Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18580 and EX31_RS18585 are separated by 189 nucleotides EX31_RS18585 and EX31_RS18590 are separated by 166 nucleotides
EX31_RS18580: EX31_RS18580 - ATP-dependent endonuclease, at 292,377 to 294,110
_RS18580
EX31_RS18585: EX31_RS18585 - lysine exporter LysO family protein, at 294,300 to 295,199
_RS18585
EX31_RS18590: EX31_RS18590 - hydroxylamine reductase, at 295,366 to 297,018
_RS18590
Position (kb) 294
295
296 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 293.384 kb on - strand, within EX31_RS18580 at 293.504 kb on + strand, within EX31_RS18580 at 293.504 kb on + strand, within EX31_RS18580 at 293.574 kb on + strand, within EX31_RS18580 at 293.574 kb on + strand, within EX31_RS18580 at 293.575 kb on - strand, within EX31_RS18580 at 293.575 kb on - strand, within EX31_RS18580 at 293.575 kb on - strand, within EX31_RS18580 at 293.575 kb on - strand, within EX31_RS18580 at 293.575 kb on - strand, within EX31_RS18580 at 293.631 kb on + strand, within EX31_RS18580 at 293.632 kb on - strand, within EX31_RS18580 at 293.710 kb on + strand, within EX31_RS18580 at 293.711 kb on - strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.713 kb on + strand, within EX31_RS18580 at 293.714 kb on - strand, within EX31_RS18580 at 293.714 kb on - strand, within EX31_RS18580 at 293.714 kb on - strand, within EX31_RS18580 at 293.714 kb on - strand, within EX31_RS18580 at 293.714 kb on - strand, within EX31_RS18580 at 293.714 kb on - strand, within EX31_RS18580 at 293.717 kb on + strand, within EX31_RS18580 at 293.717 kb on + strand, within EX31_RS18580 at 293.717 kb on + strand, within EX31_RS18580 at 293.717 kb on + strand, within EX31_RS18580 at 293.717 kb on + strand, within EX31_RS18580 at 293.780 kb on + strand, within EX31_RS18580 at 293.780 kb on + strand, within EX31_RS18580 at 293.780 kb on + strand, within EX31_RS18580 at 293.780 kb on + strand, within EX31_RS18580 at 293.780 kb on + strand, within EX31_RS18580 at 293.780 kb on + strand, within EX31_RS18580 at 293.780 kb on + strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.781 kb on - strand, within EX31_RS18580 at 293.811 kb on - strand, within EX31_RS18580 at 293.827 kb on + strand, within EX31_RS18580 at 293.827 kb on + strand, within EX31_RS18580 at 293.827 kb on + strand, within EX31_RS18580 at 293.863 kb on + strand, within EX31_RS18580 at 293.864 kb on - strand, within EX31_RS18580 at 293.864 kb on - strand, within EX31_RS18580 at 293.879 kb on + strand, within EX31_RS18580 at 293.887 kb on + strand, within EX31_RS18580 at 293.887 kb on + strand, within EX31_RS18580 at 293.888 kb on - strand, within EX31_RS18580 at 293.888 kb on - strand, within EX31_RS18580 at 293.888 kb on - strand, within EX31_RS18580 at 293.888 kb on - strand, within EX31_RS18580 at 293.895 kb on - strand, within EX31_RS18580 at 293.919 kb on + strand, within EX31_RS18580 at 293.919 kb on + strand, within EX31_RS18580 at 293.919 kb on + strand, within EX31_RS18580 at 293.920 kb on - strand, within EX31_RS18580 at 293.965 kb on - strand at 294.089 kb on + strand at 294.089 kb on + strand at 294.089 kb on + strand at 294.090 kb on - strand at 294.090 kb on - strand at 294.106 kb on + strand at 294.106 kb on + strand at 294.107 kb on - strand at 294.184 kb on + strand at 294.184 kb on + strand at 294.184 kb on + strand at 294.185 kb on - strand at 294.185 kb on - strand at 294.208 kb on + strand at 294.209 kb on - strand at 294.249 kb on + strand at 294.249 kb on + strand at 294.249 kb on + strand at 294.249 kb on + strand at 294.250 kb on - strand at 294.250 kb on - strand at 294.250 kb on - strand at 294.250 kb on - strand at 294.250 kb on - strand at 294.250 kb on - strand at 294.277 kb on + strand at 294.278 kb on - strand at 294.278 kb on - strand at 294.303 kb on + strand at 294.303 kb on + strand at 294.303 kb on + strand at 294.303 kb on + strand at 294.304 kb on - strand at 294.304 kb on - strand at 294.316 kb on + strand at 294.317 kb on - strand at 294.320 kb on + strand at 294.320 kb on + strand at 294.321 kb on - strand at 294.321 kb on - strand at 294.321 kb on - strand at 294.350 kb on + strand at 294.350 kb on + strand at 294.351 kb on - strand at 294.394 kb on + strand, within EX31_RS18585 at 294.395 kb on - strand, within EX31_RS18585 at 294.411 kb on + strand, within EX31_RS18585 at 294.411 kb on + strand, within EX31_RS18585 at 294.411 kb on + strand, within EX31_RS18585 at 294.411 kb on + strand, within EX31_RS18585 at 294.412 kb on - strand, within EX31_RS18585 at 294.412 kb on - strand, within EX31_RS18585 at 294.412 kb on - strand, within EX31_RS18585 at 294.412 kb on - strand, within EX31_RS18585 at 294.412 kb on - strand, within EX31_RS18585 at 294.412 kb on - strand, within EX31_RS18585 at 294.489 kb on + strand, within EX31_RS18585 at 294.489 kb on + strand, within EX31_RS18585 at 294.490 kb on - strand, within EX31_RS18585 at 294.528 kb on + strand, within EX31_RS18585 at 294.528 kb on + strand, within EX31_RS18585 at 294.576 kb on + strand, within EX31_RS18585 at 294.577 kb on - strand, within EX31_RS18585 at 294.578 kb on + strand, within EX31_RS18585 at 294.579 kb on - strand, within EX31_RS18585 at 294.687 kb on - strand, within EX31_RS18585 at 294.721 kb on + strand, within EX31_RS18585 at 294.721 kb on + strand, within EX31_RS18585 at 294.721 kb on + strand, within EX31_RS18585 at 294.721 kb on + strand, within EX31_RS18585 at 294.915 kb on + strand, within EX31_RS18585 at 294.915 kb on + strand, within EX31_RS18585 at 294.916 kb on - strand, within EX31_RS18585 at 294.980 kb on + strand, within EX31_RS18585 at 295.086 kb on - strand, within EX31_RS18585 at 295.122 kb on - strand at 295.122 kb on - strand at 295.151 kb on + strand at 295.177 kb on + strand at 295.178 kb on - strand at 295.256 kb on - strand at 295.256 kb on - strand at 295.366 kb on - strand at 295.366 kb on - strand at 295.366 kb on - strand at 295.424 kb on - strand at 295.434 kb on + strand at 295.434 kb on + strand at 295.434 kb on + strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.435 kb on - strand at 295.476 kb on - strand at 295.476 kb on - strand at 295.490 kb on + strand at 295.595 kb on + strand, within EX31_RS18590 at 295.596 kb on - strand, within EX31_RS18590 at 295.596 kb on - strand, within EX31_RS18590 at 295.596 kb on - strand, within EX31_RS18590 at 295.821 kb on + strand, within EX31_RS18590 at 295.821 kb on + strand, within EX31_RS18590 at 295.822 kb on - strand, within EX31_RS18590 at 295.822 kb on - strand, within EX31_RS18590 at 295.822 kb on - strand, within