Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05645 and MMP_RS05650 overlap by 4 nucleotides MMP_RS05650 and MMP_RS05655 are separated by 70 nucleotides MMP_RS05655 and MMP_RS05660 are separated by 243 nucleotides
MMP_RS05645: MMP_RS05645 - AEC family transporter, at 1,084,457 to 1,085,371
_RS05645
MMP_RS05650: MMP_RS05650 - phosphopantetheine adenylyltransferase, at 1,085,368 to 1,085,814
_RS05650
MMP_RS05655: MMP_RS05655 - phosphoenolpyruvate synthase, at 1,085,885 to 1,088,161
_RS05655
MMP_RS05660: MMP_RS05660 - phosphate ABC transporter substrate-binding protein, at 1,088,405 to 1,089,244
_RS05660
Position (kb)
1085
1086
1087
1088
1089 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1084.941 kb on - strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.978 kb on - strand, within MMP_RS05645 at 1085.158 kb on - strand, within MMP_RS05645 at 1085.166 kb on + strand, within MMP_RS05645 at 1085.212 kb on - strand, within MMP_RS05645 at 1085.215 kb on - strand, within MMP_RS05645 at 1085.349 kb on - strand at 1085.349 kb on - strand at 1085.349 kb on - strand at 1085.350 kb on + strand at 1085.350 kb on + strand at 1085.362 kb on - strand at 1085.552 kb on + strand at 1085.626 kb on + strand at 1085.712 kb on - strand at 1085.782 kb on + strand at 1085.790 kb on - strand at 1086.350 kb on + strand at 1086.907 kb on - strand at 1087.211 kb on + strand at 1087.469 kb on + strand at 1087.557 kb on + strand at 1087.557 kb on + strand at 1087.858 kb on + strand at 1087.918 kb on - strand at 1088.210 kb on - strand at 1088.243 kb on - strand at 1088.244 kb on + strand at 1088.245 kb on - strand at 1088.245 kb on - strand at 1088.246 kb on + strand at 1088.246 kb on + strand at 1088.247 kb on - strand at 1088.247 kb on - strand at 1088.248 kb on + strand at 1088.248 kb on + strand at 1088.283 kb on + strand at 1088.286 kb on + strand at 1088.418 kb on - strand at 1088.418 kb on - strand at 1088.419 kb on + strand at 1088.452 kb on + strand at 1088.492 kb on + strand, within MMP_RS05660 at 1088.516 kb on + strand, within MMP_RS05660 at 1088.536 kb on - strand, within MMP_RS05660 at 1088.598 kb on + strand, within MMP_RS05660 at 1088.758 kb on - strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.110 kb on - strand, within MMP_RS05660 at 1089.129 kb on - strand, within MMP_RS05660
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations remove 1,084,941 - MMP_RS05645 0.53 +0.1 1,084,942 + MMP_RS05645 0.53 +4.5 1,084,942 + MMP_RS05645 0.53 +1.1 1,084,942 + MMP_RS05645 0.53 +1.2 1,084,942 + MMP_RS05645 0.53 +3.0 1,084,978 - MMP_RS05645 0.57 +0.5 1,085,158 - MMP_RS05645 0.77 +0.8 1,085,166 + MMP_RS05645 0.77 +1.6 1,085,212 - MMP_RS05645 0.83 +0.5 1,085,215 - MMP_RS05645 0.83 +1.1 1,085,349 - +1.0 1,085,349 - +1.3 1,085,349 - -2.1 1,085,350 + +0.8 1,085,350 + +1.3 1,085,362 - -0.2 1,085,552 + -2.8 1,085,626 + -2.9 1,085,712 - -2.3 1,085,782 + -3.5 1,085,790 - -2.5 1,086,350 + -1.5 1,086,907 - -2.0 1,087,211 + -1.8 1,087,469 + -1.5 1,087,557 + -1.4 1,087,557 + -1.6 1,087,858 + -1.7 1,087,918 - -3.5 1,088,210 - -1.7 1,088,243 - -2.0 1,088,244 + -3.7 1,088,245 - -1.5 1,088,245 - +0.4 1,088,246 + -2.5 1,088,246 + -3.0 1,088,247 - -4.2 1,088,247 - -1.3 1,088,248 + -3.7 1,088,248 + -3.1 1,088,283 + +1.7 1,088,286 + -1.8 1,088,418 - +0.8 1,088,418 - -0.3 1,088,419 + +0.2 1,088,452 + +1.5 1,088,492 + MMP_RS05660 0.10 +0.5 1,088,516 + MMP_RS05660 0.13 +0.8 1,088,536 - MMP_RS05660 0.16 -4.5 1,088,598 + MMP_RS05660 0.23 +2.1 1,088,758 - MMP_RS05660 0.42 -0.5 1,089,097 + MMP_RS05660 0.82 -0.5 1,089,097 + MMP_RS05660 0.82 +0.6 1,089,097 + MMP_RS05660 0.82 +1.2 1,089,110 - MMP_RS05660 0.84 +1.5 1,089,129 - MMP_RS05660 0.86 +4.5
Or see this region's nucleotide sequence