Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06370 and MMP_RS06375 overlap by 4 nucleotides MMP_RS06375 and MMP_RS06380 are separated by 27 nucleotides
MMP_RS06370: MMP_RS06370 - biotin synthase BioB, at 1,223,883 to 1,224,866
_RS06370
MMP_RS06375: MMP_RS06375 - ATP-dependent sacrificial sulfur transferase LarE, at 1,224,863 to 1,225,639
_RS06375
MMP_RS06380: MMP_RS06380 - O-phospho-L-seryl-tRNA:Cys-tRNA synthase, at 1,225,667 to 1,226,809
_RS06380
Position (kb)
1224
1225
1226 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1224.010 kb on + strand, within MMP_RS06370 at 1224.221 kb on + strand, within MMP_RS06370 at 1224.364 kb on - strand, within MMP_RS06370 at 1224.417 kb on + strand, within MMP_RS06370 at 1224.448 kb on - strand, within MMP_RS06370 at 1224.577 kb on - strand, within MMP_RS06370 at 1224.897 kb on + strand at 1224.933 kb on - strand at 1224.971 kb on + strand, within MMP_RS06375 at 1224.971 kb on + strand, within MMP_RS06375 at 1225.246 kb on + strand, within MMP_RS06375 at 1225.378 kb on - strand, within MMP_RS06375 at 1225.485 kb on + strand, within MMP_RS06375 at 1225.570 kb on + strand at 1225.582 kb on - strand at 1225.902 kb on - strand at 1226.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations remove 1,224,010 + MMP_RS06370 0.13 +0.1 1,224,221 + MMP_RS06370 0.34 +1.4 1,224,364 - MMP_RS06370 0.49 +0.6 1,224,417 + MMP_RS06370 0.54 -4.2 1,224,448 - MMP_RS06370 0.57 +0.6 1,224,577 - MMP_RS06370 0.71 -1.1 1,224,897 + -0.6 1,224,933 - -1.1 1,224,971 + MMP_RS06375 0.14 -6.3 1,224,971 + MMP_RS06375 0.14 -4.4 1,225,246 + MMP_RS06375 0.49 -5.9 1,225,378 - MMP_RS06375 0.66 -4.5 1,225,485 + MMP_RS06375 0.80 -5.1 1,225,570 + -2.7 1,225,582 - -2.7 1,225,902 - -1.2 1,226,409 - -2.9
Or see this region's nucleotide sequence