Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02060 and EX31_RS02065 are separated by 60 nucleotides EX31_RS02065 and EX31_RS02070 are separated by 533 nucleotides
EX31_RS02060: EX31_RS02060 - AraC family transcriptional regulator, at 451,117 to 452,103
_RS02060
EX31_RS02065: EX31_RS02065 - MFS transporter, at 452,164 to 453,402
_RS02065
EX31_RS02070: EX31_RS02070 - cytochrome ubiquinol oxidase subunit I, at 453,936 to 455,369
_RS02070
Position (kb) 452
453
454 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 451.186 kb on - strand at 451.186 kb on - strand at 451.186 kb on - strand at 451.187 kb on + strand at 451.187 kb on + strand at 451.550 kb on - strand, within EX31_RS02060 at 451.550 kb on - strand, within EX31_RS02060 at 451.550 kb on - strand, within EX31_RS02060 at 451.593 kb on - strand, within EX31_RS02060 at 451.683 kb on - strand, within EX31_RS02060 at 451.683 kb on - strand, within EX31_RS02060 at 451.725 kb on + strand, within EX31_RS02060 at 451.726 kb on - strand, within EX31_RS02060 at 451.751 kb on - strand, within EX31_RS02060 at 451.885 kb on - strand, within EX31_RS02060 at 451.885 kb on - strand, within EX31_RS02060 at 451.885 kb on - strand, within EX31_RS02060 at 451.910 kb on + strand, within EX31_RS02060 at 451.911 kb on - strand, within EX31_RS02060 at 451.911 kb on - strand, within EX31_RS02060 at 451.911 kb on - strand, within EX31_RS02060 at 451.919 kb on + strand, within EX31_RS02060 at 451.919 kb on + strand, within EX31_RS02060 at 451.920 kb on - strand, within EX31_RS02060 at 452.126 kb on + strand at 452.138 kb on + strand at 452.138 kb on + strand at 452.139 kb on - strand at 452.139 kb on - strand at 452.139 kb on - strand at 452.172 kb on + strand at 452.172 kb on + strand at 452.284 kb on + strand at 452.449 kb on - strand, within EX31_RS02065 at 452.620 kb on - strand, within EX31_RS02065 at 452.631 kb on + strand, within EX31_RS02065 at 452.632 kb on - strand, within EX31_RS02065 at 452.686 kb on + strand, within EX31_RS02065 at 452.785 kb on - strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.790 kb on + strand, within EX31_RS02065 at 452.791 kb on - strand, within EX31_RS02065 at 452.791 kb on - strand, within EX31_RS02065 at 452.791 kb on - strand, within EX31_RS02065 at 452.791 kb on - strand, within EX31_RS02065 at 452.791 kb on - strand, within EX31_RS02065 at 452.791 kb on - strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.792 kb on + strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.793 kb on - strand, within EX31_RS02065 at 452.804 kb on + strand, within EX31_RS02065 at 452.862 kb on + strand, within EX31_RS02065 at 452.862 kb on + strand, within EX31_RS02065 at 452.872 kb on + strand, within EX31_RS02065 at 452.873 kb on - strand, within EX31_RS02065 at 452.873 kb on - strand, within EX31_RS02065 at 452.873 kb on - strand, within EX31_RS02065 at 452.883 kb on + strand, within EX31_RS02065 at 452.884 kb on - strand, within EX31_RS02065 at 452.884 kb on - strand, within EX31_RS02065 at 452.887 kb on + strand, within EX31_RS02065 at 452.888 kb on - strand, within EX31_RS02065 at 452.888 kb on - strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.921 kb on + strand, within EX31_RS02065 at 452.922 kb on - strand, within EX31_RS02065 at 452.922 kb on - strand, within EX31_RS02065 at 452.922 kb on - strand, within EX31_RS02065 at 452.922 kb on - strand, within EX31_RS02065 at 453.008 kb on - strand, within