Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01045 and MMP_RS01050 are separated by 84 nucleotides MMP_RS01050 and MMP_RS01055 are separated by 334 nucleotides MMP_RS01055 and MMP_RS01060 are separated by 236 nucleotides
MMP_RS01045: MMP_RS01045 - diphthamide biosynthesis enzyme Dph2, at 196,599 to 197,528
_RS01045
MMP_RS01050: MMP_RS01050 - tRNA-Leu, at 197,613 to 197,697
_RS01050
MMP_RS01055: MMP_RS01055 - hypothetical protein, at 198,032 to 198,409
_RS01055
MMP_RS01060: MMP_RS01060 - calcium/sodium antiporter, at 198,646 to 199,608
_RS01060
Position (kb) 198
199 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 197.096 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.209 kb on - strand, within MMP_RS01045 at 197.322 kb on - strand, within MMP_RS01045 at 197.323 kb on + strand, within MMP_RS01045 at 197.325 kb on + strand, within MMP_RS01045 at 197.443 kb on - strand at 197.443 kb on - strand at 197.470 kb on + strand at 197.516 kb on - strand at 197.524 kb on + strand at 197.811 kb on - strand at 197.856 kb on - strand at 197.894 kb on - strand at 198.086 kb on - strand, within MMP_RS01055 at 198.086 kb on - strand, within MMP_RS01055 at 198.170 kb on + strand, within MMP_RS01055 at 198.406 kb on + strand at 198.406 kb on + strand at 198.411 kb on - strand at 198.411 kb on - strand at 198.412 kb on + strand at 198.444 kb on - strand at 198.569 kb on - strand at 198.605 kb on - strand at 198.640 kb on + strand at 198.644 kb on - strand at 198.820 kb on + strand, within MMP_RS01060 at 198.820 kb on + strand, within MMP_RS01060 at 198.903 kb on - strand, within MMP_RS01060 at 198.914 kb on + strand, within MMP_RS01060 at 198.930 kb on - strand, within MMP_RS01060 at 198.930 kb on - strand, within MMP_RS01060 at 198.930 kb on - strand, within MMP_RS01060 at 198.931 kb on + strand, within MMP_RS01060 at 198.959 kb on + strand, within MMP_RS01060 at 199.182 kb on - strand, within MMP_RS01060 at 199.182 kb on - strand, within MMP_RS01060 at 199.184 kb on - strand, within MMP_RS01060 at 199.184 kb on - strand, within MMP_RS01060 at 199.185 kb on + strand, within MMP_RS01060 at 199.186 kb on - strand, within MMP_RS01060 at 199.189 kb on - strand, within MMP_RS01060 at 199.197 kb on - strand, within MMP_RS01060 at 199.216 kb on - strand, within MMP_RS01060 at 199.283 kb on - strand, within MMP_RS01060 at 199.318 kb on + strand, within MMP_RS01060 at 199.345 kb on + strand, within MMP_RS01060 at 199.345 kb on + strand, within MMP_RS01060
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations remove 197,096 - MMP_RS01045 0.53 -2.6 197,209 - MMP_RS01045 0.66 -5.1 197,209 - MMP_RS01045 0.66 -2.7 197,322 - MMP_RS01045 0.78 -0.9 197,323 + MMP_RS01045 0.78 +0.4 197,325 + MMP_RS01045 0.78 -2.0 197,443 - -4.9 197,443 - -1.7 197,470 + -1.4 197,516 - -1.6 197,524 + +0.9 197,811 - -4.6 197,856 - +1.1 197,894 - -0.5 198,086 - MMP_RS01055 0.14 +0.6 198,086 - MMP_RS01055 0.14 +1.9 198,170 + MMP_RS01055 0.37 +2.6 198,406 + +0.5 198,406 + -0.3 198,411 - +1.3 198,411 - -0.8 198,412 + -0.8 198,444 - -0.6 198,569 - -0.2 198,605 - -2.0 198,640 + +0.3 198,644 - -0.5 198,820 + MMP_RS01060 0.18 -0.6 198,820 + MMP_RS01060 0.18 +1.1 198,903 - MMP_RS01060 0.27 +1.2 198,914 + MMP_RS01060 0.28 +0.7 198,930 - MMP_RS01060 0.29 +0.6 198,930 - MMP_RS01060 0.29 +1.4 198,930 - MMP_RS01060 0.29 +0.4 198,931 + MMP_RS01060 0.30 -3.9 198,959 + MMP_RS01060 0.33 -0.3 199,182 - MMP_RS01060 0.56 +0.5 199,182 - MMP_RS01060 0.56 -1.0 199,184 - MMP_RS01060 0.56 +0.0 199,184 - MMP_RS01060 0.56 +1.4 199,185 + MMP_RS01060 0.56 -0.2 199,186 - MMP_RS01060 0.56 -0.2 199,189 - MMP_RS01060 0.56 +0.7 199,197 - MMP_RS01060 0.57 -2.6 199,216 - MMP_RS01060 0.59 +0.3 199,283 - MMP_RS01060 0.66 -0.1 199,318 + MMP_RS01060 0.70 +0.6 199,345 + MMP_RS01060 0.73 +0.4 199,345 + MMP_RS01060 0.73 +0.6
Or see this region's nucleotide sequence