Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS20210 and EX31_RS20215 overlap by 11 nucleotides EX31_RS20215 and EX31_RS20220 overlap by 1 nucleotides EX31_RS20220 and EX31_RS20225 overlap by 1 nucleotides
EX31_RS20210: EX31_RS20210 - sulfate/thiosulfate ABC transporter ATP-binding protein CysA, at 670,572 to 671,666
_RS20210
EX31_RS20215: EX31_RS20215 - sulfate/thiosulfate ABC transporter permease CysW, at 671,656 to 672,531
_RS20215
EX31_RS20220: EX31_RS20220 - sulfate/thiosulfate ABC transporter permease CysT, at 672,531 to 673,361
_RS20220
EX31_RS20225: EX31_RS20225 - sulfate ABC transporter substrate-binding protein, at 673,361 to 674,383
_RS20225
Position (kb) 672
673
674 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 671.606 kb on + strand at 671.607 kb on - strand at 671.607 kb on - strand at 671.607 kb on - strand at 671.621 kb on + strand at 671.622 kb on - strand at 671.622 kb on - strand at 671.636 kb on - strand at 671.645 kb on + strand at 671.795 kb on + strand, within EX31_RS20215 at 671.795 kb on + strand, within EX31_RS20215 at 671.795 kb on + strand, within EX31_RS20215 at 671.796 kb on - strand, within EX31_RS20215 at 671.796 kb on - strand, within EX31_RS20215 at 671.810 kb on - strand, within EX31_RS20215 at 671.810 kb on - strand, within EX31_RS20215 at 671.821 kb on + strand, within EX31_RS20215 at 671.821 kb on + strand, within EX31_RS20215 at 671.821 kb on + strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.822 kb on - strand, within EX31_RS20215 at 671.834 kb on + strand, within EX31_RS20215 at 671.834 kb on + strand, within EX31_RS20215 at 671.835 kb on - strand, within EX31_RS20215 at 671.835 kb on - strand, within EX31_RS20215 at 671.850 kb on + strand, within EX31_RS20215 at 671.851 kb on - strand, within EX31_RS20215 at 671.851 kb on - strand, within EX31_RS20215 at 671.918 kb on + strand, within EX31_RS20215 at 671.918 kb on + strand, within EX31_RS20215 at 671.919 kb on - strand, within EX31_RS20215 at 671.919 kb on - strand, within EX31_RS20215 at 671.923 kb on + strand, within EX31_RS20215 at 671.923 kb on + strand, within EX31_RS20215 at 671.923 kb on + strand, within EX31_RS20215 at 671.987 kb on - strand, within EX31_RS20215 at 672.070 kb on + strand, within EX31_RS20215 at 672.071 kb on - strand, within EX31_RS20215 at 672.071 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.106 kb on - strand, within EX31_RS20215 at 672.158 kb on + strand, within EX31_RS20215 at 672.159 kb on - strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.170 kb on + strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.171 kb on - strand, within EX31_RS20215 at 672.227 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.230 kb on - strand, within EX31_RS20215 at 672.336 kb on + strand, within EX31_RS20215 at 672.337 kb on - strand, within EX31_RS20215 at 672.337 kb on - strand, within EX31_RS20215 at 672.441 kb on + strand, within EX31_RS20215 at 672.441 kb on + strand, within EX31_RS20215 at 672.489 kb on + strand at 672.490 kb on - strand at 672.490 kb on - strand at 672.533 kb on - strand at 672.568 kb on - strand at 672.654 kb on + strand, within EX31_RS20220 at 672.705 kb on - strand, within EX31_RS20220 at 672.857 kb on - strand, within EX31_RS20220 at 672.867 kb on + strand, within