Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03140 and MMP_RS03145 are separated by 17 nucleotides MMP_RS03145 and MMP_RS03150 are separated by 156 nucleotides MMP_RS03150 and MMP_RS03155 are separated by 4 nucleotides MMP_RS03155 and MMP_RS03160 are separated by 34 nucleotides
MMP_RS03140: MMP_RS03140 - hypothetical protein, at 591,868 to 593,298
_RS03140
MMP_RS03145: MMP_RS03145 - fibrillarin-like rRNA/tRNA 2'-O-methyltransferase, at 593,316 to 594,008
_RS03145
MMP_RS03150: MMP_RS03150 - phosphoglycerate mutase, at 594,165 to 595,451
_RS03150
MMP_RS03155: MMP_RS03155 - precorrin-6A reductase, at 595,456 to 596,202
_RS03155
MMP_RS03160: MMP_RS03160 - class III signal peptide-containing protein, at 596,237 to 596,920
_RS03160
Position (kb) 594
595
596 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 593.313 kb on - strand at 593.313 kb on - strand at 593.354 kb on + strand at 593.354 kb on + strand at 593.354 kb on + strand at 593.455 kb on - strand, within MMP_RS03145 at 593.455 kb on - strand, within MMP_RS03145 at 593.462 kb on - strand, within MMP_RS03145 at 593.504 kb on - strand, within MMP_RS03145 at 593.507 kb on + strand, within MMP_RS03145 at 593.554 kb on + strand, within MMP_RS03145 at 593.597 kb on - strand, within MMP_RS03145 at 594.006 kb on - strand at 594.007 kb on + strand at 594.016 kb on - strand at 594.016 kb on - strand at 594.017 kb on + strand at 594.023 kb on - strand at 594.059 kb on - strand at 594.068 kb on - strand at 594.069 kb on + strand at 594.069 kb on + strand at 594.071 kb on + strand at 594.091 kb on + strand at 594.103 kb on + strand at 594.121 kb on + strand at 594.135 kb on - strand at 594.144 kb on - strand at 594.148 kb on + strand at 594.327 kb on - strand, within MMP_RS03150 at 594.385 kb on - strand, within MMP_RS03150 at 594.438 kb on - strand, within MMP_RS03150 at 594.466 kb on - strand, within MMP_RS03150 at 594.477 kb on - strand, within MMP_RS03150 at 594.478 kb on + strand, within MMP_RS03150 at 594.494 kb on + strand, within MMP_RS03150 at 594.494 kb on + strand, within MMP_RS03150 at 594.498 kb on + strand, within MMP_RS03150 at 594.498 kb on + strand, within MMP_RS03150 at 594.687 kb on + strand, within MMP_RS03150 at 594.890 kb on - strand, within MMP_RS03150 at 595.243 kb on - strand, within MMP_RS03150 at 595.277 kb on - strand, within MMP_RS03150 at 595.308 kb on + strand, within MMP_RS03150 at 595.324 kb on - strand at 595.325 kb on + strand at 595.340 kb on + strand at 595.375 kb on - strand at 595.378 kb on - strand at 595.379 kb on + strand at 595.381 kb on + strand at 595.435 kb on - strand at 595.435 kb on - strand at 595.435 kb on - strand at 595.436 kb on + strand at 595.444 kb on - strand at 595.445 kb on + strand at 595.517 kb on + strand at 595.533 kb on - strand, within MMP_RS03155 at 595.612 kb on + strand, within