EX31_RS18590 at 295.835 kb on - strand, within EX31_RS18590 at 295.835 kb on - strand, within EX31_RS18590 at 295.858 kb on + strand, within EX31_RS18590 at 295.858 kb on + strand, within EX31_RS18590 at 295.858 kb on + strand, within EX31_RS18590 at 295.859 kb on - strand, within EX31_RS18590 at 295.859 kb on - strand, within EX31_RS18590 at 295.860 kb on + strand, within EX31_RS18590 at 295.903 kb on + strand, within EX31_RS18590 at 295.912 kb on + strand, within EX31_RS18590 at 295.913 kb on - strand, within EX31_RS18590 at 295.913 kb on - strand, within EX31_RS18590 at 296.088 kb on + strand, within EX31_RS18590 at 296.167 kb on - strand, within EX31_RS18590 at 296.167 kb on - strand, within EX31_RS18590 at 296.167 kb on - strand, within EX31_RS18590 at 296.170 kb on + strand, within EX31_RS18590 at 296.170 kb on + strand, within EX31_RS18590 at 296.170 kb on + strand, within EX31_RS18590 at 296.171 kb on - strand, within EX31_RS18590 at 296.171 kb on - strand, within EX31_RS18590
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 293,384 - EX31_RS18580 0.58 +0.8 293,504 + EX31_RS18580 0.65 -1.6 293,504 + EX31_RS18580 0.65 +1.1 293,574 + EX31_RS18580 0.69 +0.4 293,574 + EX31_RS18580 0.69 +0.1 293,575 - EX31_RS18580 0.69 -0.1 293,575 - EX31_RS18580 0.69 +1.3 293,575 - EX31_RS18580 0.69 -2.6 293,575 - EX31_RS18580 0.69 -1.8 293,575 - EX31_RS18580 0.69 +1.9 293,631 + EX31_RS18580 0.72 +1.5 293,632 - EX31_RS18580 0.72 +0.4 293,710 + EX31_RS18580 0.77 -0.9 293,711 - EX31_RS18580 0.77 +1.1 293,713 + EX31_RS18580 0.77 +1.4 293,713 + EX31_RS18580 0.77 +1.1 293,713 + EX31_RS18580 0.77 +0.4 293,713 + EX31_RS18580 0.77 +0.0 293,713 + EX31_RS18580 0.77 +0.7 293,713 + EX31_RS18580 0.77 -0.2 293,713 + EX31_RS18580 0.77 +0.8 293,713 + EX31_RS18580 0.77 -0.6 293,713 + EX31_RS18580 0.77 -1.1 293,713 + EX31_RS18580 0.77 +0.4 293,713 + EX31_RS18580 0.77 +2.7 293,713 + EX31_RS18580 0.77 +0.1 293,714 - EX31_RS18580 0.77 +0.5 293,714 - EX31_RS18580 0.77 -1.5 293,714 - EX31_RS18580 0.77 -1.1 293,714 - EX31_RS18580 0.77 +0.2 293,714 - EX31_RS18580 0.77 +0.8 293,714 - EX31_RS18580 0.77 +1.9 293,717 + EX31_RS18580 0.77 +1.4 293,717 + EX31_RS18580 0.77 -2.0 293,717 + EX31_RS18580 0.77 -1.1 293,717 + EX31_RS18580 0.77 +1.7 293,717 + EX31_RS18580 0.77 +1.1 293,780 + EX31_RS18580 0.81 +1.4 293,780 + EX31_RS18580 0.81 +0.3 293,780 + EX31_RS18580 0.81 -0.5 293,780 + EX31_RS18580 0.81 -0.9 293,780 + EX31_RS18580 0.81 +1.6 293,780 + EX31_RS18580 0.81 +0.3 293,780 + EX31_RS18580 0.81 -0.1 293,781 - EX31_RS18580 0.81 -0.1 293,781 - EX31_RS18580 0.81 +0.8 293,781 - EX31_RS18580 0.81 -1.4 293,781 - EX31_RS18580 0.81 -0.7 293,781 - EX31_RS18580 0.81 +0.4 293,781 - EX31_RS18580 0.81 +1.9 293,781 - EX31_RS18580 0.81 +0.9 293,781 - EX31_RS18580 0.81 -0.5 293,781 - EX31_RS18580 0.81 +0.1 293,781 - EX31_RS18580 0.81 +0.4 293,781 - EX31_RS18580 0.81 -0.6 293,811 - EX31_RS18580 0.83 +1.9 293,827 + EX31_RS18580 0.84 +0.8 293,827 + EX31_RS18580 0.84 +1.5 293,827 + EX31_RS18580 0.84 +0.6 293,863 + EX31_RS18580 0.86 +0.4 293,864 - EX31_RS18580 0.86 +1.4 293,864 - EX31_RS18580 0.86 -0.6 293,879 + EX31_RS18580 0.87 -1.1 293,887 + EX31_RS18580 0.87 +1.2 293,887 + EX31_RS18580 0.87 +1.6 293,888 - EX31_RS18580 0.87 -2.0 293,888 - EX31_RS18580 0.87 -0.2 293,888 - EX31_RS18580 0.87 +2.2 293,888 - EX31_RS18580 0.87 -1.2 293,895 - EX31_RS18580 0.88 +0.8 293,919 + EX31_RS18580 0.89 -0.9 293,919 + EX31_RS18580 0.89 -0.1 293,919 + EX31_RS18580 0.89 -0.1 293,920 - EX31_RS18580 0.89 +0.8 293,965 - -0.4 294,089 + +1.7 294,089 + -1.0 294,089 + +0.9 294,090 - -0.6 294,090 - +0.2 294,106 + -1.7 294,106 + +0.1 294,107 - -0.2 294,184 + -0.2 294,184 + +0.2 294,184 + -0.4 294,185 - +1.3 294,185 - +1.2 294,208 + +1.7 294,209 - +1.0 294,249 + +0.6 294,249 + -0.3 294,249 + -0.5 294,249 + -0.2 294,250 - -1.0 294,250 - +0.1 294,250 - -2.7 294,250 - +0.7 294,250 - +1.6 294,250 - -0.2 294,277 + -0.1 294,278 - +0.3 294,278 - -1.1 294,303 + +0.0 294,303 + -0.3 294,303 + +2.2 294,303 + +0.8 294,304 - +0.6 294,304 - +1.4 294,316 + -0.6 294,317 - -0.6 294,320 + -0.3 294,320 + -1.1 294,321 - +1.1 294,321 - -0.4 294,321 - -1.2 294,350 + -0.9 294,350 + +1.6 294,351 - -0.7 294,394 + EX31_RS18585 0.10 -1.9 294,395 - EX31_RS18585 0.11 -0.3 294,411 + EX31_RS18585 0.12 +0.8 294,411 + EX31_RS18585 0.12 -1.6 294,411 + EX31_RS18585 0.12 -2.0 294,411 + EX31_RS18585 0.12 +0.1 294,412 - EX31_RS18585 0.12 -0.5 294,412 - EX31_RS18585 0.12 +1.1 294,412 - EX31_RS18585 0.12 -0.1 294,412 - EX31_RS18585 0.12 -0.4 294,412 - EX31_RS18585 0.12 -0.7 294,412 - EX31_RS18585 0.12 -1.1 294,489 + EX31_RS18585 0.21 +1.1 294,489 + EX31_RS18585 0.21 -0.6 294,490 - EX31_RS18585 0.21 -0.1 294,528 + EX31_RS18585 0.25 +0.7 294,528 + EX31_RS18585 0.25 -0.4 294,576 + EX31_RS18585 0.31 +0.2 294,577 - EX31_RS18585 0.31 -0.6 294,578 + EX31_RS18585 0.31 -0.3 294,579 - EX31_RS18585 0.31 -0.6 294,687 - EX31_RS18585 0.43 -1.6 294,721 + EX31_RS18585 0.47 -0.7 294,721 + EX31_RS18585 0.47 -1.5 294,721 + EX31_RS18585 0.47 +0.1 294,721 + EX31_RS18585 0.47 +0.6 294,915 + EX31_RS18585 0.68 +0.1 294,915 + EX31_RS18585 0.68 +0.7 294,916 - EX31_RS18585 0.68 -0.0 294,980 + EX31_RS18585 0.76 +0.3 295,086 - EX31_RS18585 0.87 -0.9 295,122 - +1.8 295,122 - -1.9 295,151 + +0.9 295,177 + -0.1 295,178 - -1.1 295,256 - -1.0 295,256 - +1.3 295,366 - -0.8 295,366 - -0.3 295,366 - -0.4 295,424 - -1.1 295,434 + +0.2 295,434 + -0.9 295,434 + -0.1 295,435 - +1.1 295,435 - -0.8 295,435 - +0.2 295,435 - -0.2 295,435 - +0.6 295,435 - +0.1 295,435 - -0.1 295,435 - +0.0 295,435 - +0.6 295,476 - -0.5 295,476 - -0.3 295,490 + +0.5 295,595 + EX31_RS18590 0.14 -1.6 295,596 - EX31_RS18590 0.14 -0.5 295,596 - EX31_RS18590 0.14 +2.9 295,596 - EX31_RS18590 0.14 +0.9 295,821 + EX31_RS18590 0.28 -0.2 295,821 + EX31_RS18590 0.28 -0.9 295,822 - EX31_RS18590 0.28 +2.1 295,822 - EX31_RS18590 0.28 -1.6 295,822 - EX31_RS18590 0.28 +0.9 295,835 - EX31_RS18590 0.28 -0.8 295,835 - EX31_RS18590 0.28 +0.9 295,858 + EX31_RS18590 0.30 +0.9 295,858 + EX31_RS18590 0.30 +0.8 295,858 + EX31_RS18590 0.30 +0.2 295,859 - EX31_RS18590 0.30 -0.8 295,859 - EX31_RS18590 0.30 -2.3 295,860 + EX31_RS18590 0.30 -0.1 295,903 + EX31_RS18590 0.32 -0.1 295,912 + EX31_RS18590 0.33 -0.3 295,913 - EX31_RS18590 0.33 +0.7 295,913 - EX31_RS18590 0.33 +1.0 296,088 + EX31_RS18590 0.44 -0.6 296,167 - EX31_RS18590 0.48 -0.1 296,167 - EX31_RS18590 0.48 +0.7 296,167 - EX31_RS18590 0.48 -0.0 296,170 + EX31_RS18590 0.49 -1.8 296,170 + EX31_RS18590 0.49 +1.5 296,170 + EX31_RS18590 0.49 +1.9 296,171 - EX31_RS18590 0.49 +0.8 296,171 - EX31_RS18590 0.49 +0.9
Or see this region's nucleotide sequence