EX31_RS20220 at 672.918 kb on - strand, within EX31_RS20220 at 672.918 kb on - strand, within EX31_RS20220 at 672.918 kb on - strand, within EX31_RS20220 at 672.939 kb on - strand, within EX31_RS20220 at 672.952 kb on + strand, within EX31_RS20220 at 672.953 kb on - strand, within EX31_RS20220 at 673.005 kb on - strand, within EX31_RS20220 at 673.145 kb on + strand, within EX31_RS20220 at 673.179 kb on + strand, within EX31_RS20220 at 673.179 kb on + strand, within EX31_RS20220 at 673.179 kb on + strand, within EX31_RS20220 at 673.184 kb on + strand, within EX31_RS20220 at 673.187 kb on - strand, within EX31_RS20220 at 673.226 kb on - strand, within EX31_RS20220 at 673.339 kb on - strand at 673.362 kb on + strand at 673.362 kb on + strand at 673.362 kb on + strand at 673.362 kb on + strand at 673.362 kb on + strand at 673.362 kb on + strand at 673.362 kb on + strand at 673.363 kb on - strand at 673.363 kb on - strand at 673.363 kb on - strand at 673.363 kb on - strand at 673.363 kb on - strand at 673.363 kb on - strand at 673.363 kb on - strand at 673.363 kb on - strand at 673.380 kb on - strand at 673.380 kb on - strand at 673.515 kb on + strand, within EX31_RS20225 at 673.525 kb on - strand, within EX31_RS20225 at 673.528 kb on + strand, within EX31_RS20225 at 673.528 kb on + strand, within EX31_RS20225 at 673.570 kb on - strand, within EX31_RS20225 at 673.570 kb on - strand, within EX31_RS20225 at 673.680 kb on + strand, within EX31_RS20225 at 673.680 kb on + strand, within EX31_RS20225 at 673.681 kb on - strand, within EX31_RS20225 at 673.684 kb on + strand, within EX31_RS20225 at 673.684 kb on + strand, within EX31_RS20225 at 673.685 kb on - strand, within EX31_RS20225 at 673.702 kb on + strand, within EX31_RS20225 at 673.893 kb on + strand, within EX31_RS20225 at 673.893 kb on + strand, within EX31_RS20225 at 673.893 kb on + strand, within EX31_RS20225 at 673.894 kb on - strand, within EX31_RS20225 at 673.894 kb on - strand, within EX31_RS20225 at 673.894 kb on - strand, within EX31_RS20225 at 673.894 kb on - strand, within EX31_RS20225 at 673.894 kb on - strand, within EX31_RS20225 at 673.894 kb on - strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.897 kb on + strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.898 kb on - strand, within EX31_RS20225 at 673.900 kb on - strand, within EX31_RS20225 at 673.958 kb on + strand, within EX31_RS20225 at 673.959 kb on - strand, within EX31_RS20225 at 673.996 kb on + strand, within EX31_RS20225 at 673.996 kb on + strand, within EX31_RS20225 at 673.997 kb on - strand, within EX31_RS20225 at 674.000 kb on + strand, within EX31_RS20225 at 674.001 kb on - strand, within EX31_RS20225 at 674.001 kb on - strand, within EX31_RS20225 at 674.022 kb on + strand, within EX31_RS20225 at 674.023 kb on - strand, within EX31_RS20225 at 674.023 kb on - strand, within EX31_RS20225 at 674.038 kb on + strand, within EX31_RS20225 at 674.038 kb on + strand, within EX31_RS20225 at 674.038 kb on + strand, within EX31_RS20225 at 674.039 kb on - strand, within EX31_RS20225 at 674.039 kb on - strand, within EX31_RS20225 at 674.039 kb on - strand, within EX31_RS20225 at 674.041 kb on + strand, within EX31_RS20225 at 674.042 kb on - strand, within EX31_RS20225 at 674.116 kb on + strand, within EX31_RS20225 at 674.118 kb on + strand, within EX31_RS20225 at 674.118 kb on + strand, within EX31_RS20225 at 674.118 kb on + strand, within EX31_RS20225 at 674.118 kb on + strand, within EX31_RS20225 at 674.119 kb on - strand, within EX31_RS20225 at 674.119 kb on - strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.280 kb on + strand, within EX31_RS20225 at 674.281 kb on - strand at 674.281 kb on - strand at 674.281 kb on - strand at 674.