at 96.075 kb on + strand, within EX31_RS08025 at 96.075 kb on + strand, within EX31_RS08025 at 96.075 kb on + strand, within EX31_RS08025 at 96.076 kb on - strand, within EX31_RS08025 at 96.076 kb on - strand, within EX31_RS08025 at 96.076 kb on - strand, within EX31_RS08025 at 96.076 kb on - strand, within EX31_RS08025 at 96.076 kb on - strand, within EX31_RS08025 at 96.094 kb on + strand, within EX31_RS08025 at 96.095 kb on - strand, within EX31_RS08025 at 96.339 kb on + strand, within EX31_RS08025 at 96.339 kb on + strand, within EX31_RS08025 at 96.340 kb on - strand, within EX31_RS08025 at 96.440 kb on + strand at 96.440 kb on + strand at 96.440 kb on + strand at 96.440 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.445 kb on + strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.446 kb on - strand at 96.449 kb on + strand at 96.450 kb on - strand at 96.555 kb on + strand at 96.569 kb on + strand at 96.570 kb on - strand at 96.570 kb on - strand at 96.570 kb on - strand at 96.592 kb on + strand at 96.592 kb on + strand at 96.593 kb on - strand at 96.593 kb on - strand at 96.593 kb on - strand at 96.599 kb on + strand at 96.600 kb on - strand at 96.600 kb on - strand at 96.606 kb on + strand at 96.607 kb on - strand at 96.607 kb on - strand at 96.612 kb on + strand at 96.612 kb on + strand at 96.612 kb on + strand at 96.613 kb on - strand at 96.714 kb on + strand at 96.714 kb on + strand at 96.738 kb on - strand at 96.746 kb on + strand at 96.746 kb on + strand at 96.785 kb on + strand at 96.786 kb on - strand at 96.788 kb on + strand at 96.788 kb on + strand at 96.819 kb on - strand, within EX31_RS08030 at 96.822 kb on + strand, within EX31_RS08030 at 96.822 kb on + strand, within EX31_RS08030 at 96.822 kb on + strand, within EX31_RS08030 at 96.822 kb on + strand, within EX31_RS08030 at 96.823 kb on - strand, within EX31_RS08030 at 96.823 kb on - strand, within EX31_RS08030 at 96.823 kb on - strand, within EX31_RS08030 at 96.823 kb on - strand, within EX31_RS08030 at 96.823 kb on - strand, within EX31_RS08030 at 96.823 kb on - strand, within EX31_RS08030 at 96.895 kb on + strand, within EX31_RS08030 at 96.895 kb on + strand, within EX31_RS08030 at 96.895 kb on + strand, within EX31_RS08030 at 96.895 kb on + strand, within EX31_RS08030 at 96.895 kb on + strand, within EX31_RS08030 at 96.896 kb on - strand, within EX31_RS08030 at 96.896 kb on - strand, within EX31_RS08030 at 96.956 kb on + strand, within EX31_RS08030 at 96.956 kb on + strand, within EX31_RS08030 at 96.956 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.996 kb on + strand, within EX31_RS08030 at 96.997 kb on - strand, within EX31_RS08030 at 96.997 kb on - strand, within EX31_RS08030 at 96.997 kb on - strand, within EX31_RS08030 at 96.997 kb on - strand, within EX31_RS08030 at 96.997 kb on - strand, within EX31_RS08030 at 96.997 kb on - strand, within EX31_RS08030 at 96.998 kb on + strand, within EX31_RS08030 at 96.998 kb on + strand, within EX31_RS08030 at 96.998 kb on + strand, within EX31_RS08030 at 97.022 kb on + strand, within