Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS03770 and EX31_RS03775 overlap by 11 nucleotides EX31_RS03775 and EX31_RS03780 are separated by 5 nucleotides EX31_RS03780 and EX31_RS03785 are separated by 4 nucleotides
EX31_RS03770: EX31_RS03770 - peptidylprolyl isomerase SurA, at 262,867 to 264,162
_RS03770
EX31_RS03775: EX31_RS03775 - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 264,152 to 265,159
_RS03775
EX31_RS03780: EX31_RS03780 - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA, at 265,165 to 265,992
_RS03780
EX31_RS03785: EX31_RS03785 - Co2+/Mg2+ efflux protein ApaG, at 265,997 to 266,374
_RS03785
Position (kb) 264
265
266 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 263.157 kb on + strand, within EX31_RS03770 at 263.224 kb on + strand, within EX31_RS03770 at 263.224 kb on + strand, within EX31_RS03770 at 263.239 kb on + strand, within EX31_RS03770 at 263.239 kb on + strand, within EX31_RS03770 at 263.239 kb on + strand, within EX31_RS03770 at 263.239 kb on + strand, within EX31_RS03770 at 263.239 kb on + strand, within EX31_RS03770 at 263.248 kb on + strand, within EX31_RS03770 at 263.248 kb on + strand, within EX31_RS03770 at 263.248 kb on + strand, within EX31_RS03770 at 263.288 kb on + strand, within EX31_RS03770 at 263.288 kb on + strand, within EX31_RS03770 at 263.288 kb on + strand, within EX31_RS03770 at 263.292 kb on + strand, within EX31_RS03770 at 263.292 kb on + strand, within EX31_RS03770 at 263.292 kb on + strand, within EX31_RS03770 at 263.292 kb on + strand, within EX31_RS03770 at 263.292 kb on + strand, within EX31_RS03770 at 263.321 kb on + strand, within EX31_RS03770 at 263.521 kb on + strand, within EX31_RS03770 at 263.521 kb on + strand, within EX31_RS03770 at 263.521 kb on + strand, within EX31_RS03770 at 263.521 kb on + strand, within EX31_RS03770 at 263.697 kb on + strand, within EX31_RS03770 at 263.756 kb on + strand, within EX31_RS03770 at 263.808 kb on + strand, within EX31_RS03770 at 263.808 kb on + strand, within EX31_RS03770 at 263.808 kb on + strand, within EX31_RS03770 at 263.808 kb on + strand, within EX31_RS03770 at 263.808 kb on + strand, within EX31_RS03770 at 263.871 kb on + strand, within EX31_RS03770 at 263.888 kb on + strand, within EX31_RS03770 at 263.907 kb on + strand, within EX31_RS03770 at 263.907 kb on + strand, within EX31_RS03770 at 263.907 kb on + strand, within EX31_RS03770 at 263.907 kb on + strand, within EX31_RS03770 at 263.907 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 263.911 kb on + strand, within EX31_RS03770 at 264.006 kb on + strand, within EX31_RS03770 at 264.006 kb on + strand, within EX31_RS03770 at 264.027 kb on + strand, within EX31_RS03770 at 264.058 kb on + strand at 264.058 kb on + strand at 264.063 kb on + strand at 264.130 kb on + strand at 264.130 kb on + strand at 264.130 kb on + strand at 264.130 kb on + strand at 264.141 kb on + strand at 264.163 kb on + strand at 264.163 kb on + strand at 264.264 kb on + strand, within EX31_RS03775 at 264.264 kb on + strand, within EX31_RS03775 at 264.691 kb on + strand, within EX31_RS03775 at 264.757 kb on + strand, within EX31_RS03775 at 264.773 kb on + strand, within EX31_RS03775 at 264.838 kb on + strand, within EX31_RS03775 at 264.956 kb on + strand, within EX31_RS03775 at 264.956 kb on + strand, within EX31_RS03775 at 264.981 kb on + strand, within EX31_RS03775 at 264.981 kb on + strand, within EX31_RS03775 at 264.986 kb on + strand, within EX31_RS03775 at 265.012 kb on + strand, within EX31_RS03775 at 265.141 kb on + strand at 265.990 kb on + strand at 265.996 kb on + strand at 265.997 kb on - strand at 266.002 kb on + strand at 266.002 kb on + strand at 266.002 kb on + strand at 266.002 kb on + strand at 266.002 kb on + strand at 266.003 kb on - strand at 266.003 kb on - strand at 266.003 kb on - strand at 266.035 kb on + strand, within EX31_RS03785 at 266.035 kb on + strand, within EX31_RS03785 at 266.035 kb on + strand, within EX31_RS03785 at 266.035 kb on + strand, within