Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21840 and EX31_RS21845 are separated by 11 nucleotides EX31_RS21845 and EX31_RS21850 are separated by 103 nucleotides EX31_RS21850 and EX31_RS21855 overlap by 1 nucleotides EX31_RS21855 and EX31_RS21860 are separated by 34 nucleotides EX31_RS21860 and EX31_RS21865 are separated by 113 nucleotides
EX31_RS21840: EX31_RS21840 - GTPase Era, at 111,487 to 112,392
_RS21840
EX31_RS21845: EX31_RS21845 - DNA repair protein RecO, at 112,404 to 113,141
_RS21845
EX31_RS21850: EX31_RS21850 - pyridoxine 5'-phosphate synthase, at 113,245 to 113,976
_RS21850
EX31_RS21855: EX31_RS21855 - holo-ACP synthase, at 113,976 to 114,356
_RS21855
EX31_RS21860: EX31_RS21860 - YfhL family 4Fe-4S dicluster ferredoxin, at 114,391 to 114,645
_RS21860
EX31_RS21865: EX31_RS21865 - MurR/RpiR family transcriptional regulator, at 114,759 to 115,607
_RS21865
Position (kb)
113
114 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 112.379 kb on + strand at 112.379 kb on + strand at 112.379 kb on + strand at 112.380 kb on - strand at 112.388 kb on + strand at 112.388 kb on + strand at 112.388 kb on + strand at 112.388 kb on + strand at 112.388 kb on + strand at 112.388 kb on + strand at 112.388 kb on + strand at 112.388 kb on + strand at 112.390 kb on + strand at 112.390 kb on + strand at 112.390 kb on + strand at 112.391 kb on - strand at 112.391 kb on - strand at 112.404 kb on - strand at 112.446 kb on + strand at 112.446 kb on + strand at 112.446 kb on + strand at 112.447 kb on - strand at 112.463 kb on - strand at 112.504 kb on + strand, within EX31_RS21845 at 112.562 kb on + strand, within EX31_RS21845 at 112.563 kb on - strand, within EX31_RS21845 at 112.563 kb on - strand, within EX31_RS21845 at 112.570 kb on + strand, within EX31_RS21845 at 112.571 kb on - strand, within EX31_RS21845 at 112.613 kb on + strand, within EX31_RS21845 at 112.675 kb on - strand, within EX31_RS21845 at 112.715 kb on + strand, within EX31_RS21845 at 112.715 kb on + strand, within EX31_RS21845 at 112.716 kb on - strand, within EX31_RS21845 at 112.716 kb on - strand, within EX31_RS21845 at 112.716 kb on - strand, within EX31_RS21845 at 112.720 kb on - strand, within EX31_RS21845 at 112.720 kb on - strand, within EX31_RS21845 at 112.740 kb on + strand, within EX31_RS21845 at 112.741 kb on - strand, within EX31_RS21845 at 112.819 kb on + strand, within EX31_RS21845 at 112.819 kb on + strand, within EX31_RS21845 at 112.819 kb on + strand, within EX31_RS21845 at 112.940 kb on + strand, within EX31_RS21845 at 112.940 kb on + strand, within EX31_RS21845 at 113.050 kb on - strand, within EX31_RS21845 at 113.140 kb on - strand at 113.157 kb on - strand at 113.180 kb on + strand at 113.213 kb on + strand at 113.213 kb on + strand at 113.214 kb on - strand at 113.229 kb on + strand at 113.229 kb on + strand at 113.271 kb on + strand at 113.271 kb on + strand at 113.314 kb on + strand at 113.314 kb on + strand at 113.418 kb on - strand, within EX31_RS21850 at 113.655 kb on + strand, within EX31_RS21850 at 113.695 kb on + strand, within EX31_RS21850 at 113.719 kb on + strand, within EX31_RS21850 at 113.719 kb on + strand, within EX31_RS21850 at 113.785 kb on + strand, within EX31_RS21850 at 113.924 kb on + strand at 113.936 kb on + strand at 114.346 kb on + strand at 114.346 kb on + strand at 114.354 kb on + strand at 114.354 kb on + strand at 114.355 kb on - strand at 114.355 kb on - strand at 114.355 kb on - strand at 114.355 kb on - strand at 114.357 kb on + strand at 114.358 kb on - strand at 114.358 kb on - strand at 114.358 kb on - strand at 114.358 kb on - strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.360 kb on + strand at 114.364 kb on + strand at 114.392 kb on + strand at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.471 kb on + strand, within EX31_RS21860 at 114.472 kb on - strand, within EX31_RS21860 at 114.472 kb on - strand, within EX31_RS21860 at 114.472 kb on - strand, within EX31_RS21860 at 114.508 kb on - strand, within EX31_RS21860 at 114.512 kb on + strand, within