EX31_RS08030 at 97.023 kb on - strand, within EX31_RS08030 at 97.033 kb on + strand, within EX31_RS08030 at 97.034 kb on - strand, within EX31_RS08030 at 97.034 kb on - strand, within EX31_RS08030 at 97.034 kb on - strand, within EX31_RS08030 at 97.065 kb on + strand, within EX31_RS08030 at 97.065 kb on + strand, within EX31_RS08030 at 97.065 kb on + strand, within EX31_RS08030 at 97.091 kb on + strand, within EX31_RS08030 at 97.115 kb on - strand, within EX31_RS08030 at 97.280 kb on + strand, within EX31_RS08030 at 97.498 kb on + strand at 97.537 kb on + strand, within EX31_RS08035 at 97.612 kb on + strand, within EX31_RS08035 at 97.613 kb on - strand, within EX31_RS08035 at 97.624 kb on + strand, within EX31_RS08035 at 97.624 kb on + strand, within EX31_RS08035 at 97.625 kb on - strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.679 kb on + strand, within EX31_RS08035 at 97.680 kb on - strand, within EX31_RS08035 at 97.680 kb on - strand, within EX31_RS08035 at 97.715 kb on + strand, within EX31_RS08035 at 97.716 kb on - strand, within EX31_RS08035 at 97.716 kb on - strand, within EX31_RS08035 at 97.758 kb on - strand, within EX31_RS08035 at 97.758 kb on - strand, within EX31_RS08035 at 97.778 kb on + strand, within EX31_RS08035 at 97.779 kb on - strand, within EX31_RS08035 at 97.779 kb on - strand, within EX31_RS08035 at 97.779 kb on - strand, within EX31_RS08035 at 97.829 kb on + strand, within EX31_RS08035 at 97.997 kb on + strand, within EX31_RS08035 at 98.007 kb on + strand, within EX31_RS08035 at 98.007 kb on + strand, within EX31_RS08035 at 98.008 kb on - strand, within EX31_RS08035 at 98.028 kb on - strand, within EX31_RS08035 at 98.033 kb on + strand, within EX31_RS08035 at 98.116 kb on + strand, within EX31_RS08035 at 98.116 kb on + strand, within EX31_RS08035 at 98.116 kb on + strand, within EX31_RS08035 at 98.117 kb on - strand, within EX31_RS08035 at 98.117 kb on - strand, within EX31_RS08035 at 98.131 kb on + strand, within EX31_RS08035 at 98.132 kb on - strand, within EX31_RS08035 at 98.132 kb on - strand, within EX31_RS08035 at 98.200 kb on + strand, within EX31_RS08035 at 98.200 kb on + strand, within EX31_RS08035 at 98.201 kb on - strand, within EX31_RS08035 at 98.201 kb on - strand, within EX31_RS08035 at 98.201 kb on - strand, within EX31_RS08035 at 98.201 kb on - strand, within EX31_RS08035 at 98.201 kb on - strand, within EX31_RS08035 at 98.236 kb on + strand, within EX31_RS08035 at 98.236 kb on + strand, within EX31_RS08035 at 98.236 kb on + strand, within EX31_RS08035 at 98.237 kb on - strand, within EX31_RS08035 at 98.237 kb on - strand, within EX31_RS08035 at 98.237 kb on - strand, within EX31_RS08035 at 98.237 kb on - strand, within EX31_RS08035 at 98.237 kb on - strand, within EX31_RS08035 at 98.237 kb on - strand, within EX31_RS08035 at 98.280 kb on - strand at 98.365 kb on - strand at 98.387 kb on - strand at 98.394 kb on + strand at 98.394 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 95,746 + EX31_RS08025 0.35 -0.9 95,909 + EX31_RS08025 0.49 -0.3 95,933 + EX31_RS08025 0.51 -0.5 