Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07155 and EX31_RS07160 are separated by 138 nucleotides EX31_RS07160 and EX31_RS07165 are separated by 192 nucleotides
EX31_RS07155: EX31_RS07155 - exoribonuclease II, at 225,272 to 227,215
_RS07155
EX31_RS07160: EX31_RS07160 - HTH-type transcriptional regulator Cbl, at 227,354 to 228,307
_RS07160
EX31_RS07165: EX31_RS07165 - 2-hydroxyacid dehydrogenase, at 228,500 to 229,444
_RS07165
Position (kb) 227
228
229 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 226.354 kb on + strand, within EX31_RS07155 at 226.355 kb on - strand, within EX31_RS07155 at 226.517 kb on + strand, within EX31_RS07155 at 226.517 kb on + strand, within EX31_RS07155 at 226.754 kb on + strand, within EX31_RS07155 at 226.774 kb on + strand, within EX31_RS07155 at 226.780 kb on + strand, within EX31_RS07155 at 226.928 kb on + strand, within EX31_RS07155 at 226.928 kb on + strand, within EX31_RS07155 at 226.929 kb on - strand, within EX31_RS07155 at 226.949 kb on - strand, within EX31_RS07155 at 226.966 kb on + strand, within EX31_RS07155 at 227.013 kb on + strand, within EX31_RS07155 at 227.013 kb on + strand, within EX31_RS07155 at 227.013 kb on + strand, within EX31_RS07155 at 227.014 kb on - strand, within EX31_RS07155 at 227.014 kb on - strand, within EX31_RS07155 at 227.014 kb on - strand, within EX31_RS07155 at 227.116 kb on + strand at 227.116 kb on + strand at 227.117 kb on - strand at 227.213 kb on + strand at 227.397 kb on - strand at 227.456 kb on + strand, within EX31_RS07160 at 227.461 kb on - strand, within EX31_RS07160 at 227.461 kb on - strand, within EX31_RS07160 at 227.461 kb on - strand, within EX31_RS07160 at 227.504 kb on - strand, within EX31_RS07160 at 227.516 kb on + strand, within EX31_RS07160 at 227.618 kb on - strand, within EX31_RS07160 at 227.618 kb on - strand, within EX31_RS07160 at 227.718 kb on + strand, within EX31_RS07160 at 227.718 kb on + strand, within EX31_RS07160 at 227.719 kb on - strand, within EX31_RS07160 at 227.719 kb on - strand, within EX31_RS07160 at 227.820 kb on + strand, within EX31_RS07160 at 227.953 kb on - strand, within EX31_RS07160 at 228.165 kb on - strand, within EX31_RS07160 at 228.243 kb on - strand at 228.339 kb on + strand at 228.386 kb on - strand at 228.389 kb on + strand at 228.574 kb on + strand at 228.575 kb on - strand at 228.760 kb on + strand, within EX31_RS07165 at 228.796 kb on + strand, within EX31_RS07165 at 228.944 kb on - strand, within EX31_RS07165 at 229.033 kb on + strand, within EX31_RS07165 at 229.067 kb on + strand, within EX31_RS07165 at 229.067 kb on + strand, within EX31_RS07165 at 229.068 kb on - strand, within EX31_RS07165 at 229.123 kb on + strand, within EX31_RS07165 at 229.124 kb on - strand, within EX31_RS07165 at 229.124 kb on - strand, within EX31_RS07165 at 229.144 kb on + strand, within EX31_RS07165 at 229.145 kb on - strand, within EX31_RS07165 at 229.174 kb on + strand, within EX31_RS07165 at 229.204 kb on + strand, within EX31_RS07165 at 229.205 kb on - strand, within EX31_RS07165 at 229.205 kb on - strand, within EX31_RS07165 at 229.205 kb on - strand, within EX31_RS07165 at 229.275 kb on - strand, within EX31_RS07165 at 229.295 kb on + strand, within EX31_RS07165
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 226,354 + EX31_RS07155 0.56 +0.5 226,355 - EX31_RS07155 0.56 +1.8 226,517 + EX31_RS07155 0.64 +1.2 226,517 + EX31_RS07155 0.64 +0.2 226,754 + EX31_RS07155 0.76 -0.4 226,774 + EX31_RS07155 0.77 +1.0 226,780 + EX31_RS07155 0.78 +1.5 226,928 + EX31_RS07155 0.85 +0.3 226,928 + EX31_RS07155 0.85 +1.8 226,929 - EX31_RS07155 0.85 +1.8 226,949 - EX31_RS07155 0.86 -0.5 226,966 + EX31_RS07155 0.87 +0.6 227,013 + EX31_RS07155 0.90 +1.0 227,013 + EX31_RS07155 0.90 -0.4 227,013 + EX31_RS07155 0.90 +2.2 227,014 - EX31_RS07155 0.90 +1.1 227,014 - EX31_RS07155 0.90 -0.5 227,014 - EX31_RS07155 0.90 +0.8 227,116 + +1.8 227,116 + +0.8 227,117 - +2.6 227,213 + +0.2 227,397 - +1.3 227,456 + EX31_RS07160 0.11 -0.2 227,461 - EX31_RS07160 0.11 +1.6 227,461 - EX31_RS07160 0.11 -0.7 227,461 - EX31_RS07160 0.11 -0.4 227,504 - EX31_RS07160 0.16 +0.7 227,516 + EX31_RS07160 0.17 +1.2 227,618 - EX31_RS07160 0.28 +0.2 227,618 - EX31_RS07160 0.28 -0.0 227,718 + EX31_RS07160 0.38 +2.8 227,718 + EX31_RS07160 0.38 -0.3 227,719 - EX31_RS07160 0.38 -1.4 227,719 - EX31_RS07160 0.38 -0.3 227,820 + EX31_RS07160 0.49 -0.1 227,953 - EX31_RS07160 0.63 +0.5 228,165 - EX31_RS07160 0.85 -0.8 228,243 - +0.7 228,339 + -1.0 228,386 - +1.5 228,389 + +1.8 228,574 + +1.2 228,575 - -1.5 228,760 + EX31_RS07165 0.28 -1.2 228,796 + EX31_RS07165 0.31 -0.5 228,944 - EX31_RS07165 0.47 +1.3 229,033 + EX31_RS07165 0.56 +2.5 229,067 + EX31_RS07165 0.60 +1.5 229,067 + EX31_RS07165 0.60 -2.6 229,068 - EX31_RS07165 0.60 +1.0 229,123 + EX31_RS07165 0.66 -2.5 229,124 - EX31_RS07165 0.66 +0.8 229,124 - EX31_RS07165 0.66 -2.2 229,144 + EX31_RS07165 0.68 +0.1 229,145 - EX31_RS07165 0.68 -0.2 229,174 + EX31_RS07165 0.71 -0.4 229,204 + EX31_RS07165 0.74 +0.2 229,205 - EX31_RS07165 0.75 -0.8 229,205 - EX31_RS07165 0.75 +0.7 229,205 - EX31_RS07165 0.75 -0.5 229,275 - EX31_RS07165 0.82 -1.0 229,295 + EX31_RS07165 0.84 -0.0
Or see this region's nucleotide sequence