Experiment: L-Glutamine (N); RCH2 media; no shaking
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09455 and EX31_RS09460 are separated by 375 nucleotides EX31_RS09460 and EX31_RS09465 are separated by 14 nucleotides EX31_RS09465 and EX31_RS09470 overlap by 26 nucleotides
EX31_RS09455: EX31_RS09455 - FliC/FljB family flagellin, at 100,344 to 101,624
_RS09455
EX31_RS09460: EX31_RS09460 - flagellar filament capping protein FliD, at 102,000 to 103,436
_RS09460
EX31_RS09465: EX31_RS09465 - flagellar export chaperone FliS, at 103,451 to 103,888
_RS09465
EX31_RS09470: EX31_RS09470 - flagella biosynthesis regulatory protein FliT, at 103,863 to 104,225
_RS09470
Position (kb) 101
102
103
104 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 101.130 kb on - strand, within EX31_RS09455 at 101.160 kb on - strand, within EX31_RS09455 at 101.215 kb on - strand, within EX31_RS09455 at 101.215 kb on - strand, within EX31_RS09455 at 101.248 kb on - strand, within EX31_RS09455 at 101.248 kb on - strand, within EX31_RS09455 at 101.352 kb on - strand, within EX31_RS09455 at 101.358 kb on + strand, within EX31_RS09455 at 101.358 kb on + strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.359 kb on - strand, within EX31_RS09455 at 101.411 kb on + strand, within EX31_RS09455 at 101.412 kb on - strand, within EX31_RS09455 at 101.456 kb on + strand, within EX31_RS09455 at 101.457 kb on - strand, within EX31_RS09455 at 101.612 kb on + strand at 101.613 kb on - strand at 101.630 kb on + strand at 101.630 kb on + strand at 101.696 kb on + strand at 101.726 kb on + strand at 101.727 kb on - strand at 101.751 kb on + strand at 101.764 kb on - strand at 101.774 kb on - strand at 101.774 kb on - strand at 101.774 kb on - strand at 101.795 kb on - strand at 101.811 kb on + strand at 101.811 kb on + strand at 101.857 kb on + strand at 101.857 kb on + strand at 101.858 kb on - strand at 101.858 kb on - strand at 101.858 kb on - strand at 101.859 kb on + strand at 101.860 kb on - strand at 101.863 kb on + strand at 101.863 kb on + strand at 101.863 kb on + strand at 101.863 kb on + strand at 101.958 kb on + strand at 101.958 kb on + strand at 101.959 kb on - strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.968 kb on + strand at 101.995 kb on + strand at 102.000 kb on - strand at 102.018 kb on - strand at 102.028 kb on + strand at 102.028 kb on + strand at 102.028 kb on + strand at 102.028 kb on + strand at 102.029 kb on - strand at 102.046 kb on + strand at 102.126 kb on + strand at 102.212 kb on + strand, within EX31_RS09460 at 102.212 kb on + strand, within EX31_RS09460 at 102.294 kb on + strand, within EX31_RS09460 at 102.294 kb on + strand, within EX31_RS09460 at 102.389 kb on + strand, within EX31_RS09460 at 102.546 kb on + strand, within EX31_RS09460 at 102.629 kb on + strand, within EX31_RS09460 at 102.640 kb on - strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.641 kb on + strand, within EX31_RS09460 at 102.764 kb on + strand, within EX31_RS09460 at 102.821 kb on + strand, within EX31_RS09460 at 102.822 kb on - strand, within EX31_RS09460 at 102.864 kb on + strand, within EX31_RS09460 at 102.864 kb on + strand, within EX31_RS09460 at 102.864 kb on + strand, within EX31_RS09460 at 102.865 kb on - strand, within EX31_RS09460 at 102.881 kb on + strand, within EX31_RS09460 at 102.881 kb on + strand, within