Experiment: Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00970 and MMP_RS00975 overlap by 4 nucleotides MMP_RS00975 and MMP_RS00980 are separated by 142 nucleotides MMP_RS00980 and MMP_RS00985 are separated by 24 nucleotides
MMP_RS00970: MMP_RS00970 - coenzyme F430 synthase, at 180,497 to 181,699
_RS00970
MMP_RS00975: MMP_RS00975 - HD domain-containing protein, at 181,696 to 183,072
_RS00975
MMP_RS00980: MMP_RS00980 - hypothetical protein, at 183,215 to 183,679
_RS00980
MMP_RS00985: MMP_RS00985 - CDC48 family AAA ATPase, at 183,704 to 186,070
_RS00985
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 180.924 kb on - strand at 180.933 kb on - strand at 181.086 kb on - strand at 181.086 kb on - strand at 181.149 kb on - strand at 181.151 kb on - strand at 181.152 kb on + strand at 181.291 kb on + strand at 181.406 kb on + strand at 181.434 kb on - strand at 181.464 kb on - strand at 181.482 kb on - strand at 181.501 kb on + strand at 181.621 kb on - strand at 181.636 kb on + strand at 181.666 kb on - strand at 181.731 kb on - strand at 181.883 kb on + strand, within MMP_RS00975 at 181.896 kb on - strand, within MMP_RS00975 at 182.019 kb on + strand, within MMP_RS00975 at 182.091 kb on - strand, within MMP_RS00975 at 182.092 kb on + strand, within MMP_RS00975 at 182.132 kb on + strand, within MMP_RS00975 at 182.198 kb on + strand, within MMP_RS00975 at 182.281 kb on - strand, within MMP_RS00975 at 182.293 kb on + strand, within MMP_RS00975 at 182.328 kb on - strand, within MMP_RS00975 at 182.328 kb on - strand, within MMP_RS00975 at 182.329 kb on + strand, within MMP_RS00975 at 182.332 kb on - strand, within MMP_RS00975 at 182.359 kb on - strand, within MMP_RS00975 at 182.399 kb on + strand, within MMP_RS00975 at 182.579 kb on + strand, within MMP_RS00975 at 182.621 kb on + strand, within MMP_RS00975 at 182.696 kb on + strand, within MMP_RS00975 at 182.714 kb on - strand, within MMP_RS00975 at 182.715 kb on + strand, within MMP_RS00975 at 182.782 kb on - strand, within MMP_RS00975 at 182.895 kb on - strand, within MMP_RS00975 at 182.950 kb on + strand at 183.001 kb on - strand at 183.007 kb on - strand at 183.022 kb on + strand at 183.031 kb on + strand at 183.082 kb on - strand at 183.096 kb on - strand at 183.114 kb on - strand at 183.114 kb on - strand at 183.160 kb on - strand at 183.160 kb on - strand at 183.161 kb on + strand at 183.164 kb on - strand at 183.164 kb on - strand at 183.165 kb on + strand at 183.165 kb on + strand at 183.172 kb on + strand at 183.192 kb on - strand at 183.198 kb on - strand at 183.198 kb on - strand at 183.198 kb on - strand at 183.236 kb on - strand at 183.248 kb on + strand at 183.248 kb on + strand at 183.344 kb on - strand, within MMP_RS00980 at 183.345 kb on + strand, within MMP_RS00980 at 183.345 kb on + strand, within MMP_RS00980 at 183.350 kb on - strand, within MMP_RS00980 at 183.351 kb on + strand, within MMP_RS00980 at 183.370 kb on - strand, within