EX31_RS03785 at 266.035 kb on + strand, within EX31_RS03785 at 266.035 kb on + strand, within EX31_RS03785 at 266.036 kb on - strand, within EX31_RS03785 at 266.039 kb on + strand, within EX31_RS03785 at 266.039 kb on + strand, within EX31_RS03785 at 266.039 kb on + strand, within EX31_RS03785 at 266.039 kb on + strand, within EX31_RS03785 at 266.039 kb on + strand, within EX31_RS03785 at 266.039 kb on + strand, within EX31_RS03785 at 266.040 kb on - strand, within EX31_RS03785 at 266.040 kb on - strand, within EX31_RS03785 at 266.087 kb on + strand, within EX31_RS03785 at 266.087 kb on + strand, within EX31_RS03785 at 266.087 kb on + strand, within EX31_RS03785 at 266.119 kb on + strand, within EX31_RS03785 at 266.119 kb on + strand, within EX31_RS03785 at 266.120 kb on - strand, within EX31_RS03785 at 266.151 kb on + strand, within EX31_RS03785
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 263,157 + EX31_RS03770 0.22 -0.1 263,224 + EX31_RS03770 0.28 +1.9 263,224 + EX31_RS03770 0.28 +0.2 263,239 + EX31_RS03770 0.29 -0.7 263,239 + EX31_RS03770 0.29 +0.6 263,239 + EX31_RS03770 0.29 -0.2 263,239 + EX31_RS03770 0.29 +0.2 263,239 + EX31_RS03770 0.29 -0.8 263,248 + EX31_RS03770 0.29 -0.7 263,248 + EX31_RS03770 0.29 +1.1 263,248 + EX31_RS03770 0.29 +0.8 263,288 + EX31_RS03770 0.32 -0.3 263,288 + EX31_RS03770 0.32 +0.5 263,288 + EX31_RS03770 0.32 +0.1 263,292 + EX31_RS03770 0.33 -0.4 263,292 + EX31_RS03770 0.33 -0.3 263,292 + EX31_RS03770 0.33 -0.3 263,292 + EX31_RS03770 0.33 -0.1 263,292 + EX31_RS03770 0.33 +0.5 263,321 + EX31_RS03770 0.35 +1.4 263,521 + EX31_RS03770 0.50 +0.4 263,521 + EX31_RS03770 0.50 +0.3 263,521 + EX31_RS03770 0.50 -0.1 263,521 + EX31_RS03770 0.50 -0.1 263,697 + EX31_RS03770 0.64 -0.4 263,756 + EX31_RS03770 0.69 +0.5 263,808 + EX31_RS03770 0.73 +1.1 263,808 + EX31_RS03770 0.73 +0.4 263,808 + EX31_RS03770 0.73 -0.7 263,808 + EX31_RS03770 0.73 +1.5 263,808 + EX31_RS03770 0.73 -0.8 263,871 + EX31_RS03770 0.77 -0.8 263,888 + EX31_RS03770 0.79 +0.5 263,907 + EX31_RS03770 0.80 -0.4 263,907 + EX31_RS03770 0.80 +0.3 263,907 + EX31_RS03770 0.80 -0.4 263,907 + EX31_RS03770 0.80 -0.3 263,907 + EX31_RS03770 0.80 -1.2 263,911 + EX31_RS03770 0.81 -0.6 263,911 + EX31_RS03770 0.81 -0.6 263,911 + EX31_RS03770 0.81 -0.2 263,911 + EX31_RS03770 0.81 -0.1 263,911 + EX31_RS03770 0.81 -1.7 263,911 + EX31_RS03770 0.81 -1.9 263,911 + EX31_RS03770 0.81 -0.0 263,911 + EX31_RS03770 0.81 -1.8 264,006 + EX31_RS03770 0.88 -3.0 264,006 + EX31_RS03770 0.88 -0.9 264,027 + EX31_RS03770 0.90 +0.7 264,058 + -0.6 264,058 + -0.0 264,063 + +0.2 264,130 + -0.2 264,130 + +0.1 264,130 + +0.7 264,130 + +0.2 264,141 + -0.4 264,163 + -3.0 264,163 + -1.7 264,264 + EX31_RS03775 0.11 -2.6 264,264 + EX31_RS03775 0.11 -2.2 264,691 + EX31_RS03775 0.53 -0.5 264,757 + EX31_RS03775 0.60 -2.8 264,773 + EX31_RS03775 0.62 -2.4 264,838 + EX31_RS03775 0.68 -2.0 264,956 + EX31_RS03775 0.80 -2.4 264,956 + EX31_RS03775 0.80 -1.7 264,981 + EX31_RS03775 0.82 -1.9 264,981 + EX31_RS03775 0.82 -1.8 264,986 + EX31_RS03775 0.83 -1.9 265,012 + EX31_RS03775 0.85 -0.2 265,141 + -1.4 265,990 + +2.1 265,996 + -0.7 265,997 - -0.2 266,002 + +1.1 266,002 + -1.1 266,002 + +0.5 266,002 + -0.2 266,002 + +0.7 266,003 - -0.8 266,003 - -0.4 266,003 - -2.1 266,035 + EX31_RS03785 0.10 +0.1 266,035 + EX31_RS03785 0.10 +0.3 266,035 + EX31_RS03785 0.10 -0.1 266,035 + EX31_RS03785 0.10 -0.1 266,035 + EX31_RS03785 0.10 +1.2 266,035 + EX31_RS03785 0.10 +0.0 266,036 - EX31_RS03785 0.10 -0.8 266,039 + EX31_RS03785 0.11 +0.3 266,039 + EX31_RS03785 0.11 -0.5 266,039 + EX31_RS03785 0.11 +1.4 266,039 + EX31_RS03785 0.11 +0.4 266,039 + EX31_RS03785 0.11 -0.8 266,039 + EX31_RS03785 0.11 +0.1 266,040 - EX31_RS03785 0.11 +0.7 266,040 - EX31_RS03785 0.11 +0.0 266,087 + EX31_RS03785 0.24 +0.3 266,087 + EX31_RS03785 0.24 +0.1 266,087 + EX31_RS03785 0.24 -0.5 266,119 + EX31_RS03785 0.32 +0.8 266,119 + EX31_RS03785 0.32 -0.4 266,120 - EX31_RS03785 0.33 -1.1 266,151 + EX31_RS03785 0.41 +0.1
Or see this region's nucleotide sequence