Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24400 and EX31_RS24405 are separated by 49 nucleotides EX31_RS24405 and EX31_RS24410 are separated by 142 nucleotides EX31_RS24410 and EX31_RS24415 are separated by 12 nucleotides EX31_RS24415 and EX31_RS24420 are separated by 48 nucleotides
EX31_RS24400: EX31_RS24400 - 23S rRNA pseudouridine(955/2504/2580) synthase RluC, at 649,808 to 650,770
_RS24400
EX31_RS24405: EX31_RS24405 - septum formation inhibitor Maf, at 650,820 to 651,413
_RS24405
EX31_RS24410: EX31_RS24410 - 23S rRNA accumulation protein YceD, at 651,556 to 652,080
_RS24410
EX31_RS24415: EX31_RS24415 - 50S ribosomal protein L32, at 652,093 to 652,260
_RS24415
EX31_RS24420: EX31_RS24420 - phosphate acyltransferase PlsX, at 652,309 to 653,343
_RS24420
Position (kb) 651
652
653 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 650.588 kb on + strand, within EX31_RS24400 at 650.640 kb on + strand, within EX31_RS24400 at 650.698 kb on + strand at 650.698 kb on + strand at 650.699 kb on - strand at 650.719 kb on + strand at 650.719 kb on + strand at 650.719 kb on + strand at 650.720 kb on - strand at 650.745 kb on + strand at 650.768 kb on + strand at 650.768 kb on + strand at 650.768 kb on + strand at 650.779 kb on + strand at 650.782 kb on + strand at 650.821 kb on + strand at 650.821 kb on + strand at 650.822 kb on - strand at 650.822 kb on - strand at 650.822 kb on - strand at 650.822 kb on - strand at 650.824 kb on - strand at 650.824 kb on - strand at 650.824 kb on - strand at 650.870 kb on + strand at 650.949 kb on - strand, within EX31_RS24405 at 650.968 kb on + strand, within EX31_RS24405 at 650.968 kb on + strand, within EX31_RS24405 at 650.968 kb on + strand, within EX31_RS24405 at 650.968 kb on + strand, within EX31_RS24405 at 650.968 kb on + strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 650.969 kb on - strand, within EX31_RS24405 at 651.013 kb on - strand, within EX31_RS24405 at 651.045 kb on + strand, within EX31_RS24405 at 651.045 kb on + strand, within EX31_RS24405 at 651.046 kb on - strand, within EX31_RS24405 at 651.046 kb on - strand, within EX31_RS24405 at 651.046 kb on - strand, within EX31_RS24405 at 651.046 kb on - strand, within EX31_RS24405 at 651.076 kb on + strand, within EX31_RS24405 at 651.076 kb on + strand, within EX31_RS24405 at 651.076 kb on + strand, within EX31_RS24405 at 651.076 kb on + strand, within EX31_RS24405 at 651.076 kb on + strand, within EX31_RS24405 at 651.076 kb on + strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.077 kb on - strand, within EX31_RS24405 at 651.094 kb on + strand, within EX31_RS24405 at 651.094 kb on + strand, within EX31_RS24405 at 651.095 kb on - strand, within EX31_RS24405 at 651.095 kb on - strand, within EX31_RS24405 at 651.095 kb on - strand, within EX31_RS24405 at 651.334 kb on - strand, within EX31_RS24405 at 651.433 kb on + strand at 651.434 kb on - strand at 651.504 kb on + strand at 651.541 kb on + strand at 651.544 kb on + strand at 651.619 kb on + strand, within EX31_RS24410 at 651.619 kb on + strand, within EX31_RS24410 at 651.621 kb on + strand, within EX31_RS24410 at 651.631 kb on + strand, within EX31_RS24410 at 651.631 kb on + strand, within EX31_RS24410 at 651.631 kb on + strand, within EX31_RS24410 at 651.631 kb on + strand, within EX31_RS24410 at 651.633 kb on + strand, within EX31_RS24410 at 651.633 kb on + strand, within EX31_RS24410 at 651.633 kb on + strand, within EX31_RS24410 at 651.633 kb on + strand, within EX31_RS24410 at 651.687 