95,934 - EX31_RS08025 0.51 -1.5 95,934 - EX31_RS08025 0.51 +0.6 95,934 - EX31_RS08025 0.51 -0.5 95,958 + EX31_RS08025 0.53 +0.8 95,958 + EX31_RS08025 0.53 -0.1 95,958 + EX31_RS08025 0.53 -0.8 95,958 + EX31_RS08025 0.53 +0.5 95,958 + EX31_RS08025 0.53 +0.0 95,958 + EX31_RS08025 0.53 -0.1 95,958 + EX31_RS08025 0.53 +0.5 95,959 - EX31_RS08025 0.53 -1.7 95,959 - EX31_RS08025 0.53 -1.4 95,959 - EX31_RS08025 0.53 -0.0 95,959 - EX31_RS08025 0.53 -0.3 95,959 - EX31_RS08025 0.53 +1.0 95,959 - EX31_RS08025 0.53 -0.8 95,959 - EX31_RS08025 0.53 -0.7 95,960 + EX31_RS08025 0.53 -0.6 95,960 + EX31_RS08025 0.53 -0.2 95,960 + EX31_RS08025 0.53 +0.5 95,960 + EX31_RS08025 0.53 +0.2 95,961 - EX31_RS08025 0.53 +0.2 96,002 + EX31_RS08025 0.56 -0.0 96,003 - EX31_RS08025 0.57 -0.7 96,003 - EX31_RS08025 0.57 -0.3 96,039 + EX31_RS08025 0.60 +0.1 96,039 + EX31_RS08025 0.60 -1.3 96,039 + EX31_RS08025 0.60 -0.8 96,039 + EX31_RS08025 0.60 -0.2 96,039 + EX31_RS08025 0.60 -1.3 96,039 + EX31_RS08025 0.60 +0.8 96,039 + EX31_RS08025 0.60 -0.4 96,040 - EX31_RS08025 0.60 +1.2 96,068 + EX31_RS08025 0.62 -1.4 96,075 + EX31_RS08025 0.63 +0.1 96,075 + EX31_RS08025 0.63 -0.7 96,075 + EX31_RS08025 0.63 +0.7 96,076 - EX31_RS08025 0.63 -1.1 96,076 - EX31_RS08025 0.63 -0.1 96,076 - EX31_RS08025 0.63 -1.3 96,076 - EX31_RS08025 0.63 -0.1 96,076 - EX31_RS08025 0.63 -1.2 96,094 + EX31_RS08025 0.64 -0.4 96,095 - EX31_RS08025 0.64 -1.5 96,339 + EX31_RS08025 0.85 -0.2 96,339 + EX31_RS08025 0.85 -0.1 96,340 - EX31_RS08025 0.85 -0.4 96,440 + +0.4 96,440 + -0.4 96,440 + +0.5 96,440 + -2.3 96,445 + -0.5 96,445 + +0.1 96,445 + +0.8 96,445 + -0.6 96,445 + -1.8 96,445 + -0.3 96,445 + -0.7 96,445 + -0.3 96,445 + +0.4 96,445 + -0.2 96,445 + -0.6 96,445 + -0.6 96,445 + +0.8 96,445 + -0.6 96,445 + +0.1 96,445 + -1.5 96,445 + -0.3 96,445 + -0.1 96,445 + -1.2 96,446 - +0.6 96,446 - +0.3 96,446 - -0.9 96,446 - +0.2 96,446 - -0.4 96,446 - -0.3 96,446 - +0.1 96,446 - -0.4 96,446 - +0.0 96,446 - -0.6 96,446 - -0.5 96,446 - -0.0 96,446 - +0.2 96,446 - +0.7 96,446 - -0.0 96,446 - -0.4 96,446 - -0.6 96,446 - -1.2 96,446 - -0.4 96,446 - -1.0 96,449 + +0.2 96,450 - -1.9 96,555 + +0.3 96,569 + -0.8 96,570 - +0.7 96,570 - +0.8 96,570 - -0.8 96,592 + +1.2 96,592 + +0.5 96,593 - -0.6 96,593 - -0.7 96,593 - -1.5 96,599 + -0.5 96,600 - -1.0 96,600 - +0.8 96,606 + +0.3 96,607 - +0.0 96,607 - -0.0 96,612 + +0.8 96,612 + -0.3 96,612 + +0.3 96,613 - +0.1 96,714 + +0.3 96,714 + -1.5 96,738 - +2.0 96,746 + -0.5 96,746 + -0.5 96,785 + +0.9 96,786 - +0.6 96,788 + -0.9 96,788 + +1.7 96,819 - EX31_RS08030 0.12 +0.7 96,822 + EX31_RS08030 0.13 -1.8 96,822 + EX31_RS08030 0.13 -0.1 96,822 + EX31_RS08030 0.13 +1.4 96,822 + EX31_RS08030 0.13 +0.1 96,823 - EX31_RS08030 0.13 +0.1 96,823 - EX31_RS08030 0.13 -0.8 96,823 - EX31_RS08030 0.13 +0.3 96,823 - EX31_RS08030 0.13 +0.7 96,823 - EX31_RS08030 0.13 -0.7 96,823 - EX31_RS08030 0.13 -0.1 96,895 + EX31_RS08030 0.24 +0.6 96,895 + EX31_RS08030 0.24 +0.9 96,895 + EX31_RS08030 0.24 -0.3 96,895 + EX31_RS08030 0.24 +0.8 96,895 + EX31_RS08030 0.24 +0.6 96,896 - EX31_RS08030 