EX31_RS09460 at 103.058 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.153 kb on + strand, within EX31_RS09460 at 103.229 kb on + strand, within EX31_RS09460 at 103.287 kb on + strand, within EX31_RS09460 at 103.287 kb on + strand, within EX31_RS09460 at 103.287 kb on + strand, within EX31_RS09460 at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.412 kb on + strand at 103.413 kb on - strand at 103.413 kb on - strand at 103.435 kb on - strand at 103.437 kb on + strand at 103.438 kb on - strand at 103.450 kb on + strand at 103.450 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.454 kb on + strand at 103.455 kb on - strand at 103.455 kb on - strand at 103.456 kb on + strand at 103.456 kb on + strand at 103.456 kb on + strand at 103.456 kb on + strand at 103.457 kb on - strand at 103.478 kb on + strand at 103.579 kb on + strand, within EX31_RS09465 at 103.579 kb on + strand, within EX31_RS09465 at 103.668 kb on + strand, within EX31_RS09465 at 103.668 kb on + strand, within EX31_RS09465 at 103.695 kb on + strand, within EX31_RS09465 at 103.718 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.724 kb on + strand, within EX31_RS09465 at 103.725 kb on - strand, within EX31_RS09465 at 103.725 kb on - strand, within EX31_RS09465 at 103.727 kb on - strand, within EX31_RS09465 at 103.838 kb on + strand, within EX31_RS09465 at 103.839 kb on - strand, within EX31_RS09465 at 103.839 kb on - strand, within EX31_RS09465 at 103.839 kb on - strand, within EX31_RS09465 at 103.859 kb on + strand at 103.861 kb on + strand at 103.861 kb on + strand at 103.878 kb on + strand at 103.878 kb on + strand at 103.878 kb on + strand at 103.980 kb on + strand, within EX31_RS09470 at 103.980 kb on + strand, within EX31_RS09470 at 103.980 kb on + strand, within EX31_RS09470 at 103.981 kb on - strand, within EX31_RS09470 at 104.072 kb on + strand, within EX31_RS09470 at 104.172 kb on - strand, within EX31_RS09470 at 104.178 kb on + strand, within EX31_RS09470 at 104.179 kb on - strand, within EX31_RS09470 at 104.179 kb on - strand, within EX31_RS09470 at 104.256 kb on + strand at 104.311 kb on + strand at 104.311 kb on + strand at 104.312 kb on - strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.338 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.344 kb on + strand at 104.368 kb on + strand at 104.402 kb on + strand at 104.402 kb on + strand at 104.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); RCH2 media; no shaking remove 101,130 - EX31_RS09455 0.61 -0.4 101,160 - EX31_RS09455 0.64 -0.8 101,215 - EX31_RS09455 0.68 +1.8 101,215 - EX31_RS09455 0.68 -1.2 101,248 - EX31_RS09455 0.71 +0.8 101,248 - EX31_RS09455 0.71 +0.6 101,352 - EX31_RS09455 0.79 -1.0 101,358 + EX31_RS09455 0.79 +1.1 101,358 + EX31_RS09455 0.79 -1.2 101,359 - EX31_RS09455 0.79 +1.3 101,359 - EX31_RS09455 0.79 +1.3 101,359 - EX31_RS09455 0.79 -1.0 101,359 - EX31_RS09455 0.79 +0.2 101,359 - EX31_RS09455 0.79 -0.0 101,411 + EX31_RS09455 0.83 -1.8 101,412 - EX31_RS09455 0.83 -1.6 101,456 + EX31_RS09455 0.87 +0.2 101,457 - EX31_RS09455 0.87 -1.8 101,612 + -2.2 101,613 - -0.8 101,630 + -0.6 101,630 + -2.6 101,696 + -1.0 101,726 + -1.1 101,727 - -0.2 101,751 + -1.2 101,764 - -1.3 101,774 - -1.7 101,774 - -1.1 101,774 - +0.0 101,795 - +0.8 101,811 + -0.2 101,811 + -0.1 101,857 + -0.2 101,857 + +0.3 101,858 - -0.6 101,858 - -0.6 