MMP_RS03155 at 595.618 kb on - strand, within MMP_RS03155 at 595.619 kb on + strand, within MMP_RS03155 at 595.642 kb on - strand, within MMP_RS03155 at 595.643 kb on + strand, within MMP_RS03155 at 595.643 kb on + strand, within MMP_RS03155 at 595.807 kb on + strand, within MMP_RS03155 at 595.808 kb on - strand, within MMP_RS03155 at 595.808 kb on - strand, within MMP_RS03155 at 595.809 kb on + strand, within MMP_RS03155 at 595.814 kb on + strand, within MMP_RS03155 at 595.840 kb on - strand, within MMP_RS03155 at 595.916 kb on - strand, within MMP_RS03155 at 596.146 kb on + strand at 596.161 kb on + strand at 596.226 kb on + strand at 596.229 kb on - strand at 596.230 kb on + strand at 596.271 kb on + strand at 596.271 kb on + strand at 596.273 kb on + strand at 596.334 kb on + strand, within MMP_RS03160 at 596.360 kb on - strand, within MMP_RS03160
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations remove 593,313 - -0.8 593,313 - -1.0 593,354 + -2.2 593,354 + -1.8 593,354 + -0.4 593,455 - MMP_RS03145 0.20 -5.5 593,455 - MMP_RS03145 0.20 -0.3 593,462 - MMP_RS03145 0.21 -2.7 593,504 - MMP_RS03145 0.27 -1.0 593,507 + MMP_RS03145 0.28 -1.2 593,554 + MMP_RS03145 0.34 -1.1 593,597 - MMP_RS03145 0.41 -1.0 594,006 - +1.4 594,007 + +1.1 594,016 - +1.2 594,016 - +0.4 594,017 + +1.0 594,023 - +2.4 594,059 - +1.4 594,068 - +1.5 594,069 + +0.1 594,069 + +1.7 594,071 + +1.6 594,091 + +0.6 594,103 + +0.4 594,121 + +1.0 594,135 - +2.5 594,144 - +1.7 594,148 + +1.6 594,327 - MMP_RS03150 0.13 +2.2 594,385 - MMP_RS03150 0.17 +2.7 594,438 - MMP_RS03150 0.21 +2.2 594,466 - MMP_RS03150 0.23 +3.3 594,477 - MMP_RS03150 0.24 +1.4 594,478 + MMP_RS03150 0.24 +2.2 594,494 + MMP_RS03150 0.26 +1.8 594,494 + MMP_RS03150 0.26 +1.5 594,498 + MMP_RS03150 0.26 +2.2 594,498 + MMP_RS03150 0.26 +1.1 594,687 + MMP_RS03150 0.41 +1.4 594,890 - MMP_RS03150 0.56 +0.6 595,243 - MMP_RS03150 0.84 +1.7 595,277 - MMP_RS03150 0.86 +0.8 595,308 + MMP_RS03150 0.89 +1.5 595,324 - +2.5 595,325 + +2.3 595,340 + +1.8 595,375 - +2.8 595,378 - +2.1 595,379 + -0.2 595,381 + +0.9 595,435 - +0.4 595,435 - +1.4 595,435 - +1.1 595,436 + +2.3 595,444 - -0.1 595,445 + +1.5 595,517 + -2.3 595,533 - MMP_RS03155 0.10 -4.4 595,612 + MMP_RS03155 0.21 -4.7 595,618 - MMP_RS03155 0.22 -3.9 595,619 + MMP_RS03155 0.22 -3.4 595,642 - MMP_RS03155 0.25 -5.9 595,643 + MMP_RS03155 0.25 -4.4 595,643 + MMP_RS03155 0.25 -5.1 595,807 + MMP_RS03155 0.47 -4.1 595,808 - MMP_RS03155 0.47 -4.4 595,808 - MMP_RS03155 0.47 -3.7 595,809 + MMP_RS03155 0.47 -3.6 595,814 + MMP_RS03155 0.48 -4.6 595,840 - MMP_RS03155 0.51 -5.7 595,916 - MMP_RS03155 0.62 -4.0 596,146 + -4.5 596,161 + -3.6 596,226 + -3.7 596,229 - -0.8 596,230 + -4.1 596,271 + -5.7 596,271 + -2.2 596,273 + -1.9 596,334 + MMP_RS03160 0.14 -0.5 596,360 - MMP_RS03160 0.18 +0.9
Or see this region's nucleotide sequence