Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS24255 and EX31_RS24260 are separated by 208 nucleotides EX31_RS24260 and EX31_RS24265 are separated by 6 nucleotides EX31_RS24265 and EX31_RS24270 are separated by 66 nucleotides EX31_RS24270 and EX31_RS24275 are separated by 245 nucleotides EX31_RS24275 and EX31_RS24280 are separated by 171 nucleotides
EX31_RS24255: EX31_RS24255 - glucans biosynthesis glucosyltransferase MdoH, at 618,406 to 620,988
_RS24255
EX31_RS24260: EX31_RS24260 - YceK/YidQ family lipoprotein, at 621,197 to 621,442
_RS24260
EX31_RS24265: EX31_RS24265 - secY/secA suppressor protein, at 621,449 to 621,856
_RS24265
EX31_RS24270: EX31_RS24270 - hypothetical protein, at 621,923 to 622,207
_RS24270
EX31_RS24275: EX31_RS24275 - hypothetical protein, at 622,453 to 622,647
_RS24275
EX31_RS24280: EX31_RS24280 - hypothetical protein, at 622,819 to 623,283
_RS24280
Position (kb) 621
622
623 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 620.931 kb on - strand at 620.931 kb on - strand at 620.976 kb on + strand at 620.977 kb on - strand at 620.977 kb on - strand at 621.021 kb on + strand at 621.034 kb on - strand at 621.034 kb on - strand at 621.073 kb on + strand at 621.131 kb on - strand at 621.211 kb on + strand at 621.272 kb on - strand, within EX31_RS24260 at 621.314 kb on + strand, within EX31_RS24260 at 621.434 kb on + strand at 621.440 kb on + strand at 621.446 kb on - strand at 621.451 kb on - strand at 621.509 kb on - strand, within EX31_RS24265 at 621.543 kb on + strand, within EX31_RS24265 at 621.544 kb on - strand, within EX31_RS24265 at 621.565 kb on - strand, within EX31_RS24265 at 621.565 kb on - strand, within EX31_RS24265 at 621.565 kb on - strand, within EX31_RS24265 at 621.602 kb on + strand, within EX31_RS24265 at 621.603 kb on - strand, within EX31_RS24265 at 621.628 kb on + strand, within EX31_RS24265 at 621.628 kb on + strand, within EX31_RS24265 at 621.732 kb on + strand, within EX31_RS24265 at 621.733 kb on - strand, within EX31_RS24265 at 621.827 kb on - strand at 621.847 kb on - strand at 621.847 kb on - strand at 621.856 kb on + strand at 621.857 kb on - strand at 621.895 kb on + strand at 621.896 kb on - strand at 621.951 kb on + strand at 621.954 kb on - strand, within EX31_RS24270 at 621.961 kb on + strand, within EX31_RS24270 at 621.961 kb on + strand, within EX31_RS24270 at 622.017 kb on + strand, within EX31_RS24270 at 622.017 kb on + strand, within EX31_RS24270 at 622.045 kb on - strand, within EX31_RS24270 at 622.062 kb on - strand, within EX31_RS24270 at 622.083 kb on - strand, within EX31_RS24270 at 622.104 kb on + strand, within EX31_RS24270 at 622.119 kb on - strand, within EX31_RS24270 at 622.119 kb on - strand, within EX31_RS24270 at 622.138 kb on + strand, within EX31_RS24270 at 622.287 kb on - strand at 622.346 kb on - strand at 622.346 kb on - strand at 622.368 kb on - strand at 622.451 kb on + strand at 622.468 kb on + strand at 622.663 kb on + strand at 622.769 kb on + strand at 622.819 kb on - strand at 622.849 kb on + strand at 622.914 kb on + strand, within EX31_RS24280 at 622.914 kb on + strand, within