101,858 - +1.2 101,859 + +0.8 101,860 - -1.1 101,863 + -0.2 101,863 + -0.5 101,863 + +0.3 101,863 + +0.5 101,958 + -0.6 101,958 + -0.7 101,959 - -2.5 101,968 + +0.2 101,968 + -1.1 101,968 + -1.3 101,968 + +0.4 101,968 + +0.0 101,995 + -0.0 102,000 - +1.4 102,018 - -0.4 102,028 + -0.9 102,028 + -1.9 102,028 + +0.5 102,028 + -0.2 102,029 - -1.0 102,046 + +2.3 102,126 + -0.8 102,212 + EX31_RS09460 0.15 -0.2 102,212 + EX31_RS09460 0.15 -2.0 102,294 + EX31_RS09460 0.20 -1.1 102,294 + EX31_RS09460 0.20 +1.2 102,389 + EX31_RS09460 0.27 +0.2 102,546 + EX31_RS09460 0.38 -2.9 102,629 + EX31_RS09460 0.44 -2.9 102,640 - EX31_RS09460 0.45 -0.2 102,641 + EX31_RS09460 0.45 -2.4 102,641 + EX31_RS09460 0.45 -2.9 102,641 + EX31_RS09460 0.45 +0.2 102,641 + EX31_RS09460 0.45 +0.2 102,764 + EX31_RS09460 0.53 +1.8 102,821 + EX31_RS09460 0.57 -0.8 102,822 - EX31_RS09460 0.57 -1.2 102,864 + EX31_RS09460 0.60 -0.8 102,864 + EX31_RS09460 0.60 -0.6 102,864 + EX31_RS09460 0.60 -1.2 102,865 - EX31_RS09460 0.60 -1.2 102,881 + EX31_RS09460 0.61 -1.5 102,881 + EX31_RS09460 0.61 -0.7 103,058 + EX31_RS09460 0.74 -0.8 103,153 + EX31_RS09460 0.80 -1.1 103,153 + EX31_RS09460 0.80 -1.0 103,153 + EX31_RS09460 0.80 -0.3 103,153 + EX31_RS09460 0.80 +0.2 103,153 + EX31_RS09460 0.80 +0.4 103,153 + EX31_RS09460 0.80 +0.2 103,229 + EX31_RS09460 0.86 -0.6 103,287 + EX31_RS09460 0.90 +1.4 103,287 + EX31_RS09460 0.90 -2.0 103,287 + EX31_RS09460 0.90 +0.8 103,412 + -1.4 103,412 + -0.6 103,412 + -0.8 103,412 + -2.3 103,412 + +0.8 103,412 + +0.4 103,412 + -2.1 103,412 + -1.8 103,413 - +0.2 103,413 - -1.4 103,435 - -1.7 103,437 + -0.6 103,438 - -0.9 103,450 + -1.0 103,450 + -1.7 103,454 + -0.8 103,454 + -1.5 103,454 + -0.1 103,454 + -0.5 103,454 + -1.4 103,454 + -0.0 103,454 + -0.9 103,454 + -2.6 103,454 + +0.1 103,454 + -2.1 103,454 + -0.8 103,454 + -1.4 103,454 + +0.2 103,454 + -0.2 103,455 - -0.6 103,455 - -0.0 103,456 + -2.1 103,456 + -1.2 103,456 + -1.7 103,456 + -1.5 103,457 - +0.2 103,478 + -0.4 103,579 + EX31_RS09465 0.29 +1.0 103,579 + EX31_RS09465 0.29 +1.4 103,668 + EX31_RS09465 0.50 -1.7 103,668 + EX31_RS09465 0.50 +1.8 103,695 + EX31_RS09465 0.56 -1.9 103,718 + EX31_RS09465 0.61 -0.6 103,724 + EX31_RS09465 0.62 -0.9 103,724 + EX31_RS09465 0.62 -0.4 103,724 + EX31_RS09465 0.62 -2.1 103,724 + EX31_RS09465 0.62 +0.2 103,724 + EX31_RS09465 0.62 -1.7 103,724 + EX31_RS09465 0.62 -2.3 103,724 + EX31_RS09465 0.62 -2.6 103,725 - EX31_RS09465 0.63 -1.0 103,725 - EX31_RS09465 0.63 +0.2 103,727 - EX31_RS09465 0.63 -1.0 103,838 + EX31_RS09465 0.88 -0.5 103,839 - EX31_RS09465 0.89 -2.1 103,839 - EX31_RS09465 0.89 +0.8 103,839 - EX31_RS09465 0.89 -0.8 103,859 + -1.4 103,861 + -0.9 103,861 + -0.6 103,878 + -1.4 103,878 + +1.1 103,878 + -0.6 103,980 + EX31_RS09470 0.32 +0.8 103,980 + EX31_RS09470 0.32 +0.4 103,980 + EX31_RS09470 0.32 -1.0 103,981 - EX31_RS09470 0.33 +2.4 104,072 + EX31_RS09470 0.58 -0.3 104,172 - EX31_RS09470 0.85 -0.8 104,178 + EX31_RS09470 0.87 +0.8 104,179 - EX31_RS09470 0.87 -0.4 104,179 - EX31_RS09470 0.87 -0.8 104,256 + +2.8 104,311 + +0.3 104,311 + -0.0 104,312 - +0.2 104,338 + -0.0 104,338 + -0.2 104,338 + -0.6 104,338 + -0.6 104,338 + -2.3 104,344 + +0.8 104,344 + -1.9 104,344 + -0.8 104,344 + -1.6 104,344 + +1.2 104,344 + -1.4 104,368 + +1.1 104,402 + -0.4 104,402 + -0.5 104,403 - -0.4
Or see this region's nucleotide sequence