Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01110 and EX31_RS01115 are separated by 70 nucleotides EX31_RS01115 and EX31_RS01120 are separated by 351 nucleotides
EX31_RS01110: EX31_RS01110 - hypothetical protein, at 241,436 to 242,170
_RS01110
EX31_RS01115: EX31_RS01115 - ABC-F family ATP-binding cassette domain-containing protein, at 242,241 to 243,974
_RS01115
EX31_RS01120: EX31_RS01120 - MFS transporter, at 244,326 to 245,564
_RS01120
Position (kb) 242
243
244 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 241.266 kb on + strand at 241.312 kb on + strand at 241.312 kb on + strand at 241.333 kb on + strand at 241.333 kb on + strand at 241.359 kb on + strand at 241.374 kb on + strand at 241.382 kb on + strand at 241.438 kb on - strand at 241.611 kb on + strand, within EX31_RS01110 at 241.611 kb on + strand, within EX31_RS01110 at 241.611 kb on + strand, within EX31_RS01110 at 241.612 kb on - strand, within EX31_RS01110 at 241.612 kb on - strand, within EX31_RS01110 at 241.657 kb on + strand, within EX31_RS01110 at 241.681 kb on - strand, within EX31_RS01110 at 241.686 kb on + strand, within EX31_RS01110 at 241.686 kb on + strand, within EX31_RS01110 at 241.687 kb on - strand, within EX31_RS01110 at 241.687 kb on - strand, within EX31_RS01110 at 241.688 kb on + strand, within EX31_RS01110 at 241.689 kb on - strand, within EX31_RS01110 at 241.689 kb on - strand, within EX31_RS01110 at 241.689 kb on - strand, within EX31_RS01110 at 241.969 kb on + strand, within EX31_RS01110 at 241.969 kb on + strand, within EX31_RS01110 at 241.969 kb on + strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.970 kb on - strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.971 kb on + strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.972 kb on - strand, within EX31_RS01110 at 241.993 kb on + strand, within EX31_RS01110 at 241.993 kb on + strand, within EX31_RS01110 at 242.203 kb on + strand at 242.203 kb on + strand at 242.208 kb on - strand at 242.218 kb on - strand at 242.220 kb on - strand at 242.230 kb on - strand at 242.234 kb on + strand at 242.234 kb on + strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.235 kb on - strand at 242.242 kb on + strand at 242.243 kb on - strand at 242.243 kb on - strand at 242.474 kb on - strand, within EX31_RS01115 at 242.474 kb on - strand, within EX31_RS01115 at 242.658 kb on + strand, within EX31_RS01115 at 242.671 kb on + strand, within EX31_RS01115 at 242.672 kb on - strand, within EX31_RS01115 at 242.755 kb on + strand, within EX31_RS01115 at 242.872 kb on + strand, within EX31_RS01115 at 242.876 kb on - strand, within EX31_RS01115 at 242.933 kb on + strand, within EX31_RS01115 at 243.492 kb on + strand, within EX31_RS01115 at 243.493 kb on - strand, within EX31_RS01115 at 243.537 kb on + strand, within EX31_RS01115 at 243.725 kb on + strand, within EX31_RS01115 at 243.725 kb on + strand, within EX31_RS01115 at 243.726 kb on - strand, within EX31_RS01115 at 243.726 kb on - strand, within EX31_RS01115 at 243.726 kb on - strand, within EX31_RS01115 at 243.945 kb on + strand at 243.945 kb on + strand at 243.964 kb on - strand at 243.964 kb on - strand at 243.964 kb on - strand at 243.983 kb on - strand at 243.983 kb on - strand at 244.038 kb on + strand at 244.069 kb on + strand at 244.070 kb on - strand at 244.183 kb on + strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.184 kb on - strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.185 kb on + strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.186 kb on - strand at 244.187 kb on + strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.188 kb on - strand at 244.214 kb on - strand at 244.215 kb on + strand at 244.554 kb on - strand, within EX31_RS01120 at 244.563 kb on + strand, within EX31_RS01120 at 244.595 kb on + strand, within EX31_RS01120 at 244.782 kb on + strand, within EX31_RS01120 at 244.783 kb on - strand, within EX31_RS01120 at 244.783 kb on - strand, within EX31_RS01120 at 244.792 kb on - strand, within EX31_RS01120 at 244.860 kb on + strand, within EX31_RS01120