0.24 -2.0 96,896 - EX31_RS08030 0.24 -2.6 96,956 + EX31_RS08030 0.33 +0.5 96,956 + EX31_RS08030 0.33 +0.8 96,956 + EX31_RS08030 0.33 +0.2 96,996 + EX31_RS08030 0.39 -0.9 96,996 + EX31_RS08030 0.39 +0.5 96,996 + EX31_RS08030 0.39 +0.0 96,996 + EX31_RS08030 0.39 +0.5 96,996 + EX31_RS08030 0.39 +0.4 96,996 + EX31_RS08030 0.39 -1.8 96,996 + EX31_RS08030 0.39 +1.4 96,996 + EX31_RS08030 0.39 +0.7 96,996 + EX31_RS08030 0.39 +0.2 96,997 - EX31_RS08030 0.39 +0.0 96,997 - EX31_RS08030 0.39 -0.3 96,997 - EX31_RS08030 0.39 +0.8 96,997 - EX31_RS08030 0.39 +0.9 96,997 - EX31_RS08030 0.39 -0.3 96,997 - EX31_RS08030 0.39 -0.3 96,998 + EX31_RS08030 0.39 +0.0 96,998 + EX31_RS08030 0.39 -0.5 96,998 + EX31_RS08030 0.39 -1.0 97,022 + EX31_RS08030 0.43 +0.8 97,023 - EX31_RS08030 0.43 -0.1 97,033 + EX31_RS08030 0.45 +0.1 97,034 - EX31_RS08030 0.45 +1.0 97,034 - EX31_RS08030 0.45 +0.4 97,034 - EX31_RS08030 0.45 -0.3 97,065 + EX31_RS08030 0.50 +1.1 97,065 + EX31_RS08030 0.50 +1.1 97,065 + EX31_RS08030 0.50 +0.1 97,091 + EX31_RS08030 0.53 -0.2 97,115 - EX31_RS08030 0.57 -1.1 97,280 + EX31_RS08030 0.82 -0.7 97,498 + -0.1 97,537 + EX31_RS08035 0.14 +0.8 97,612 + EX31_RS08035 0.22 +0.1 97,613 - EX31_RS08035 0.22 -0.3 97,624 + EX31_RS08035 0.23 +0.8 97,624 + EX31_RS08035 0.23 -0.2 97,625 - EX31_RS08035 0.23 -0.1 97,679 + EX31_RS08035 0.29 -0.8 97,679 + EX31_RS08035 0.29 -0.3 97,679 + EX31_RS08035 0.29 -0.2 97,679 + EX31_RS08035 0.29 +0.1 97,679 + EX31_RS08035 0.29 -0.1 97,679 + EX31_RS08035 0.29 -0.0 97,679 + EX31_RS08035 0.29 +0.9 97,679 + EX31_RS08035 0.29 +0.5 97,679 + EX31_RS08035 0.29 +0.5 97,679 + EX31_RS08035 0.29 +1.0 97,680 - EX31_RS08035 0.29 +1.1 97,680 - EX31_RS08035 0.29 +2.2 97,715 + EX31_RS08035 0.33 +0.1 97,716 - EX31_RS08035 0.33 +0.8 97,716 - EX31_RS08035 0.33 -0.4 97,758 - EX31_RS08035 0.38 -0.8 97,758 - EX31_RS08035 0.38 -0.0 97,778 + EX31_RS08035 0.40 +0.3 97,779 - EX31_RS08035 0.40 +0.4 97,779 - EX31_RS08035 0.40 +1.0 97,779 - EX31_RS08035 0.40 +1.0 97,829 + EX31_RS08035 0.45 +0.5 97,997 + EX31_RS08035 0.63 -1.4 98,007 + EX31_RS08035 0.64 +0.4 98,007 + EX31_RS08035 0.64 -0.8 98,008 - EX31_RS08035 0.64 +0.0 98,028 - EX31_RS08035 0.66 -0.0 98,033 + EX31_RS08035 0.67 -0.0 98,116 + EX31_RS08035 0.76 +0.7 98,116 + EX31_RS08035 0.76 +0.4 98,116 + EX31_RS08035 0.76 -0.5 98,117 - EX31_RS08035 0.76 +0.2 98,117 - EX31_RS08035 0.76 -0.8 98,131 + EX31_RS08035 0.77 -1.8 98,132 - EX31_RS08035 0.77 +0.5 98,132 - EX31_RS08035 0.77 +0.8 98,200 + EX31_RS08035 0.85 +1.0 98,200 + EX31_RS08035 0.85 +0.0 98,201 - EX31_RS08035 0.85 -1.8 98,201 - EX31_RS08035 0.85 -0.8 98,201 - EX31_RS08035 0.85 +1.0 98,201 - EX31_RS08035 0.85 -0.3 98,201 - EX31_RS08035 0.85 -0.6 98,236 + EX31_RS08035 0.89 -0.2 98,236 + EX31_RS08035 0.89 -0.1 98,236 + EX31_RS08035 0.89 -0.2 98,237 - EX31_RS08035 0.89 -0.3 98,237 - EX31_RS08035 0.89 -0.8 98,237 - EX31_RS08035 0.89 -0.1 98,237 - EX31_RS08035 0.89 -0.9 98,237 - EX31_RS08035 0.89 +0.1 98,237 - EX31_RS08035 0.89 +0.0 98,280 - -0.2 98,365 - +0.5 98,387 - -0.0 98,394 + +0.1 98,394 + +0.4
Or see this region's nucleotide sequence