EX31_RS21860 at 114.512 kb on + strand, within EX31_RS21860 at 114.513 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.607 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.616 kb on - strand, within EX31_RS21860 at 114.658 kb on + strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.659 kb on - strand at 114.668 kb on - strand at 114.680 kb on - strand at 114.696 kb on - strand at 114.696 kb on - strand at 114.716 kb on + strand at 114.819 kb on - strand at 114.907 kb on - strand, within EX31_RS21865 at 114.920 kb on + strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.921 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.957 kb on - strand, within EX31_RS21865 at 114.972 kb on - strand, within EX31_RS21865
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 112,379 + -0.0 112,379 + +0.7 112,379 + +0.3 112,380 - -1.4 112,388 + -2.1 112,388 + +0.1 112,388 + +0.2 112,388 + +0.4 112,388 + +0.2 112,388 + -0.1 112,388 + -1.0 112,388 + -0.8 112,390 + +0.3 112,390 + -1.7 112,390 + -0.7 112,391 - -0.8 112,391 - +0.2 112,404 - +0.1 112,446 + +0.2 112,446 + -0.5 112,446 + +1.0 112,447 - +0.0 112,463 - -1.0 112,504 + EX31_RS21845 0.14 +1.3 112,562 + EX31_RS21845 0.21 +0.3 112,563 - EX31_RS21845 0.22 +0.4 112,563 - EX31_RS21845 0.22 -0.1 112,570 + EX31_RS21845 0.22 -0.5 112,571 - EX31_RS21845 0.23 -3.0 112,613 + EX31_RS21845 0.28 +0.4 112,675 - EX31_RS21845 0.37 +0.8 112,715 + EX31_RS21845 0.42 -0.7 112,715 + EX31_RS21845 0.42 -0.4 112,716 - EX31_RS21845 0.42 -0.2 112,716 - EX31_RS21845 0.42 -0.4 112,716 - EX31_RS21845 0.42 +0.1 112,720 - EX31_RS21845 0.43 +0.5 112,720 - EX31_RS21845 0.43 +0.2 112,740 + EX31_RS21845 0.46 -0.6 112,741 - EX31_RS21845 0.46 +0.3 112,819 + EX31_RS21845 0.56 -1.6 112,819 + EX31_RS21845 0.56 +0.1 112,819 + EX31_RS21845 0.56 +0.5 112,940 + EX31_RS21845 0.73 +0.0 112,940 + EX31_RS21845 0.73 +2.0 113,050 - EX31_RS21845 0.88 -0.5 113,140 - -0.3 113,157 - -0.7 113,180 + +0.2 113,213 + +0.4 113,213 + +0.4 113,214 - -1.3 113,229 + -0.2 113,229 + -1.1 113,271 + -2.1 113,271 + -1.8 113,314 + -1.4 113,314 + -1.7 113,418 - EX31_RS21850 0.24 -0.6 113,655 + EX31_RS21850 0.56 -0.3 113,695 + EX31_RS21850 0.61 -0.3 113,719 + EX31_RS21850 0.65 -1.4 113,719 + EX31_RS21850 0.65 -1.9 113,785 + EX31_RS21850 0.74 -2.0 113,924 + -1.9 113,936 + -3.0 114,346 + +1.1 114,346 + -0.1 114,354 + -0.3 114,354 + -0.0 114,355 - +1.2 114,355 - +1.0 114,355 - +0.6 114,355 - +0.6 114,357 + +1.0 114,358 - -0.1 114,358 - +1.0 114,358 - -1.4 114,358 - +0.6 114,360 + +0.6 114,360 + +1.0 114,360 + +0.8 114,360 + +0.1 114,360 + +0.3 114,360 + -0.4 114,360 + +0.7 114,364 + -1.4 114,392 + +1.0 114,471 + EX31_RS21860 0.31 -0.4 114,471 + EX31_RS21860 0.31 +0.5 114,471 + EX31_RS21860 0.31 -0.3 114,471 + EX31_RS21860 0.31 +0.3 114,471 + EX31_RS21860 0.31 -1.4 114,471 + EX31_RS21860 0.31 +0.3 114,472 - EX31_RS21860 0.32 -0.4 114,472 - EX31_RS21860 0.32 -1.3 114,472 - EX31_RS21860 0.32 +0.6 114,508 - EX31_RS21860 0.46 -0.1 114,512 + EX31_RS21860 0.47 -0.7 114,512 + EX31_RS21860 0.47 +0.3 114,513 - EX31_RS21860 0.48 -0.2 114,607 - EX31_RS21860 0.85 -0.6 114,607 - EX31_RS21860 0.85 +0.1 114,607 - EX31_RS21860 0.85 -0.6 114,607 - EX31_RS21860 0.85 -0.8 114,616 - EX31_RS21860 0.88 +0.3 114,616 - EX31_RS21860 0.88 -0.8 114,616 - EX31_RS21860 0.88 -1.2 114,616 - EX31_RS21860 0.88 +0.9 114,658 + +0.2 114,659 - +0.3 114,659 - -1.6 114,659 - +0.1 114,659 - +0.0 114,659 - +1.8 114,668 - +0.0 114,680 - -0.6 114,696 - +1.0 114,696 - -0.8 114,716 + +2.8 114,819 - -0.3 114,907 - EX31_RS21865 0.17 +0.8 114,920 + EX31_RS21865 0.19 -0.3 114,921 - EX31_RS21865 0.19 +0.2 114,921 - EX31_RS21865 0.19 +0.0 114,921 - EX31_RS21865 0.19 -2.1 114,921 - EX31_RS21865 0.19 -1.8 114,921 - EX31_RS21865 0.19 +0.1 114,957 - EX31_RS21865 0.23 -0.3 114,957 - EX31_RS21865 0.23 +0.3 114,957 - EX31_RS21865 0.23 -0.3 114,957 - EX31_RS21865 0.23 -0.6 114,957 - EX31_RS21865 0.23 +0.4 114,957 - EX31_RS21865 0.23 +0.2 114,957 - EX31_RS21865 0.23 -0.9 114,972 - EX31_RS21865 0.25 -0.3
Or see this region's nucleotide sequence