EX31_RS24280 at 622.915 kb on - strand, within EX31_RS24280 at 622.958 kb on + strand, within EX31_RS24280 at 622.958 kb on + strand, within EX31_RS24280 at 622.959 kb on - strand, within EX31_RS24280 at 623.019 kb on + strand, within EX31_RS24280 at 623.032 kb on + strand, within EX31_RS24280 at 623.040 kb on + strand, within EX31_RS24280 at 623.040 kb on + strand, within EX31_RS24280 at 623.052 kb on + strand, within EX31_RS24280 at 623.115 kb on + strand, within EX31_RS24280 at 623.116 kb on - strand, within EX31_RS24280 at 623.148 kb on + strand, within EX31_RS24280 at 623.148 kb on + strand, within EX31_RS24280 at 623.148 kb on + strand, within EX31_RS24280 at 623.149 kb on - strand, within EX31_RS24280 at 623.149 kb on - strand, within EX31_RS24280 at 623.190 kb on + strand, within EX31_RS24280 at 623.194 kb on - strand, within EX31_RS24280 at 623.199 kb on - strand, within EX31_RS24280 at 623.199 kb on - strand, within EX31_RS24280
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 620,931 - -1.1 620,931 - -0.5 620,976 + -0.8 620,977 - +0.7 620,977 - -0.1 621,021 + -1.2 621,034 - +0.7 621,034 - +0.3 621,073 + -0.5 621,131 - -0.1 621,211 + +2.5 621,272 - EX31_RS24260 0.30 -1.9 621,314 + EX31_RS24260 0.48 -0.0 621,434 + +1.0 621,440 + +1.4 621,446 - -0.3 621,451 - -2.1 621,509 - EX31_RS24265 0.15 +0.1 621,543 + EX31_RS24265 0.23 -0.1 621,544 - EX31_RS24265 0.23 +1.3 621,565 - EX31_RS24265 0.28 +0.0 621,565 - EX31_RS24265 0.28 +0.3 621,565 - EX31_RS24265 0.28 +0.6 621,602 + EX31_RS24265 0.38 +0.5 621,603 - EX31_RS24265 0.38 -0.2 621,628 + EX31_RS24265 0.44 +2.9 621,628 + EX31_RS24265 0.44 -1.5 621,732 + EX31_RS24265 0.69 -0.6 621,733 - EX31_RS24265 0.70 -0.1 621,827 - +0.8 621,847 - -1.5 621,847 - -1.9 621,856 + -0.3 621,857 - +0.8 621,895 + +1.9 621,896 - +0.2 621,951 + +1.5 621,954 - EX31_RS24270 0.11 +0.8 621,961 + EX31_RS24270 0.13 +2.5 621,961 + EX31_RS24270 0.13 +1.4 622,017 + EX31_RS24270 0.33 +0.2 622,017 + EX31_RS24270 0.33 +1.2 622,045 - EX31_RS24270 0.43 +1.9 622,062 - EX31_RS24270 0.49 -0.9 622,083 - EX31_RS24270 0.56 +0.1 622,104 + EX31_RS24270 0.64 +0.1 622,119 - EX31_RS24270 0.69 -0.5 622,119 - EX31_RS24270 0.69 +0.9 622,138 + EX31_RS24270 0.75 +1.2 622,287 - -0.2 622,346 - -0.5 622,346 - -0.9 622,368 - -0.2 622,451 + -0.7 622,468 + -1.8 622,663 + -0.2 622,769 + -0.7 622,819 - +0.5 622,849 + +1.1 622,914 + EX31_RS24280 0.20 +0.4 622,914 + EX31_RS24280 0.20 +0.8 622,915 - EX31_RS24280 0.21 +0.7 622,958 + EX31_RS24280 0.30 +0.5 622,958 + EX31_RS24280 0.30 +0.2 622,959 - EX31_RS24280 0.30 +0.9 623,019 + EX31_RS24280 0.43 -0.3 623,032 + EX31_RS24280 0.46 -1.3 623,040 + EX31_RS24280 0.48 +0.3 623,040 + EX31_RS24280 0.48 +0.5 623,052 + EX31_RS24280 0.50 -0.4 623,115 + EX31_RS24280 0.64 +1.2 623,116 - EX31_RS24280 0.64 +0.6 623,148 + EX31_RS24280 0.71 +0.3 623,148 + EX31_RS24280 0.71 +1.2 623,148 + EX31_RS24280 0.71 -0.4 623,149 - EX31_RS24280 0.71 -0.7 623,149 - EX31_RS24280 0.71 -0.7 623,190 + EX31_RS24280 0.80 +0.3 623,194 - EX31_RS24280 0.81 +1.9 623,199 - EX31_RS24280 0.82 +0.6 623,199 - EX31_RS24280 0.82 +0.1
Or see this region's nucleotide sequence