EX31_RS02065 at 453.032 kb on + strand, within EX31_RS02065 at 453.071 kb on + strand, within EX31_RS02065 at 453.072 kb on - strand, within EX31_RS02065 at 453.075 kb on - strand, within EX31_RS02065 at 453.075 kb on - strand, within EX31_RS02065 at 453.140 kb on + strand, within EX31_RS02065 at 453.140 kb on + strand, within EX31_RS02065 at 453.140 kb on + strand, within EX31_RS02065 at 453.294 kb on + strand at 453.317 kb on + strand at 453.317 kb on + strand at 453.318 kb on - strand at 453.578 kb on - strand at 453.713 kb on + strand at 453.714 kb on - strand at 453.863 kb on + strand at 453.865 kb on + strand at 453.866 kb on - strand at 453.878 kb on + strand at 453.879 kb on - strand at 453.879 kb on - strand at 453.879 kb on - strand at 453.881 kb on + strand at 453.881 kb on + strand at 453.881 kb on + strand at 453.881 kb on + strand at 453.881 kb on + strand at 453.881 kb on + strand at 453.882 kb on - strand at 453.882 kb on - strand at 453.882 kb on - strand at 453.882 kb on - strand at 453.882 kb on - strand at 453.882 kb on - strand at 453.882 kb on - strand at 453.882 kb on - strand at 453.885 kb on + strand at 453.885 kb on + strand at 453.886 kb on - strand at 453.886 kb on - strand at 453.886 kb on - strand at 453.890 kb on + strand at 453.896 kb on + strand at 453.946 kb on + strand at 453.947 kb on - strand at 453.970 kb on + strand at 453.970 kb on + strand at 453.970 kb on + strand at 453.970 kb on + strand at 454.003 kb on + strand at 454.003 kb on + strand at 454.004 kb on - strand at 454.125 kb on - strand, within EX31_RS02070 at 454.233 kb on + strand, within EX31_RS02070 at 454.233 kb on + strand, within EX31_RS02070 at 454.233 kb on + strand, within EX31_RS02070 at 454.233 kb on + strand, within EX31_RS02070 at 454.234 kb on - strand, within EX31_RS02070 at 454.234 kb on - strand, within EX31_RS02070 at 454.234 kb on - strand, within EX31_RS02070 at 454.234 kb on - strand, within EX31_RS02070 at 454.335 kb on - strand, within EX31_RS02070 at 454.355 kb on + strand, within EX31_RS02070 at 454.355 kb on + strand, within EX31_RS02070 at 454.356 kb on - strand, within EX31_RS02070 at 454.356 kb on - strand, within EX31_RS02070 at 454.397 kb on + strand, within EX31_RS02070
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 451,186 - -0.4 451,186 - +0.8 451,186 - -1.1 451,187 + -0.1 451,187 + -0.1 451,550 - EX31_RS02060 0.44 +0.9 451,550 - EX31_RS02060 0.44 +1.2 451,550 - EX31_RS02060 0.44 +0.2 451,593 - EX31_RS02060 0.48 +0.3 451,683 - EX31_RS02060 0.57 -1.5 451,683 - EX31_RS02060 0.57 -0.3 451,725 + EX31_RS02060 0.62 -0.1 451,726 - EX31_RS02060 0.62 +0.3 451,751 - EX31_RS02060 0.64 -0.3 451,885 - EX31_RS02060 0.78 -0.1 451,885 - EX31_RS02060 0.78 +1.5 451,885 - EX31_RS02060 0.78 +0.5 451,910 + EX31_RS02060 0.80 -0.6 451,911 - EX31_RS02060 0.80 +0.4 451,911 - EX31_RS02060 0.80 +1.5 451,911 - EX31_RS02060 0.80 -1.3 451,919 + EX31_RS02060 0.81 +0.8 451,919 + EX31_RS02060 0.81 +1.4 451,920 - EX31_RS02060 0.81 -1.7 452,126 + -1.1 452,138 + +1.8 452,138 + +2.5 452,139 - +0.6 452,139 - +1.5 452,139 - -1.7 452,172 + +0.4 452,172 + +0.5 452,284 + -0.1 452,449 - EX31_RS02065 0.23 -0.8 452,620 - EX31_RS02065 0.37 -1.6 452,631 + EX31_RS02065 0.38 +1.5 452,632 - EX31_RS02065 0.38 +0.9 452,686 + EX31_RS02065 0.42 +1.1 452,785 - EX31_RS02065 0.50 -0.4 452,790 + EX31_RS02065 0.51 -0.1 452,790 + EX31_RS02065 0.51 +1.6 452,790 + EX31_RS02065 0.51 +0.8 452,790 + EX31_RS02065 