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.689 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.817 kb on + strand, within EX31_RS24410 at 651.825 kb on + strand, within EX31_RS24410 at 651.871 kb on + strand, within EX31_RS24410 at 651.936 kb on + strand, within EX31_RS24410 at 651.962 kb on + strand, within EX31_RS24410 at 651.962 kb on + strand, within EX31_RS24410 at 652.004 kb on + strand, within EX31_RS24410 at 652.074 kb on + strand at 652.074 kb on + strand at 652.078 kb on + strand at 652.100 kb on + strand at 652.125 kb on + strand, within EX31_RS24415 at 652.250 kb on + strand at 652.261 kb on + strand at 652.276 kb on + strand at 652.276 kb on + strand at 652.276 kb on + strand at 652.287 kb on + strand at 652.319 kb on + strand at 652.331 kb on + strand at 652.331 kb on + strand at 652.331 kb on + strand at 652.337 kb on + strand at 652.337 kb on + strand at 652.337 kb on + strand at 652.337 kb on + strand at 652.337 kb on + strand at 652.337 kb on + strand at 652.337 kb on + strand at 652.338 kb on - strand at 652.398 kb on + strand at 652.409 kb on + strand at 652.409 kb on + strand at 652.409 kb on + strand at 652.512 kb on + strand, within EX31_RS24420 at 652.514 kb on + strand, within EX31_RS24420 at 652.514 kb on + strand, within EX31_RS24420 at 652.526 kb on + strand, within EX31_RS24420 at 652.526 kb on + strand, within EX31_RS24420 at 652.542 kb on + strand, within EX31_RS24420 at 652.554 kb on + strand, within EX31_RS24420 at 652.649 kb on + strand, within EX31_RS24420 at 652.709 kb on + strand, within EX31_RS24420 at 652.709 kb on + strand, within EX31_RS24420 at 652.709 kb on + strand, within EX31_RS24420 at 652.713 kb on + strand, within EX31_RS24420 at 652.725 kb on + strand, within EX31_RS24420 at 652.728 kb on + strand, within EX31_RS24420 at 652.728 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 652.939 kb on + strand, within EX31_RS24420 at 653.042 kb on + strand, within EX31_RS24420 at 653.042 kb on + strand, within EX31_RS24420 at 653.042 kb on + strand, within EX31_RS24420 at 653.042 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420 at 653.045 kb on + strand, within EX31_RS24420
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 650,588 + EX31_RS24400 0.81 +1.4 650,640 + EX31_RS24400 0.86 +1.2 650,698 + -0.8 650,698 + +0.9 650,699 - -0.1 650,719 + +1.7 650,719 + -2.3 650,719 + -0.1 650,720 - -1.0 650,745 + -0.3 650,768 + +1.5 650,768 + +0.3 650,768 + +0.3 650,779 + +0.2 650,782 + -0.1 650,821 + +1.7 650,821 + -0.1 650,822 - -0.4 650,822 - -0.6 650,822 - -1.7 650,822 - -1.8 650,824 - +0.6 650,824 - +0.9 650,824 - +0.3 650,870 + +0.5 650,949 - EX31_RS24405 0.22 +0.1 650,968 + EX31_RS24405 0.25 -0.8 650,968 + EX31_RS24405 0.25 +0.1 650,968 + EX31_RS24405 0.25 -0.1 650,968 + EX31_RS24405 0.25 +0.2 650,968 + EX31_RS24405 0.25 +0.3 650,969 - EX31_RS24405 0.25 +0.8 650,969 - EX31_RS24405 0.25 -0.3 650,969 - EX31_RS24405 0.25 +0.0 650,969 - EX31_RS24405 0.25 +1.1 650,969 - EX31_RS24405 0.25 +0.3 650,969 - EX31_RS24405 0.25 -0.6 650,969 - EX31_RS24405 0.25 +1.3 650,969 - EX31_RS24405 0.25 +0.5 651,013 - EX31_RS24405 0.32 -0.1 651,045 + EX31_RS24405 0.38 +0.7 651,045 + EX31_RS24405 0.38 +0.3 651,046 - EX31_RS24405 0.38 +3.5 651,046 - EX31_RS24405 0.38 +0.3 651,046 - EX31_RS24405 0.38 +1.7 651,046 - EX31_RS24405 0.38 -0.8 651,076 + EX31_RS24405 0.43 +1.1 651,076 + EX31_RS24405 0.43 +0.9 651,076 + EX31_RS24405 0.43 +0.2 651,076 + EX31_RS24405 0.43 -1.3 651,076 + EX31_RS24405 0.43 +2.4 651,076 + EX31_RS24405 0.43 +1.0 651,077 - EX31_RS24405 0.43 +2.9 