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 671,606 + -1.5 671,607 - -0.7 671,607 - -1.2 671,607 - -0.9 671,621 + -0.7 671,622 - -0.4 671,622 - +0.2 671,636 - -1.6 671,645 + -1.6 671,795 + EX31_RS20215 0.16 -0.3 671,795 + EX31_RS20215 0.16 +0.2 671,795 + EX31_RS20215 0.16 -0.3 671,796 - EX31_RS20215 0.16 -0.4 671,796 - EX31_RS20215 0.16 -0.1 671,810 - EX31_RS20215 0.18 -2.2 671,810 - EX31_RS20215 0.18 +0.2 671,821 + EX31_RS20215 0.19 -2.4 671,821 + EX31_RS20215 0.19 +0.5 671,821 + EX31_RS20215 0.19 +0.4 671,822 - EX31_RS20215 0.19 -1.5 671,822 - EX31_RS20215 0.19 +1.1 671,822 - EX31_RS20215 0.19 +0.8 671,822 - EX31_RS20215 0.19 +0.3 671,822 - EX31_RS20215 0.19 +0.6 671,834 + EX31_RS20215 0.20 -0.4 671,834 + EX31_RS20215 0.20 -0.3 671,835 - EX31_RS20215 0.20 -0.3 671,835 - EX31_RS20215 0.20 -0.4 671,850 + EX31_RS20215 0.22 -2.0 671,851 - EX31_RS20215 0.22 +0.5 671,851 - EX31_RS20215 0.22 -1.6 671,918 + EX31_RS20215 0.30 -0.3 671,918 + EX31_RS20215 0.30 -1.6 671,919 - EX31_RS20215 0.30 -1.4 671,919 - EX31_RS20215 0.30 -0.8 671,923 + EX31_RS20215 0.30 +0.7 671,923 + EX31_RS20215 0.30 -1.4 671,923 + EX31_RS20215 0.30 +1.1 671,987 - EX31_RS20215 0.38 +0.0 672,070 + EX31_RS20215 0.47 -1.3 672,071 - EX31_RS20215 0.47 -0.0 672,071 - EX31_RS20215 0.47 -1.4 672,106 - EX31_RS20215 0.51 -0.6 672,106 - EX31_RS20215 0.51 -0.4 672,106 - EX31_RS20215 0.51 -0.0 672,106 - EX31_RS20215 0.51 -1.3 672,158 + EX31_RS20215 0.57 -0.3 672,159 - EX31_RS20215 0.57 -0.1 672,170 + EX31_RS20215 0.59 -1.6 672,170 + EX31_RS20215 0.59 -0.3 672,170 + EX31_RS20215 0.59 +1.6 672,170 + EX31_RS20215 0.59 -0.3 672,171 - EX31_RS20215 0.59 -0.4 672,171 - EX31_RS20215 0.59 +0.9 672,171 - EX31_RS20215 0.59 +1.1 672,171 - EX31_RS20215 0.59 -0.4 672,171 - EX31_RS20215 0.59 -0.5 672,171 - EX31_RS20215 0.59 -0.5 672,227 - EX31_RS20215 0.65 -0.7 672,230 - EX31_RS20215 0.66 -1.4 672,230 - EX31_RS20215 0.66 -0.7 672,230 - EX31_RS20215 0.66 -1.3 672,230 - EX31_RS20215 0.66 -1.5 672,336 + EX31_RS20215 0.78 -0.3 672,337 - EX31_RS20215 0.78 -1.5 672,337 - EX31_RS20215 0.78 +0.9 672,441 + EX31_RS20215 0.90 -1.6 672,441 + EX31_RS20215 0.90 -1.1 672,489 + -1.1 672,490 - +0.7 672,490 - -1.7 672,533 - -0.6 672,568 - -1.0 672,654 + EX31_RS20220 0.15 -1.9 672,705 - EX31_RS20220 0.21 -0.4 672,857 - EX31_RS20220 0.39 -0.4 672,867 + EX31_RS20220 0.40 -1.2 672,918 - EX31_RS20220 0.47 -0.7 672,918 - EX31_RS20220 0.47 +0.5 672,918 - EX31_RS20220 0.47 -2.0 672,939 - EX31_RS20220 0.49 -1.4 672,952 + EX31_RS20220 0.51 +0.3 672,953 - EX31_RS20220 0.51 +0.1 673,005 - EX31_RS20220 0.57 -0.6 673,145 + EX31_RS20220 0.74 -1.5 673,179 + EX31_RS20220 0.78 -2.1 673,179 + EX31_RS20220 0.78 +0.8 673,179 + EX31_RS20220 0.78 -0.3 673,184 + EX31_RS20220 0.79 -0.9 673,187 - EX31_RS20220 0.79 +0.1 673,226 - EX31_RS20220 0.84 -1.3 673,339 - -0.1 673,362 + +0.0 673,362 + +0.3 673,362 + +1.1 673,362 + +0.1 673,362 + -1.9 673,362 + -0.4 673,362 + -1.1 673,363 - -0.5 673,363 - +1.4 673,363 - -0.1 673,363 - -0.1 673,363 - -0.1 673,363 - -1.1 673,363 - -0.4 673,363 - -0.5 673,380 - -1.2 673,380 - -1.3 673,515 + EX31_RS20225 0.15 -0.5 673,525 - EX31_RS20225 0.16 +1.2 673,528 + EX31_RS20225 0.16 +0.1 673,528 + EX31_RS20225 0.16 -0.6 673,570 - EX31_RS20225 0.20 -0.0 673,570 - EX31_RS20225 0.20 -1.0 673,680 + EX31_RS20225 0.31 +0.4 673,680 + EX31_RS20225 0.31 +0.5 673,681 - EX31_RS20225 0.31 -0.7 673,684 + EX31_RS20225 0.32 +0.4 673,684 + EX31_RS20225 0.32 -0.8 673,685 - EX31_RS20225 0.32 -0.2 673,702 + EX31_RS20225 0.33 +0.6 673,893 + EX31_RS20225 0.52 -1.7 673,893 + EX31_RS20225 0.52 -1.3 673,893 + EX31_RS20225 0.52 -0.6 673,894 - EX31_RS20225 0.52 +2.1 673,894 - EX31_RS20225 0.52 +0.4 673,894 - EX31_RS20225 0.52 +0.1 673,894 - EX31_RS20225 0.52 -1.4 673,894 - EX31_RS20225 0.52 -0.5 673,894 - EX31_RS20225 0.52 -0.9 673,897 + EX31_RS20225 0.52 +0.2 673,897 + EX31_RS20225 0.52 -1.3 673,897 + EX31_RS20225 0.52 -1.5 673,897 + EX31_RS20225 0.52 -1.0 673,897 + EX31_RS20225 0.52 +0.3 673,897 + EX31_RS20225 0.52 -0.3 673,897 + EX31_RS20225 0.52 -0.7 673,897 + EX31_RS20225 0.52 -0.2 673,897 + EX31_RS20225 0.52 -0.9 673,897 + EX31_RS20225 0.52 +2.1 673,897 + EX31_RS20225 0.52 +0.1 673,897 + EX31_RS20225 0.52 -3.6 673,898 - EX31_RS20225 0.52 +0.6 673,898 - EX31_RS20225 0.52 -0.7 673,898 - EX31_RS20225 0.52 +0.4 673,898 - EX31_RS20225 0.52 +0.3 673,898 - EX31_RS20225 0.52 +0.5 673,898 - EX31_RS20225 0.52 +0.9 673,898 - EX31_RS20225 0.52 +1.3 673,898 - EX31_RS20225 0.52 -1.2 673,898 - EX31_RS20225 0.52 -0.1 673,898 - EX31_RS20225 0.52 -0.6 673,898 - EX31_RS20225 0.52 -0.1 673,898 - EX31_RS20225 0.52 -1.9 673,898 - EX31_RS20225 0.52 +1.1 673,898 - EX31_RS20225 0.52 +0.7 673,898 - EX31_RS20225 0.52 -0.3 673,898 - EX31_RS20225 0.52 -0.2 673,898 - EX31_RS20225 0.52 -0.6 673,900 - EX31_RS20225 0.53 -0.3 673,958 + EX31_RS20225 0.58 -1.5 673,959 - EX31_RS20225 0.58 +0.1 673,996 + EX31_RS20225 0.62 -0.3 673,996 + EX31_RS20225 0.62 +0.8 673,997 - EX31_RS20225 0.62 +2.1 674,000 + EX31_RS20225 0.62 -1.1 674,001 - EX31_RS20225 0.63 +0.9 674,001 - EX31_RS20225 0.63 -0.1 674,022 + EX31_RS20225 0.65 -1.1 674,023 - EX31_RS20225 0.65 -0.3 674,023 - EX31_RS20225 0.65 -0.7 674,038 + EX31_RS20225 0.66 -0.8 674,038 + EX31_RS20225 0.66 +1.3 674,038 + EX31_RS20225 0.66 -0.4 674,039 - EX31_RS20225 0.66 +0.3 674,039 - EX31_RS20225 0.66 -0.6 674,039 - EX31_RS20225 0.66 -0.4 674,041 + EX31_RS20225 0.66 -0.9 674,042 - EX31_RS20225 0.67 +0.5 674,116 + EX31_RS20225 0.74 +0.7 674,118 + EX31_RS20225 0.74 +0.1 674,118 + EX31_RS20225 0.74 +0.8 674,118 + EX31_RS20225 0.74 -0.7 674,118 + EX31_RS20225 0.74 -1.3 674,119 - EX31_RS20225 0.74 -1.2 674,119 - EX31_RS20225 0.74 -0.2 674,280 + EX31_RS20225 0.90 -0.3 674,280 + EX31_RS20225 0.90 +0.4 674,280 + EX31_RS20225 0.90 -0.3 674,280 + EX31_RS20225 0.90 +0.1 674,280 + EX31_RS20225 0.90 +0.1 674,280 + EX31_RS20225 0.90 -0.5 674,280 + EX31_RS20225 0.90 -0.2 674,280 + EX31_RS20225 0.90 -1.0 674,280 + EX31_RS20225 0.90 -2.5 674,280 + EX31_RS20225 0.90 -0.8 674,280 + EX31_RS20225 0.90 -1.3 674,280 + EX31_RS20225 0.90 -1.1 674,280 + EX31_RS20225 0.90 +1.1 674,281 - -2.4 674,281 - -1.8 674,281 - -0.4 674,281 - -1.1
Or see this region's nucleotide sequence