Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS12320 and EX31_RS12325 are separated by 147 nucleotides EX31_RS12325 and EX31_RS12330 are separated by 760 nucleotides
EX31_RS12320: EX31_RS12320 - metalloprotease PmbA, at 135,072 to 136,412
_RS12320
EX31_RS12325: EX31_RS12325 - cytochrome b562, at 136,560 to 136,946
_RS12325
EX31_RS12330: EX31_RS12330 - nucleoside diphosphate kinase regulator, at 137,707 to 138,117
_RS12330
Position (kb) 136
137 Strain fitness (log2 ratio) -2
-1
0
1
2 at 135.603 kb on + strand, within EX31_RS12320 at 135.603 kb on + strand, within EX31_RS12320 at 135.603 kb on + strand, within EX31_RS12320 at 135.604 kb on - strand, within EX31_RS12320 at 135.604 kb on - strand, within EX31_RS12320 at 135.604 kb on - strand, within EX31_RS12320 at 135.672 kb on + strand, within EX31_RS12320 at 135.672 kb on + strand, within EX31_RS12320 at 135.678 kb on + strand, within EX31_RS12320 at 135.692 kb on + strand, within EX31_RS12320 at 135.751 kb on + strand, within EX31_RS12320 at 135.867 kb on + strand, within EX31_RS12320 at 135.867 kb on + strand, within EX31_RS12320 at 135.868 kb on - strand, within EX31_RS12320 at 135.872 kb on + strand, within EX31_RS12320 at 136.053 kb on + strand, within EX31_RS12320 at 136.054 kb on - strand, within EX31_RS12320 at 136.054 kb on - strand, within EX31_RS12320 at 136.065 kb on - strand, within EX31_RS12320 at 136.154 kb on + strand, within EX31_RS12320 at 136.155 kb on - strand, within EX31_RS12320 at 136.221 kb on + strand, within EX31_RS12320 at 136.222 kb on - strand, within EX31_RS12320 at 136.222 kb on - strand, within EX31_RS12320 at 136.222 kb on - strand, within EX31_RS12320 at 136.222 kb on - strand, within EX31_RS12320 at 136.322 kb on + strand at 136.411 kb on - strand at 136.420 kb on + strand at 136.421 kb on - strand at 136.448 kb on + strand at 136.499 kb on - strand at 136.508 kb on + strand at 136.508 kb on + strand at 136.508 kb on + strand at 136.508 kb on + strand at 136.509 kb on - strand at 136.509 kb on - strand at 136.525 kb on + strand at 136.529 kb on + strand at 136.529 kb on + strand at 136.529 kb on + strand at 136.529 kb on + strand at 136.529 kb on + strand at 136.529 kb on + strand at 136.530 kb on - strand at 136.530 kb on - strand at 136.540 kb on + strand at 136.541 kb on - strand at 136.541 kb on - strand at 136.546 kb on + strand at 136.546 kb on + strand at 136.547 kb on - strand at 136.554 kb on + strand at 136.554 kb on + strand at 136.554 kb on + strand at 136.554 kb on + strand at 136.554 kb on + strand at 136.554 kb on + strand at 136.554 kb on + strand at 136.554 kb on + strand at 136.555 kb on - strand at 136.555 kb on - strand at 136.555 kb on - strand at 136.555 kb on - strand at 136.555 kb on - strand at 136.555 kb on - strand at 136.555 kb on - strand at 136.565 kb on + strand at 136.566 kb on - strand at 136.649 kb on + strand, within EX31_RS12325 at 136.650 kb on - strand, within EX31_RS12325 at 136.662 kb on - strand, within EX31_RS12325 at 136.665 kb on + strand, within EX31_RS12325 at 136.666 kb on - strand, within EX31_RS12325 at 136.666 kb on - strand, within