Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20970 and EX31_RS20975 are separated by 65 nucleotides EX31_RS20975 and EX31_RS20980 are separated by 10 nucleotides
EX31_RS20970: EX31_RS20970 - LysR family transcriptional regulator, at 837,002 to 837,895
_RS20970
EX31_RS20975: EX31_RS20975 - glutamate-5-semialdehyde dehydrogenase, at 837,961 to 839,214
_RS20975
EX31_RS20980: EX31_RS20980 - glutamate 5-kinase, at 839,225 to 840,328
_RS20980
Position (kb)
837
838
839
840 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 836.982 kb on + strand at 837.019 kb on + strand at 837.085 kb on - strand at 837.168 kb on + strand, within EX31_RS20970 at 837.168 kb on + strand, within EX31_RS20970 at 837.194 kb on + strand, within EX31_RS20970 at 837.194 kb on + strand, within EX31_RS20970 at 837.195 kb on - strand, within EX31_RS20970 at 837.195 kb on - strand, within EX31_RS20970 at 837.195 kb on - strand, within EX31_RS20970 at 837.195 kb on - strand, within EX31_RS20970 at 837.197 kb on + strand, within EX31_RS20970 at 837.197 kb on + strand, within EX31_RS20970 at 837.197 kb on + strand, within EX31_RS20970 at 837.198 kb on - strand, within EX31_RS20970 at 837.312 kb on - strand, within EX31_RS20970 at 837.448 kb on + strand, within EX31_RS20970 at 837.512 kb on + strand, within EX31_RS20970 at 837.553 kb on + strand, within EX31_RS20970 at 837.565 kb on + strand, within EX31_RS20970 at 837.566 kb on - strand, within EX31_RS20970 at 837.566 kb on - strand, within EX31_RS20970 at 837.623 kb on - strand, within EX31_RS20970 at 837.670 kb on + strand, within EX31_RS20970 at 837.702 kb on + strand, within EX31_RS20970 at 837.728 kb on + strand, within EX31_RS20970 at 837.814 kb on + strand at 837.814 kb on + strand at 837.815 kb on - strand at 837.815 kb on - strand at 837.863 kb on - strand at 837.963 kb on - strand at 837.974 kb on - strand at 838.001 kb on + strand at 838.001 kb on + strand at 838.001 kb on + strand at 838.001 kb on + strand at 838.001 kb on + strand at 838.002 kb on - strand at 838.018 kb on + strand at 838.122 kb on - strand, within EX31_RS20975 at 838.138 kb on + strand, within EX31_RS20975 at 838.138 kb on + strand, within EX31_RS20975 at 838.138 kb on + strand, within EX31_RS20975 at 838.139 kb on - strand, within EX31_RS20975 at 838.139 kb on - strand, within EX31_RS20975 at 838.139 kb on - strand, within EX31_RS20975 at 838.180 kb on - strand, within EX31_RS20975 at 838.208 kb on + strand, within EX31_RS20975 at 838.363 kb on + strand, within EX31_RS20975 at 838.368 kb on + strand, within EX31_RS20975 at 838.368 kb on + strand, within EX31_RS20975 at 838.456 kb on - strand, within EX31_RS20975 at 838.537 kb on - strand, within EX31_RS20975 at 838.637 kb on + strand, within EX31_RS20975 at 838.637 kb on + strand, within EX31_RS20975 at 838.637 kb on + strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 838.638 kb on - strand, within EX31_RS20975 at 839.088 kb on + strand, within EX31_RS20975 at 839.088 kb on + strand, within EX31_RS20975 at 839.089 kb on - strand at 839.089 kb on - strand at 839.089 kb on - strand at 839.208 kb on + strand at 839.226 kb on + strand at 839.227 kb on - strand at 839.227 kb on - strand at 839.275 kb on - strand at 839.338 kb on + strand, within EX31_RS20980 at 839.339 kb on - strand, within EX31_RS20980 at 839.339 kb on - strand, within EX31_RS20980 at 839.339 kb on - strand, within EX31_RS20980 at 839.340 kb on + strand, within EX31_RS20980 at 839.340 