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 241,266 + +0.3 241,312 + +0.5 241,312 + -0.1 241,333 + +0.2 241,333 + +0.5 241,359 + +0.9 241,374 + +0.7 241,382 + +1.1 241,438 - +1.2 241,611 + EX31_RS01110 0.24 -0.1 241,611 + EX31_RS01110 0.24 +0.5 241,611 + EX31_RS01110 0.24 -1.1 241,612 - EX31_RS01110 0.24 -0.1 241,612 - EX31_RS01110 0.24 -0.2 241,657 + EX31_RS01110 0.30 -1.3 241,681 - EX31_RS01110 0.33 +0.4 241,686 + EX31_RS01110 0.34 -0.7 241,686 + EX31_RS01110 0.34 -0.8 241,687 - EX31_RS01110 0.34 -0.6 241,687 - EX31_RS01110 0.34 -0.6 241,688 + EX31_RS01110 0.34 +0.0 241,689 - EX31_RS01110 0.34 -1.0 241,689 - EX31_RS01110 0.34 -1.2 241,689 - EX31_RS01110 0.34 -1.6 241,969 + EX31_RS01110 0.73 -0.3 241,969 + EX31_RS01110 0.73 +1.2 241,969 + EX31_RS01110 0.73 +0.1 241,970 - EX31_RS01110 0.73 -0.7 241,970 - EX31_RS01110 0.73 +1.0 241,970 - EX31_RS01110 0.73 +0.6 241,970 - EX31_RS01110 0.73 -0.4 241,970 - EX31_RS01110 0.73 -1.6 241,971 + EX31_RS01110 0.73 +0.5 241,971 + EX31_RS01110 0.73 -0.6 241,971 + EX31_RS01110 0.73 +0.2 241,971 + EX31_RS01110 0.73 -2.1 241,971 + EX31_RS01110 0.73 -0.8 241,971 + EX31_RS01110 0.73 -0.8 241,972 - EX31_RS01110 0.73 -2.1 241,972 - EX31_RS01110 0.73 -0.4 241,972 - EX31_RS01110 0.73 +0.2 241,972 - EX31_RS01110 0.73 +2.0 241,993 + EX31_RS01110 0.76 +0.4 241,993 + EX31_RS01110 0.76 -0.8 242,203 + +1.1 242,203 + +0.1 242,208 - -0.8 242,218 - +1.3 242,220 - +1.0 242,230 - +0.4 242,234 + +0.2 242,234 + -0.6 242,235 - -0.3 242,235 - -0.6 242,235 - +2.3 242,235 - +0.4 242,235 - +0.9 242,235 - +0.2 242,235 - +0.4 242,242 + +0.2 242,243 - -0.1 242,243 - -0.6 242,474 - EX31_RS01115 0.13 +1.0 242,474 - EX31_RS01115 0.13 -0.1 242,658 + EX31_RS01115 0.24 +1.2 242,671 + EX31_RS01115 0.25 +0.3 242,672 - EX31_RS01115 0.25 +2.8 242,755 + EX31_RS01115 0.30 -0.4 242,872 + EX31_RS01115 0.36 +1.3 242,876 - EX31_RS01115 0.37 +0.9 242,933 + EX31_RS01115 0.40 -0.0 243,492 + EX31_RS01115 0.72 -0.8 243,493 - EX31_RS01115 0.72 -1.7 243,537 + EX31_RS01115 0.75 +0.4 243,725 + EX31_RS01115 0.86 -0.4 243,725 + EX31_RS01115 0.86 -0.5 243,726 - EX31_RS01115 0.86 +0.6 243,726 - EX31_RS01115 0.86 +1.2 243,726 - EX31_RS01115 0.86 +0.6 243,945 + +0.3 243,945 + -1.7 243,964 - +0.0 243,964 - +1.4 243,964 - +0.2 243,983 - +0.7 243,983 - -2.4 244,038 + +3.2 244,069 + +0.8 244,070 - -1.3 244,183 + -0.9 244,184 - +2.9 244,184 - -0.6 244,184 - +0.2 244,184 - +3.2 244,184 - -0.1 244,185 + +0.6 244,185 + -0.3 244,185 + -1.1 244,185 + -0.1 244,185 + -0.4 244,186 - +1.4 244,186 - +1.0 244,186 - +0.9 244,186 - -0.9 244,186 - +0.0 244,186 - +0.1 244,186 - -0.3 244,186 - +0.7 244,187 + +0.6 244,188 - -0.9 244,188 - -1.5 244,188 - +0.2 244,188 - +0.6 244,188 - -2.6 244,188 - -0.5 244,188 - -0.1 244,214 - +1.5 244,215 + -0.2 244,554 - EX31_RS01120 0.18 -1.5 244,563 + EX31_RS01120 0.19 -0.1 244,595 + EX31_RS01120 0.22 +1.2 244,782 + EX31_RS01120 0.37 -0.1 244,783 - EX31_RS01120 0.37 -0.8 244,783 - EX31_RS01120 0.37 +0.4 244,792 - EX31_RS01120 0.38 +0.6 244,860 + EX31_RS01120 0.43 -1.8
Or see this region's nucleotide sequence