EX31_RS12325 at 136.824 kb on - strand, within EX31_RS12325 at 136.907 kb on + strand, within EX31_RS12325 at 136.908 kb on - strand at 136.908 kb on - strand at 136.913 kb on + strand at 136.931 kb on + strand at 136.932 kb on - strand at 136.932 kb on - strand at 136.941 kb on - strand at 136.941 kb on - strand at 136.941 kb on - strand at 136.944 kb on + strand at 136.944 kb on + strand at 136.944 kb on + strand at 136.944 kb on + strand at 136.944 kb on + strand at 136.944 kb on + strand at 136.945 kb on - strand at 136.945 kb on - strand at 137.067 kb on - strand at 137.098 kb on + strand at 137.146 kb on + strand at 137.146 kb on + strand at 137.146 kb on + strand at 137.146 kb on + strand at 137.147 kb on - strand at 137.147 kb on - strand at 137.147 kb on - strand at 137.147 kb on - strand at 137.147 kb on - strand at 137.147 kb on - strand at 137.256 kb on - strand at 137.262 kb on + strand at 137.262 kb on + strand at 137.263 kb on - strand at 137.263 kb on - strand at 137.285 kb on + strand at 137.285 kb on + strand at 137.338 kb on + strand at 137.338 kb on + strand at 137.338 kb on + strand at 137.338 kb on + strand at 137.338 kb on + strand at 137.338 kb on + strand at 137.338 kb on + strand at 137.338 kb on + strand at 137.409 kb on + strand at 137.463 kb on + strand at 137.606 kb on + strand at 137.607 kb on - strand at 137.607 kb on - strand at 137.607 kb on - strand at 137.607 kb on - strand at 137.610 kb on + strand at 137.610 kb on + strand at 137.611 kb on - strand at 137.611 kb on - strand at 137.611 kb on - strand at 137.611 kb on - strand at 137.614 kb on - strand at 137.637 kb on + strand at 137.638 kb on - strand at 137.638 kb on - strand at 137.638 kb on - strand at 137.638 kb on - strand at 137.741 kb on + strand at 137.742 kb on - strand at 137.742 kb on - strand at 137.742 kb on - strand at 137.799 kb on + strand, within EX31_RS12330 at 137.800 kb on - strand, within EX31_RS12330 at 137.800 kb on - strand, within EX31_RS12330 at 137.800 kb on - strand, within EX31_RS12330 at 137.866 kb on - strand, within EX31_RS12330 at 137.866 kb on - strand, within EX31_RS12330 at 137.889 kb on - strand, within EX31_RS12330 at 137.890 kb on + strand, within EX31_RS12330 at 137.890 kb on + strand, within EX31_RS12330 at 137.890 kb on + strand, within EX31_RS12330 at 137.890 kb on + strand, within EX31_RS12330 at 137.890 kb on + strand, within EX31_RS12330 at 137.890 kb on + strand, within EX31_RS12330 at 137.890 kb on + strand, within EX31_RS12330 at 137.891 kb on - strand, within EX31_RS12330 at 137.891 kb on - strand, within EX31_RS12330 at 137.891 kb on - strand, within EX31_RS12330 at 137.922 kb on + strand, within EX31_RS12330 at 137.931 kb on + strand, within EX31_RS12330
