Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24615 and EX31_RS24620 are separated by 127 nucleotides EX31_RS24620 and EX31_RS24625 are separated by 201 nucleotides EX31_RS24625 and EX31_RS24630 are separated by 63 nucleotides EX31_RS24630 and EX31_RS24635 are separated by 183 nucleotides
EX31_RS24615: EX31_RS24615 - peptide ABC transporter substrate-binding protein, at 691,913 to 693,553
_RS24615
EX31_RS24620: EX31_RS24620 - YebW family protein, at 693,681 to 693,872
_RS24620
EX31_RS24625: EX31_RS24625 - DUF2766 domain-containing protein, at 694,074 to 694,319
_RS24625
EX31_RS24630: EX31_RS24630 - membrane protein, at 694,383 to 694,619
_RS24630
EX31_RS24635: EX31_RS24635 - autotransporter outer membrane beta-barrel domain-containing protein, at 694,803 to 697,496
_RS24635
Position (kb) 694
695 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 693.107 kb on + strand, within EX31_RS24615 at 693.107 kb on + strand, within EX31_RS24615 at 693.108 kb on - strand, within EX31_RS24615 at 693.108 kb on - strand, within EX31_RS24615 at 693.108 kb on - strand, within EX31_RS24615 at 693.108 kb on - strand, within EX31_RS24615 at 693.115 kb on + strand, within EX31_RS24615 at 693.115 kb on + strand, within EX31_RS24615 at 693.116 kb on - strand, within EX31_RS24615 at 693.116 kb on - strand, within EX31_RS24615 at 693.116 kb on - strand, within EX31_RS24615 at 693.116 kb on - strand, within EX31_RS24615 at 693.121 kb on + strand, within EX31_RS24615 at 693.121 kb on + strand, within EX31_RS24615 at 693.122 kb on - strand, within EX31_RS24615 at 693.122 kb on - strand, within EX31_RS24615 at 693.122 kb on - strand, within EX31_RS24615 at 693.122 kb on - strand, within EX31_RS24615 at 693.126 kb on + strand, within EX31_RS24615 at 693.127 kb on - strand, within EX31_RS24615 at 693.127 kb on - strand, within EX31_RS24615 at 693.127 kb on - strand, within EX31_RS24615 at 693.158 kb on + strand, within EX31_RS24615 at 693.159 kb on - strand, within EX31_RS24615 at 693.263 kb on - strand, within EX31_RS24615 at 693.307 kb on + strand, within EX31_RS24615 at 693.307 kb on + strand, within EX31_RS24615 at 693.308 kb on - strand, within EX31_RS24615 at 693.308 kb on - strand, within EX31_RS24615 at 693.309 kb on + strand, within EX31_RS24615 at 693.311 kb on + strand, within EX31_RS24615 at 693.311 kb on + strand, within EX31_RS24615 at 693.311 kb on + strand, within EX31_RS24615 at 693.311 kb on + strand, within EX31_RS24615 at 693.311 kb on + strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.312 kb on - strand, within EX31_RS24615 at 693.314 kb on - strand, within EX31_RS24615 at 693.314 kb on - strand, within EX31_RS24615 at 693.465 kb on + strand at 693.582 kb on - strand at 693.803 kb on + strand, within EX31_RS24620 at 693.804 kb on - strand, within EX31_RS24620 at 693.819 kb on + strand, within EX31_RS24620 at 693.820 kb on - strand, within EX31_RS24620 at 693.845 kb on - strand, within EX31_RS24620 at 693.852 kb on - strand, within EX31_RS24620 at 693.873 kb on - strand at 693.900 kb on + strand at 693.900 kb on + strand at 693.901 kb on - strand at 693.906 kb on + strand at 693.906 kb on + strand at 693.906 kb on + strand at 693.906 kb on + strand at 693.907 kb on - strand at 693.907 kb on - strand at 693.907 kb on - strand at 693.907 kb on - strand at 693.907 kb on - strand at 693.907 kb on - strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.912 kb on + strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.913 kb on - strand at 693.915 kb on + strand at 693.916 kb on - strand at 693.916 kb on - strand at 693.916 kb on - strand at 693.916 kb on - strand at 693.916 kb on - strand at 693.954 kb on + strand at 693.955 