651,077 - EX31_RS24405 0.43 -2.3 651,077 - EX31_RS24405 0.43 -0.5 651,077 - EX31_RS24405 0.43 -0.9 651,077 - EX31_RS24405 0.43 -1.3 651,077 - EX31_RS24405 0.43 -0.1 651,077 - EX31_RS24405 0.43 +2.1 651,077 - EX31_RS24405 0.43 +0.1 651,077 - EX31_RS24405 0.43 +0.7 651,077 - EX31_RS24405 0.43 +0.7 651,094 + EX31_RS24405 0.46 -1.3 651,094 + EX31_RS24405 0.46 -0.4 651,095 - EX31_RS24405 0.46 +0.9 651,095 - EX31_RS24405 0.46 +0.6 651,095 - EX31_RS24405 0.46 +0.2 651,334 - EX31_RS24405 0.87 +1.1 651,433 + +0.5 651,434 - -0.9 651,504 + +1.2 651,541 + -1.3 651,544 + -1.1 651,619 + EX31_RS24410 0.12 +0.6 651,619 + EX31_RS24410 0.12 -0.9 651,621 + EX31_RS24410 0.12 -0.6 651,631 + EX31_RS24410 0.14 +0.1 651,631 + EX31_RS24410 0.14 -1.2 651,631 + EX31_RS24410 0.14 -0.7 651,631 + EX31_RS24410 0.14 -1.3 651,633 + EX31_RS24410 0.15 -0.9 651,633 + EX31_RS24410 0.15 -0.3 651,633 + EX31_RS24410 0.15 +0.3 651,633 + EX31_RS24410 0.15 -1.3 651,687 + EX31_RS24410 0.25 +0.3 651,689 + EX31_RS24410 0.25 -1.8 651,689 + EX31_RS24410 0.25 -2.3 651,689 + EX31_RS24410 0.25 -1.1 651,689 + EX31_RS24410 0.25 -2.0 651,689 + EX31_RS24410 0.25 +0.4 651,689 + EX31_RS24410 0.25 +1.3 651,689 + EX31_RS24410 0.25 -0.1 651,689 + EX31_RS24410 0.25 -1.1 651,689 + EX31_RS24410 0.25 +0.5 651,689 + EX31_RS24410 0.25 +0.1 651,689 + EX31_RS24410 0.25 -1.6 651,817 + EX31_RS24410 0.50 -0.7 651,817 + EX31_RS24410 0.50 -0.4 651,817 + EX31_RS24410 0.50 -0.2 651,817 + EX31_RS24410 0.50 -2.4 651,817 + EX31_RS24410 0.50 -0.7 651,817 + EX31_RS24410 0.50 -1.1 651,817 + EX31_RS24410 0.50 -0.4 651,817 + EX31_RS24410 0.50 -1.1 651,817 + EX31_RS24410 0.50 -1.0 651,825 + EX31_RS24410 0.51 +1.4 651,871 + EX31_RS24410 0.60 -3.1 651,936 + EX31_RS24410 0.72 -2.7 651,962 + EX31_RS24410 0.77 +0.7 651,962 + EX31_RS24410 0.77 -2.1 652,004 + EX31_RS24410 0.85 +0.1 652,074 + +0.6 652,074 + -0.3 652,078 + -0.4 652,100 + +0.3 652,125 + EX31_RS24415 0.19 -1.1 652,250 + -0.1 652,261 + -0.4 652,276 + -0.8 652,276 + -0.5 652,276 + +0.9 652,287 + +0.9 652,319 + -0.1 652,331 + +0.3 652,331 + +0.9 652,331 + -0.5 652,337 + -0.9 652,337 + -2.4 652,337 + +0.1 652,337 + +0.9 652,337 + -0.9 652,337 + -0.1 652,337 + -2.1 652,338 - -1.5 652,398 + +0.9 652,409 + -0.9 652,409 + -2.1 652,409 + -0.2 652,512 + EX31_RS24420 0.20 -0.9 652,514 + EX31_RS24420 0.20 +0.2 652,514 + EX31_RS24420 0.20 -0.7 652,526 + EX31_RS24420 0.21 -1.5 652,526 + EX31_RS24420 0.21 +0.7 652,542 + EX31_RS24420 0.23 -0.2 652,554 + EX31_RS24420 0.24 +0.5 652,649 + EX31_RS24420 0.33 -1.0 652,709 + EX31_RS24420 0.39 -0.6 652,709 + EX31_RS24420 0.39 -0.9 652,709 + EX31_RS24420 0.39 +0.2 652,713 + EX31_RS24420 0.39 -0.5 652,725 + EX31_RS24420 0.40 -2.0 652,728 + EX31_RS24420 0.40 +0.1 652,728 + EX31_RS24420 0.40 -2.0 652,939 + EX31_RS24420 0.61 -0.3 652,939 + EX31_RS24420 0.61 -0.2 652,939 + EX31_RS24420 0.61 -0.1 652,939 + EX31_RS24420 0.61 -0.7 652,939 + EX31_RS24420 0.61 -0.4 652,939 + EX31_RS24420 0.61 +0.4 652,939 + EX31_RS24420 0.61 +0.2 652,939 + EX31_RS24420 0.61 -0.7 652,939 + EX31_RS24420 0.61 -0.1 652,939 + EX31_RS24420 0.61 -0.7 652,939 + EX31_RS24420 0.61 +0.4 653,042 + EX31_RS24420 0.71 -1.1 653,042 + EX31_RS24420 0.71 -0.5 653,042 + EX31_RS24420 0.71 +0.6 653,042 + EX31_RS24420 0.71 -0.1 653,045 + EX31_RS24420 0.71 -0.9 653,045 + EX31_RS24420 0.71 +1.3 653,045 + EX31_RS24420 0.71 +0.9 653,045 + EX31_RS24420 0.71 -0.1 653,045 + EX31_RS24420 0.71 -0.7 653,045 + EX31_RS24420 0.71 -0.1 653,045 + EX31_RS24420 0.71 -0.1 653,045 + EX31_RS24420 0.71 -0.3
Or see this region's nucleotide sequence