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 135,603 + EX31_RS12320 0.40 +0.1 135,603 + EX31_RS12320 0.40 -1.1 135,603 + EX31_RS12320 0.40 -0.4 135,604 - EX31_RS12320 0.40 -0.1 135,604 - EX31_RS12320 0.40 +0.0 135,604 - EX31_RS12320 0.40 -0.8 135,672 + EX31_RS12320 0.45 +0.2 135,672 + EX31_RS12320 0.45 -0.4 135,678 + EX31_RS12320 0.45 +0.6 135,692 + EX31_RS12320 0.46 -1.4 135,751 + EX31_RS12320 0.51 -0.0 135,867 + EX31_RS12320 0.59 +0.1 135,867 + EX31_RS12320 0.59 -0.1 135,868 - EX31_RS12320 0.59 +0.2 135,872 + EX31_RS12320 0.60 +0.9 136,053 + EX31_RS12320 0.73 +0.2 136,054 - EX31_RS12320 0.73 +0.9 136,054 - EX31_RS12320 0.73 -1.5 136,065 - EX31_RS12320 0.74 -0.1 136,154 + EX31_RS12320 0.81 +0.0 136,155 - EX31_RS12320 0.81 -1.5 136,221 + EX31_RS12320 0.86 -0.0 136,222 - EX31_RS12320 0.86 -0.3 136,222 - EX31_RS12320 0.86 -0.1 136,222 - EX31_RS12320 0.86 -0.5 136,222 - EX31_RS12320 0.86 -0.8 136,322 + -0.1 136,411 - +1.4 136,420 + +0.7 136,421 - +1.0 136,448 + -1.9 136,499 - +0.3 136,508 + -0.1 136,508 + +0.1 136,508 + -0.3 136,508 + +0.2 136,509 - -0.8 136,509 - +0.7 136,525 + -0.2 136,529 + +1.4 136,529 + +0.0 136,529 + +0.7 136,529 + +0.6 136,529 + +0.4 136,529 + -0.5 136,530 - +0.1 136,530 - +0.5 136,540 + -0.2 136,541 - -0.9 136,541 - +0.1 136,546 + -1.4 136,546 + +0.5 136,547 - +0.3 136,554 + -0.1 136,554 + -0.0 136,554 + -0.2 136,554 + -0.1 136,554 + +1.2 136,554 + +0.9 136,554 + +0.0 136,554 + +0.2 136,555 - -0.8 136,555 - -1.0 136,555 - -1.3 136,555 - -0.6 136,555 - +0.8 136,555 - -0.2 136,555 - +1.9 136,565 + +0.3 136,566 - +0.5 136,649 + EX31_RS12325 0.23 +0.8 136,650 - EX31_RS12325 0.23 +0.5 136,662 - EX31_RS12325 0.26 -1.3 136,665 + EX31_RS12325 0.27 +0.8 136,666 - EX31_RS12325 0.27 +0.4 136,666 - EX31_RS12325 0.27 +0.2 136,824 - EX31_RS12325 0.68 -0.4 136,907 + EX31_RS12325 0.90 -0.2 136,908 - +1.0 136,908 - +0.4 136,913 + +1.6 136,931 + -0.1 136,932 - -0.6 136,932 - +0.3 136,941 - +0.2 136,941 - -0.4 136,941 - +0.9 136,944 + +0.7 136,944 + +0.9 136,944 + +0.4 136,944 + +0.6 136,944 + +0.1 136,944 + -0.8 136,945 - +1.0 136,945 - +0.9 137,067 - +0.7 137,098 + -2.6 137,146 + -0.9 137,146 + -0.9 137,146 + -0.2 137,146 + +0.4 137,147 - +1.0 137,147 - +0.8 137,147 - +0.0 137,147 - -0.8 137,147 - -0.1 137,147 - +0.2 137,256 - -0.4 137,262 + -0.8 137,262 + -0.3 137,263 - +0.6 137,263 - +0.5 137,285 + -0.2 137,285 + -0.4 137,338 + +0.0 137,338 + -1.3 137,338 + -0.9 137,338 + +0.0 137,338 + -0.2 137,338 + +0.5 137,338 + +0.5 137,338 + +2.2 137,409 + -0.6 137,463 + -0.1 137,606 + +0.6 137,607 - +0.0 137,607 - +1.0 137,607 - -0.3 137,607 - -0.3 137,610 + -0.4 137,610 + +0.1 137,611 - +0.1 137,611 - +0.5 137,611 - -0.3 137,611 - +0.5 137,614 - +0.3 137,637 + -1.0 137,638 - +0.6 137,638 - -0.6 137,638 - +0.5 137,638 - +0.4 137,741 + -0.2 137,742 - +0.3 137,742 - +0.6 137,742 - +0.3 137,799 + EX31_RS12330 0.22 -0.3 137,800 - EX31_RS12330 0.23 -0.0 137,800 - EX31_RS12330 0.23 -0.6 137,800 - EX31_RS12330 0.23 +0.0 137,866 - EX31_RS12330 0.39 +0.4 137,866 - EX31_RS12330 0.39 -0.3 137,889 - EX31_RS12330 0.44 -0.5 137,890 + EX31_RS12330 0.45 -0.9 137,890 + EX31_RS12330 0.45 +0.2 137,890 + EX31_RS12330 0.45 -0.2 137,890 + EX31_RS12330 0.45 -0.8 137,890 + EX31_RS12330 0.45 -0.4 137,890 + EX31_RS12330 0.45 -1.0 137,890 + EX31_RS12330 0.45 -0.2 137,891 - EX31_RS12330 0.45 -0.5 137,891 - EX31_RS12330 0.45 -1.2 137,891 - EX31_RS12330 0.45 -2.3 137,922 + EX31_RS12330 0.52 +0.8 137,931 + EX31_RS12330 0.55 -0.0
Or see this region's nucleotide sequence