Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10020 and EX31_RS10025 are separated by 163 nucleotides EX31_RS10025 and EX31_RS10030 are separated by 278 nucleotides EX31_RS10030 and EX31_RS10035 are separated by 297 nucleotides
EX31_RS10020: EX31_RS10020 - YciI family protein, at 211,532 to 211,828
_RS10020
EX31_RS10025: EX31_RS10025 - hypothetical protein, at 211,992 to 212,282
_RS10025
EX31_RS10030: EX31_RS10030 - YciY family protein, at 212,561 to 212,743
_RS10030
EX31_RS10035: EX31_RS10035 - cardiolipin synthase, at 213,041 to 214,501
_RS10035
Position (kb) 212
213 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 211.750 kb on - strand, within EX31_RS10020 at 211.787 kb on + strand, within EX31_RS10020 at 211.822 kb on - strand at 212.024 kb on - strand, within EX31_RS10025 at 212.081 kb on - strand, within EX31_RS10025 at 212.131 kb on + strand, within EX31_RS10025 at 212.132 kb on - strand, within EX31_RS10025 at 212.164 kb on - strand, within EX31_RS10025 at 212.209 kb on - strand, within EX31_RS10025 at 212.366 kb on - strand at 212.392 kb on - strand at 212.398 kb on + strand at 212.398 kb on + strand at 212.398 kb on + strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.399 kb on - strand at 212.468 kb on + strand at 212.469 kb on - strand at 212.549 kb on - strand at 212.578 kb on - strand at 212.578 kb on - strand at 212.578 kb on - strand at 212.578 kb on - strand at 212.578 kb on - strand at 212.589 kb on + strand, within EX31_RS10030 at 212.590 kb on - strand, within EX31_RS10030 at 212.638 kb on - strand, within EX31_RS10030 at 212.642 kb on - strand, within EX31_RS10030 at 212.703 kb on - strand, within EX31_RS10030 at 212.836 kb on + strand at 212.837 kb on - strand at 212.837 kb on - strand at 212.948 kb on + strand at 212.949 kb on - strand at 213.000 kb on - strand at 213.173 kb on + strand at 213.203 kb on + strand, within EX31_RS10035 at 213.737 kb on + strand, within EX31_RS10035 at 213.737 kb on + strand, within EX31_RS10035
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 211,750 - EX31_RS10020 0.73 -1.2 211,787 + EX31_RS10020 0.86 +0.8 211,822 - -1.7 212,024 - EX31_RS10025 0.11 +0.2 212,081 - EX31_RS10025 0.31 -1.0 212,131 + EX31_RS10025 0.48 -1.9 212,132 - EX31_RS10025 0.48 -0.2 212,164 - EX31_RS10025 0.59 -1.2 212,209 - EX31_RS10025 0.75 -0.1 212,366 - +2.0 212,392 - +0.2 212,398 + -0.7 212,398 + +0.9 212,398 + -0.4 212,399 - -0.8 212,399 - +0.8 212,399 - -0.2 212,399 - +0.8 212,399 - +0.5 212,399 - -0.4 212,399 - -0.7 212,399 - +1.8 212,399 - -0.2 212,468 + +0.2 212,469 - -0.4 212,549 - +1.4 212,578 - +0.2 212,578 - -0.9 212,578 - -0.2 212,578 - +0.8 212,578 - -1.1 212,589 + EX31_RS10030 0.15 -2.2 212,590 - EX31_RS10030 0.16 +0.1 212,638 - EX31_RS10030 0.42 -1.0 212,642 - EX31_RS10030 0.44 -0.2 212,703 - EX31_RS10030 0.78 -0.6 212,836 + +0.5 212,837 - +0.9 212,837 - -0.7 212,948 + +1.1 212,949 - -1.2 213,000 - +1.2 213,173 + +0.5 213,203 + EX31_RS10035 0.11 +2.8 213,737 + EX31_RS10035 0.48 +2.1 213,737 + EX31_RS10035 0.48 +2.5
Or see this region's nucleotide sequence