Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06255 and MMP_RS06260 are separated by 123 nucleotides MMP_RS06260 and MMP_RS06265 are separated by 47 nucleotides MMP_RS06265 and MMP_RS06270 are separated by 17 nucleotides
MMP_RS06255: MMP_RS06255 - cobyric acid synthase CobQ, at 1,201,550 to 1,203,028
_RS06255
MMP_RS06260: MMP_RS06260 - histidinol-phosphate transaminase, at 1,203,152 to 1,204,267
_RS06260
MMP_RS06265: MMP_RS06265 - DUF2100 domain-containing protein, at 1,204,315 to 1,204,974
_RS06265
MMP_RS06270: MMP_RS06270 - TIGR00300 family protein, at 1,204,992 to 1,206,239
_RS06270
Position (kb)
1203
1204
1205 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1202.383 kb on - strand at 1203.109 kb on + strand at 1203.270 kb on + strand at 1204.595 kb on + strand at 1204.826 kb on + strand at 1204.974 kb on - strand at 1205.099 kb on - strand at 1205.118 kb on - strand, within MMP_RS06270 at 1205.140 kb on + strand, within MMP_RS06270 at 1205.144 kb on + strand, within MMP_RS06270 at 1205.222 kb on - strand, within MMP_RS06270 at 1205.250 kb on + strand, within MMP_RS06270
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations remove 1,202,383 - -3.0 1,203,109 + +2.0 1,203,270 + -2.0 1,204,595 + -3.1 1,204,826 + -2.1 1,204,974 - -2.4 1,205,099 - -3.1 1,205,118 - MMP_RS06270 0.10 -1.8 1,205,140 + MMP_RS06270 0.12 -3.9 1,205,144 + MMP_RS06270 0.12 -3.4 1,205,222 - MMP_RS06270 0.18 -4.7 1,205,250 + MMP_RS06270 0.21 -3.4
Or see this region's nucleotide sequence