Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07800 and MMP_RS07805 are separated by 14 nucleotides MMP_RS07805 and MMP_RS07810 are separated by 166 nucleotides MMP_RS07810 and MMP_RS07815 are separated by 26 nucleotides
MMP_RS07800: MMP_RS07800 - ATP-binding cassette domain-containing protein, at 1,477,790 to 1,478,728
_RS07800
MMP_RS07805: MMP_RS07805 - ABC transporter permease, at 1,478,743 to 1,479,510
_RS07805
MMP_RS07810: MMP_RS07810 - hydrogenase nickel incorporation protein HypB, at 1,479,677 to 1,480,351
_RS07810
MMP_RS07815: MMP_RS07815 - DUF2120 domain-containing protein, at 1,480,378 to 1,480,797
_RS07815
Position (kb)
1478
1479
1480 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1477.771 kb on + strand at 1477.786 kb on - strand at 1477.786 kb on - strand at 1477.819 kb on - strand at 1477.823 kb on + strand at 1478.178 kb on + strand, within MMP_RS07800 at 1478.237 kb on - strand, within MMP_RS07800 at 1478.237 kb on - strand, within MMP_RS07800 at 1478.339 kb on - strand, within MMP_RS07800 at 1478.339 kb on - strand, within MMP_RS07800 at 1478.340 kb on + strand, within MMP_RS07800 at 1478.363 kb on - strand, within MMP_RS07800 at 1478.364 kb on + strand, within MMP_RS07800 at 1478.364 kb on + strand, within MMP_RS07800 at 1478.366 kb on + strand, within MMP_RS07800 at 1478.366 kb on + strand, within MMP_RS07800 at 1478.543 kb on + strand, within MMP_RS07800 at 1478.594 kb on - strand, within MMP_RS07800 at 1478.704 kb on - strand at 1478.722 kb on - strand at 1478.722 kb on - strand at 1478.722 kb on - strand at 1478.732 kb on - strand at 1478.734 kb on - strand at 1478.745 kb on + strand at 1478.754 kb on + strand at 1478.754 kb on - strand at 1478.755 kb on + strand at 1478.757 kb on + strand at 1478.762 kb on + strand at 1478.791 kb on + strand at 1478.791 kb on + strand at 1478.809 kb on + strand at 1478.888 kb on - strand, within MMP_RS07805 at 1478.889 kb on + strand, within MMP_RS07805 at 1479.086 kb on - strand, within MMP_RS07805 at 1479.110 kb on + strand, within MMP_RS07805 at 1479.207 kb on - strand, within MMP_RS07805 at 1479.208 kb on + strand, within MMP_RS07805 at 1479.208 kb on + strand, within MMP_RS07805 at 1479.212 kb on + strand, within MMP_RS07805 at 1479.246 kb on - strand, within MMP_RS07805 at 1479.297 kb on + strand, within MMP_RS07805 at 1479.358 kb on - strand, within MMP_RS07805 at 1479.443 kb on + strand at 1479.534 kb on + strand at 1479.601 kb on - strand at 1479.605 kb on + strand at 1479.621 kb on - strand at 1479.640 kb on - strand at 1479.640 kb on - strand at 1479.641 kb on + strand at 1479.641 kb on + strand at 1479.641 kb on + strand at 1479.651 kb on - strand at 1479.652 kb on + strand at 1479.652 kb on + strand at 1479.714 kb on - strand at 1479.715 kb on + strand at 1480.003 kb on + strand at 1480.182 kb on - strand at 1480.276 kb on + strand at 1480.316 kb on + strand at 1480.336 kb on + strand at 1480.353 kb on - strand at 1480.355 kb on - strand at 1480.358 kb on + strand at 1480.358 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations remove 1,477,771 + -0.7 1,477,786 - -1.0 1,477,786 - -0.2 1,477,819 - -0.3 1,477,823 + +0.4 1,478,178 + MMP_RS07800 0.41 -5.0 1,478,237 - MMP_RS07800 0.48 -3.6 1,478,237 - MMP_RS07800 0.48 +0.8 1,478,339 - MMP_RS07800 0.58 +0.3 1,478,339 - MMP_RS07800 0.58 +1.2 1,478,340 + MMP_RS07800 0.59 -1.6 1,478,363 - MMP_RS07800 0.61 -3.7 1,478,364 + MMP_RS07800 0.61 +0.2 1,478,364 + MMP_RS07800 0.61 -1.8 1,478,366 + MMP_RS07800 0.61 -3.2 1,478,366 + MMP_RS07800 0.61 -0.1 1,478,543 + MMP_RS07800 0.80 +0.4 1,478,594 - MMP_RS07800 0.86 +0.1 1,478,704 - -1.9 1,478,722 - +1.1 1,478,722 - +0.7 1,478,722 - -0.6 1,478,732 - +1.1 1,478,734 - -0.2 1,478,745 + -2.0 1,478,754 + -0.2 1,478,754 - +0.5 1,478,755 + -1.2 1,478,757 + -0.4 1,478,762 + +0.9 1,478,791 + -0.9 1,478,791 + -1.9 1,478,809 + -5.2 1,478,888 - MMP_RS07805 0.19 -5.1 1,478,889 + MMP_RS07805 0.19 +0.8 1,479,086 - MMP_RS07805 0.45 +0.9 1,479,110 + MMP_RS07805 0.48 +2.0 1,479,207 - MMP_RS07805 0.60 +0.1 1,479,208 + MMP_RS07805 0.61 -0.8 1,479,208 + MMP_RS07805 0.61 -0.6 1,479,212 + MMP_RS07805 0.61 +1.0 1,479,246 - MMP_RS07805 0.65 -0.2 1,479,297 + MMP_RS07805 0.72 -1.6 1,479,358 - MMP_RS07805 0.80 -0.0 1,479,443 + -0.3 1,479,534 + -1.2 1,479,601 - +0.7 1,479,605 + +0.3 1,479,621 - -3.4 1,479,640 - -4.4 1,479,640 - -3.6 1,479,641 + +0.7 1,479,641 + -3.7 1,479,641 + -4.6 1,479,651 - -4.1 1,479,652 + -1.1 1,479,652 + -4.1 1,479,714 - -2.9 1,479,715 + -3.2 1,480,003 + -3.8 1,480,182 - -3.9 1,480,276 + -3.1 1,480,316 + +0.4 1,480,336 + +2.0 1,480,353 - -0.0 1,480,355 - -0.3 1,480,358 + -0.2 1,480,358 + -1.0
Or see this region's nucleotide sequence