MMP_RS00980 at 183.371 kb on + strand, within MMP_RS00980 at 183.467 kb on - strand, within MMP_RS00980 at 183.540 kb on - strand, within MMP_RS00980 at 183.552 kb on - strand, within MMP_RS00980 at 183.611 kb on - strand, within MMP_RS00980 at 183.611 kb on - strand, within MMP_RS00980 at 183.612 kb on + strand, within MMP_RS00980 at 183.613 kb on - strand, within MMP_RS00980 at 183.613 kb on - strand, within MMP_RS00980 at 183.613 kb on - strand, within MMP_RS00980 at 183.614 kb on + strand, within MMP_RS00980 at 183.618 kb on - strand, within MMP_RS00980 at 183.619 kb on + strand, within MMP_RS00980 at 183.619 kb on + strand, within MMP_RS00980 at 183.634 kb on - strand at 183.689 kb on + strand at 183.689 kb on + strand at 183.800 kb on + strand at 183.813 kb on + strand at 183.923 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations remove 180,924 - -2.9 180,933 - -3.3 181,086 - -3.7 181,086 - -3.2 181,149 - -3.6 181,151 - -3.3 181,152 + -4.6 181,291 + -3.9 181,406 + -3.5 181,434 - -3.3 181,464 - -4.3 181,482 - -3.7 181,501 + -1.1 181,621 - -2.4 181,636 + -2.9 181,666 - -2.7 181,731 - -3.0 181,883 + MMP_RS00975 0.14 +2.0 181,896 - MMP_RS00975 0.15 +1.2 182,019 + MMP_RS00975 0.23 +2.2 182,091 - MMP_RS00975 0.29 +0.2 182,092 + MMP_RS00975 0.29 +0.7 182,132 + MMP_RS00975 0.32 +2.3 182,198 + MMP_RS00975 0.36 +2.1 182,281 - MMP_RS00975 0.42 +1.9 182,293 + MMP_RS00975 0.43 +1.9 182,328 - MMP_RS00975 0.46 +2.0 182,328 - MMP_RS00975 0.46 +0.8 182,329 + MMP_RS00975 0.46 +2.0 182,332 - MMP_RS00975 0.46 +2.2 182,359 - MMP_RS00975 0.48 +2.6 182,399 + MMP_RS00975 0.51 +0.9 182,579 + MMP_RS00975 0.64 +0.7 182,621 + MMP_RS00975 0.67 +1.3 182,696 + MMP_RS00975 0.73 +1.5 182,714 - MMP_RS00975 0.74 +1.8 182,715 + MMP_RS00975 0.74 +2.2 182,782 - MMP_RS00975 0.79 +1.8 182,895 - MMP_RS00975 0.87 +1.0 182,950 + +2.7 183,001 - +0.6 183,007 - +1.9 183,022 + +1.7 183,031 + +1.2 183,082 - +2.8 183,096 - +2.7 183,114 - -5.1 183,114 - +0.1 183,160 - +0.3 183,160 - -0.3 183,161 + +1.9 183,164 - +1.2 183,164 - -0.5 183,165 + +1.8 183,165 + -1.1 183,172 + -0.0 183,192 - -0.5 183,198 - +0.9 183,198 - +0.0 183,198 - +0.7 183,236 - +0.1 183,248 + +2.2 183,248 + -5.3 183,344 - MMP_RS00980 0.28 -0.2 183,345 + MMP_RS00980 0.28 -1.3 183,345 + MMP_RS00980 0.28 +0.0 183,350 - MMP_RS00980 0.29 +1.1 183,351 + MMP_RS00980 0.29 +0.4 183,370 - MMP_RS00980 0.33 -0.7 183,371 + MMP_RS00980 0.34 +0.8 183,467 - MMP_RS00980 0.54 +1.5 183,540 - MMP_RS00980 0.70 +1.3 183,552 - MMP_RS00980 0.72 +0.9 183,611 - MMP_RS00980 0.85 +0.3 183,611 - MMP_RS00980 0.85 +0.1 183,612 + MMP_RS00980 0.85 +0.6 183,613 - MMP_RS00980 0.86 +0.2 183,613 - MMP_RS00980 0.86 +1.7 183,613 - MMP_RS00980 0.86 +0.9 183,614 + MMP_RS00980 0.86 +0.5 183,618 - MMP_RS00980 0.87 -0.5 183,619 + MMP_RS00980 0.87 +1.4 183,619 + MMP_RS00980 0.87 -0.5 183,634 - -2.9 183,689 + +0.7 183,689 + +0.2 183,800 + +0.9 183,813 + -6.1 183,923 - -4.6
Or see this region's nucleotide sequence