kb on + strand, within EX31_RS20980 at 839.341 kb on - strand, within EX31_RS20980 at 839.341 kb on - strand, within EX31_RS20980 at 839.632 kb on + strand, within EX31_RS20980 at 839.857 kb on + strand, within EX31_RS20980 at 839.944 kb on + strand, within EX31_RS20980 at 839.944 kb on + strand, within EX31_RS20980 at 840.048 kb on + strand, within EX31_RS20980 at 840.048 kb on + strand, within EX31_RS20980 at 840.048 kb on + strand, within EX31_RS20980 at 840.049 kb on - strand, within EX31_RS20980 at 840.049 kb on - strand, within EX31_RS20980 at 840.049 kb on - strand, within EX31_RS20980 at 840.049 kb on - strand, within EX31_RS20980 at 840.049 kb on - strand, within EX31_RS20980
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 836,982 + +1.2 837,019 + +0.4 837,085 - -0.0 837,168 + EX31_RS20970 0.19 -0.0 837,168 + EX31_RS20970 0.19 -0.4 837,194 + EX31_RS20970 0.21 +1.4 837,194 + EX31_RS20970 0.21 -0.3 837,195 - EX31_RS20970 0.22 -1.4 837,195 - EX31_RS20970 0.22 +0.6 837,195 - EX31_RS20970 0.22 -0.7 837,195 - EX31_RS20970 0.22 -0.0 837,197 + EX31_RS20970 0.22 -0.7 837,197 + EX31_RS20970 0.22 -0.0 837,197 + EX31_RS20970 0.22 -0.0 837,198 - EX31_RS20970 0.22 -1.5 837,312 - EX31_RS20970 0.35 +0.3 837,448 + EX31_RS20970 0.50 -0.2 837,512 + EX31_RS20970 0.57 +0.9 837,553 + EX31_RS20970 0.62 -0.4 837,565 + EX31_RS20970 0.63 +0.0 837,566 - EX31_RS20970 0.63 -0.7 837,566 - EX31_RS20970 0.63 -0.7 837,623 - EX31_RS20970 0.69 +0.1 837,670 + EX31_RS20970 0.75 -1.5 837,702 + EX31_RS20970 0.78 +0.2 837,728 + EX31_RS20970 0.81 +0.2 837,814 + +0.2 837,814 + +0.7 837,815 - +3.2 837,815 - -1.0 837,863 - +0.1 837,963 - -1.0 837,974 - -1.2 838,001 + -1.5 838,001 + -2.8 838,001 + -2.8 838,001 + -3.1 838,001 + -2.1 838,002 - -3.6 838,018 + -2.7 838,122 - EX31_RS20975 0.13 -2.9 838,138 + EX31_RS20975 0.14 -1.8 838,138 + EX31_RS20975 0.14 +0.1 838,138 + EX31_RS20975 0.14 -2.2 838,139 - EX31_RS20975 0.14 -2.7 838,139 - EX31_RS20975 0.14 -4.3 838,139 - EX31_RS20975 0.14 -3.4 838,180 - EX31_RS20975 0.17 -2.7 838,208 + EX31_RS20975 0.20 +0.1 838,363 + EX31_RS20975 0.32 -2.2 838,368 + EX31_RS20975 0.32 -2.1 838,368 + EX31_RS20975 0.32 -2.6 838,456 - EX31_RS20975 0.39 -3.9 838,537 - EX31_RS20975 0.46 -1.2 838,637 + EX31_RS20975 0.54 -2.4 838,637 + EX31_RS20975 0.54 -2.0 838,637 + EX31_RS20975 0.54 -3.1 838,638 - EX31_RS20975 0.54 -1.4 838,638 - EX31_RS20975 0.54 -2.0 838,638 - EX31_RS20975 0.54 -0.9 838,638 - EX31_RS20975 0.54 -0.7 838,638 - EX31_RS20975 0.54 -2.2 838,638 - EX31_RS20975 0.54 -4.2 838,638 - EX31_RS20975 0.54 -3.5 838,638 - EX31_RS20975 0.54 -2.2 839,088 + EX31_RS20975 0.90 -4.1 839,088 + EX31_RS20975 0.90 -3.2 839,089 - -2.6 839,089 - -2.0 839,089 - +0.1 839,208 + -2.0 839,226 + -0.9 839,227 - +0.8 839,227 - -0.4 839,275 - -1.9 839,338 + EX31_RS20980 0.10 -1.8 839,339 - EX31_RS20980 0.10 -4.1 839,339 - EX31_RS20980 0.10 -3.0 839,339 - EX31_RS20980 0.10 -3.9 839,340 + EX31_RS20980 0.10 -2.8 839,340 + EX31_RS20980 0.10 -1.2 839,341 - EX31_RS20980 0.11 -3.7 839,341 - EX31_RS20980 0.11 -4.0 839,632 + EX31_RS20980 0.37 -1.1 839,857 + EX31_RS20980 0.57 -0.2 839,944 + EX31_RS20980 0.65 -3.0 839,944 + EX31_RS20980 0.65 -0.7 840,048 + EX31_RS20980 0.75 -3.3 840,048 + EX31_RS20980 0.75 -1.7 840,048 + EX31_RS20980 0.75 -2.3 840,049 - EX31_RS20980 0.75 -3.0 840,049 - EX31_RS20980 0.75 -2.6 840,049 - EX31_RS20980 0.75 -1.9 840,049 - EX31_RS20980 0.75 -3.5 840,049 - EX31_RS20980 0.75 -4.6
Or see this region's nucleotide sequence