Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25665 and EX31_RS24220 are separated by 400 nucleotides EX31_RS24220 and EX31_RS24225 are separated by 77 nucleotides EX31_RS24225 and EX31_RS24230 are separated by 18 nucleotides
EX31_RS25665: EX31_RS25665 - DUF2502 domain-containing protein, at 609,971 to 610,336
_RS25665
EX31_RS24220: EX31_RS24220 - hypothetical protein, at 610,737 to 610,997
_RS24220
EX31_RS24225: EX31_RS24225 - universal stress protein, at 611,075 to 611,521
_RS24225
EX31_RS24230: EX31_RS24230 - IclR family transcriptional regulator, at 611,540 to 612,334
_RS24230
Position (kb) 610
611 Strain fitness (log2 ratio) -2
-1
0
1
2 at 609.770 kb on - strand at 609.780 kb on + strand at 609.823 kb on + strand at 609.868 kb on - strand at 609.868 kb on - strand at 609.987 kb on + strand at 609.987 kb on + strand at 610.051 kb on + strand, within EX31_RS25665 at 610.051 kb on + strand, within EX31_RS25665 at 610.051 kb on + strand, within EX31_RS25665 at 610.100 kb on + strand, within EX31_RS25665 at 610.100 kb on + strand, within EX31_RS25665 at 610.209 kb on - strand, within EX31_RS25665 at 610.475 kb on + strand at 610.475 kb on + strand at 610.476 kb on - strand at 610.520 kb on - strand at 610.520 kb on - strand at 610.582 kb on + strand at 610.582 kb on + strand at 610.582 kb on + strand at 610.661 kb on + strand at 610.661 kb on + strand at 610.661 kb on + strand at 610.661 kb on + strand at 610.662 kb on - strand at 610.662 kb on - strand at 610.662 kb on - strand at 610.662 kb on - strand at 610.683 kb on + strand at 610.684 kb on - strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.705 kb on + strand at 610.706 kb on - strand at 610.706 kb on - strand at 610.706 kb on - strand at 610.710 kb on - strand at 610.710 kb on - strand at 610.794 kb on + strand, within EX31_RS24220 at 610.808 kb on - strand, within EX31_RS24220 at 610.984 kb on + strand at 610.984 kb on + strand at 610.991 kb on + strand at 610.995 kb on + strand at 610.996 kb on - strand at 611.021 kb on + strand at 611.074 kb on - strand at 611.076 kb on + strand at 611.077 kb on - strand at 611.381 kb on - strand, within EX31_RS24225 at 611.542 kb on - strand at 611.575 kb on + strand at 611.667 kb on - strand, within EX31_RS24230 at 611.695 kb on + strand, within EX31_RS24230 at 611.696 kb on - strand, within EX31_RS24230 at 611.699 kb on + strand, within EX31_RS24230 at 611.699 kb on + strand, within EX31_RS24230 at 611.700 kb on - strand, within EX31_RS24230 at 611.700 kb on - strand, within EX31_RS24230 at 611.849 kb on - strand, within EX31_RS24230 at 611.874 kb on - strand, within EX31_RS24230 at 611.908 kb on - strand, within EX31_RS24230
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 609,770 - +0.3 609,780 + +0.3 609,823 + +1.4 609,868 - -1.1 609,868 - -0.2 609,987 + +0.7 609,987 + +0.8 610,051 + EX31_RS25665 0.22 -1.4 610,051 + EX31_RS25665 0.22 -1.1 610,051 + EX31_RS25665 0.22 +0.6 610,100 + EX31_RS25665 0.35 +0.9 610,100 + EX31_RS25665 0.35 +0.3 610,209 - EX31_RS25665 0.65 +0.5 610,475 + +0.7 610,475 + +0.6 610,476 - -0.5 610,520 - +0.8 610,520 - -0.2 610,582 + -1.4 610,582 + +0.3 610,582 + -0.0 610,661 + +1.9 610,661 + +1.6 610,661 + +1.2 610,661 + +0.9 610,662 - +0.3 610,662 - +0.3 610,662 - +1.1 610,662 - -1.4 610,683 + +0.9 610,684 - +0.6 610,705 + -1.4 610,705 + +0.3 610,705 + +0.9 610,705 + +0.8 610,705 + +0.1 610,705 + +0.4 610,706 - -1.5 610,706 - +1.5 610,706 - +1.3 610,710 - +0.1 610,710 - +0.3 610,794 + EX31_RS24220 0.22 -2.0 610,808 - EX31_RS24220 0.27 -1.3 610,984 + +0.5 610,984 + +0.9 610,991 + +0.9 610,995 + -1.3 610,996 - +0.8 611,021 + +0.3 611,074 - +0.3 611,076 + +0.3 611,077 - +0.2 611,381 - EX31_RS24225 0.68 -0.8 611,542 - +0.5 611,575 + +0.8 611,667 - EX31_RS24230 0.16 -0.0 611,695 + EX31_RS24230 0.19 -1.1 611,696 - EX31_RS24230 0.20 +1.0 611,699 + EX31_RS24230 0.20 -0.3 611,699 + EX31_RS24230 0.20 +1.3 611,700 - EX31_RS24230 0.20 +0.1 611,700 - EX31_RS24230 0.20 -1.7 611,849 - EX31_RS24230 0.39 -0.6 611,874 - EX31_RS24230 0.42 -0.3 611,908 - EX31_RS24230 0.46 +0.1
Or see this region's nucleotide sequence