0.51 +0.6 452,790 + EX31_RS02065 0.51 +0.9 452,790 + EX31_RS02065 0.51 +0.3 452,790 + EX31_RS02065 0.51 +0.2 452,790 + EX31_RS02065 0.51 -1.8 452,790 + EX31_RS02065 0.51 +0.7 452,791 - EX31_RS02065 0.51 +1.2 452,791 - EX31_RS02065 0.51 -0.7 452,791 - EX31_RS02065 0.51 -1.8 452,791 - EX31_RS02065 0.51 +0.8 452,791 - EX31_RS02065 0.51 -0.0 452,791 - EX31_RS02065 0.51 +0.7 452,792 + EX31_RS02065 0.51 +0.3 452,792 + EX31_RS02065 0.51 +0.3 452,792 + EX31_RS02065 0.51 +1.3 452,792 + EX31_RS02065 0.51 +0.6 452,792 + EX31_RS02065 0.51 -0.5 452,792 + EX31_RS02065 0.51 -0.5 452,792 + EX31_RS02065 0.51 -0.7 452,792 + EX31_RS02065 0.51 +1.3 452,792 + EX31_RS02065 0.51 -1.4 452,793 - EX31_RS02065 0.51 -1.0 452,793 - EX31_RS02065 0.51 -1.6 452,793 - EX31_RS02065 0.51 +1.0 452,793 - EX31_RS02065 0.51 +0.4 452,793 - EX31_RS02065 0.51 +0.4 452,793 - EX31_RS02065 0.51 -0.8 452,793 - EX31_RS02065 0.51 -0.9 452,793 - EX31_RS02065 0.51 -0.3 452,793 - EX31_RS02065 0.51 -0.7 452,793 - EX31_RS02065 0.51 +1.5 452,793 - EX31_RS02065 0.51 -0.3 452,804 + EX31_RS02065 0.52 -0.2 452,862 + EX31_RS02065 0.56 +3.5 452,862 + EX31_RS02065 0.56 -0.4 452,872 + EX31_RS02065 0.57 +2.2 452,873 - EX31_RS02065 0.57 +0.9 452,873 - EX31_RS02065 0.57 +1.2 452,873 - EX31_RS02065 0.57 -1.4 452,883 + EX31_RS02065 0.58 +1.2 452,884 - EX31_RS02065 0.58 +1.0 452,884 - EX31_RS02065 0.58 -1.2 452,887 + EX31_RS02065 0.58 -0.8 452,888 - EX31_RS02065 0.58 +0.6 452,888 - EX31_RS02065 0.58 +0.9 452,921 + EX31_RS02065 0.61 -0.9 452,921 + EX31_RS02065 0.61 +1.6 452,921 + EX31_RS02065 0.61 +0.6 452,921 + EX31_RS02065 0.61 +0.7 452,921 + EX31_RS02065 0.61 +1.0 452,921 + EX31_RS02065 0.61 +2.2 452,921 + EX31_RS02065 0.61 +0.6 452,921 + EX31_RS02065 0.61 +1.1 452,921 + EX31_RS02065 0.61 +0.2 452,921 + EX31_RS02065 0.61 +0.4 452,921 + EX31_RS02065 0.61 +1.5 452,922 - EX31_RS02065 0.61 -1.4 452,922 - EX31_RS02065 0.61 -0.3 452,922 - EX31_RS02065 0.61 -0.5 452,922 - EX31_RS02065 0.61 +1.1 453,008 - EX31_RS02065 0.68 -1.2 453,032 + EX31_RS02065 0.70 +0.4 453,071 + EX31_RS02065 0.73 +0.0 453,072 - EX31_RS02065 0.73 +0.1 453,075 - EX31_RS02065 0.74 +1.8 453,075 - EX31_RS02065 0.74 +1.2 453,140 + EX31_RS02065 0.79 -0.5 453,140 + EX31_RS02065 0.79 -0.2 453,140 + EX31_RS02065 0.79 -0.9 453,294 + -2.1 453,317 + -1.3 453,317 + -0.1 453,318 - -0.1 453,578 - +0.5 453,713 + +0.9 453,714 - +1.0 453,863 + -0.1 453,865 + +0.2 453,866 - -0.1 453,878 + +1.0 453,879 - +0.1 453,879 - +1.2 453,879 - -0.2 453,881 + -0.7 453,881 + +1.3 453,881 + -0.8 453,881 + +0.4 453,881 + -0.5 453,881 + -0.0 453,882 - +0.3 453,882 - +1.1 453,882 - +1.7 453,882 - -1.3 453,882 - -0.4 453,882 - -0.9 453,882 - +0.6 453,882 - -0.6 453,885 + +0.6 453,885 + -0.1 453,886 - +1.2 453,886 - +0.2 453,886 - +0.8 453,890 + +0.2 453,896 + -0.1 453,946 + -0.6 453,947 - -0.4 453,970 + -2.3 453,970 + -0.9 453,970 + +0.3 453,970 + +1.2 454,003 + +1.4 454,003 + +1.1 454,004 - -0.1 454,125 - EX31_RS02070 0.13 +1.0 454,233 + EX31_RS02070 0.21 +0.2 454,233 + EX31_RS02070 0.21 -1.5 454,233 + EX31_RS02070 0.21 +1.2 454,233 + EX31_RS02070 0.21 -0.5 454,234 - EX31_RS02070 0.21 -0.6 454,234 - EX31_RS02070 0.21 -0.1 454,234 - EX31_RS02070 0.21 -0.7 454,234 - EX31_RS02070 0.21 +1.8 454,335 - EX31_RS02070 0.28 +0.9 454,355 + EX31_RS02070 0.29 +1.8 454,355 + EX31_RS02070 0.29 -0.7 454,356 - EX31_RS02070 0.29 -0.4 454,356 - EX31_RS02070 0.29 +0.5 454,397 + EX31_RS02070 0.32 -0.1
Or see this region's nucleotide sequence