Strain Fitness in Rahnella sp. WP5 around EX31_RS09590

Experiment: L-Glutamine (N); RCH2 media; no shaking

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS09575 and EX31_RS09580 overlap by 8 nucleotidesEX31_RS09580 and EX31_RS09585 overlap by 1 nucleotidesEX31_RS09585 and EX31_RS09590 overlap by 4 nucleotidesEX31_RS09590 and EX31_RS09595 are separated by 167 nucleotidesEX31_RS09595 and EX31_RS09600 are separated by 3 nucleotides EX31_RS09575: EX31_RS09575 - flagellar motor switch protein FliM, at 119,491 to 120,513 _RS09575 EX31_RS09580: EX31_RS09580 - flagellar motor switch protein FliN, at 120,506 to 120,922 _RS09580 EX31_RS09585: EX31_RS09585 - flagellar biosynthetic protein FliO, at 120,922 to 121,359 _RS09585 EX31_RS09590: EX31_RS09590 - flagellar type III secretion system pore protein FliP, at 121,356 to 122,138 _RS09590 EX31_RS09595: EX31_RS09595 - flagellar biosynthesis protein FliQ, at 122,306 to 122,575 _RS09595 EX31_RS09600: EX31_RS09600 - flagellar type III secretion system protein FliR, at 122,579 to 123,364 _RS09600 Position (kb) 121 122 123Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 120.431 kb on + strandat 120.431 kb on + strandat 120.442 kb on + strandat 120.488 kb on + strandat 120.488 kb on + strandat 120.489 kb on - strandat 120.520 kb on + strandat 120.722 kb on - strand, within EX31_RS09580at 120.860 kb on + strand, within EX31_RS09580at 120.861 kb on - strand, within EX31_RS09580at 120.901 kb on + strandat 120.901 kb on + strandat 120.901 kb on + strandat 120.902 kb on - strandat 120.952 kb on + strandat 120.952 kb on + strandat 121.035 kb on + strand, within EX31_RS09585at 121.148 kb on + strand, within EX31_RS09585at 121.243 kb on - strand, within EX31_RS09585at 121.435 kb on + strand, within EX31_RS09590at 121.618 kb on + strand, within EX31_RS09590at 121.725 kb on + strand, within EX31_RS09590at 121.737 kb on + strand, within EX31_RS09590at 121.921 kb on - strand, within EX31_RS09590at 122.008 kb on + strand, within EX31_RS09590at 122.008 kb on + strand, within EX31_RS09590at 122.305 kb on + strandat 122.532 kb on - strand, within EX31_RS09595at 122.549 kb on - strandat 122.561 kb on + strandat 122.562 kb on - strandat 122.562 kb on - strandat 122.562 kb on - strandat 122.562 kb on - strandat 122.624 kb on + strandat 122.624 kb on + strandat 122.625 kb on - strandat 122.728 kb on + strand, within EX31_RS09600at 122.812 kb on + strand, within EX31_RS09600at 122.918 kb on + strand, within EX31_RS09600at 122.919 kb on - strand, within EX31_RS09600at 123.114 kb on + strand, within EX31_RS09600

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking
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120,431 + -1.5
120,431 + -0.4
120,442 + -1.5
120,488 + +0.1
120,488 + -1.4
120,489 - +0.8
120,520 + -0.2
120,722 - EX31_RS09580 0.52 -1.0
120,860 + EX31_RS09580 0.85 -0.0
120,861 - EX31_RS09580 0.85 +0.8
120,901 + -1.0
120,901 + -0.2
120,901 + -2.2
120,902 - -3.1
120,952 + -0.2
120,952 + -0.1
121,035 + EX31_RS09585 0.26 -0.2
121,148 + EX31_RS09585 0.52 -2.2
121,243 - EX31_RS09585 0.73 -1.0
121,435 + EX31_RS09590 0.10 +0.4
121,618 + EX31_RS09590 0.33 -1.6
121,725 + EX31_RS09590 0.47 -0.4
121,737 + EX31_RS09590 0.49 -1.2
121,921 - EX31_RS09590 0.72 -0.6
122,008 + EX31_RS09590 0.83 -2.0
122,008 + EX31_RS09590 0.83 +0.1
122,305 + -0.2
122,532 - EX31_RS09595 0.84 -0.8
122,549 - -1.2
122,561 + -0.2
122,562 - -0.2
122,562 - -0.6
122,562 - -0.5
122,562 - +0.4
122,624 + -1.7
122,624 + -0.4
122,625 - -1.2
122,728 + EX31_RS09600 0.19 -1.0
122,812 + EX31_RS09600 0.30 -3.2
122,918 + EX31_RS09600 0.43 -0.8
122,919 - EX31_RS09600 0.43 -0.4
123,114 + EX31_RS09600 0.68 +1.8

Or see this region's nucleotide sequence