kb on - strand at 694.024 kb on - strand at 694.135 kb on + strand, within EX31_RS24625 at 694.136 kb on - strand, within EX31_RS24625 at 694.166 kb on + strand, within EX31_RS24625 at 694.167 kb on - strand, within EX31_RS24625 at 694.301 kb on + strand at 694.301 kb on + strand at 694.320 kb on + strand at 694.320 kb on + strand at 694.324 kb on + strand at 694.324 kb on + strand at 694.325 kb on - strand at 694.358 kb on - strand at 694.358 kb on - strand at 694.358 kb on - strand at 694.363 kb on - strand at 694.376 kb on - strand at 694.384 kb on + strand at 694.388 kb on + strand at 694.388 kb on + strand at 694.463 kb on + strand, within EX31_RS24630 at 694.463 kb on + strand, within EX31_RS24630 at 694.464 kb on - strand, within EX31_RS24630 at 694.464 kb on - strand, within EX31_RS24630 at 694.464 kb on - strand, within EX31_RS24630 at 694.466 kb on - strand, within EX31_RS24630 at 694.476 kb on + strand, within EX31_RS24630 at 694.476 kb on + strand, within EX31_RS24630 at 694.477 kb on - strand, within EX31_RS24630 at 694.495 kb on - strand, within EX31_RS24630 at 694.495 kb on - strand, within EX31_RS24630 at 694.579 kb on + strand, within EX31_RS24630 at 694.579 kb on + strand, within EX31_RS24630 at 694.580 kb on - strand, within EX31_RS24630 at 694.625 kb on - strand at 694.633 kb on - strand at 694.666 kb on + strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.667 kb on - strand at 694.670 kb on + strand at 694.670 kb on + strand at 694.670 kb on + strand at 694.670 kb on + strand at 694.670 kb on + strand at 694.670 kb on + strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.671 kb on - strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.672 kb on + strand at 694.673 kb on - strand at 694.673 kb on - strand at 694.676 kb on - strand at 694.676 kb on - strand at 694.716 kb on - strand at 694.804 kb on + strand at 694.867 kb on + strand at 694.868 kb on - strand at 694.868 kb on - strand at 694.868 kb on - strand at 694.872 kb on - strand at 695.002 kb on + strand at 695.003 kb on - strand at 695.003 kb on - strand at 695.003 kb on - strand at 695.003 kb on - strand at 695.008 kb on + strand at 695.009 kb on - strand at 695.009 kb on - strand at 695.009 kb on - strand at 695.009 kb on - strand at 695.009 kb on - strand at 695.009 kb on - strand at 695.030 kb on + strand at 695.031 kb on - strand at 695.060 kb on + strand at 695.060 kb on + strand at 695.061 kb on - strand at 695.061 kb on - strand at 695.061 kb on - strand at 695.061 kb on - strand at 695.063 kb on - strand at 695.101 kb on + strand, within EX31_RS24635 at 695.102 kb on - strand, within EX31_RS24635 at 695.140 kb on + strand, within EX31_RS24635 at 695.169 kb on - strand, within EX31_RS24635 at 695.169 kb on - strand, within EX31_RS24635 at 695.268 kb on + strand, within EX31_RS24635 at 695.269 kb on - strand, within EX31_RS24635
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 693,107 + EX31_RS24615 0.73 +1.9 693,107 + EX31_RS24615 0.73 -1.3 693,108 - EX31_RS24615 0.73 +0.6 693,108 - EX31_RS24615 0.73 -1.7 693,108 - EX31_RS24615 0.73 +1.1 693,108 - EX31_RS24615 0.73 -1.0 693,115 + EX31_RS24615 0.73 -0.9 693,115 + EX31_RS24615 0.73 +0.1 693,116 - EX31_RS24615 0.73 +1.3 693,116 - EX31_RS24615 0.73 +1.9 693,116 - EX31_RS24615 0.73 +0.2 693,116 - EX31_RS24615 0.73 +1.2 693,121 + EX31_RS24615 0.74 +1.9 693,121 + EX31_RS24615 0.74 -0.5 693,122 - EX31_RS24615 0.74 -0.6 693,122 - EX31_RS24615 0.74 -0.8 693,122 - EX31_RS24615 0.74 -0.6 693,122 - EX31_RS24615 0.74 +0.1 693,126 + EX31_RS24615 0.74 -0.1 693,127 - EX31_RS24615 0.74 +2.2 693,127 - EX31_RS24615 0.74 +0.9 693,127 - EX31_RS24615 0.74 +1.6 693,158 + EX31_RS24615 0.76 -0.2 693,159 - EX31_RS24615 0.76 -0.4 693,263 - EX31_RS24615 0.82 -0.4 693,307 + EX31_RS24615 0.85 +1.4 693,307 + EX31_RS24615 0.85 +0.9 693,308 - EX31_RS24615 0.85 -0.3 693,308 - EX31_RS24615 0.85 +1.3 693,309 + EX31_RS24615 0.85 +0.3 693,311 + EX31_RS24615 0.85 -1.5 693,311 + EX31_RS24615 0.85 -0.3 693,311 + EX31_RS24615 0.85 -0.4 693,311 + EX31_RS24615 0.85 -0.1 693,311 + EX31_RS24615 0.85 +2.9 693,312 - EX31_RS24615 0.85 +0.5 693,312 - EX31_RS24615 0.85 +0.5 693,312 - EX31_RS24615 0.85 -0.1 693,312 - EX31_RS24615 0.85 +1.7 693,312 - EX31_RS24615 0.85 +0.3 693,312 - EX31_RS24615 0.85 -0.5 693,312 - EX31_RS24615 0.85 +0.7 693,312 - EX31_RS24615 0.85 +0.8 693,312 - EX31_RS24615 0.85 +1.2 693,312 - EX31_RS24615 0.85 +0.9 693,314 - EX31_RS24615 0.85 -0.0 693,314 - EX31_RS24615 0.85 -0.4 693,465 + -0.1 693,582 - +0.3 693,803 + EX31_RS24620 0.64 -0.7 693,804 - EX31_RS24620 0.64 +0.1 693,819 + EX31_RS24620 0.72 +0.4 693,820 - EX31_RS24620 0.72 +0.8 693,845 - EX31_RS24620 0.85 +0.2 693,852 - EX31_RS24620 0.89 +1.3 693,873 - +0.3 693,900 + -0.3 693,900 + -0.9 693,901 - -1.3 693,906 + -1.0 693,906 + +0.5 693,906 + +0.1 693,906 + -1.5 693,907 - -0.3 693,907 - +1.1 693,907 - -0.2 693,907 - -0.1 693,907 - -0.4 693,907 - -1.0 693,912 + +3.9 693,912 + +0.1 693,912 + -0.9 693,912 + +0.4 693,912 + +1.1 693,912 + +0.2 693,912 + +0.9 693,912 + -1.4 693,912 + -0.6 693,912 + +0.1 693,912 + +1.9 693,913 - +0.1 693,913 - -0.9 693,913 - -0.9 693,913 - +1.6 693,913 - -1.3 693,913 - +0.5 693,913 - +1.1 693,913 - +0.8 693,913 - -0.5 693,913 - -0.4 693,913 - +1.9 693,913 - +0.9 693,913 - +0.1 693,913 - +1.4 693,913 - -0.1 693,915 + +1.3 693,916 - +0.1 693,916 - +1.2 693,916 - -0.5 693,916 - +0.9 693,916 - +0.7 693,954 + +0.4 693,955 - -1.4 694,024 - +0.5 694,135 + EX31_RS24625 0.25 +0.3 694,136 - EX31_RS24625 0.25 -0.2 694,166 + EX31_RS24625 0.37 -0.8 694,167 - EX31_RS24625 0.38 -0.4 694,301 + -0.3 694,301 + -1.8 694,320 + +1.9 694,320 + +0.5 694,324 + +0.3 694,324 + +1.3 694,325 - -0.9 694,358 - -0.8 694,358 - -0.5 694,358 - +0.2 694,363 - -1.3 694,376 - +1.2 694,384 + +0.9 694,388 + +0.7 694,388 + +0.9 694,463 + EX31_RS24630 0.34 -0.4 694,463 + EX31_RS24630 0.34 +0.2 694,464 - EX31_RS24630 0.34 +0.8 694,464 - EX31_RS24630 0.34 +0.5 694,464 - EX31_RS24630 0.34 -0.4 694,466 - EX31_RS24630 0.35 +1.4 694,476 + EX31_RS24630 0.39 -0.6 694,476 + EX31_RS24630 0.39 -1.4 694,477 - EX31_RS24630 0.40 +1.2 694,495 - EX31_RS24630 0.47 -0.9 694,495 - EX31_RS24630 0.47 -0.1 694,579 + EX31_RS24630 0.83 -2.4 694,579 + EX31_RS24630 0.83 -0.4 694,580 - EX31_RS24630 0.83 +0.6 694,625 - +0.0 694,633 - -0.9 694,666 + +1.9 694,667 - -0.0 694,667 - -0.0 694,667 - -1.7 694,667 - +1.0 694,667 - -0.6 694,670 + +0.0 694,670 + -0.6 694,670 + +1.0 694,670 + -0.5 694,670 + -0.4 694,670 + +0.3 694,671 - +1.5 694,671 - -0.4 694,671 - +0.3 694,671 - -0.9 694,671 - -0.9 694,671 - +1.1 694,671 - -1.1 694,671 - -1.1 694,671 - +1.2 694,672 + +0.4 694,672 + +1.9 694,672 + -0.4 694,673 - -0.3 694,673 - +0.2 694,676 - -1.1 694,676 - +0.1 694,716 - -0.5 694,804 + -0.3 694,867 + +1.3 694,868 - +0.9 694,868 - -0.1 694,868 - -0.5 694,872 - +0.0 695,002 + +0.9 695,003 - -0.4 695,003 - +0.9 695,003 - +1.6 695,003 - +2.9 695,008 + +0.7 695,009 - +1.6 695,009 - +0.7 695,009 - -0.1 695,009 - -0.3 695,009 - +0.3 695,009 - +0.3 695,030 + +0.7 695,031 - -0.1 695,060 + +0.3 695,060 + +1.2 695,061 - +1.0 695,061 - +0.5 695,061 - +0.0 695,061 - -1.5 695,063 - +0.3 695,101 + EX31_RS24635 0.11 -0.2 695,102 - EX31_RS24635 0.11 +0.4 695,140 + EX31_RS24635 0.13 +0.7 695,169 - EX31_RS24635 0.14 +1.2 695,169 - EX31_RS24635 0.14 +0.6 695,268 + EX31_RS24635 0.17 -0.3 695,269 - EX31_RS24635 0.17 -0.4
Or see this region's nucleotide sequence